JPH0782011B2 - Sample preparation device and blood cell measurement device - Google Patents

Description

Description: TECHNICAL FIELD The present invention relates to an apparatus for biochemical analysis and blood cell analysis, and particularly to sample preparation in which a reagent is added to a sample or a dilution operation is performed a plurality of times in total. The present invention relates to an apparatus and a blood cell measuring apparatus used after sample preparation.

[Conventional technology]

As a conventional sample adjustment device, US.Patent No.4,451,4
33 A discrete type adjusting device described in “Automatic Chemical Analyzer” can be mentioned. An automatic biochemical analyzer is a device for analyzing protein components in plasma. Multiple reagents are sequentially added to one sample at a certain time, and the reaction process is performed by measuring the absorbance of the mixture of the reagent and sample. Considered as changes or changes in ions. In order to achieve this, the discrete type adjusting device has a pipettor for collecting a sample and discharging it into a reaction container, a pipetter for adding a reagent to the reaction container, and for agitating the sample and the reagent in the reaction container. It is equipped with a mechanism for cleaning pipettes and containers. Each pipettor and mechanism is driven by many motors and operates under complex computer control.

Next, as a sample adjusting device for a blood cell measuring device,
The sample dilution and hemolysis flow system of the "automatic blood analyzer" disclosed in Japanese Patent No. 16667 can be raised. In this system, the sampled blood is quantitatively sampled and diluted in two stages, and a hemolytic agent is added to the diluted blood to hemolyze. Therefore, there are two complicated quantitative sampling valves, and many dilution stirring chambers and electromagnetically operated valves are used. Also, many tubes are complicatedly piped in order to transfer samples and reagents between them.

[Problems to be Solved by the Invention]

The sample preparation device of the biochemical automatic analyzer described above,
Pipettes for samples and reagents for sample preparation,
Many elements such as a reaction vessel, a stirring mechanism, and a cleaning mechanism are required, and the driving mechanism for each element is complicated, resulting in a problem that the entire apparatus becomes very expensive.

Also, in a flow system for performing two-step dilution of a blood cell counter or dilution and addition of a hemolyzing agent, there are long tubes connecting many valve bodies and dilution stirring chambers and a quantitative sampling valve in a complicated structure.
When blood passes through these, there has been a problem that stains and blood cells due to blood protein components are markedly attached and retained, which causes cross-contamination between samples and carryover that greatly affects measurement accuracy.

A first object of the present invention is to provide a sample preparation device which is simple in structure and inexpensive.

A second object of the present invention is to provide a blood cell counter which can significantly suppress cross-contamination between samples and carryover.

[Means for Solving the Problems]

In order to achieve the above object, the sample preparation device of the present invention is a sample preparation device for adding various liquid reagents to a sample liquid to be analyzed to prepare the sample liquid, and aspirates the sample liquid and various reagents, respectively. A first conduit having a pore at one end, a branch portion provided at the other end of the first conduit, and a plurality of second pipes extending with one end connected to the branch portion.
A pipeline, an expansion pipeline provided at the other end of each of the second pipelines, a third pipeline extending at one end thereof by connecting one end to a reduction port provided at each of the expansion pipelines, and the third pipeline It is characterized in that it is composed of liquid suction and ejection means respectively connected to the other ends of the paths.

Further, in order to achieve the first object, another sample adjusting device of the present invention is the above-mentioned first conduit, branch portion, second conduit, expansion conduit, third conduit and liquid suction / discharge. And a sample tank for storing a sample solution adjusted by the sample adjusting apparatus, a reagent tank for storing a liquid reagent, and the sample pipe and the first conduit in the reagent tank. And a driving means for immersing the fine pores.

It is preferable that the driving means includes a turning mechanism that turns the first conduit and an elevating mechanism that raises and lowers the first conduit.

In order to achieve the above-mentioned second object, the blood cell counter of the present invention comprises a first conduit having pores at one end for sucking blood and various liquid reagents added to the blood, and the first conduit. Provided at the other end of the second pipe, a plurality of second conduits extending by connecting one end to the branch, an expansion pipe part provided at the other end of each second pipe, and the expansion pipe A sample adjusting device comprising a third conduit extending at one end connected to a reduction port provided in each part, and a liquid aspirating / discharging means respectively connected to the other end of the third conduit; A blood tank for storing, a reagent tank for storing the liquid reagent, a cleaning means for cleaning the outside of the first conduit, and a prepared blood sample composed of the blood and the liquid reagent mixed by the sample preparation device, Sample receiving part and the sample received from one conduit A flow cell which is provided downstream of the blood vessel part and which comprises a detection means for detecting blood cells in the adjusted blood sample; and a drive means for moving the first conduit to the blood tank, reagent tank, cleaning means and sample receiving part. It is characterized by being composed of.

