JPH0542271B2 - - Google Patents
Info
- Publication number
- JPH0542271B2 JPH0542271B2 JP59037623A JP3762384A JPH0542271B2 JP H0542271 B2 JPH0542271 B2 JP H0542271B2 JP 59037623 A JP59037623 A JP 59037623A JP 3762384 A JP3762384 A JP 3762384A JP H0542271 B2 JPH0542271 B2 JP H0542271B2
- Authority
- JP
- Japan
- Prior art keywords
- nad
- compound
- diaphorase
- general formula
- peroxidase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 150000001875 compounds Chemical class 0.000 claims description 43
- 239000000243 solution Substances 0.000 claims description 29
- 238000000034 method Methods 0.000 claims description 18
- 102000003992 Peroxidases Human genes 0.000 claims description 12
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 12
- -1 diaphorase Proteins 0.000 claims description 9
- 238000002835 absorbance Methods 0.000 claims description 8
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 7
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 7
- 108091006149 Electron carriers Proteins 0.000 claims description 6
- 125000000217 alkyl group Chemical group 0.000 claims description 6
- 229910052739 hydrogen Inorganic materials 0.000 claims description 6
- 239000001257 hydrogen Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 125000002252 acyl group Chemical group 0.000 claims description 5
- 125000003342 alkenyl group Chemical group 0.000 claims description 5
- 125000003545 alkoxy group Chemical group 0.000 claims description 5
- 125000003118 aryl group Chemical group 0.000 claims description 5
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 4
- 229910052736 halogen Inorganic materials 0.000 claims description 3
- 150000002367 halogens Chemical class 0.000 claims description 3
- 125000000547 substituted alkyl group Chemical group 0.000 claims description 3
- 125000003107 substituted aryl group Chemical group 0.000 claims description 3
- 150000002431 hydrogen Chemical class 0.000 claims description 2
- 239000007864 aqueous solution Substances 0.000 claims 1
- 229950006238 nadide Drugs 0.000 description 29
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 22
- 238000006243 chemical reaction Methods 0.000 description 20
- 239000003153 chemical reaction reagent Substances 0.000 description 18
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 12
- 230000000694 effects Effects 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 8
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 239000002994 raw material Substances 0.000 description 8
- 239000000872 buffer Substances 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 6
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 6
- 239000013504 Triton X-100 Substances 0.000 description 6
- 229920004890 Triton X-100 Polymers 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 6
- 239000012153 distilled water Substances 0.000 description 6
- 210000002966 serum Anatomy 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 239000003613 bile acid Substances 0.000 description 5
- 238000011088 calibration curve Methods 0.000 description 5
- 239000003925 fat Substances 0.000 description 5
- 239000011521 glass Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 230000007935 neutral effect Effects 0.000 description 5
- FSVCQIDHPKZJSO-UHFFFAOYSA-L nitro blue tetrazolium dichloride Chemical compound [Cl-].[Cl-].COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 FSVCQIDHPKZJSO-UHFFFAOYSA-L 0.000 description 5
- 239000002244 precipitate Substances 0.000 description 5
- 230000035945 sensitivity Effects 0.000 description 5
- 101001083553 Homo sapiens Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial Proteins 0.000 description 4
- 102100029107 Long chain 3-hydroxyacyl-CoA dehydrogenase Human genes 0.000 description 4
- 102000004316 Oxidoreductases Human genes 0.000 description 4
- 108090000854 Oxidoreductases Proteins 0.000 description 4
