JPH054079B2 - - Google Patents

Info

Publication number: JPH054079B2
Authority: JP; Japan
Prior art keywords: bilirubin; oxidase; novel; enzyme; composition
Prior art date: 1984-05-24
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

JP10560484A

Other languages

English (en)

Japanese (ja)

Other versions

JPS60249060A (ja

Inventor

Yoshio Yoshihama

Susumu Matsui

Tsutomu Taniguchi

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Takara Shuzo Co Ltd

Original Assignee

Takara Shuzo Co Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-05-24

Filing date

1984-05-24

Publication date

1993-01-19

1984-05-24 Application filed by Takara Shuzo Co Ltd filed Critical Takara Shuzo Co Ltd

1984-05-24 Priority to JP10560484A priority Critical patent/JPS60249060A/ja

1984-09-10 Priority to US06/649,054 priority patent/US4600689A/en

1984-09-11 Priority to GB08422935A priority patent/GB2146997B/en

1984-09-24 Priority to CA000463878A priority patent/CA1219792A/en

1984-09-26 Priority to KR1019840005904A priority patent/KR880000753B1/ko

1984-10-01 Priority to DE3436005A priority patent/DE3436005C2/de

1984-10-19 Priority to FR8416064A priority patent/FR2555196B1/fr

1985-12-09 Publication of JPS60249060A publication Critical patent/JPS60249060A/ja

