JPH0528600B2 - - Google Patents

Info

Publication number: JPH0528600B2
Authority: JP; Japan
Prior art keywords: sample; qualitative; viral genes; quantitative determination; dna
Prior art date: 1986-10-27
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

JP62125633A

Other languages

English (en)

Japanese (ja)

Other versions

JPS63214200A (ja

Inventor

Seisen Jo

Yoshihiro Kin

Shichiryu Kyo

Zaikaku Ri

Joju In

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

BOKUGAN SEIMEI KOGAKU KENKYUSHO

Original Assignee

BOKUGAN SEIMEI KOGAKU KENKYUSHO

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1986-10-27

Filing date

1987-05-22

Publication date

1993-04-26

1987-05-22 Application filed by BOKUGAN SEIMEI KOGAKU KENKYUSHO filed Critical BOKUGAN SEIMEI KOGAKU KENKYUSHO

1988-09-06 Publication of JPS63214200A publication Critical patent/JPS63214200A/ja

1993-04-26 Publication of JPH0528600B2 publication Critical patent/JPH0528600B2/ja

Status Granted legal-status Critical Current

Links

239000000523 sample Substances 0.000 claims description 63
238000000034 method Methods 0.000 claims description 57
239000000243 solution Substances 0.000 claims description 36
108090000623 proteins and genes Proteins 0.000 claims description 34
239000002202 Polyethylene glycol Substances 0.000 claims description 31
108700005077 Viral Genes Proteins 0.000 claims description 31
229920001223 polyethylene glycol Polymers 0.000 claims description 31
239000003298 DNA probe Substances 0.000 claims description 24
241000700605 Viruses Species 0.000 claims description 24
239000007864 aqueous solution Substances 0.000 claims description 21
108020003215 DNA Probes Proteins 0.000 claims description 20
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 16
239000002245 particle Substances 0.000 claims description 15
239000002244 precipitate Substances 0.000 claims description 15
210000002966 serum Anatomy 0.000 claims description 15
238000005119 centrifugation Methods 0.000 claims description 14
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 12
239000013641 positive control Substances 0.000 claims description 12
239000000284 extract Substances 0.000 claims description 11
230000005855 radiation Effects 0.000 claims description 10
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 9
238000009396 hybridization Methods 0.000 claims description 9
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
108020005202 Viral DNA Proteins 0.000 claims description 7
-1 polyoxyethylene octylphenyl ether Polymers 0.000 claims description 6
210000003296 saliva Anatomy 0.000 claims description 6
210000002700 urine Anatomy 0.000 claims description 6
230000002285 radioactive effect Effects 0.000 claims description 5
239000012266 salt solution Substances 0.000 claims description 5
239000012153 distilled water Substances 0.000 claims description 4
238000005259 measurement Methods 0.000 claims description 3
238000002156 mixing Methods 0.000 claims description 3
229920002114 octoxynol-9 Polymers 0.000 claims description 3
239000007984 Tris EDTA buffer Substances 0.000 claims description 2
230000009089 cytolysis Effects 0.000 claims description 2
238000011002 quantification Methods 0.000 claims description 2
230000001376 precipitating effect Effects 0.000 claims 1
239000000020 Nitrocellulose Substances 0.000 description 50
229920001220 nitrocellulos Polymers 0.000 description 50
239000012528 membrane Substances 0.000 description 49
108020004414 DNA Proteins 0.000 description 30
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 22
235000002639 sodium chloride Nutrition 0.000 description 15
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 14
239000007790 solid phase Substances 0.000 description 14
108010067770 Endopeptidase K Proteins 0.000 description 11
239000006228 supernatant Substances 0.000 description 11
241000700721 Hepatitis B virus Species 0.000 description 9
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
239000000126 substance Substances 0.000 description 9
238000006243 chemical reaction Methods 0.000 description 8
150000003839 salts Chemical class 0.000 description 8
238000011160 research Methods 0.000 description 7
239000012488 sample solution Substances 0.000 description 7
239000011780 sodium chloride Substances 0.000 description 7
229920002554 vinyl polymer Polymers 0.000 description 7
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 6
238000002835 absorbance Methods 0.000 description 6
238000000376 autoradiography Methods 0.000 description 6
239000010410 layer Substances 0.000 description 6
238000012360 testing method Methods 0.000 description 6
108020004511 Recombinant DNA Proteins 0.000 description 5
