JPH052016A - Urine test container - Google Patents

Abstract

PURPOSE:To obtain a urinalysis container which is not necessary to be prepared for every use and elongates the usable term of a urinalysis reagent, and is small in volume when stored. CONSTITUTION:A urinalysis container 1 is formed using a gas barrier sheet. An opening of the container is sealed thereby to seal the inside of the container. Moreover, a composition including a urinalysis reagent is applied in the container 1 to define a urinalysis reagent section 4.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an improved urine test container in which it is not necessary to prepare a container each time it is used, the urine test reagent can be used for a long period, and the volume during storage is small. It is a thing.

[0002]

2. Description of the Related Art Conventionally, for the purpose of detecting sugar, protein, pH, occult blood, etc. in urine, a urine test paper in which a filter paper is impregnated with a urine test reagent or a urine test stick using a plastic stick is used. It is being used.

[0003]

However, in this method, there is an inconvenience that the urine collection container and the urine test paper and the like must be prepared separately, and in general, several tens of urine test paper and the like are collectively packaged. Since it is hermetically sealed, there is a disadvantage that the inspection function is deteriorated by the action of moisture and carbon dioxide gas in the air once opened.

The inconvenience of separately preparing the container and the test paper etc. is solved by the urine test paper cup in which the test paper is attached to the inner surface of the paper cup or the like, but since the test paper is exposed, it can be used. However, if the paper cup openings are closed with a lid, stacking will not work, and even if several paper cups are stacked and sealed, they will still be bulky.

The present invention solves the above-mentioned drawbacks of the prior art, does not require the trouble of separately preparing a test sheet and a container for a urine test, and the usable period of the reagent in the urine test reagent area. It is an object of the present invention to provide a urine test container that has a long length, has a small volume, and does not take up much space during storage.

[0006]

The urine test container of the present invention has a urine test reagent area formed by applying a composition containing a urine test reagent to the inner surface of the container formed of a gas barrier sheet. It is characterized in that the inside of the container is sealed by being formed and sealing the opening of the container.

[0007]

[Function] A urine test container is constructed by using a gas barrier sheet, the opening is sealed to seal the inside of the container, and a composition containing a urine test reagent is applied to the container for urine test. The reagent area is formed so that the urine test reagent can be used for a long period of time, and the urine test can be performed very easily.

[0008]

The present invention will be described in detail below with reference to the drawings. FIG. 1 shows one of the embodiments of the present invention, in which a container 1 is a packaging bag made of a gas barrier sheet,
The inside is sealed by sealing the hatched portion in the drawing, and in order to show the inside, in the drawing, a part of which is removed by the cutout line 2 is provided on the inner surface of the packaging bag of the backside sheet 3 to be described later. By applying the composition containing the urine test reagent as described above, the urine test reagent area 4 is formed. As described above, in the first embodiment of the present invention, the urine test reagent area is formed on the inner surface of the hermetically sealed packaging bag configured by using the gas barrier sheet. In such an embodiment, as shown by 5 in FIG. 1, a V-shaped cut 5 is provided to facilitate opening by tearing, or a cut cut by about half the thickness of the sheet is perforated. It can be opened without using any tools. In either method, the urine to be inspected is sampled by opening the bag, the urine test reagent area 4 is brought into contact with the urine for coloration, and comparison is made with a standard color prepared in advance to make a determination.

Since the shape shown in FIG. 1 cannot stand up by itself, a container is constructed by using a gas barrier sheet having a large thickness and a relatively large rigidity, and it is erected in an appropriate auxiliary tool or partition box. Alternatively, it is preferable to provide a re-sealing means such that a plastic chuck is provided in the vicinity of the opening after opening and the collected urine does not flow out to the outside even if it collapses. In addition, since the one shown in FIG. 1 is difficult to hold at the time of collecting urine, the vicinity of the gas barrier sheet which becomes the opening after opening is preliminarily thickened to partially increase the rigidity, or a separate sheet is used for the container. When reinforced from the inside or the outside, it can be opened by pushing both sides of the opening and is easy to hold. Further, the hand-held portion may be embossed to make it less slippery.