[Action]

The sample preparation device of the present invention is operated as follows in three adjustment processes for adjusting the sample liquid, that is, in the reagent suction process, the sample liquid suction process, and the reagent addition process. The following description will be made assuming that each of the liquid suction / ejection means and the third pipe and the enlarged pipe that are sequentially connected thereto are filled with the working liquid that is also the cleaning liquid.

i) Reagent aspirating process First, the first of the plurality of liquid ejecting and sucking means is operated to eject the working liquid from the first expanded pipe line to the first second pipe line and from the first pipe line via the branch portion. The liquid suction / discharging means is caused to perform a suction operation to suck a fixed amount of air into the first conduit. Then I want to suck the first conduit 1
The first liquid reagent is soaked in the second liquid reagent (hereinafter, referred to as a reagent), and the liquid suction / discharging means is sucked to suck the first reagent.
The first reagent enters from the first conduit to the first expansion conduit via the branch portion, the first second conduit, and is stored in a fixed amount.

Next, move on to the suction of the second reagent. The second liquid suction / ejection means is caused to perform a discharge operation, and the working fluid is discharged from the second expanded pipe line to the second pipe line and from the first pipe line via the branch portion to enter the first pipe line. Rinse out the reagents from. The second liquid suction / discharge means is caused to perform a suction operation to suck a fixed amount of air into the first conduit. Wash the outside of the first line. then,
The first tube is dipped in the second reagent, and the second liquid discharge / intake means is sucked to suck the second reagent. The second reagent is branched from the first line to the second line via the second branch line.
The second expansion pipe is entered and a fixed amount is stored. During the second suction operation, when the air passes through the branch portion, the first liquid suction / discharge means is caused to perform the suction operation to draw the first reagent into the first second pipe line, and the end of the first reagent. Air between the branch and the branch. The suction of the third and subsequent reagents is performed in the same manner as the suction of the second reagent. Thus, the 1st to nth reagents are respectively stored in the separate second conduit-expansion conduit.

ii) Sample liquid suction process After the predetermined n-th reagent has been filled, the sample liquid is sucked. In this process, first, liquid suction / discharge means for the reagent to be added first to the sample liquid (this is the i-th)
Is discharged, and the reagent end in the i-th second pipeline is moved to near the pore of the first pipeline through the branch portion. First
Clean the outside of the line. Then, the first conduit is immersed in the sample solution. The i-th liquid suction / ejection means is caused to perform a suction operation to suck a predetermined amount of the sample liquid into the first conduit. Then the first
Separate the tubing from the sample surface.

iii) Reagent adding process The i-th liquid suction / ejection means is again actuated to draw the sucked sample liquid to the i-th expansion conduit. In the expansion conduit, since the passage step area suddenly expands, the i-th reagent and the sample liquid which follows thereafter are combined together and mixed therein. This completes the first reagent addition.
The mixed liquid of the sample liquid thus obtained and the i-th reagent is referred to as a first adjusted sample.

Start the second reagent addition. The i-th liquid suction / ejection means is caused to perform the ejection operation, and the first adjustment sample is moved partway along the first conduit. In this state, the liquid suction / ejection means of the reagent to be added next (referred to as the jth reagent) is caused to perform the suction operation, and the first adjustment sample is sucked into the jth expansion tube section. At the same time, the i-th liquid suction / discharging means is operated to discharge, and the first adjustment sample is continuously sent to the j-th expansion tube section through the branch section and the j-th second tube section.

After the first adjustment sample has been sucked into the j-th expansion tube portion by a predetermined amount, the j-th liquid suction / discharge means is stopped, and the i-th liquid suction / discharge means is changed from discharge to suction operation and the first pipeline The 1st adjustment sample in is pulled back to the i-th 2nd pipeline through a branch part. This pullback is performed to cut off the rear end of the first adjustment sample sent to the j-th expansion tube section with air and quantify the amount. Then, the j-th liquid sucking / discharging means is caused to perform a suction operation, and all the first adjustment sample in the j-th second conduit is sucked into the j-th expanding tube portion to obtain the j-th reagent and the first adjustment sample. To prepare a second conditioned sample.

