JPH03176661A - Sample adjusting device and blood cell measuring instrument - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to an apparatus for performing biochemical analysis or hematology analysis, and particularly for sample preparation in which a reagent is added to a sample or a dilution operation is performed multiple times in total. The present invention relates to a device and a blood cell measuring device used after sample preparation.

[Conventional technology]

As a conventional sample preparation device, US, Patent
No.

4.451,433rAutomatic Chemi
An example of this is a discrete adjustment device described in Cal AnalyzerJ.

The automatic biochemistry analyzer is a device that analyzes protein components in plasma. Multiple reagents are sequentially added to one sample at intervals of one hour, and the reaction process is measured by measuring the absorbance of the mixture of reagents and sample. It can be understood as a change in the amount of water or a change in ions.

To achieve this, discrete conditioning equipment is required, including a pipettor for taking the sample and dispensing it into one reaction vessel, a pipettor for adding the reagent to it, and a pipettor for stirring the sample and reagent in the reaction vessel. It is equipped with a mechanism for cleaning pipetters and containers. Each pipettor or mechanism is powered by many motors and operates under complex computer control.

Next, as a sample preparation device for blood cell measuring devices,
- "Automatic blood analyzer" disclosed in Publication No. 16667
sample dilution, hemolysis flow system is increased. In this system, a fixed amount of blood is collected and diluted in two stages, and a hemolytic agent is added to the diluted blood to cause hemolysis. To this end, there are two complex quantitative sampling valves, a dilution stirring chamber, and a number of electromagnetically actuated valves. In addition, many tubes are intricately arranged to transfer samples and reagents between them.

[Problem to be solved by the invention]

The sample preparation device of the automatic biochemical analyzer described above is
For sample preparation, pipetters for samples and reagents,
This method requires many elements such as a reaction vessel, a stirring mechanism, and a cleaning mechanism, and the drive mechanism for each element is also complicated, resulting in a problem that the entire apparatus becomes very expensive.

In addition, in the two-stage dilution or flow system that performs dilution and hemolytic agent addition in a blood cell measuring device, there are long tubes connecting many valve bodies and the dilution stirring chamber, and a quantitative sampling valve with a complicated structure.
When blood passes through these, there is significant staining due to blood protein components and adhesion and retention of blood cells, which causes problems such as mutual contamination between samples and carryover that greatly affects measurement accuracy.

A first object of the present invention is to provide a sample preparation device that is simple in structure and inexpensive.

A second object of the present invention is to provide a blood cell measuring device that can significantly suppress cross-contamination between samples and carryover.

[Means to solve the problem]

In order to achieve the above object, the sample preparation device of the present invention adds various liquid reagents to a sample liquid to be analyzed to adjust the sample liquid. A first pipe line having a pore at one end, a branch part provided at the other end of the first pipe line, and a plurality of second pipe lines extending with one end connected to the branch part.
a conduit, an enlarged conduit provided at the other end of each of the second conduits, a third conduit extending with one end connected to a reduction port provided in each of the enlarged conduits, and the third conduit. It is characterized by comprising liquid suction and discharge means connected to the other ends of the passages.

Moreover, in order to achieve the above-mentioned first object, another sample preparation device of the present invention includes the above-mentioned first conduit.

A sample preparation device comprising a branch section, a second pipe, an expansion pipe, a third pipe, and a liquid suction/discharge means, a sample tank for storing a sample liquid prepared by the sample preparation device, and a liquid reagent. and a driving means for immersing the pore of the first conduit into the sample tank and the reagent tank.

The driving means includes a turning mechanism that turns the first pipe line and a lift IN! that moves the first pipe up and down. = Mechanism.

In order to achieve the above second object, the hemocytometer JI of the present invention
The II device includes a first pipe line having a pore at one end for sucking blood and various liquid reagents to be added to the blood, a branch part provided at the other end of the first pipe line, and a branch part provided at the other end of the first pipe line. a plurality of second conduits extending with their one ends connected; an enlarged tube section provided at the other end of each of the second conduits; and a plurality of second conduits extending with one end connected to a contraction port provided in each of the enlarged tube sections. a sample preparation device comprising a third pipe line and liquid suction/discharge means each connected to the other end of the third pipe line; a blood tank for storing the blood; and a reagent tank for storing the liquid reagent, respectively; a cleaning means for cleaning the outside of the first conduit; a sample receiving section for receiving the adjusted blood sample made of the blood and a liquid reagent mixed by the sample preparation device from the first conduit; and a downstream of the sample receiving section. a flow cell comprising a detection means for detecting blood cells in the adjusted blood sample; and the first conduit is connected to the blood tank and the reagent tank.

