JPH0517816B2 - - Google Patents

Description

Detailed Description of the Invention (Industrial Field of Application) The present invention is directed to growing cultured seedlings obtained by plant tissue culture technology as seedlings for planting, and also for growing cultured seedlings obtained by plant tissue culture technology in a growing environment for seedlings for planting. This invention relates to an acclimatization method and device for gradually acclimating children to the environment.

(Prior art and its problems) The conventional method of producing cultured seedlings using plant tissue culture technology is to first culture the cultured seedlings in an agar medium in an incubator such as an Erlenmeyer flask under sterile conditions. ,
The plants are taken out of the culture vessel, the agar used as a medium is washed off, and then transplanted to a hydroponic or potted planting medium.

However, cultured seedlings obtained by tissue culture technology have weak resistance to the environment, so in the conventional method described above, they tend to exhibit growth suppression or wither after being removed from the culture vessel and transplanted, resulting in However, the conventional yield rate was as low as 20 to 50%, and even if it were to grow, it would take a long time. These causes are considered to be internal causes and external causes as follows.

First of all, the internal cause is the high humidity in the culture vessel when the cultured seedlings
Because they grow in a low-light environment, they have insufficient root development and the ability to regulate the opening and closing of stomata. It's low.

Next, external factors include planting pain due to root breakage when the cultured seedlings are taken out of the culture vessel and transplanted into an acclimatization greenhouse, etc., and rapid changes in medium components and water potential at the time of transplantation.

The above-mentioned conventional methods do not deal with these internal and external causes, and therefore, as mentioned above, after transplantation, the plants tend to exhibit growth restriction or die.

The present invention aims to solve the above-mentioned conventional problems by effectively eliminating these causes.

(Means for Solving the Problems) The structure of the present invention will be explained based on the drawings corresponding to the examples. Granules 1
Cultured seedlings 4 tissue-cultured in a medium 3 consisting of agar 2 mixed with agar 2 are transplanted together with the medium 3 into a support container 7 configured in a seedbed 6 of a hydroponic cultivation device 5.

Next, in the method described in item 2, in the method described in item 1, the support container 7 is configured as a net-like body with a mesh smaller than the particle diameter of the granular material 1.

Next, in the method described in item 3, in the method described in item 1, the support container 7 includes a plurality of mesh bodies 7a, 7b whose meshes are gradually enlarged from the inside to the outside.
7c are stacked on top of each other, and the inner net-like body 7
a, 7b are constructed so as to be separated and removable, and the net-like bodies 7a, 7b are removed from the inside, and the granules 1b, 1c of which the particle size has been gradually increased are supplied to adjust the particle size of the granules 1 in the support container 7. is gradually increased.

Next, in the method described in item 4, in the method described in item 1, the hydroponic cultivation device 5 is configured to be able to adjust the water level of the hydroponic liquid 8, and by adjusting the water level of the hydroponic liquid 8. This is to control the moisture content of the medium 9 made of the granules 1.

Next, in the method described in item 5, in the method described in item 1, the granules 1 have rounded corners.

Next, the apparatus described in item 6 includes a tissue culture device 10 in which a culture medium 3 is formed by mixing granules 1 of a water-insoluble substance with good water absorption into agar, and a support container 7 for cultured seedlings 4 in a seedbed 6. The hydroponic cultivation apparatus 5 shown in FIG.

Next, in the apparatus described in item 7, in the apparatus described in item 6, the support container 7 is configured as a net-like body with a mesh smaller than the particle diameter of the granular material 1.

Next, in the apparatus described in item 8, in the apparatus described in item 6, the support container 7 includes a plurality of mesh bodies 7a, 7b whose meshes are gradually enlarged from the inside to the outside.
7c are stacked on top of each other, and the inner net-like body 7
a, 7b are constructed so as to be separated and removable, and the net-like bodies 7a, 7b are removed from the inside, and the granules 1b, 1c of which the particle size has been gradually increased are supplied to adjust the particle size of the granules 1 in the support container 7. is gradually increased.

Next, in the apparatus described in Item 9, in the apparatus described in Item 6, the hydroponic cultivation apparatus 5 is configured to be able to adjust the water level of the hydroponic liquid 8, and by adjusting the water level of the hydroponic liquid 8. This is to control the moisture content of the medium 9 made of the granules 1.

Next, the apparatus described in item 10 is the apparatus described in item 6, except that the granules 1 have rounded corners.

(Actions and Examples) Next, the operation of the present invention will be explained with reference to examples.