At this point, the i-th liquid suction / discharging means is operated to discharge the cleaning liquid to wash away the remaining first adjustment sample from the pores of the first conduit, and if necessary, shift to the reagent suction process. Good.

After the third time, the reagent is added in the same manner as in the case of the second time.

Another sample preparation device of the present invention is a combination of the above-mentioned sample preparation device with a sample tank for storing a sample solution, a reagent tank for storing a reagent, and a driving means for the first conduit, so that the operation is performed by the sample solution or If the description that the first conduit is moved to the sample tank or the reagent tank by the driving means when the first conduit is immersed in the reagent is added, the description is omitted because it is similar to the description of the sample adjusting device described above. To do. The swivel mechanism positions the first conduit on the sample tank or the reagent tank, and then the elevating mechanism moves the first conduit to and away from each tank.

Next, the operation of the blood cell measuring apparatus of the present invention will be described. The sample liquid to be measured is blood. Then, for example, in the case of two-stage dilution of blood, saline is used for the first reagent and the second reagent. When a hemolytic agent is added to blood, it is added as a second reagent. A physiological saline solution is used as the working liquid of each liquid suction / ejection means, and the operation of the sample adjusting device constituting this blood measuring device is the same as that of the above-mentioned sample adjusting device, and therefore its explanation is omitted here.

After adjusting the blood, the sample adjusting device discharges the finally adjusted adjusted blood sample (referred to as blood sample) from the first conduit to the sample receiving part of the flow cell, and the detecting means is provided downstream from the sample receiving part. Measures blood cells in a blood sample. After that, the first conduit of the sample adjusting device is moved to the cleaning means by the driving means, and the outside of the first conduit is cleaned by the cleaning means. Further, each liquid suction / ejection means is operated to eject the cleaning liquid from the first conduit,
Rinse the adjusted blood, blood sample, etc. remaining in the device.

〔Example〕

 Embodiments of the present invention will be described below with reference to the drawings.

FIG. 1 is a block diagram of the sample adjusting device, and FIG. 2 is an operation time chart thereof. This device is a device for mixing and reacting n kinds of reagents and samples one after another.

In FIG. 1, a thin tube 1 as a first conduit is for aspirating and discharging a reagent and a sample. The thin tube 1
There is a branch pipe 2 as a branch portion at the upper end of the. Intermediate pipes 3-1 to 3-n as n second pipe lines extend from the branch pipe 2, and a mixing chamber 4 which is an expansion pipe portion in which a lower portion is wide at each end and is narrower toward an upper portion. -1 to 4-n are
It is provided. From the upper part of each mixing chamber 4-1 to 4-n, working fluid pipes 5-1 to 5-n as a third pipe line extend. The hydraulic fluid pipes 5-1 to 5-n are connected to injection cylinders 6-1 to 6-n, which are liquid suction / discharge means, respectively. Mixing chambers 4-1 to 4-1 are selected from the injection cylinders 6-1 to 6-n.
The hydraulic fluids 7-1 to 7-n which also serve as cleaning fluids are filled up to 4-n. In addition, n reagent containers 9-1 to 9-n are arranged near the thin tube 1, and n kinds of reagents 8-1 to 8-n are stored in each. Further, a sample container 10 is arranged, and a sample 11 is contained therein.

The sample adjusting device configured as described above operates as follows.

i) Reagent suction process First, the injection cylinder 6-1 is discharged to discharge the hydraulic fluid 7-1 from the mixing chamber through the intermediate pipe 3-1 and the branch pipe 2 through the thin pipe 1. Then, the injection cylinder 6-1 is caused to perform a suction operation to suck a fixed amount of air into the thin tube 1. Then, the thin tube 1 is dipped in the reagent 8-1, and the suction operation is performed by the injection cylinder 6-1.
Aspirate the reagent 8-1 until the mixing chamber 4-1 is filled.