FIG. 2 shows another example of the urine test container of the present invention, and is a plan view with a part of the front side cut away to show the inside thereof. The container 6 in FIG. 2 represents a self-supporting packaging bag,
It is composed of a front side sheet 7 made of a gas barrier sheet, a back side sheet 8 and each sheet of a bottom sheet 9 which is inserted between both sheets and whose fold lines are shown by broken lines. It is sealed. A urine test reagent zone 4 is formed above the inside of the back sheet 8 by applying a composition containing a urine test reagent as described later. As shown in a sectional view taken along the line AA of FIG. 3, the packaging bag 6 has a front side sheet 7 and a back side sheet 8 in most of the side seal portions without the bottom sheet 9. It is directly sealed, and in a part of the bottom sheet 9, the space between the front side sheet 7 and the bottom sheet 9, the folded insides of the bottom sheet 9 and the respective portions between the bottom sheet 9 and the back side sheet 8 are sealed. There is. Further, the packaging bag 6 has a cross-sectional view taken along the line BB as shown in FIG.
In the portion other than the side seal, the front side sheet 7 and the back side sheet 8 are directly sealed at the upper edge portion 10, and the lower edge portion 11
In the above, both positions are sealed between the front side sheet 7 and the bottom sheet 9 facing each other, and between the back side sheet 8 and the bottom sheet 9 facing each other. There is no sealing between the folded inner sides of the bottom sheet 9, the majority of the upper portion 12 of the bottom sheet 9 and both the front and back sheets facing that portion. The packaging bag 6 having such a structure can be self-supporting because the lower end 13 becomes a thread bottom having a shape close to a circle by injecting a liquid, blowing a breath, or spreading it by hand ( 5), it is more suitable to stand upright on a horizontal place after collecting urine than that of FIG. Further, the container shown in FIGS. 2 to 5 may be provided with a means for opening the container shown in FIG. 1 and an additional means for producing an advantage in use. As described above, in the second embodiment of the present invention, the urine test reagent area is formed on the inner surface of the hermetically sealed packaging bag formed by using the gas barrier sheet, and the packaging bag is opened to open the bottom portion. By expanding it, it stands up by itself without any auxiliary means, that is, it is self-supporting.

FIG. 6 shows a third example of the urine test container of the present invention, in which the urine test reagent zone 4 is formed by applying a composition containing a urine test reagent inside the container 14. There is something.

As will be described later, the container 14 has an outer surface made of, for example, paperboard and an inner surface made of a gas barrier sheet, and has perforated cuts 15, 16, 17, and 18 that do not reach the inside. The front side material 19 is pulled toward the front of the drawing and the back side material 20 is pulled toward the back of the drawing after opening the upper portion, or preferably, the left and right side edges 21 and 22 are directed toward the center of the container. By pushing, the whole container can be expanded, and the shape as shown in FIG. FIG. 8 shows a state in which the shape shown in FIG. 7 is turned over.

FIG. 9 shows a development view of the material for making the container 14 shown in FIGS. 6 to 8. The surface is a material having high rigidity such as paperboard 23, and the inner surface is gas barrier property. It consists of a seat 24. The paperboard 23 and the sheet 24 are laminated by heat fusion or the like on a necessary portion, preferably on the entire surface of the paperboard 23. Here, the size of the sheet 24 may be the same as that of the paper board 23, and is drawn larger for convenience of showing the shape of the sheet 24. Also, four folded pieces 2
Although the tip ends 29 and 30 and 31 and 32 of 5 to 28 are in contact with each other, they are also drawn with a gap for convenience in order to clearly show the shape and the folding line 35 of the sheet 24.
Moreover, in the material shown in FIG.
Perforations 15, 15 ', 16, 16', 1 which reach most of the thickness of the board, preferably the total thickness of the paperboard
7, 17 ', 18, 18', 33, 33 ', 34 are provided. The material of the development view of FIG. 9 is bent inward along the perforations 34 and the fold lines 35, and the perforations 33,
The inside is sealed by bending outward along 33 'and sealing the left and right sides and the three sides, and the shape shown in FIG. 6 can be obtained. Also, the container 1 shown in FIGS.
The upper part of 4 may be opened with scissors or the like, but a tool for opening may be unnecessary by providing a cut line 38 for opening.