1993-01-19 Publication of JPH054079B2 publication Critical patent/JPH054079B2/ja

Status Granted legal-status Critical Current

Links

BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 claims description 206
108010015428 Bilirubin oxidase Proteins 0.000 claims description 58
238000000034 method Methods 0.000 claims description 43
239000000203 mixture Substances 0.000 claims description 28
239000003153 chemical reaction reagent Substances 0.000 claims description 23
KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 claims description 19
238000006243 chemical reaction Methods 0.000 claims description 18
238000004458 analytical method Methods 0.000 claims description 9
239000003112 inhibitor Substances 0.000 claims description 7
238000012360 testing method Methods 0.000 claims description 7
239000012085 test solution Substances 0.000 claims description 5
108090000854 Oxidoreductases Proteins 0.000 claims description 4
102000004316 Oxidoreductases Human genes 0.000 claims description 4
230000002401 inhibitory effect Effects 0.000 claims description 4
238000004445 quantitative analysis Methods 0.000 claims description 4
239000007788 liquid Substances 0.000 claims description 3
229940009976 deoxycholate Drugs 0.000 claims 3
102000004190 Enzymes Human genes 0.000 description 44
108090000790 Enzymes Proteins 0.000 description 44
229940088598 enzyme Drugs 0.000 description 44
230000000694 effects Effects 0.000 description 24
239000000243 solution Substances 0.000 description 24
210000001124 body fluid Anatomy 0.000 description 21
239000010839 body fluid Substances 0.000 description 21
238000002835 absorbance Methods 0.000 description 20
210000002966 serum Anatomy 0.000 description 20
MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 14
HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 14
229960003964 deoxycholic acid Drugs 0.000 description 14
108010088751 Albumins Proteins 0.000 description 11
102000009027 Albumins Human genes 0.000 description 11
PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 10
238000004040 coloring Methods 0.000 description 9
238000005259 measurement Methods 0.000 description 9
239000008057 potassium phosphate buffer Substances 0.000 description 9
239000000126 substance Substances 0.000 description 9
RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 8
238000004440 column chromatography Methods 0.000 description 8
238000010186 staining Methods 0.000 description 7
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
229920005654 Sephadex Polymers 0.000 description 6
239000012507 Sephadex™ Substances 0.000 description 6
239000008103 glucose Substances 0.000 description 6
210000002700 urine Anatomy 0.000 description 6
238000010521 absorption reaction Methods 0.000 description 5
102000004169 proteins and genes Human genes 0.000 description 5
108090000623 proteins and genes Proteins 0.000 description 5
108010089254 Cholesterol oxidase Proteins 0.000 description 4
108010055297 Sterol Esterase Proteins 0.000 description 4
102000000019 Sterol Esterase Human genes 0.000 description 4
-1 potassium ferricyanide Chemical compound 0.000 description 4
GWZYPXHJIZCRAJ-UHFFFAOYSA-N Biliverdin Natural products CC1=C(C=C)C(=C/C2=NC(=Cc3[nH]c(C=C/4NC(=O)C(=C4C)C=C)c(C)c3CCC(=O)O)C(=C2C)CCC(=O)O)NC1=O GWZYPXHJIZCRAJ-UHFFFAOYSA-N 0.000 description 3
RCNSAJSGRJSBKK-NSQVQWHSSA-N Biliverdin IX Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(\C=C/2C(=C(C)C(=C/C=3C(=C(C=C)C(=O)N=3)C)/N\2)CCC(O)=O)N1 RCNSAJSGRJSBKK-NSQVQWHSSA-N 0.000 description 3
RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 3
AEMOLEFTQBMNLQ-AQKNRBDQSA-N D-glucopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-AQKNRBDQSA-N 0.000 description 3
IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 3
108010015776 Glucose oxidase Proteins 0.000 description 3
239000004366 Glucose oxidase Substances 0.000 description 3
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 3
QBUVFDKTZJNUPP-UHFFFAOYSA-N biliverdin-IXalpha Natural products N1C(=O)C(C)=C(C=C)C1=CC1=C(C)C(CCC(O)=O)=C(C=C2C(=C(C)C(C=C3C(=C(C=C)C(=O)N3)C)=N2)CCC(O)=O)N1 QBUVFDKTZJNUPP-UHFFFAOYSA-N 0.000 description 3
238000011088 calibration curve Methods 0.000 description 3
229910052802 copper Inorganic materials 0.000 description 3
239000010949 copper Substances 0.000 description 3
239000012153 distilled water Substances 0.000 description 3
238000001962 electrophoresis Methods 0.000 description 3
239000012530 fluid Substances 0.000 description 3
230000002538 fungal effect Effects 0.000 description 3
229940116332 glucose oxidase Drugs 0.000 description 3
235000019420 glucose oxidase Nutrition 0.000 description 3
229940097043 glucuronic acid Drugs 0.000 description 3
230000005764 inhibitory process Effects 0.000 description 3
238000000691 measurement method Methods 0.000 description 3
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
241000221198 Basidiomycota Species 0.000 description 2
QIGBRXMKCJKVMJ-UHFFFAOYSA-N Hydroquinone Chemical compound OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 description 2
239000001888 Peptone Substances 0.000 description 2
108010080698 Peptones Proteins 0.000 description 2
238000000862 absorption spectrum Methods 0.000 description 2
239000000654 additive Substances 0.000 description 2
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
239000007853 buffer solution Substances 0.000 description 2
YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical compound OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 description 2
239000007795 chemical reaction product Substances 0.000 description 2
239000003795 chemical substances by application Substances 0.000 description 2
235000012000 cholesterol Nutrition 0.000 description 2
229910021645 metal ion Inorganic materials 0.000 description 2
230000001590 oxidative effect Effects 0.000 description 2
239000001301 oxygen Substances 0.000 description 2
229910052760 oxygen Inorganic materials 0.000 description 2
235000019319 peptone Nutrition 0.000 description 2
229920002401 polyacrylamide Polymers 0.000 description 2
238000002360 preparation method Methods 0.000 description 2
WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 2
238000011002 quantification Methods 0.000 description 2
239000002683 reaction inhibitor Substances 0.000 description 2
230000002829 reductive effect Effects 0.000 description 2
NRHMKIHPTBHXPF-TUJRSCDTSA-M sodium cholate Chemical compound [Na+].C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC([O-])=O)C)[C@@]2(C)[C@@H](O)C1 NRHMKIHPTBHXPF-TUJRSCDTSA-M 0.000 description 2