Next, the injection cylinder 6-2 is discharged to move the working fluid 7-2 from the mixing chamber 4-2 through the intermediate pipe 3-2 and the branch pipe 2 into the thin pipe 1.
And the reagent 8-1 remaining in the thin tube 1 is washed away. Suction operation of the injection cylinder 6-2 to operate the hydraulic fluid 7 in the thin tube 1.
-2 is pulled back to the front of the branch pipe 2 (inside the intermediate pipe 3-2), during which a small amount of the injection cylinder 6-1 is suctioned, and the tip of the reagent 8-1 is slightly pulled into the intermediate pipe 3-1. In this way, since air is present between the tip of the reagent 8-1 and the branch pipe 2, the branch pipe 2 does not come into contact with other reagents. Further, since air layers are contained at both ends of the reagent 8-1, the reagent 8-1 can be quantified.

In this state, the second reagent is aspirated. The hydraulic fluid 7 that has ejected the injection cylinder 6-2 and has returned to the front of the branch pipe 2.
-2 is advanced to a predetermined position of the thin tube 1. Then dip the capillary 1 into the second reagent 8-2. The syringe 6-2 is suctioned to suck the reagent 8-2 until the mixing chamber 4-2 is filled. After that, the washing of the reagent 8-2 remaining in the thin tube 1 and the quantification of the reagent 8-2 are performed by the suction / ejection operation of the injection cylinder 6-2 and the injection cylinder 6-3 as described above. The third and subsequent reagent suctions are performed by repeating the same operation as in the second case.

ii) Sample suction process After the predetermined nth reagent suction is completed, the sample suction is started. In this example, the first reagent 8-1 is mixed and reacted in order. First, the syringe 6-
1 is discharged, and the reagent 8-1 which has returned to the front of the branch pipe 2 is advanced to the vicinity of the outlet of the thin pipe 1. In this state, move the capillary tube 1 to the sample container 10 and
11 Soak in. The injection cylinder 6-1 is suctioned to suck a fixed amount of the sample 11 into the thin tube 1. After that, the thin tube 1 is pulled up from the sample liquid surface. The syringe cylinder 6-1 is again made to perform a suction operation, and all the sucked sample 11 is introduced into the mixing chamber 4-1.

iii) Reagent addition process Sample 11 is introduced into mixing chamber 4-1 when mixing chamber 4-
Since the inlet of No. 1 has a sharply expanded shape, a vortex is generated near the inlet, and the sample 11 and the reagent 8-1 are mixed. If the mixing of the liquids is not sufficient after the first introduction, the ejection and suction operations of the injection cylinder 6-1 are performed several times, and the mixed liquid of the sample 11 and the reagent 8-1 is mixed with the intermediate pipe 3-1. Move back and forth between chambers 4-1 to complete mixing. Hereinafter, this mixed solution is referred to as a first adjustment sample.

When the mixing with the reagent 8-1 is completed, the process moves to a process of mixing a fixed amount of the first adjustment sample and a fixed amount of the reagent 8-2. First, the injection cylinder 6-1 is discharged to advance the first adjustment sample to the thin tube 1 via the branch tube 2. Simultaneously with the passage of the liquid through the branch pipe 2, the suction operation of the injection cylinder 6-2 is started, and a predetermined amount of the first adjusted sample is introduced into the mixing chamber 4-2 through the pipe 3-2. When that ends,
The injection cylinder 6-1 once performs a suction operation, returns the first adjustment sample remaining in the thin tube 1 to the position of the intermediate pipe 3-1, and the rear end of the adjustment sample toward the mixing chamber 4-2 is connected to the branch pipe 2 At that point, cut in the air layer to perform quantification. Mixing chamber 4
-2 also has the same shape as the mixing chamber 4-1, and the first adjustment sample and the reagent 8-2 can be mixed by the same method. Hereinafter, different reagents are mixed one after another, but the same procedure as above is followed.

FIG. 2 shows a time chart of the above operation, focusing on the suction / discharge operation of each syringe.

In the above process, first aspirate all reagents and then sample
Although 11 was sucked, the sample 11 may be sucked immediately after the first reagent 8-1 is sucked. While mixing the sample 11 and the reagent 8-1 in the mixing chamber 4-1, the reagent 8-
Aspirate 2 and make first preparation sample and reagent 8 there
When the reagent 8-3 is sucked while the -2 is mixed, the total sample preparation time can be significantly reduced as compared with the above-mentioned embodiment.

Further, the injection cylinders 6-1 to 6-n or the hydraulic fluid pipe 5-1.
If a predetermined reagent is placed in advance in ~ 5-n, the reagent suction process can be skipped, and in this case as well, it greatly contributes to shortening the overall adjustment time.