102000004169 proteins and genes Human genes 0.000 description 5
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 4
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 4
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
239000000872 buffer Substances 0.000 description 4
238000000605 extraction Methods 0.000 description 4
229920003023 plastic Polymers 0.000 description 4
239000004033 plastic Substances 0.000 description 4
229920006395 saturated elastomer Polymers 0.000 description 4
238000012546 transfer Methods 0.000 description 4
229930006000 Sucrose Natural products 0.000 description 3
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
238000011161 development Methods 0.000 description 3
230000000694 effects Effects 0.000 description 3
238000005516 engineering process Methods 0.000 description 3
239000000203 mixture Substances 0.000 description 3
239000013642 negative control Substances 0.000 description 3
238000011084 recovery Methods 0.000 description 3
239000005720 sucrose Substances 0.000 description 3
238000005199 ultracentrifugation Methods 0.000 description 3
238000003260 vortexing Methods 0.000 description 3
238000005406 washing Methods 0.000 description 3
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical group N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
BHNQPLPANNDEGL-UHFFFAOYSA-N 2-(4-octylphenoxy)ethanol Chemical compound CCCCCCCCC1=CC=C(OCCO)C=C1 BHNQPLPANNDEGL-UHFFFAOYSA-N 0.000 description 2
241000972773 Aulopiformes Species 0.000 description 2
AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 description 2
RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
102000006382 Ribonucleases Human genes 0.000 description 2
108010083644 Ribonucleases Proteins 0.000 description 2
240000004808 Saccharomyces cerevisiae Species 0.000 description 2
239000007983 Tris buffer Substances 0.000 description 2
238000007605 air drying Methods 0.000 description 2
238000003556 assay Methods 0.000 description 2
238000011088 calibration curve Methods 0.000 description 2
SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 2
HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 2
NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 2
238000005755 formation reaction Methods 0.000 description 2
238000011534 incubation Methods 0.000 description 2
PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
238000002955 isolation Methods 0.000 description 2
238000002372 labelling Methods 0.000 description 2
239000007788 liquid Substances 0.000 description 2
239000012139 lysis buffer Substances 0.000 description 2
229910001629 magnesium chloride Inorganic materials 0.000 description 2
235000011147 magnesium chloride Nutrition 0.000 description 2
238000004519 manufacturing process Methods 0.000 description 2
244000005700 microbiome Species 0.000 description 2
238000006386 neutralization reaction Methods 0.000 description 2
230000003472 neutralizing effect Effects 0.000 description 2
235000019515 salmon Nutrition 0.000 description 2
239000000725 suspension Substances 0.000 description 2
238000013519 translation Methods 0.000 description 2
LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
108090001008 Avidin Proteins 0.000 description 1
241000193830 Bacillus <bacterium> Species 0.000 description 1
241000894006 Bacteria Species 0.000 description 1
108091003079 Bovine Serum Albumin Proteins 0.000 description 1
KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
102000053602 DNA Human genes 0.000 description 1
102000012410 DNA Ligases Human genes 0.000 description 1
108010061982 DNA Ligases Proteins 0.000 description 1
ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 description 1
241001524679 Escherichia virus M13 Species 0.000 description 1
229920001917 Ficoll Polymers 0.000 description 1
241001465754 Metazoa Species 0.000 description 1
241001181114 Neta Species 0.000 description 1
229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
108020004518 RNA Probes Proteins 0.000 description 1
239000003391 RNA probe Substances 0.000 description 1
108010077895 Sarcosine Proteins 0.000 description 1
108020004682 Single-Stranded DNA Proteins 0.000 description 1
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
108020004566 Transfer RNA Proteins 0.000 description 1
108020000999 Viral RNA Proteins 0.000 description 1
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
229910052921 ammonium sulfate Inorganic materials 0.000 description 1
235000011130 ammonium sulphate Nutrition 0.000 description 1
239000000427 antigen Substances 0.000 description 1
102000036639 antigens Human genes 0.000 description 1
108091007433 antigens Proteins 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