The third embodiment of the present invention whose embodiment is shown in FIGS.
In the embodiment of (1), since the packaging bag made of a flexible sheet is reinforced with a rigid material such as a hard paperboard, the packaging bag will return to its original shape and fall down with a small external force once opened and once expanded. It is easy to maintain its shape when held by hand. In this embodiment, in addition to the embodiment shown in FIGS. 6 to 9, folded pieces and 37 shown by 25, 26, 27 and 28 in FIG.
It is also possible to omit either or both of the paperboards at the bottom position indicated by. Also, perforations 17, 17 ', 1
Forming 8 and 18 'so that they reach the opening and bending them to the edge of the opening makes it easier to maintain the shape.
This is particularly effective when the folded pieces 5 to 28 and the bottom portion 37 are omitted. Alternatively, perforations 15, 15 ', 16, 16' may be used as folding lines, or perforations 17, 17 ', 18, 18'
Can be omitted, and in this way the shape of the container can be simplified.

In each of FIGS. 1 to 9 and subsequent FIGS. 10 to 12, the manufacturer's name, application, date of manufacture, expiration date, usage, precautions, etc. are printed so that they can be seen from the outside of the container. You can do it.

In the above, the containers 1 and 6 and the container 1
As the sheet forming the inner surface of 4, a flexible sheet having a gas barrier property is used. As a specific material, a plastic sheet having a high gas barrier property, for example, plain cellophane (PT), polyethylene terephthalate (PET), nylon (Ny), polypropylene (PP), polyethylene (PE), polyvinylidene chloride (PVDC) is used. ) And other films and these, or a composite film with other films and paper. As an example of the composite film, PT / P can be obtained by using the symbols in the above parentheses.
E, PVDC coat / PT / PE, PET / PE, polycarbonate / PE, PP (stretch) / PE / PT / P
E, PVDC / PET / PE, PVC (non-plastic) / P
E, PP (stretch) / PVA / PE, PP / PE / PVD
C, Ny / PE, etc. When the plastic film and the composite film described above are used, the containers 1 and 6 can be made transparent by making each film transparent, so that the coloration can be observed from the outside and the urine collection amount is easy to see. Further, also in the container 14, if a part of the paperboard is hollowed out in a small window shape, it is possible to see through. Alternatively, if a composite of a plastic film and an aluminum foil (Al) is used instead of the composite film, the gas barrier property is further improved. In this example, PT
/ PE / Al / PE, Ny / PE / Al / PE, etc., and each of the above films and composite films having a necessary gas barrier property is appropriately selected and used according to the application.

The urine test reagent area 4 causes a change in color tone due to pathological components in urine, such as sugar, protein, occult blood, ketone bodies (acetoacetic acid or acetone), urobilinogen, bilirubin, etc. or pH of urine. It is an area. The change in color tone occurs when the dye or the dye precursor contained in the reagent changes color or develops due to the reaction between the pathological component and the reagent. The following table shows specific pathological components and reagents used therefor.

[0018]

[Table 1]

For example, in the above table, when sugar is detected, glucose in urine is oxidized with glucose oxidase, and hydrogen peroxide generated at this time oxidizes and colors O-tolidine in the presence of peroxidase. Turn blue-purple.

In the case of protein, the binding of albumin with tetrabromophenol blue causes the color of tetrabromophenol blue to change from yellow to blue. As for pH, as is well known, acid / base indicators are used.

Since occult blood has hemoglobin, O-tolidine is oxidized and colored in the presence of peroxide and O-tolidine by utilizing its peroxidase activity. The color tone is similar to sugar. Ketone turns colorless to purple when acetoacetic acid or acetone in urine reacts with sodium nitroprusside.

With respect to urobilinogen, a red color is produced by the reaction between urobilinogen in the urine and p-dimethylaminobenzaldehyde, and with respect to bilirubin, a dye is produced by coupling reaction with a dichlorobenzenediazonium salt to give a red color.

In order to form the urine test reagent area using such an indicator or enzyme, a composition containing a desired indicator or enzyme is prepared, and the composition is applied to a predetermined portion on the inner surface of the container. The coating method may be any method such as a printing method and various coating methods. Particularly, the printing method is suitable for mass production because of its excellent manufacturing efficiency. The timing for forming the urine test reagent area on the inner surface of the container may be before or after the container is assembled.