- BOPGDPNILDQYTO-NDOGXIPWSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2r,3r,4r,5r)-5-(3-carbamoyl-4h-pyridin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl hydrogen phosphate Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NDOGXIPWSA-N 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N acetic acid Substances CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 4
- 150000001299 aldehydes Chemical class 0.000 description 4
- 125000004432 carbon atom Chemical group C* 0.000 description 4
- 238000004040 coloring Methods 0.000 description 4
- 235000019441 ethanol Nutrition 0.000 description 4
- 229930182830 galactose Natural products 0.000 description 4
- 239000000049 pigment Substances 0.000 description 4
- SMQUZDBALVYZAC-UHFFFAOYSA-N salicylaldehyde Chemical compound OC1=CC=CC=C1C=O SMQUZDBALVYZAC-UHFFFAOYSA-N 0.000 description 4
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical class O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 3
- NBSCHQHZLSJFNQ-GASJEMHNSA-N D-Glucose 6-phosphate Chemical compound OC1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H](O)[C@H]1O NBSCHQHZLSJFNQ-GASJEMHNSA-N 0.000 description 3
- 102100036504 Dehydrogenase/reductase SDR family member 9 Human genes 0.000 description 3
- VFRROHXSMXFLSN-UHFFFAOYSA-N Glc6P Natural products OP(=O)(O)OCC(O)C(O)C(O)C(O)C=O VFRROHXSMXFLSN-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 239000006173 Good's buffer Substances 0.000 description 3
- 108010013563 Lipoprotein Lipase Proteins 0.000 description 3
- 102000043296 Lipoprotein lipases Human genes 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 229940045189 glucose-6-phosphate Drugs 0.000 description 3
- VMGAPWLDMVPYIA-HIDZBRGKSA-N n'-amino-n-iminomethanimidamide Chemical compound N\N=C\N=N VMGAPWLDMVPYIA-HIDZBRGKSA-N 0.000 description 3
- 239000000376 reactant Substances 0.000 description 3
- BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 3
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 2
- ZYDGCYWJDWIJCS-UHFFFAOYSA-N 1-methoxyphenazine Chemical compound C1=CC=C2N=C3C(OC)=CC=CC3=NC2=C1 ZYDGCYWJDWIJCS-UHFFFAOYSA-N 0.000 description 2
- JIYCNHGAAQDQTN-UHFFFAOYSA-N 3-[(n-methylanilino)methyl]benzenesulfonic acid Chemical compound C=1C=CC=CC=1N(C)CC1=CC=CC(S(O)(=O)=O)=C1 JIYCNHGAAQDQTN-UHFFFAOYSA-N 0.000 description 2
- RXGJTUSBYWCRBK-UHFFFAOYSA-M 5-methylphenazinium methyl sulfate Chemical compound COS([O-])(=O)=O.C1=CC=C2[N+](C)=C(C=CC=C3)C3=NC2=C1 RXGJTUSBYWCRBK-UHFFFAOYSA-M 0.000 description 2
- IKHGUXGNUITLKF-UHFFFAOYSA-N Acetaldehyde Chemical compound CC=O IKHGUXGNUITLKF-UHFFFAOYSA-N 0.000 description 2
- 101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 description 2
- 101000928746 Homo sapiens Dehydrogenase/reductase SDR family member 9 Proteins 0.000 description 2
- JLTDJTHDQAWBAV-UHFFFAOYSA-N N,N-dimethylaniline Chemical compound CN(C)C1=CC=CC=C1 JLTDJTHDQAWBAV-UHFFFAOYSA-N 0.000 description 2
- UFWIBTONFRDIAS-UHFFFAOYSA-N Naphthalene Chemical class C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- MVPPADPHJFYWMZ-UHFFFAOYSA-N chlorobenzene Chemical compound ClC1=CC=CC=C1 MVPPADPHJFYWMZ-UHFFFAOYSA-N 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- RXKJFZQQPQGTFL-UHFFFAOYSA-N dihydroxyacetone Chemical compound OCC(=O)CO RXKJFZQQPQGTFL-UHFFFAOYSA-N 0.000 description 2
- 238000004817 gas chromatography Methods 0.000 description 2
- 125000005843 halogen group Chemical group 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000001630 malic acid Substances 0.000 description 2
- 235000011090 malic acid Nutrition 0.000 description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 2
- UHOVQNZJYSORNB-UHFFFAOYSA-N monobenzene Natural products C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 2
- ISGXOWLMGOPVPB-UHFFFAOYSA-N n,n-dibenzylaniline Chemical compound C=1C=CC=CC=1CN(C=1C=CC=CC=1)CC1=CC=CC=C1 ISGXOWLMGOPVPB-UHFFFAOYSA-N 0.000 description 2