Next, an embodiment of the blood cell measuring apparatus of the present invention will be described with reference to FIGS. 3, 4, and 5. FIG. 3 is a detailed configuration diagram of the blood adjusting unit, FIG. 4 is an overall view of the blood cell measuring apparatus, and FIG. 5 is a time chart of the operation of the apparatus focusing on the movement of the syringe.

First, the configuration of the sample adjusting unit of this embodiment will be described with reference to FIG. The sample adjusting unit 12 is mounted below the tip of an arm 13 which can be independently rotated and moved up and down. Below the sample adjusting section 12, a thin tube 14 for inhaling blood, reagents, adjusted samples, etc. is attached downward. At the tip of the thin tube 14, a rubber spherical ring 34 is inserted to facilitate connection with the sample receiver 36 of the flow cell 35. The sample receiver 36 is provided with a suction port 37 for sucking the overflowed liquid. In the sample adjusting unit 12, three mixing chambers are built in. In FIG. 3, the center is a mixing chamber 20 for performing the first dilution, and the left is a mixing chamber 19 for performing the second dilution. The right is the mixing chamber 21 for hemolysis. Each mixing chamber has a triangular shape with an expanded lower part. A flow path 17 extends straight downward from the bottom of the mixing chamber 20, and is connected to the inner flow path of the narrow tube 14 via a branch portion 15 midway. Separate flow paths 16 and 18 extend from the branch portion 15 to the left and right, and bend upward in the middle to form mixing chambers 19 and 2, respectively.
It is connected to the bottom of 1. At branch portion 15, flow path 1
6, 18 are relatively thinner than the flow path 17. Hydraulic fluid channels 22, 23, 24 extend upward from positions corresponding to the tops of the mixing chambers 19, 20, 21, respectively. The flow paths are filled with physiological saline solutions 31, 32 and 33, respectively, as hydraulic fluid.

Next, the overall configuration of the blood cell counter will be described with reference to FIG. The hydraulic fluid flow paths 22, 23, 24 in FIG. 3 pass through the arm 13 and are connected to the injection cylinders 46, 45, 44, respectively. Each of the injection cylinders 46, 45, 44 can independently perform suction / ejection operation. A sample container 38, a hemolytic agent container 40, and a washing tank 42 flow cell 35 are arranged in this order from the foremost position under the trajectory of the arm 13. Blood 39 is contained in the sample container 38, and hemolytic agent 41 is contained in the hemolytic agent container 40. In the cleaning tank 42, the cleaning liquid is sprayed from the area surrounding the thin tube 14. The flow cell has an irradiation light source 47 for measurement and a detector for taking in signals from blood cells.
48 are provided.

The blood cell measuring device configured as described above operates as follows.

i) Washing process First, the arm 13 is swung and lowered to the washing tank 42 to wash the periphery of the thin tube 14. At the same time, the injection cylinders 44, 45, 46 are discharged together to flush the mixing chambers 19, 20, 21, the channels 16, 17, 18 and the channels in the narrow tube 14 with physiological saline.

ii) Inhalation process of physiological saline / hemolytic agent First, the mixing chambers 19 and 20 are filled with the physiological saline for dilution. The injection cylinders 45 and 46 are suctioned to inhale air. When the air is inhaled to the flow paths 16 and 17, the ejection operation of the injection cylinder 44 is started, and the working fluid 33 of the physiological saline is sent to the flow paths 16 and 17. When this physiological saline solution fills the mixing chambers 19 and 20 by a certain amount, the operation of the syringe barrel 44 is stopped, and after a while, the syringe barrel 4 is stopped.
Stop the operation of 5,46. By doing so, air enters the portions of the flow paths 16 and 17 that are connected to the branch portion 15. In this state, the flow path inside the thin tube 14 is filled with air.

Next, the hemolyzing agent suction operation is started. The arm 13 is swung to descend into the hemolytic agent container 40, and the thin tube 14 is immersed in the hemolytic agent 14. The injection cylinder 44 is suctioned, and the hemolytic agent 41 is sucked until the mixing chamber 21 is filled with a certain amount. Finally, a small amount of air is sucked into the flow path inside the narrow tube 14.

iii) Sample inhalation process In the above state, the hemolytic agent is filled from the flow passage in the narrow tube 14 to the branch portion 15 and the flow passage 18. In addition, the flow path 1 from the branch portion 15
There is air up to 6 and 17 and the rest is filled with saline.