It is desirable that all the urine test reagents used to form the urine test reagent area by the printing method are formed by using a non-aqueous solvent in terms of manufacturing efficiency. Among these, glucose oxidase and peroxidase are especially used. The non-aqueous solvent type sugar detection reagent used is superior to the composition using an aqueous solvent (Japanese Patent Application No. 57-90664) in terms of enzyme stability, production efficiency, sugar content of printed matter, and reliability. However, it is even better to use the present invention.

The components of the composition in the non-aqueous solvent are the above-mentioned enzyme, the indicator, the binder and the additive, of which the enzyme is not necessary when detecting other than sugar. As the binder in the composition, it is necessary that it does not affect the target components in urine or urine and the above-mentioned enzyme and indicator, and does not interfere with the test, and an appropriate test is performed each time. It is desirable to check and use by the method, but according to experiments by the present inventors, the following polymer compounds can be used.

As synthetic polymers, polyacrylic acid ester, polyphenylacetal, polymethacrylate, polyacrylamide, polyurethane, polyvinyl chloride, polystyrene, polymers of maleic acid and other components, polyvinyl acetate, polyvinyl alcohol, polyvinyl. Semi-synthetic polymers such as pyrrolidone, cellulose derivatives such as methyl cellulose, ethyl cellulose, and propyl cellulose; starch ethers; natural polymers such as starch, casein, and polysaccharides.

Among the above-mentioned polymer compounds, it is more preferable to use maleic acid type copolymers containing maleic acid as a component such as isobutylene-maleic anhydride copolymer and styrene-maleic acid copolymer.

In addition to the above-mentioned enzyme, indicator and binder, in order to improve the water absorption and water retention of the test area as an additive and to smoothly promote the reaction between the enzyme and indicator and pathological components in urine. , As a water-absorbing carrier, silica-based clay, calcium carbonate, aluminum silicate, glass,
Fine particles such as cellulose are appropriately added.

A method for preparing a non-aqueous solvent-based composition using each of the above components will be described. First, the freeze-dried enzyme, the indicator, and the buffer, if necessary, are pulverized to about 2
The particle size is 5 μm or less. Next, the enzyme, the indicator, the particles of the buffer, in the binder solution in which the binder is previously dissolved in a non-aqueous solvent, dispersed with the fine particles as a water-absorbing carrier,
Knead. A high-speed stirrer, a sand mill, a ball mill, a homogenizer, three rolls, an ultrasonic disperser, etc. are used for dispersion and kneading.

The non-aqueous solvent may be selected from organic solvents such as alcohols, ketones, aromatics, various esters and hydrocarbons in consideration of solubility of the binder. However, it is preferable to dehydrate the organic solvent in advance before use. When a composition is prepared for the purpose of detecting sugar, the use of methanol as a solvent is not preferable because the enzyme activity is lost more rapidly than when water is used. Further, if necessary, a surfactant or the like may be added to the composition in order to improve water wettability and uniformity of color development. As the surfactant, any of anionic, cationic and nonionic surfactants can be used.

Any known printing method can be used for the purpose of printing on the inner surface of the container or another substrate to be attached later on the inner surface of the container using such a composition, but in the present invention, the coating amount is relatively large. A method in which the amount of coating is large and the coating amount is constant is preferable, and in that sense, silk screen printing, intaglio printing, gravure printing and the like are preferable. Although the coating amount cannot be generally stated, it is usually 2 g / m ^{2 to} 30 g / m ² (when dried). Another substrate that does not react with the reagent,
Any substance may be used as long as it does not inhibit the reaction of the reagent in the color development process. For example, paper, synthetic paper, various types of plastics and films, and a laminate of paper and plastic can be used.

The present invention basically has the above-mentioned structure, but the following modifications may be made. For example, the urine test reagent area 4 may be several areas in which the test target substance is changed, or it may be a urine test reagent area in which the concentration can be determined. You may make it a symbol, especially a determination symbol, appear.

In the example of FIGS. 1 to 9, the urine test reagent area is an opening if the gas barrier sheet is not transparent,
That is, the coloration state is determined when viewed from above. However, in any of the examples, a small window for observation or the like may be provided above the container so that the colored state can be seen from the lateral direction (FIGS. 10 to 12).

