JPH0436636Y2 - - Google Patents

Description

[Detailed Description of the Invention] (Industrial Application Field) The present invention relates to a cell culture cell used in a method of transferring DNA, etc. into living cells by irradiating living cells with a laser beam under a microscope.

(Prior Art) The present inventors previously filed a patent application for a device for transferring DNA, etc. into living cells by irradiating them with a laser beam (Japanese Patent Application No. 191378-1982).

In this case, the living cells in the culture cell are irradiated with a laser beam, but the living cells must be placed within the focal plane of the laser beam, and in order to irradiate the laser while the cells are alive, they must be immersed in the culture medium. It is necessary that the However, since the culture solution has low transmittance to ultraviolet light, the cells are attached to a cover glass such as a quartz plate and a laser is irradiated through the plate. The culture cell used in this case has the configuration shown in FIG. 4, which is a longitudinal cross-sectional view, and FIG. 5, which is a cross-sectional view thereof. As shown in the figure, this cell culture cell 11 is provided with an opening 13 in addition to a culture solution inlet 12 for injecting a culture solution into the cell, and living cells 14 are planted in advance in this opening 13. A cover glass 15 is provided via a rubber packing 16. In use, the culture cell 11 is raised, the culture solution 17 is filled into the cell 11 through the culture solution inlet 12, and the culture solution inlet 2 is plugged with a stopper 18. This is placed under a microscope 19 as shown in the figure, and a laser beam 20 focused on the culture cell 1 by the microscope 19 is irradiated onto the cover glass 15, that is, the living cells.

(Problem that the invention aims to solve) However, in this cell culture cell, the cells are planted on a cover glass, but in the case of bacterial cells, plant cells, etc., the cells do not attach to the cover glass and are not placed in the culture medium. Because it floats and does not settle,
It is difficult to place living cells within the focal plane of the laser beam. The purpose of the present invention is to provide a cell culture cell for laser irradiation that is suitable for arranging living cells such as bacterial cells and plant cells that float in a culture medium and do not precipitate within the focal plane of a laser beam. .

Furthermore, another object of the present invention is to provide a cell culture cell for laser irradiation that can efficiently irradiate a large number of living cells with a laser beam. In other words, in the past, when irradiating living cells with a laser, it took time to target each living cell one by one, and if the laser beam was scanned at a constant speed and at regular intervals, there were many cases where the laser beam did not hit the living cells. The present invention attempts to solve these problems as well.

(Means for Solving the Problem) The above-mentioned problem is caused by cell culture in which a convex section is provided around which the culture solution tank is formed, and a groove is formed on the top surface of the convex section for the cells to enter. The solution is to use cells.

(Functions and Effects) Live cells fit into the grooves provided on the upper surface of the convex part, and the living cells are regularly arranged in the grooves, so the living cells do not float in the culture medium and are exposed to the laser beam. If the laser beam is placed in the focal plane and scanned along the array of living cells, a large number of living cells can be efficiently irradiated with the laser beam.

(Example) Hereinafter, the cell culture cell of the present invention will be explained in detail using the drawings. FIG. 1 is a sectional view of an embodiment of a cell culture cell according to the present invention, FIG. 2 is a partially enlarged view thereof, and FIG. 3 is a partially enlarged perspective view of the same embodiment.

The cell culture cell 1 according to the present invention has a convex section 2, and a culture solution tank 3 is formed around the convex section 2. On the upper surface of the convex portion 2,
A plurality of grooves 4 having a depth (H=0.01 to 0.05 mm) approximately equal to the diameter of the cell are formed. Therefore, as shown in FIG. 1, after pouring the culture solution 5 into the culture solution tank 3, the culture solution containing living cells 6 is dripped onto the upper surface of the convex portion 2, and then a cover glass 7 is placed. Place it on cell culture cell 1. The living cell 6 enters the groove 4 and
A large number of living cells are arranged along the line (see Figure 2). Living cells 6 are moistened with culture solution 5. When such a state is achieved, it becomes possible to place a large number of living cells within the focal plane of the focused laser beam 9. Scanning of the laser beam 9 is performed along the array of living cells, and a large number of living cells are efficiently irradiated with the laser beam.

[Brief explanation of the drawing]

1, 2, and 3 are a sectional view, a partially enlarged sectional view, and a partially enlarged perspective view of an embodiment of the cell culture cell of the present invention, and FIG. 4 is a conventional cell culture cell. FIG. 5 is a vertical cross-sectional view of the fourth
It is a sectional view taken along the line VV in the figure. 1, 11... Cell culture cell, 2... Convex section, 3... Culture solution tank, 5, 17... Culture solution,
6,16...Living cells, 7,15...Cover glass, 9,20...Laser beam.

Claims (1)

[Scope of utility model registration request] A cell culture cell that is provided with a convex cross-sectional portion whose periphery serves as a culture solution tank, and a groove into which the cells enter is formed on the upper surface of the convex cross-sectional portion.