It is characterized by comprising a cleaning means and a driving means for moving the sample to the sample receiving section.

[Effect]

The sample preparation device of the present invention is operated as follows in the three adjustment processes for adjusting the sample liquid, namely, the reagent suction process, the sample liquid suction process, and the reagent'':i addition process. The following explanation will be given assuming that each of the discharge means and the third conduit and enlarged conduit sequentially connected thereto are filled with a working fluid which is also a cleaning fluid.

i) Reagent suction process First, the first of the plurality of liquid discharge suction means is operated to discharge the working liquid from the i-th enlarged conduit, through the i-th second conduit, through the branch part, and from the first conduit. The liquid suction and discharge means is actuated to suck a certain amount of air into the first conduit.

Then, the first pipe line is immersed in the first liquid reagent to be aspirated (hereinafter referred to as reagent), and the liquid suction and discharge means is operated to aspirate to aspirate the first reagent. The first reagent enters the first expanded conduit from the first conduit through the branch section, the first second conduit, and is stored in a certain amount.

Next, move on to aspiration of the second reagent. The second liquid suction and discharge means is operated to discharge, and the working fluid is discharged from the second enlarged conduit, the second conduit, the branch part, and the first conduit, and the working liquid is discharged from the first conduit that had entered the first conduit. Rinse out the reagent. The second liquid suction and discharge means is operated to suck a certain amount of air into the first pipeline.

Then, the first conduit is immersed in the second reagent, and the second liquid discharge suction means is operated to suck the second reagent. The second reagent is passed from the first conduit to the branch part, and then to the second reagent.
It passes through the conduit and enters the second enlarged conduit, where it is stored in a certain amount. During this second suction operation, when air passes through the first branch, the first liquid suction and discharge means is suctioned to draw the i-th reagent into the first second conduit, and the end of the first reagent is drawn into the first second conduit. Inject air between the and the branch. Hereinafter, the suction of the third and subsequent reagents is performed in the same manner as the suction of the second reagent. Thus, the first to nth reagents are each stored in a separate second conduit-expansion conduit.

ii) Sample liquid aspiration process After the predetermined n-th reagent has been added, the sample liquid is aspirated. In this process, first, the liquid suction and discharge means for the reagent to be added to the sample liquid first (this is set as the i-th one)
is discharged, and the reagent end in the i-th second conduit is moved through the branch to near the pore of the first conduit. The first conduit is then immersed in the sample liquid. The i-th liquid suction and discharge means is operated to suction, and a predetermined amount of the sample liquid is suctioned into the first conduit. The first conduit is then separated from the sample liquid surface.

iii) Reagent addition process The i-th liquid suction and discharge means is operated to suck again, and the sucked sample liquid is drawn into the i-th enlarged conduit. In the enlarged conduit, the area of the passage stage suddenly expands, so that the i-th reagent and the subsequent sample liquid come together and mix therein. This completes the first reagent addition.

The thus obtained mixed solution of the sample liquid and the i-th reagent is referred to as a first adjusted sample.

Begin the second reagent addition. The i-th liquid suction and discharge means is operated for discharge, and the first tI711! sample first
Move to the middle of the pipe. In this state, the next reagent to be added (
The liquid suction and discharge means for the j-th reagent) is operated to suction, and the first adjusted sample is aspirated into the j-th expansion tube section. At the same time, the i-th liquid suction and discharge means is operated to discharge, and the first adjusted sample continues to be sent to the j-th expansion tube section through the branch section and the j-th second tube section.

After a predetermined amount of the first adjusted sample has been sucked into the j-th expansion tube section, the j-th liquid suction and discharge means is stopped, the i-th liquid suction and discharge means is switched from discharge to suction operation, and The first adjusted sample located at is pulled back through the branch to the i-th second conduit. This pulling back is performed in order to cut off the rear end of the first adjusted sample sent to the j-th expansion tube section with air and quantify the amount thereof. Then, the j-th liquid suction and discharge means is operated to suck, and all of the first adjusted sample in the j-th second pipe line is sucked into the j-th expansion tube section, and the j-th reagent and the first adjusted sample are Mix the second
Make an adjusted sample.