PUZPDOWCWNUUKD-UHFFFAOYSA-M sodium fluoride Chemical compound [F-].[Na+] PUZPDOWCWNUUKD-UHFFFAOYSA-M 0.000 description 2
239000004094 surface-active agent Substances 0.000 description 2
238000000108 ultra-filtration Methods 0.000 description 2
CBCKQZAAMUWICA-UHFFFAOYSA-N 1,4-phenylenediamine Chemical compound NC1=CC=C(N)C=C1 CBCKQZAAMUWICA-UHFFFAOYSA-N 0.000 description 1
PWKSKIMOESPYIA-UHFFFAOYSA-N 2-acetamido-3-sulfanylpropanoic acid Chemical compound CC(=O)NC(CS)C(O)=O PWKSKIMOESPYIA-UHFFFAOYSA-N 0.000 description 1
ZKZNWRUUTQNYTH-UHFFFAOYSA-N 4-azidobenzenesulfonic acid Chemical compound OS(=O)(=O)C1=CC=C(N=[N+]=[N-])C=C1 ZKZNWRUUTQNYTH-UHFFFAOYSA-N 0.000 description 1
244000251953 Agaricus brunnescens Species 0.000 description 1
235000001674 Agaricus brunnescens Nutrition 0.000 description 1
241001660906 Agrocybe pediades Species 0.000 description 1
241000700199 Cavia porcellus Species 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
241000127897 Ganoderma tsunodae Species 0.000 description 1
102000003886 Glycoproteins Human genes 0.000 description 1
108090000288 Glycoproteins Proteins 0.000 description 1
102000001554 Hemoglobins Human genes 0.000 description 1
108010054147 Hemoglobins Proteins 0.000 description 1
108010001336 Horseradish Peroxidase Proteins 0.000 description 1
239000002211 L-ascorbic acid Substances 0.000 description 1
235000000069 L-ascorbic acid Nutrition 0.000 description 1
102000003992 Peroxidases Human genes 0.000 description 1
241000222481 Schizophyllum commune Species 0.000 description 1
229920002472 Starch Polymers 0.000 description 1
241000222355 Trametes versicolor Species 0.000 description 1
239000013504 Triton X-100 Substances 0.000 description 1
229920004890 Triton X-100 Polymers 0.000 description 1
240000008042 Zea mays Species 0.000 description 1
235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
235000002017 Zea mays subsp mays Nutrition 0.000 description 1
229910052783 alkali metal Inorganic materials 0.000 description 1
150000001340 alkali metals Chemical class 0.000 description 1
229910052784 alkaline earth metal Inorganic materials 0.000 description 1
229960005070 ascorbic acid Drugs 0.000 description 1
238000003556 assay Methods 0.000 description 1
210000004556 brain Anatomy 0.000 description 1
239000000872 buffer Substances 0.000 description 1
239000000969 carrier Substances 0.000 description 1
229920001429 chelating resin Polymers 0.000 description 1
239000003638 chemical reducing agent Substances 0.000 description 1
AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 description 1
235000005822 corn Nutrition 0.000 description 1
235000012343 cottonseed oil Nutrition 0.000 description 1
239000002385 cottonseed oil Substances 0.000 description 1
230000003247 decreasing effect Effects 0.000 description 1
KXGVEGMKQFWNSR-UHFFFAOYSA-N deoxycholic acid Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 KXGVEGMKQFWNSR-UHFFFAOYSA-N 0.000 description 1
230000009034 developmental inhibition Effects 0.000 description 1
125000000664 diazo group Chemical group [N-]=[N+]=[*] 0.000 description 1
239000012954 diazonium Substances 0.000 description 1
150000001989 diazonium salts Chemical class 0.000 description 1
VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
238000003028 enzyme activity measurement method Methods 0.000 description 1
230000005284 excitation Effects 0.000 description 1
238000004108 freeze drying Methods 0.000 description 1
238000002523 gelfiltration Methods 0.000 description 1
229940025294 hemin Drugs 0.000 description 1
BTIJJDXEELBZFS-QDUVMHSLSA-K hemin Chemical compound CC1=C(CCC(O)=O)C(C=C2C(CCC(O)=O)=C(C)\C(N2[Fe](Cl)N23)=C\4)=N\C1=C/C2=C(C)C(C=C)=C3\C=C/1C(C)=C(C=C)C/4=N\1 BTIJJDXEELBZFS-QDUVMHSLSA-K 0.000 description 1
238000005470 impregnation Methods 0.000 description 1
238000009776 industrial production Methods 0.000 description 1
239000004615 ingredient Substances 0.000 description 1
238000009434 installation Methods 0.000 description 1
230000002452 interceptive effect Effects 0.000 description 1
239000002085 irritant Substances 0.000 description 1
231100000021 irritant Toxicity 0.000 description 1
238000012423 maintenance Methods 0.000 description 1
238000007726 management method Methods 0.000 description 1
239000007800 oxidant agent Substances 0.000 description 1
238000006395 oxidase reaction Methods 0.000 description 1
230000003647 oxidation Effects 0.000 description 1
238000007254 oxidation reaction Methods 0.000 description 1
108040007629 peroxidase activity proteins Proteins 0.000 description 1
OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
NNFCIKHAZHQZJG-UHFFFAOYSA-N potassium cyanide Chemical compound [K+].N#[C-] NNFCIKHAZHQZJG-UHFFFAOYSA-N 0.000 description 1
239000000276 potassium ferrocyanide Substances 0.000 description 1
230000001737 promoting effect Effects 0.000 description 1
229940079877 pyrogallol Drugs 0.000 description 1
235000013024 sodium fluoride Nutrition 0.000 description 1
239000011775 sodium fluoride Substances 0.000 description 1
239000003549 soybean oil Substances 0.000 description 1
235000012424 soybean oil Nutrition 0.000 description 1
239000003381 stabilizer Substances 0.000 description 1
235000019698 starch Nutrition 0.000 description 1
239000008107 starch Substances 0.000 description 1
239000000758 substrate Substances 0.000 description 1
238000010998 test method Methods 0.000 description 1
XOGGUFAVLNCTRS-UHFFFAOYSA-N tetrapotassium;iron(2+);hexacyanide Chemical compound [K+].[K+].[K+].[K+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] XOGGUFAVLNCTRS-UHFFFAOYSA-N 0.000 description 1
GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
239000011715 vitamin B12 Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
- C12Y103/03—Oxidoreductases acting on the CH-CH group of donors (1.3) with oxygen as acceptor (1.3.3)
- C12Y103/03005—Bilirubin oxidase (1.3.3.5)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Hematology (AREA)
General Health & Medical Sciences (AREA)
Immunology (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Analytical Chemistry (AREA)
Biomedical Technology (AREA)
Physics & Mathematics (AREA)
Microbiology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Genetics & Genomics (AREA)
Urology & Nephrology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Cell Biology (AREA)
Biophysics (AREA)
Food Science & Technology (AREA)
Medicinal Chemistry (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Investigating Or Analysing Biological Materials (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