FIG. 1 is a conceptual diagram schematically showing the structure of the present invention, and in this diagram, the reference numeral 10 indicates a plant tissue culture device such as an Erlenmeyer flask, and the reference numeral 5 indicates a hydroponic cultivation device. In the present invention, incubator 10
As shown in FIG. 1, the culture medium 3 in the interior is composed of conventional agar 2 mixed with an appropriate amount of granular material 1 of a water-insoluble substance with good water absorption, and cultured seedlings 4 are grown in this medium 3. make it grow Note that, prior to mixing the granules 1 into the agar 2, they are sterilized by an appropriate method such as heat sterilization.

In the above medium, the cultured seedlings 4 grow while their roots are intertwined with the granules 1. And cultured seedlings 4
When it grows to a predetermined stage, it is transferred to the incubator 10.
The seedlings are taken out and transplanted into a support container 7 configured in a seedbed 6 of a hydroponic cultivation device 5, and then cultivated using a hydroponic solution 8.

In the present invention, since such transplantation is performed together with medium 3, agar 2 also remains immediately after transplantation. Then, as time passes, the agar 2 is gradually washed away by the hydroponic solution 8 or gradually dissolved in the hydroponic solution 8, and is finally completely removed, resulting in a medium 9 containing only the granules 1. Become. Incidentally, an appropriate amount of the granular material 1 is added as necessary over time.

When cultured in the incubator 10, the cultured seedlings 4 are accustomed to a high moisture content state, so when transplanting, as in the past, the medium 3 that was cultivated in the incubator 10 is washed off and the seedlings are rapidly When transplanted into a medium with a different moisture environment and medium composition, cultured seedlings that are susceptible to water stress and sudden changes in medium composition tend to exhibit growth suppression or die. Since agar 2 remains for a while, changes in the moisture environment and medium components will be gradual. Also, cleaning the culture medium,
The occurrence of root breakage during transplanting work can be prevented. Agar 2 is completely removed after a certain period of time, and from then on, only granular material 1 is used for cultivation.
It is also possible to prevent a large amount of mold and bacteria from forming due to Agar 2. It should be noted that the agar that has flowed or eluted into the hydroponic solution 8 can be easily configured to be reused as fertilizer by appropriate microbial treatment.

In the above method or device, the support container 7
The structure may be any suitable structure as long as the hydroponic liquid 8 can be circulated well, and for example, it can be easily formed by a net-like structure. In this case, the mesh of the net-like body is the granular body 1
The granular material 1 is held at a particle size smaller than that of the granular material 1.

In the above method or device, the support container 7
is constructed by stacking a plurality of mesh bodies 7a, 7b, 7c whose meshes are gradually enlarged from the inside to the outside, and the inner mesh bodies 7a, 7b are configured to be divided and removable, and the mesh body 7a , 7b is removed from the inside and the particle size is gradually increased.
b, 1c can be supplied to gradually increase the particle size of the granules 1 in the support container 7, and by gradually increasing the particle size of the granules 1 according to the growth of the plant, the size of the plants after cultivation can be increased. The roots and granules can be easily separated at the time of sale. Such a configuration is
For example, it is suitable for plants whose roots are used for medicinal purposes, ie, whose roots are commercially available. FIG. 2 schematically shows the above structure and operation.

First, FIG. 2a shows a diagram from the incubator 10 to the support container 7.
In this state, the support container 7 has the innermost minimum mesh size (for example, 3 mm).
A first reticular body 7a that is divisible (weak), a second reticular body 7b that is divisible and has an intermediate mesh located in the middle (for example, just under 5 mm), and an undividable one that has the outermost maximum mesh (for example, just under 9 mm). and the third net-like body 7c are all overlapped. The granules 1a are filled with particles having a diameter of 3 mm.

In this state, first, the first net-like body 7a is divided and removed upwardly, and the granules 1b having a diameter of 5 mm are added from above. In this way, as shown in FIG.
passes through the second net-like body 7b and the third net-like body 7c and falls downward, so as time passes, the granules 7b with a diameter of almost 5 mm become filled. Next, in the same manner as described above, first, the second net-like body 7b is divided and removed upwardly, and the granular body 1c having a diameter of 9 mm is added from above. In this way, as shown in FIG. 2c, the granules 1b with a diameter of 5 mm that are not entangled with the roots pass through the third net-like body 7c and fall downward.
With the passage of time, the granules 1c having a diameter of almost 9 mm can be filled.

In this way, by gradually increasing the particle size of the granular material 1 according to the growth of the plant, it is possible to easily separate the roots and the granular material 1 when selling the cultivated plant. If the particle shape of the particles 1 is rounded, such as a spherical shape or a spheroidal shape, separation can be further facilitated.

Next, in the above method or apparatus, if the hydroponic cultivation apparatus 5 is configured to be able to adjust the water level of the hydroponic liquid 8, the medium 9 made of the granules 1 can be adjusted by adjusting the water level of the hydroponic liquid 8. moisture content can be controlled.