At this point, the i-th liquid suction and discharge means is operated to discharge the cleaning liquid, and the remaining first i! l! The prepared sample may be flushed out of the pores of the first conduit and the process may proceed to aspirate the necessary reagents.

From the third time onward, reagent addition is performed in the same manner as in the second time.

Another sample preparation device of the present invention is a combination of a sample tank for storing a sample liquid, a reagent tank for storing a reagent, and a drive means for the first pipe line in the above-described sample preparation device, so that its operation is controlled by the sample liquid or the reagent tank. If we add the explanation that when the first pipe line is immersed in the reagent, the first pipe line is moved to the sample tank or the reagent tank by the driving means, the explanation will be the same as the above-mentioned sample preparation device, so the explanation will be omitted here. do. Further, the turning mechanism positions the first pipe line above the sample tank or the reagent tank, and then the elevating mechanism moves the first pipe line toward and away from each tank.

Next, the operation of the blood cell measuring device of the present invention will be explained. The sample liquid to be measured is blood. For example, in the case of two-stage dilution of blood, saline is used as the first reagent and the second reagent. Also, when adding a hemolytic agent to blood, add it as a second reagent.

Physiological saline is used as the working fluid for each liquid suction and discharge means.The operation of the sample preparation device constituting this blood measuring device is the same as that of the sample preparation device described above, and therefore the description thereof will be omitted here.

After conditioning the blood, the sample conditioning device discharges the final adjusted blood sample (referred to as blood sample) from the first pipe line to the sample receiving section of the flow cell, and detecting means downstream from the sample receiving section. measures blood cells in a blood sample.

Then sample! l! ! The first pipe line of the cleaning device is moved to the cleaning means by the driving means, and the first pipe line is moved by the cleaning means to the cleaning means.
Clean the outside of the conduit. Further, each liquid suction and discharge means is operated to discharge, and the cleaning liquid is discharged from the first conduit to wash away the adjusted blood, blood sample, etc. remaining in the apparatus.

〔Example〕

Embodiments of the present invention will be described below with reference to the drawings.

FIG. 1 is a block diagram of the sample adjustment device, and FIG. 2 is its operation time chart. This device is a device for mixing and reacting n types of reagents and samples one after another.

In FIG. 1, a thin tube 1 serving as a first conduit is for aspirating and discharging reagents and samples. The thin tube 1
There is a branch pipe 2 as a branch part at the upper end of the pipe. From the branch pipe 2, n intermediate pipes 3-1 to 3-n as second pipes extend, and at each end there is a mixing chamber 4, which is an enlarged pipe section that is wide at the bottom and narrows toward the top. -1 to 4-n are
It is provided. From the upper part of each mixing chamber 4-1 to 4-n.

Hydraulic fluid pipes 5-1 to 5-n as third pipes extend. The hydraulic fluid pipes 5-1 to 5-n are connected to syringes 6-1 to 6-n, which are liquid suction and discharge means, respectively. Mixing chambers 4-1 to 4- from each syringe 6-1 to 6-n
The working fluids 7-1 to 7-n, which also serve as cleaning fluids, are filled up to n. In addition, n reagent containers 9-1 to 9-n are arranged near the capillary, and n types of reagents 8-1 to 8-n are stored in each container. Also arranged is a sample container 10, which contains a sample 11.

The sample preparation device configured as described above is operated as follows.

1) Reagent suction process First, the syringe barrel 6-1 is operated to discharge, and the working liquid 7-↓ is discharged from the mixing chamber through the intermediate pipe 3-1, the branch pipe 2, and the thin tube 1. Thereafter, the syringe barrel 6-1 is operated to suck, and a certain amount of air is sucked into the thin tube l. Then, the thin tube 1 is immersed in the reagent 8-1, and a suction operation is performed using the syringe barrel 6-↓.
Aspirate the reagent 8-1 until it fills the mixing chamber 4-1.