229960002685 biotin Drugs 0.000 description 1
235000020958 biotin Nutrition 0.000 description 1
239000011616 biotin Substances 0.000 description 1
239000012888 bovine serum Substances 0.000 description 1
229940098773 bovine serum albumin Drugs 0.000 description 1
239000007853 buffer solution Substances 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
238000004140 cleaning Methods 0.000 description 1
230000000052 comparative effect Effects 0.000 description 1
230000000295 complement effect Effects 0.000 description 1
238000011109 contamination Methods 0.000 description 1
229920001577 copolymer Polymers 0.000 description 1
238000005520 cutting process Methods 0.000 description 1
238000004925 denaturation Methods 0.000 description 1
230000036425 denaturation Effects 0.000 description 1
238000001514 detection method Methods 0.000 description 1
201000010099 disease Diseases 0.000 description 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
238000004090 dissolution Methods 0.000 description 1
238000004980 dosimetry Methods 0.000 description 1
229940079593 drug Drugs 0.000 description 1
239000003814 drug Substances 0.000 description 1
DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
239000007850 fluorescent dye Substances 0.000 description 1
239000012634 fragment Substances 0.000 description 1
238000010359 gene isolation Methods 0.000 description 1
150000004676 glycans Chemical class 0.000 description 1
238000000703 high-speed centrifugation Methods 0.000 description 1
VEXZGXHMUGYJMC-UHFFFAOYSA-N hydrochloric acid Substances Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
230000003100 immobilizing effect Effects 0.000 description 1
239000012535 impurity Substances 0.000 description 1
238000000691 measurement method Methods 0.000 description 1
125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
239000012044 organic layer Substances 0.000 description 1
150000002989 phenols Chemical class 0.000 description 1
239000013612 plasmid Substances 0.000 description 1
229920001282 polysaccharide Polymers 0.000 description 1
239000005017 polysaccharide Substances 0.000 description 1
229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
239000001267 polyvinylpyrrolidone Substances 0.000 description 1
235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
229910000160 potassium phosphate Inorganic materials 0.000 description 1
235000011009 potassium phosphates Nutrition 0.000 description 1
239000000047 product Substances 0.000 description 1
238000000163 radioactive labelling Methods 0.000 description 1
108091008146 restriction endonucleases Proteins 0.000 description 1
238000005185 salting out Methods 0.000 description 1
229920006298 saran Polymers 0.000 description 1
108700004121 sarkosyl Proteins 0.000 description 1
229940016590 sarkosyl Drugs 0.000 description 1
239000013049 sediment Substances 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
230000000405 serological effect Effects 0.000 description 1
239000011734 sodium Substances 0.000 description 1
239000001632 sodium acetate Substances 0.000 description 1
235000017281 sodium acetate Nutrition 0.000 description 1
KSAVQLQVUXSOCR-UHFFFAOYSA-M sodium lauroyl sarcosinate Chemical compound [Na+].CCCCCCCCCCCC(=O)N(C)CC([O-])=O KSAVQLQVUXSOCR-UHFFFAOYSA-M 0.000 description 1
239000001488 sodium phosphate Substances 0.000 description 1
229910000162 sodium phosphate Inorganic materials 0.000 description 1
229940048098 sodium sarcosinate Drugs 0.000 description 1
229940074404 sodium succinate Drugs 0.000 description 1
ZDQYSKICYIVCPN-UHFFFAOYSA-L sodium succinate (anhydrous) Chemical compound [Na+].[Na+].[O-]C(=O)CCC([O-])=O ZDQYSKICYIVCPN-UHFFFAOYSA-L 0.000 description 1
238000000638 solvent extraction Methods 0.000 description 1
238000013518 transcription Methods 0.000 description 1
230000035897 transcription Effects 0.000 description 1
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
230000007501 viral attachment Effects 0.000 description 1
DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Immunology (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
Hematology (AREA)
Urology & Nephrology (AREA)
Biochemistry (AREA)
Analytical Chemistry (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Physics & Mathematics (AREA)
Organic Chemistry (AREA)
General Health & Medical Sciences (AREA)
Wood Science & Technology (AREA)
Food Science & Technology (AREA)
Pathology (AREA)
Zoology (AREA)
General Physics & Mathematics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Cell Biology (AREA)
Medicinal Chemistry (AREA)
Genetics & Genomics (AREA)
Virology (AREA)
Biophysics (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)