FIG. 10 shows an example of the shape of the container 38 suitable for lateral observation after opening, in which a notch 41 is formed on the front side 39 of the container 38. In the case of this shape, for example, in the case of the container of FIGS. 1 and 2, perforations are provided along the line 41 of FIG. 10, and the lines 41 are sandwiched to strengthen both sides in parallel. Then, it can be formed by opening. The shape shown in FIG. 10 may be used as it is, but once the urine is collected, the urine test reagent area 4 is colored, the contents are discarded, and the urine test reagent area 4 is refolded and provided in advance if necessary. Compared with the standard color 40, the colored area 4 and the standard color 40 are arranged side by side, which is easy to compare. Standard color 40
Are provided in the required number depending on the substance to be inspected and its concentration.

FIG. 11 also shows an example of a container suitable for lateral observation, in which a small window 44 is formed of a transparent sheet on the upper side of the front side 43 of the container 42, and the inside of the urine test reagent area 1 is formed.
This is an example in which 0 is provided. As a method of opening the small window 44 and providing the urine test reagent area 4, if a part of the material forming the front side 43 is a composite film, paper or aluminum is partially removed, or a part thereof is not colored. By making it small window-shaped transparent, or by hollowing out only the surface and forming an inner surface with a transparent sheet at least at a portion corresponding to the hollowed-out portion to form a small window-shaped composition containing a urine test reagent in that portion. There is a method in which the urine test reagent area 4 is provided by applying.
In the example of FIG. 11, if the standard colors 40 are formed side by side as necessary, it is easy to compare the coloration of the area 4 with the standard colors 40 without folding the container.

In FIG. 12, a small window 44 is opened on the front side 46 of the container 45 and is made of a transparent sheet similar to that of FIG. 11, and the urine test reagent area 4 is provided on the inner surface of the back side 47. Even with such a shape, coloration can be observed from the lateral direction.

In the above description, it is mentioned that the standard color is provided on the surface of the container in advance. However, the standard color may be provided on a base material different from the container, or the standard color may be provided on the inner surface of the container for urinalysis. It may be formed side by side with the reagent area 4. When it is formed side by side on the inner surface of the container, it may be formed of a composition that does not discolor by urine collection, or a protective layer may be provided after formation, and when the container is made of a composite film, the inside of the laminated It may be devised such as to be installed in.

Next, the present invention will be described in more detail by showing more concrete examples. Example 1 PVDC coated PET (thickness 30 μm) / PE (thickness 3
0 μm) gas barrier sheet is used.
A square pattern having a side of 6 mm was provided on the E side using the ink having the following composition.

Glucose composition Glucose oxidase ... 0.5 g (Toyobo, Grade II) Peroxidase ... 0.15 g (Toyobo, Grade III) O-tolidine ... 0.9 g Stearate monoglyceride・ ・ ・ ・ 1.0 g (Kato Soap, Atom T-95) isobutylene / maleic anhydride copolymer ・ ・ ・ 3.0 g (Kuraray isobutylene chemical, Isoban 60) n-butanol ・ ・ ・ ・ 60 g Microcrystals Cellulose ... 35 g (Avicel SF, manufactured by Asahi Kasei) In addition, a yellow or red coloring agent (dye or pigment) may be added to the above composition for glucose.

The above composition was finely dispersed by a homomixer and then printed by a screen printing method. The screen plate used was 100 mesh, and the total thickness of the resist and screen mesh was 100 μm. After printing, it was dried at 80 ° C. for 1 hour.

The obtained printing sheet was obtained by heat-sealing the bag as shown in FIG.
The test bag thus obtained was opened with the V-shaped notch on the upper side, and urine was collected. When the urine was brought into contact with the printed part, the printed part was colored.

Separately from this, the same printing was performed on a white polystyrene film, which was quickly dipped in urine having a known glucose concentration, and the color tone was measured after 30 seconds. The results are shown in the following table. The color tone in the following table is JIS
It is based on the standard color code specified in Z 8721.

From the following table it can be seen that a glucose concentration of 0.05% can be measured.

[0044]

[Table 2]

Further, in order to check the preservability of the inspection container, the container was left for 6 months under the conditions of temperature 40 ° C. and humidity 75% (RH).
When urine with a similar known glucose concentration was examined, the coloration state at a urinary glucose concentration of 0.1% was almost the same as the coloration state when used immediately after printing,
It gave the same judgment value.