Next, the syringe barrel 6-2 is discharged, and the working fluid 7-2 is passed from the mixing chamber 4-2 through the intermediate pipe 3-2 and the branch pipe 2 into the narrow 'f'.
1 and wash away the reagent 8-1 remaining in the tube 41. The syringe barrel 6-2 is suctioned to pull the working fluid 7-2 in the thin tube 1 back to the front of the branch pipe 2 (into the intermediate pipe 3-2), and the syringe barrel 6-1 is sucked in a small amount at this opening. , slightly draw the tip of the reagent 8-1 into the intermediate pipe 3-1.

In this way, since air is present between the tip of the reagent 8-1 and the branch tube 2, the reagent 8-1 does not come into contact with other reagents in the branch tube 2. Furthermore, since there is an air layer at both ends of the reagent 8-1, the amount of the reagent 8-1 can be quantified.

In this state, move on to aspiration of the second reagent. The syringe barrel 6-2 is discharged, and the hydraulic fluid 7 returns to the front of the branch pipe 2.
-2 to a predetermined position in the thin tube 1. Then dip the capillary top into the second reagent 8-2. The syringe barrel 6-2 is actuated to suck the reagent 8-2 until it fills the mixing chamber 4-2. After that, cleaning of the reagent 8-2 remaining in the thin tube 1 and quantitative determination of the reagent 8-2 are carried out between the syringe barrel 6-2 and the syringe barrel 6-2 in the same manner as above.
-3 suction and discharge operation is performed. For the third and subsequent reagent aspiration,
This is done by repeating the same operation as in the second case.

ii) Sample aspiration process After the predetermined n-th reagent aspiration is completed, sample aspiration is started. In this example, it is assumed that the reagents are mixed and reacted in order starting from reagent 8-K. first. Syringe barrel 6-
1 is discharged, and the reagent 8-↓, which had returned to the front of the branch tube 2, is advanced to near the outlet on the thin tube. In this state, the thin tube is moved to the sample container 10 and immersed into the sample 11. The syringe barrel 6-1 is operated for suction, and a certain amount of the sample 11 is sucked into the thin tube 1. Thereafter, the thin tube 1 is pulled up from the sample liquid surface. The syringe barrel 6-↓ is operated to suck again, and the entire sample sample 1 that has been sucked is introduced into the mixing chamber 4-1.

1ii) Reagent addition process When the sample 11 is introduced into the mixing chambers 4 to 1,
Since the inlet portion of the reagent 8-1 has a rapidly expanding shape, a vortex is generated near the inlet, and the sample 11 and the reagent 8-1 are mixed. If the liquid is not mixed sufficiently after one introduction, perform the ejection and suction operations of the syringe barrel 6-1 several times to remove sample 1.
1 and reagent 8-1 to intermediate piping 3-1 and mixing chamber 4-.
1 to ensure complete mixing. Hereinafter, this mixed liquid will be referred to as the first U8 prepared sample.

When the mixing with the reagent 8-1 is completed, the process moves on to mixing a certain amount of the first adjustment sample and a certain amount of the reagent 8-2. First, the syringe barrel 6-1 is operated to discharge, and the first adjusted sample is advanced through the branch pipe 2 to the capillary tube E. At the same time that the liquid passes through the branch pipe 2, the suction operation of the syringe barrel 6-2 is started, and a predetermined amount of the ↓th adjusted sample is introduced into the mixing chamber 4-2 through the pipe 3-2. When it finishes.

The syringe barrel 6-1 is once sucked, the first adjusted sample remaining on the thin tube is returned to the intermediate pipe 3-1, and the rear end of the adjusted sample headed for the mixing chamber 4-2 is transferred to the branch pipe 2. At this point, cut at the air layer and quantify. Mixing chamber 4-
2 also has the same shape as the mixing chamber 4-1, and the first preparation sample and reagent 8-2 can be mixed in the same manner.

Hereinafter, different reagents will be mixed one after another in the same manner as above.

FIG. 2 shows a time chart of the above operations, focusing on the suction and discharge operations of each syringe barrel.

In the above process, all the reagents were first aspirated and then the sample 11 was aspirated, but the sample 11 may be aspirated immediately after the first reagent 8-1 is aspirated. sample 1
1 and reagent 8-1 are being mixed in the mixing chamber 4-1, reagent 8-2 is aspirated, and while the first prepared sample and reagent 8-2 are being mixed, reagent 8-2 is aspirated. If steps 3 and 3 are subsequently performed in this order, the overall sample preparation time can be significantly reduced compared to the case of the above embodiment.