JP62125633A 1986-10-27 1987-05-22 ウィルス遺伝子の定性・定量方法及びそのキット Granted JPS63214200A (ja)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
KR1019860008975A KR900001577B1 (ko)	1986-10-27	1986-10-27	폴리에틸렌 글리콜을 이용하여 시료중에 존재하는 바이러스 유전자의 정량방법 및 그의 키트
KR8975		1986-10-27

Publications (2)

Publication Number	Publication Date
JPS63214200A JPS63214200A (ja)	1988-09-06
JPH0528600B2 true JPH0528600B2 (de)	1993-04-26

Family

ID=19252986

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP62125633A Granted JPS63214200A (ja)	1986-10-27	1987-05-22	ウィルス遺伝子の定性・定量方法及びそのキット

Country Status (3)

Country	Link
JP (1)	JPS63214200A (de)
KR (1)	KR900001577B1 (de)
DE (1)	DE3735877A1 (de)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4446237A (en) *	1981-03-27	1984-05-01	Life Technologies, Inc.	Method for detection of a suspect viral deoxyribonucleic acid in an acellular biological fluid

1986
- 1986-10-27 KR KR1019860008975A patent/KR900001577B1/ko not_active Expired
1987
- 1987-05-22 JP JP62125633A patent/JPS63214200A/ja active Granted
- 1987-10-23 DE DE19873735877 patent/DE3735877A1/de not_active Withdrawn

Also Published As

Publication number	Publication date
DE3735877A1 (de)	1988-05-05
KR880005460A (ko)	1988-06-29
JPS63214200A (ja)	1988-09-06
KR900001577B1 (ko)	1990-03-15

Publication	Publication Date	Title
CA1338207C (en)	1996-04-02	Method of assaying of nucleic acids, a reagent combination and kit therefore
CA1272443A (en)	1990-08-07	Solution-phase dual hybridization assay for detecting polynucleotide sequences
JP2552691B2 (ja)	1996-11-13	生物学的試料中の核酸評価のためのカオトロピック方法
EP0478319B1 (de)	1997-04-02	Verfahren zum Gennachweis
EP0439222B1 (de)	1995-10-25	Wasserunlösbares Reagenz, Nukleinsäure-Sonde, Testsatz und Verfahren zur Diagnose und Isolierung
EP0317074B1 (de)	1996-12-18	Hybridisierungsverfahren und Reagenzsatz dafür
US4562159A (en)	1985-12-31	Diagnostic test for hepatitis B virus
JP2690738B2 (ja)	1997-12-17	核酸の検出方法および装置
JPH04506599A (ja)	1992-11-19	特定の核酸配列を検出する方法及びその応用
AU5106990A (en)	1990-09-05	Detection of a nucleic acid sequence or a change therein
JP2006320326A (ja)	2006-11-30	特異的配列構成を有する核酸の検出法
EP1563100A2 (de)	2005-08-17	Verdrängungs-sandwich-immuno-pcr
Yourno et al.	1992	A novel polymerase chain reaction method for detection of human immunodeficiency virus in dried blood spots on filter paper
Ngamdee et al.	2020	Target Induced-DNA strand displacement reaction using gold nanoparticle labeling for hepatitis E virus detection
US6225053B1 (en)	2001-05-01	Detection of hepatitis B virus
JPH0743376B2 (ja)	1995-05-15	核酸配列の検定方法及び分子遺伝子プローブ
WO1992018649A1 (en)	1992-10-29	Compositions and methods for improved extraction and hybridization of nucleic acid
US8975017B2 (en)	2015-03-10	Process for concentrating nucleic acid molecules
JPH0528600B2 (de)	1993-04-26
JPS61293400A (ja)	1986-12-24	ポリヌクレオチド配列の測定方法
KR900005337B1 (ko)	1990-07-27	바이러스 유전자의 정성 · 정량방법 및 그의 키트
Harding et al.	1989	Rapid isolation of DNA from complex biological samples ustng a novel capture reagent—methidium spermine-sepharose
US20040185449A1 (en)	2004-09-23	Method for preparing assay samples
Tu et al.	2004	The application of alkaline phosphatase labeled HBV probe in serum detection
JPH07298897A (ja)	1995-11-14	２つの標識プライマーを用いた標的核酸の生成、増幅及び検出方法並びにそれらを含有するｐｃｒキット