The same results as above can be obtained even if a container is made by using the composition for protein and the composition for pH shown below, and three compositions containing the composition for glucose are added. When the printed parts of each composition were separately arranged by using the products separately, three kinds of tests could be performed.

Composition for Protein Ethyl Cellulose (N-200): 3 g Ethyl alcohol / Methyl ethyl ketone: 76 g (3/7 mixed solvent) Tetrabromophenol blue :: 1 g Magnesium sulfate・ ・ ・ ・ 0.5 g citric acid ・ ・ ・ ・ ・ ・ 3 g sodium citrate ・ ・ ・ ・ 1.5 g Silica-based packing ・ ・ ・ ・ ・ 15 g (Crystallite FM-1 made by Tsuchiya Kaolin) Composition for pH Material Ethyl cellulose (N-50) ··· 8g Ethanol / toluene (2/8) mixed solvent ··· 76.89g Bromothymol blue ··· 0.1g Methyl red ··· 0.01g Silica-based filling Agent: 15 g (manufactured by Fuji Davison, Syloid 224) Example 2 PET (thickness 12 μm) / PE (thickness 15 μm) Al
A gas barrier sheet having a composition of (thickness 20 μm) / PE (thickness 30 μm) was used, and a printed portion was formed on the PE surface using the same composition as used in Example 1, and the self-standing bag shown in FIG. It was created. The obtained inspection bag can be used in the same manner as that obtained in Example 1 and, moreover, it can stand alone without any auxiliary means.

Example 3 Paper / PE (thickness: 30 g) was used with a paperboard having a basis weight of 200 g / m ^2.
μm) / PET (thickness 12 μm) / Al (thickness 9 μm)
The sheet shown in FIG. 6 was prepared using a sheet of / PE (thickness 20 μm). In this example, the same composition as used in Example 1 was used to form a printed portion on the inner surface of the container.

The container obtained in this example has a shape as shown in FIG. 7 when the upper part is opened at the position of the perforations and expanded, and the shape is maintained even if it is held by hand. Similar to Examples 1 and 2.

[0050]

In the urine test container of the present invention, since the urine test reagent area formed by applying the composition containing the test reagent is sealed in the container, the urine test container is used. There is an advantage that a container is not separately prepared, the reagent can be used in the urinalysis reagent area for a long period of time, and the volume is small, so that it does not take up space during storage.

[Brief description of drawings]

FIG. 1 is a plan view showing an embodiment of the present invention with a part cut away.

FIG. 2 is a plan view showing an embodiment of the present invention with a part cut away.

FIG. 3 is a cross-sectional view taken along the line AA of the container of FIG.

4 is a cross-sectional view of the container of FIG. 1 taken along the line BB.

FIG. 5 is a perspective view showing a state where the container of FIG. 2 is opened and stood upright.

FIG. 6 is a plan view showing an embodiment of the present invention with a part cut away.

FIG. 7 is a perspective view showing a state where the container of FIG. 6 is opened and expanded.

FIG. 8 is a perspective view showing a state where the container of FIG. 6 is opened and expanded.

FIG. 9 is an explanatory diagram showing a developed state of the container of FIG.

FIG. 10 is a perspective view partially showing another embodiment of the container of the present invention.

FIG. 11 is a perspective view partially showing another embodiment of the container of the present invention.

FIG. 12 is a perspective view partially showing another embodiment of the container of the present invention.

[Explanation of symbols]

1, 6, 14, 38, 42, 45 ... Container 2, 41 ... Notched line 7 ... Front side sheet 8 ... Back side sheet 9 ... Bottom sheet 15, 16, 17, 18 ... Perforation 40 ... Standard color 44 ... Small window

Claims (4)

[Claims] 1. A urine test reagent zone is formed by applying a composition containing a urine test reagent to the inner surface of a container configured using a gas barrier sheet, and the opening of the container is sealed. A container for urine test characterized in that the inside of the container is sealed by. 2. The urine test container according to claim 1, wherein the container is a packaging bag. 3. The urine test container according to claim 1, wherein the container is a self-supporting container. 4. The container for urinalysis according to claim 1, wherein the container is a packaging bag made of a flexible sheet, the periphery of which is reinforced with a rigid material.