That is, immediately after transplantation, the water level is maximized and the culture media 9 and 3 have a high moisture content, so that a moisture environment can be maintained. Next, after a certain amount of time has passed since transplantation, the water level can be lowered to lower the moisture content of the culture medium 9, and in this way, cultivation can be performed at an appropriate moisture content depending on the type of plant. In addition to the above water content control, the water level was repeatedly raised and lowered to
As shown in the figure, at the lower water level _l1 , by exposing the support container 7 to the outside air,
It is possible to actively supply oxygen to the roots.

In the present invention, although the granular material 1 is a water-insoluble substance, it has good water absorption properties, so it can prevent the roots of plants from drying out, and in this case, the growth of the roots is suppressed and the roots die. It can be prevented.

As long as the water-insoluble substance has good water absorption and can be formed into the granular material 1, an appropriate material such as a ceramic filler such as diatomaceous earth, activated carbon, wood chips, charcoal chips, or urethane can be applied. can do. Further, the particle size of the granular material 1 can be appropriately set depending on the type of plant, its use, etc., such as the particle size in the example described above.

(Effects of the Invention) As described above, the present invention comprises a seed bed of a hydroponic cultivation device in which cultured seedlings are tissue-cultured in a medium containing agar mixed with granules of a water-insoluble substance having good water absorption properties. Since the seedlings are transplanted into a support container together with the medium, the above-mentioned internal causes may occur, namely, the cultured seedlings grow in the culture container under a high humidity and low light environment, resulting in insufficient root development and the ability to regulate the opening and closing of stomata. At the same time, it compensates for the small number of cuticle waxes, that is, their vulnerability to water stress in general, and their low chlorophyll content and low photosynthetic ability, and also compensates for external factors, namely, removing the cultured seedlings from the culture vessel and acclimating them in an acclimatization greenhouse. Effectively prevents growth suppression and death by eliminating planting pain caused by root breakage, etc. when transplanting indoors, etc., and sudden changes in medium components and water potential at the time of transplantation. This has the special effect of improving the yield in the production of cultured seedlings using tissue culture technology.

[Brief explanation of the drawing]

Figures 1 a, b, c, d, and e are conceptual diagrams schematically showing the structure of the present invention over time;
Figures a, b, and c are conceptual diagrams schematically showing the structure of the support container in the present invention over time. Code, 1, 1a, 1b, 1c...granular material, 2...
... Agar, 3 ... Culture medium, 4 ... Cultured seedlings, 5 ... Hydroponic cultivation equipment, 6 ... Seedbed, 7 ... Support container, 8 ...
Hydroponic solution, 9... Culture medium, 10... Tissue culture device, 7
a, 7b, 7c...reticular body.

Claims (1)

[Scope of Claims] 1. Cultured seedlings that have been tissue cultured in a medium containing agar mixed with granules of a water-insoluble substance having good water absorption are placed in a support container configured as a seedbed of a hydroponic cultivation device, A method for acclimating cultured seedlings, which comprises transplanting them together with the medium. 2. The method for acclimatizing cultured seedlings according to item 1, wherein the support container is configured as a net-like body with a mesh smaller than the particle size of the granules. 3 In the method described in paragraph 1, the support container is constructed by stacking a plurality of mesh bodies whose meshes gradually increase in size from the inside to the outside, and the inner mesh body is constructed so that it can be divided and removed. . A method for acclimating cultured seedlings, which comprises removing the mesh from the inside and supplying granules whose particle size is gradually increased to gradually increase the particle size of the granules in the support container. 4. In the method described in paragraph 1, the hydroponic cultivation device is configured to be able to adjust the water level of the hydroponic solution, and the water content of the medium made of granules is controlled by adjusting the water level of the hydroponic solution. A method for acclimating cultured seedlings, characterized by: 5. A method for acclimating cultured seedlings according to the method described in item 1, characterized in that the granules have rounded corners. 6. It is characterized by being composed of a tissue culture device whose medium is made up of agar mixed with granules of a water-insoluble substance having good water absorption properties, and a hydroponic cultivation device whose seed bed is a support container for cultured seedlings. Acclimatization device for cultured seedlings. 7. The apparatus for acclimatizing cultured seedlings according to item 6, characterized in that the support container is configured as a net-like body with a mesh smaller than the particle size of the granules. 8 In the device described in item 6, the support container is composed of a plurality of overlapping mesh bodies whose meshes are gradually enlarged from the inside to the outside, and the inner mesh body is constructed so that it can be divided and removed. An acclimatization device for cultured seedlings, which is characterized by: 9. The apparatus for acclimatizing cultured seedlings according to item 6, wherein the hydroponic cultivation apparatus is configured to be able to adjust the water level of the hydroponic solution. 10. The apparatus for acclimating cultured seedlings according to item 6, wherein the granules have rounded corners.