In addition, the syringe barrels 6-1 to 6-n or the hydraulic fluid piping 5-1
If predetermined reagents are placed in ~5-n in advance, the reagent suction process can be skipped, and this case also greatly contributes to shortening the overall adjustment time.

Next, an embodiment of the blood cell measuring device of the present invention will be described with reference to FIGS. 3, 4, and 5. FIG. 3 is a detailed configuration diagram of the blood adjustment section, FIG. 4 is an overall diagram of the blood cell measuring device, and FIG. 5 is a time chart showing the operation of the device, centering on the movement of the syringe barrel.

First, the configuration of the sample adjustment section of this embodiment will be explained with reference to FIG. The sample adjustment section 12 is attached below the tip of an arm 13 that can be rotated and raised and lowered independently. Below the sample preparation section 12, blood, reagents,
A thin tube 14 for inhaling a preparation sample or the like is attached facing downward. A rubber spherical ring 34 is inserted into the tip of the thin tube 14 to facilitate connection with the sample receiver 36 of the flow cell 35. Sample receiver 3
6 is a suction port 3 for sucking the overflowing liquid.
7 is provided. In the sample adjustment section 12, there are 3
In Figure 3, the center is the mixing chamber 20 for diluting the first stage 11, the left is the mixing chamber 19 for diluting the second stage, and the right is the mixing chamber 21 for hemolysis. be. Each mixing chamber has a triangular shape with an enlarged bottom. Mixing chamber 20
A flow path 17 extends straight downward from the bottom, and is connected to the inner flow path of the thin tube 14 via a branch portion 15 along the way. From the branching part 15, other passages 16.18 extend to the left and right, curve upwards midway, and connect to the bottom of each mixing chamber 19.21 at the branching part 15.
, 18 are relatively thinner than the flow path 17.

Hydraulic fluid channels 22, 23, 24 extend upward from the apex of each mixing chamber 19.20, 21, respectively. These channels are filled with physiological saline 31°, 32°, and 33° as working fluid, respectively.

Next, the overall configuration of the blood cell measuring device will be explained using FIG. 4. Hydraulic fluid channels 22, 23, 24 in FIG. 3 pass through the arm 13 and are connected to syringe barrels 46, 45, 44, respectively. The syringe barrels 46, 45, and 44 can each independently perform suction and discharge operations. Below the orbit of the arm 13, from the front, there are a sample container 38, a hemolytic agent container 40, a washing tank 42, a flow cell 35,
are arranged in this order. There is blood 3 in the sample container 38.
9. A hemolytic agent 41 is contained in a hemolytic agent container 40, and a cleaning liquid is sprayed from around the thin tube 14 in a cleaning tank 42.

The flow cell is provided with an irradiation light source 47 for measurement and a detector 48 that captures signals from blood cells.

The blood cell measuring device configured as described above operates as follows.

i) Cleaning process First, the arm 13 is rotated and lowered into the cleaning tank 42 to clean the area around the thin tube 14. At the same time syringe 44.45.46
Both are discharged into the mixing chambers 19, 20, 21, and the flow path 1.
6, 17, 18, the channel in the thin tube 14 is flushed with physiological saline.

ii) Physiological saline/hemolytic agent inhalation process First, an operation is performed to fill the mixing chambers 19 and 20 with physiological saline for dilution. The syringe barrels 45 and 46 are operated as suction to inhale air. When air is sucked into the channels 16 and 17, the syringe barrel 44 starts discharging.

A physiological saline working fluid 33 is sent into the channels 16 and 17.

When a certain amount of this physiological saline fills each of the mixing chambers 19 and 20, the operation of the syringe 44 is stopped, and a little later the syringe 45 is opened.
．． The operation of 46 is stopped. By doing this, the flow path 16.
Air enters the portion of 17 that connects to branch portion 15.

In this state, the flow path within the thin tube 14 is filled with air.

Next, the suction operation of the hemolytic agent is started. The arm 13 is rotated to descend into the hemolytic agent container 40 and the capillary tube 14 is immersed in the hemolytic agent 14. The syringe barrel 44 is operated for suction, and the hemolytic agent 41 is inhaled until a certain amount of the hemolytic agent 41 fills the mixing chamber 21 .

Finally, a little air is sucked into the channel inside the capillary tube 4.