The blood in the sample container 38
Immerse the thin tube 14 inside. The injection cylinder 44 is suctioned to suck a certain amount of blood from the flow path in the thin tube 14 into the flow path 18 and stop. Next, the suction operation of the injection cylinder 45 is started to supply a certain amount of blood 39 to the flow path.
Inhale to 17 and stop. Raise the arm 13 to move the thin tube 14
Away from the surface of blood 39. Again, the injection cylinder 45 is caused to perform a suction operation, and the blood 39 filling the flow path inside the thin tube 14 and the flow path 17 is sucked into the mixing chamber 20. At the time of the above-mentioned inhalation, as soon as the blood 39 passes through the branch portion 15, the injection cylinder 44 is caused to perform an aspiration operation to inhale the blood 39 in the flow path 18 into the mixing chamber 21.

At this time, in the above operation, both physiological saline, hemolyzing agent, and blood are inhaled after being segmented with air at both ends, so that quantitativeness such as a blood sampling amount and a dilution ratio is not impaired.

iv) Mixing (dilution / hemolysis) process When the blood 39 is introduced into the mixing chambers 20 and 21, a rapid expansion inflow from the flows 17 and 18 promotes agitation. Further, the injection cylinders 44 and 45 are alternately repeated several times for suction operation and discharge operation to cause the liquid to reciprocate between the mixing chamber and the middle of the flow paths 17 and 18. This completes hemolysis in the mixing chamber 21 and produces a blood sample containing only white blood cells. Further, in the mixing chamber 20, the first-stage blood diluted sample is completed. Next, the syringe 45 is discharged to advance the first-stage diluted blood sample from the branch portion 15 to the flow path in the narrow tube 14, and at the same time, the syringe 46 is suctioned to suck a fixed amount of the first-stage diluted blood sample into the mixing chamber 19. To do.
Suction the syringe barrel 45 to pass the one-step diluted blood sample
After returning to 17, the rear end of the single-diluted blood sample sent to the mixing chamber 19 is cut off with air. Then, the syringe 46 is suctioned to introduce the first-stage diluted blood sample into the mixing chamber 19 and mix it uniformly.

v) Measurement process The arm 13 is swung to move above the sample receiver 36 of the flow cell 35. In this state, the injection cylinder 46 is discharged,
The two-stage diluted blood sample in the mixing chamber 19 is advanced to the tip of the channel in the thin tube 14. Next, lower the arm 13 to make a spherical ring.
Connect 34 and sample receiver 36. The injection cylinder 46 is slowly discharged, and the two-stage diluted blood sample is sent to the measurement point. When this measurement is completed, raise the arm 13 slightly,
The spherical ring 34 and the sample receiver 36 are separated. Next syringe
The discharge operation of 44 is started, the two-stage diluted blood sample in the thin tube 14 is expelled, and the tip is filled with the hemolyzed sample. The expelled double-diluted blood sample is dripped at the sample receiver 36 and is inhaled through the inhalation port 37. Once again, the arm 13 is lowered to connect the thin tube 14 to the flow cell 35, and the injection tube 44
To discharge the hemolyzed sample to the measurement point.

vi) Washing process When the measurement is completed, the arm 13 is moved to the washing tank 42. The outside of the thin tube 14 is washed with washing water sprayed from inside the washing tank 42. In addition, clean the sample adjustment unit 12 with each injection cylinder.
44, 45, 46 are simultaneously discharged, and the respective working fluids 31, 32, 33 are made to flow from the mixing chamber to the respective flow passages for washing. The portion through which the blood sample passes is limited to the inside of the sample adjustment unit 12, and since there is no valve or the like where the sample easily stays, it is possible to wash in a short time with a small amount of hydraulic fluid.

The blood cell measuring apparatus described above is an example in which one sample adjusting unit is provided, but a plurality of sample adjusting units may be provided.

If a plurality of samples are provided and the samples are adjusted one after another at the same time, the throughput (processing amount) can be significantly improved. Since the structure of the sample adjustment part is simple, it is relatively easy,
Plural can be provided.