JP10560484A 1983-11-21 1984-05-24 ビリルビン用試薬組成物及びその使用方法 Granted JPS60249060A (ja)

Priority Applications (7)

Application Number	Priority Date	Filing Date	Title
JP10560484A JPS60249060A (ja)	1984-05-24	1984-05-24	ビリルビン用試薬組成物及びその使用方法
US06/649,054 US4600689A (en)	1983-11-21	1984-09-10	Novel bilirubin oxidase, its production and use
GB08422935A GB2146997B (en)	1983-11-21	1984-09-11	Novel bilirubin oxidase, its production and use
CA000463878A CA1219792A (en)	1983-11-21	1984-09-24	Bilirubin oxidase, its production and use
KR1019840005904A KR880000753B1 (ko)	1983-11-21	1984-09-26	신규의 빌리루빈 산화효소를 포함하는 시약조성물 및 그의 사용방법
DE3436005A DE3436005C2 (de)	1983-11-21	1984-10-01	Neue Bilirubinoxidase, Verfahren zu ihrer Herstellung und diese enthaltende Reagenszubereitung
FR8416064A FR2555196B1 (fr)	1983-11-21	1984-10-19	Nouvelle bilirubine oxydase, son procede de production, composition reactive la contenant et son application au dosage de la bilirubine

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP10560484A JPS60249060A (ja)	1984-05-24	1984-05-24	ビリルビン用試薬組成物及びその使用方法

Publications (2)

Publication Number	Publication Date
JPS60249060A JPS60249060A (ja)	1985-12-09
JPH054079B2 true JPH054079B2 (de)	1993-01-19

Family

ID=14412104

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP10560484A Granted JPS60249060A (ja)	1983-11-21	1984-05-24	ビリルビン用試薬組成物及びその使用方法

Country Status (1)

Country	Link
JP (1)	JPS60249060A (de)

1984
- 1984-05-24 JP JP10560484A patent/JPS60249060A/ja active Granted

Also Published As

Publication number	Publication date
JPS60249060A (ja)	1985-12-09

Legal Events

Date	Code	Title	Description
2004-05-24	EXPY	Cancellation because of completion of term

Publication	Publication Date	Title
US4350762A (en)	1982-09-21	Aminopyrine improved Trinder's reagent and dosing process for hydrogen peroxide from enzymatic oxidation of metabolic substrata with the same
JP2610074B2 (ja)	1997-05-14	液体中のイオンを測定する方法及びそのための組成物
JPH0577399B2 (de)	1993-10-26
Matsubara et al.	1985	Spectrophotometric determination of hydrogen peroxide with titanium 2-((5-bromopyridyl) azo)-5-(N-propyl-N-sulfopropylamino) phenol reagent and its application to the determination of serum glucose using glucose oxidase
US6008006A (en)	1999-12-28	Determination of glycated proteins
GB2152215A (en)	1985-07-31	Method for the quantitative determination of physiological components in biological fluids
KR880000753B1 (ko)	1988-05-04	신규의 빌리루빈 산화효소를 포함하는 시약조성물 및 그의 사용방법
EP0100217A2 (de)	1984-02-08	Verfahren zur quantitativen Bestimmung eines mit Oxidase behandelten Substrates
EP0193204A2 (de)	1986-09-03	Verfahren zur Bestimmung der Superoxid-Dismutase-Aktivität
US4695539A (en)	1987-09-22	Process for quantitative determination of substrate treated with oxidase
JPH07155196A (ja)	1995-06-20	生体成分の測定法
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