Sample suction process In the above state, the hemolytic agent fills the channel from the channel in the thin tube 14 to the branch 15 and channel 18. Also, from the first branch part 15 to the flow path king 6. ↓There is air up to a little point at 7, and the rest is filled with saline.

The arm 13 is pivoted and lowered to immerse the capillary tube 14 into the blood 39 in the sample container 38. The syringe barrel 44 is suctioned to suck a certain amount of blood from the channel in the thin tube 14 into the channel 8 and then stopped. Next, the suction operation of the syringe barrel 45 is started, a certain amount of blood 39 is sucked into the flow path F, and then the suction operation is stopped. Arm 1
3 to separate the thin tube 14 from the liquid level of the blood 39.

The syringe barrel 45 is again operated for suction, and the blood 39 filling the channel in the thin tube 14 and the channel 17 is sucked into the mixing chamber 20. During the above-mentioned inhalation, as soon as the blood 39 passes through the branch part 15, the syringe barrel 44 is operated to suck the blood 39 inside the flow path 18.
is drawn into the mixing chamber 21.

At this time, in the above operation, the physiological saline, hemolytic agent, and blood are inhaled after being segmented with air at both ends, so that quantitative properties such as the amount of blood sampled and the dilution ratio are not impaired.

iv) Mixing (dilution/hemolysis) process When the blood 39 is introduced into each mixing chamber 20.21, the flow path ↓
Rapidly expanding inflow from 7 and 18 promotes stirring. Several more times, the syringe barrel 44, 45 moves aspirating 1. The discharge operation is repeated alternately to cause the liquid to reciprocate between the mixing chamber and the middle of the channels 17 and 18. Now, in the second mixing chamber, hemolysis is completed and a blood sample containing only white blood cells is created. Further, in the mixing chamber 20, a first-stage blood dilution sample is prepared.

Next, the syringe barrel 45 is operated to discharge, and the - stage diluted blood sample is advanced from the branch part 15 to the flow path in the thin tube 14. At the same time, the syringe barrel 46 is operated to suck, and a certain amount of the first stage diluted blood sample is delivered to the mixing chamber 19. Inhale. The syringe barrel 45 is operated to suck, the -stage diluted blood sample is returned to the flow path 17, and the rear end of the single stage diluted blood sample sent to the mixing chamber 19 is cut off with air. Thereafter, the syringe barrel 46 is operated for suction, and the -stage diluted blood sample is introduced into the mixing chamber 19 and mixed evenly.

■) Measurement process: Rotate the arm 13 to remove the sample receiver 3 of the flow cell 35.
Move above 6. In this state, the syringe 46 is operated to discharge the two-stage diluted blood sample in the mixing chamber 9 into the thin tube 1.
4. Proceed to the tip of the inner channel.

Next, the arm 13 is lowered to connect the first spherical ring 34 and the sample receiver 36. The syringe barrel 46 is slowly discharged to send the two-stage diluted blood sample to the measurement point. When this measurement is completed, the arm 13 is slightly raised and the spherical ring 34 and sample receiver 36 are separated. Next, syringe 4
4 is started, the two-step diluted blood sample inside the capillary tube 4 is expelled, and the tip is filled with the hemolyzed sample. The expelled two-stage diluted blood sample is dropped into the sample receiver 36 and sucked in through the suction port 37. The arm 13 is lowered again to connect the thin tube ↑4 to the flow cell 35, and the syringe 44 is operated to discharge the hemolyzed sample to the measurement point.

vi) When the cleaning process measurement is completed, the arm 13 is moved to the cleaning tank 42. The outside of the thin tube 14 is washed with washing water sprayed from inside the washing tank 42. Further, the sample g preparation section 12 is washed by discharging the syringes 44, 45.degree. 46 at the same time, and flowing the respective working fluids 31, 32.degree. 33 from the mixing chamber into the respective channels. The area through which the blood sample passes is limited to the upper part of the sample adjustment section 2, and there are no valves or the like where the sample can easily accumulate, so cleaning can be done in a short time with a small amount of hydraulic fluid.

Although the above blood cell measuring device is an embodiment in which one sample adjustment section is provided, a plurality of sample adjustment sections may be provided.

By equipping multiple devices and adjusting samples one after another simultaneously, throughput (processing amount) can be greatly improved. Since the structure of the sample adjustment section is simple, a plurality of sample adjustment sections can be provided relatively easily.