Further, the sample adjusting unit of the present embodiment has no valve body like the above-described sample adjusting device, and the portion through which blood passes is overwhelmingly short as compared with the conventional one, so that the sample adjusting unit depends on the protein component in blood. Almost no dirt or blood cell retention or adhesion. Therefore, cross-contamination with the next sample to be measured and carryover of blood cells, which causes carryover error, can be significantly suppressed.

〔The invention's effect〕

According to the present invention, the sample adjusting device includes a first pipe line for sucking / discharging a sample liquid and various reagents, and a plurality of expansion pipe parts and liquids respectively connected to the first pipe line via a branch part. Since the flow path group is composed of the suction / ejection means, the structure can be simple, and thus the cost of the device can be significantly reduced as compared with the conventional device. Further, the operation of adding the reagent to the sample liquid to adjust the sample liquid is performed by moving the sample liquid and the reagent between the expansion pipe section and the first pipe line by the liquid suction / ejection means and processing them outside the apparatus. Is clean,
It is possible to perform adjustment with high processing speed and high accuracy.

Further, according to the present invention, since the blood cell adjusting apparatus is configured by combining the above sample adjusting apparatus and the flow cell for measuring blood cells, there is no valve body, and the flow path through which blood passes is overwhelmingly compared with the conventional one. Since it is very short, it is possible to significantly reduce stains by blood and adhesion and retention of blood cells. Therefore, mutual contamination between samples and carryover can be suppressed, and as a result, measurement accuracy can be improved.

[Brief description of drawings]

FIG. 1 is a block diagram of the sample preparation device, FIG. 2 is an operation time chart of the sample preparation device, FIG. 3 is a sectional view of the blood cell measurement device of the present invention, FIG. 4 is an overall block diagram of the blood cell measurement device, FIG. 5 is an operation time chart of the blood cell measuring apparatus. 1,14 ... Thin pipe (first pipe line), 2 ... Branching pipe, 3-1 to 3-n ... Intermediate pipe (second pipe line), 4-1 to 4-n, 19 to 21 ... Mixing chamber (expansion pipe part), 5-1 to 5-n ... Hydraulic fluid pipe (third pipe line), 6-1 to 6-n, 44 to 46 ... Injection cylinder (liquid suction / discharge means), 9-1 to 9-n ... Reagent container, 10,38 ... Sample container, 12 ... Sample adjusting unit, 13 ... Arm, 15 ... Branching unit, 35 ... Flow cell, 36 ... Sample receiving unit, 42 …… Cleaning tank, 48 …… Detector.

Claims (4)

[Claims] 1. A sample adjusting device for adding various liquid reagents to a sample liquid to be analyzed to adjust the sample liquid, and a first conduit having pores for sucking the sample liquid and the various reagents at one end. A branch portion provided at the other end of the first pipeline, a plurality of second pipelines extending by connecting one end to the branch portion, and an expansion tube provided at the other end of each of the second pipelines A passage, a third pipe extending at one end thereof to a reduction port provided in each of the expansion pipes, and a liquid suction / ejection means connected to the other end of the third pipe. Characteristic sample preparation device. 2. The sample preparation device according to claim 1, a sample tank for storing the sample liquid, a reagent tank for storing the liquid reagent, and a pore of the first conduit immersed in the sample tank and the reagent tank. And a driving unit for driving the sample adjusting device. 3. The sample adjusting device according to claim 2, wherein the driving means comprises a turning mechanism for turning the first conduit and an elevating mechanism for moving up and down. 4. A first conduit having pores for sucking blood and various liquid reagents added to the blood at one end, a branch provided at the other end of the first conduit, and the branch. A plurality of second pipelines that are connected to one end to extend, expansion tubes provided at the other ends of the second pipelines, and one ends connected to reduction ports provided at the expansion tubes. A sample adjusting device including a third extending pipe and liquid suction and discharge means connected to the other end of the third pipe, a blood tank for storing the blood, and a reagent tank for storing the liquid reagent, respectively. A cleaning means for cleaning the outside of the first conduit, a sample receiving part for receiving a prepared blood sample composed of the blood and the liquid reagent mixed by the sample preparation device from the first conduit, and a sample receiving part of the sample receiving part In the blood sample prepared downstream A flow cell comprising a detecting means for detecting the blood cells, the blood vessel the first conduit, reagent reservoir,
A blood cell measuring device comprising: a cleaning means and a drive means for moving the sample to the sample receiving portion.