In addition, like the sample preparation device described above, the sample preparation section of this example does not have any valve body, and the section through which blood passes is overwhelmingly shorter than that of the conventional sample preparation device. There is almost no dirt, blood cell retention, or adhesion. Therefore, cross-contamination with the sample to be measured next and carryover of blood cells, which causes carryover errors, can be significantly suppressed.

〔Effect of the invention〕

According to the present invention, the sample preparation device includes a first pipe line for aspirating/discharging sample liquid and various reagents, a plurality of expansion pipe parts each connected to the first pipe line through a branch part by a pipe line, and a liquid Since it is composed of a flow path group consisting of suction and discharge means, it can have a simple structure, and therefore the cost of the apparatus can be significantly reduced compared to conventional apparatuses.

In addition, the operation of adding a reagent to the sample liquid to adjust the sample liquid is performed by moving the sample liquid and reagent between the expansion tube section and the first pipe line using a liquid suction and discharge means, and the sample liquid and reagent are processed outside the apparatus. Since there are no blemishes, adjustments can be made cleanly, with high processing speed, and with high precision.

Furthermore, according to the present invention, since the blood cell adjustment device is constructed by combining the above-mentioned sample adjustment device and a flow cell for measuring blood cells, there is no valve body at all, and the flow path through which blood passes is overwhelmingly larger than that of conventional methods. Because of its short length, blood stains and adhesion and retention of blood cells can be significantly reduced.

Therefore, mutual contamination between samples and carryover can be suppressed, and as a result, measurement accuracy can be improved.

[Brief explanation of the drawing]

FIG. 1 is a block diagram of the sample conditioning device, FIG. 2 is an operation time chart of the sample conditioning device, FIG. 3 is a sectional view of the blood cell measuring device of the present invention, and FIG. 4 is an overall block diagram of the blood cell measuring device. FIG. 5 is an operation time chart of the blood cell measuring device. 1.14... Thin tube (first pipe line), 2... Branch pipe, 3
-1~3-n...Intermediate piping (second pipe line), 4-1~
4-n, 19-21...mixing chamber (expansion tube section). 5-1 to 5-n... Hydraulic fluid piping (third pipe line), 6
-1 to 6-n, 44 to 46... Syringe barrel (liquid suction and discharge means), 9-1 to 9-n... Reagent container, 10.38.
...Sample container, 12...Sample adjustment section, 13...Arm. 15... Branch part, 35... Flow cell, 36...
Sample receiver, 42...Cleaning tank. 48...Detector.

Claims (1)

[Scope of Claims] 1. In a sample preparation device for adding various liquid reagents to a sample liquid to be analyzed to prepare the sample liquid, a first a conduit, a branch provided at the other end of the first conduit, and a plurality of second conduits extending with one end connected to the branch;
a conduit, an enlarged conduit provided at the other end of each of the second conduits, a third conduit extending with one end connected to a reduction port provided in each of the enlarged conduit sections, and the third conduit. A sample preparation device comprising liquid suction and discharge means respectively connected to the other ends of the channels. 2. The sample preparation device according to claim 1, a sample tank for storing the sample liquid, a reagent tank for storing the liquid reagent, and a drive for immersing the pore of the first conduit into the sample tank and the reagent tank. A sample preparation device comprising: means. 3. The sample preparation device according to claim 2, wherein the driving means is comprised of a turning mechanism for turning the first conduit and a lifting mechanism for raising and lowering the first pipe. 4. A first conduit having a pore at one end for sucking blood and various liquid reagents to be added to the blood, a branch provided at the other end of the first conduit, and one end connected to the branch. a plurality of second conduits that connect and extend; an enlarged tube section provided at the other end of each of the second conduit sections; and a third tube section that extends and connects one end to a contraction port provided in each of the enlarged tube sections. a conduit, and the third
cleaning the outside of the first pipe; a sample preparation device comprising liquid suction and discharge means connected to the other ends of the pipes; a blood tank for storing the blood; a reagent tank for storing the liquid reagent; and cleaning the outside of the first pipe. a sample receiving section for receiving, from the first conduit, a prepared blood sample consisting of the blood and a liquid reagent mixed by the sample preparation device; A blood cell measuring device comprising: a flow cell comprising a detection means for detecting blood cells; and a drive means for moving the first conduit to the blood tank, reagent tank, cleaning means, and sample receiving section.