- KHPXUQMNIQBQEV-UHFFFAOYSA-N oxaloacetic acid Chemical compound OC(=O)CC(=O)C(O)=O KHPXUQMNIQBQEV-UHFFFAOYSA-N 0.000 description 2
- 239000012279 sodium borohydride Substances 0.000 description 2
- 229910000033 sodium borohydride Inorganic materials 0.000 description 2
- NGSFWBMYFKHRBD-DKWTVANSSA-M sodium;(2s)-2-hydroxypropanoate Chemical compound [Na+].C[C@H](O)C([O-])=O NGSFWBMYFKHRBD-DKWTVANSSA-M 0.000 description 2
- 125000001424 substituent group Chemical group 0.000 description 2
- 125000003831 tetrazolyl group Chemical group 0.000 description 2
- HSINOMROUCMIEA-FGVHQWLLSA-N (2s,4r)-4-[(3r,5s,6r,7r,8s,9s,10s,13r,14s,17r)-6-ethyl-3,7-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methylpentanoic acid Chemical compound C([C@@]12C)C[C@@H](O)C[C@H]1[C@@H](CC)[C@@H](O)[C@@H]1[C@@H]2CC[C@]2(C)[C@@H]([C@H](C)C[C@H](C)C(O)=O)CC[C@H]21 HSINOMROUCMIEA-FGVHQWLLSA-N 0.000 description 1
- BUXHYMZMVMNDMG-UHFFFAOYSA-N 2,5-dichlorobenzaldehyde Chemical compound ClC1=CC=C(Cl)C(C=O)=C1 BUXHYMZMVMNDMG-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- JLFWTLNLOSUFMU-UHFFFAOYSA-N 2-methyl-5-nitrobenzaldehyde Chemical compound CC1=CC=C([N+]([O-])=O)C=C1C=O JLFWTLNLOSUFMU-UHFFFAOYSA-N 0.000 description 1
- KVZLHPXEUGJPAH-UHFFFAOYSA-N 2-oxidanylpropanoic acid Chemical compound CC(O)C(O)=O.CC(O)C(O)=O KVZLHPXEUGJPAH-UHFFFAOYSA-N 0.000 description 1
- KJHJAABRDASKAF-UHFFFAOYSA-N 3,7-dihydropurine-2,6-dione Chemical compound OC1=NC(O)=C2N=CNC2=N1.O=C1NC(=O)NC2=C1NC=N2 KJHJAABRDASKAF-UHFFFAOYSA-N 0.000 description 1
- 101710172561 3alpha-hydroxysteroid dehydrogenase Proteins 0.000 description 1
- IJOJIVNDFQSGAB-SQOUGZDYSA-N 6-O-phosphono-D-glucono-1,5-lactone Chemical compound O[C@H]1[C@H](O)[C@@H](COP(O)(O)=O)OC(=O)[C@@H]1O IJOJIVNDFQSGAB-SQOUGZDYSA-N 0.000 description 1
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- SXZYCXMUPBBULW-AIHAYLRMSA-N D-galactono-1,4-lactone Chemical compound OC[C@@H](O)[C@@H]1OC(=O)[C@H](O)[C@H]1O SXZYCXMUPBBULW-AIHAYLRMSA-N 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 102000013460 Malate Dehydrogenase Human genes 0.000 description 1
- 108010026217 Malate Dehydrogenase Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 229920004923 Triton X-15 Polymers 0.000 description 1
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 1
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 125000004453 alkoxycarbonyl group Chemical group 0.000 description 1
- 125000004466 alkoxycarbonylamino group Chemical group 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- 125000004103 aminoalkyl group Chemical group 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 150000001555 benzenes Chemical class 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- YLZSIUVOIFJGQZ-UHFFFAOYSA-N bis[4-(dimethylamino)phenyl]methanol Chemical compound C1=CC(N(C)C)=CC=C1C(O)C1=CC=C(N(C)C)C=C1 YLZSIUVOIFJGQZ-UHFFFAOYSA-N 0.000 description 1
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
- 229910052794 bromium Inorganic materials 0.000 description 1
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000004063 butyryl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 229940120503 dihydroxyacetone Drugs 0.000 description 1
- 125000002147 dimethylamino group Chemical group [H]C([H])([H])N(*)C([H])([H])[H] 0.000 description 1
- QILSFLSDHQAZET-UHFFFAOYSA-N diphenylmethanol Chemical compound C=1C=CC=CC=1C(O)C1=CC=CC=C1 QILSFLSDHQAZET-UHFFFAOYSA-N 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 125000001153 fluoro group Chemical group F* 0.000 description 1
- 108010090622 glycerol oxidase Proteins 0.000 description 1
- 239000000543 intermediate Substances 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229910000464 lead oxide Inorganic materials 0.000 description 1
- 229940049920 malate Drugs 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- NSTOKAMMRZTATB-UHFFFAOYSA-N n-butyl-2-methylaniline Chemical compound CCCCNC1=CC=CC=C1C NSTOKAMMRZTATB-UHFFFAOYSA-N 0.000 description 1
- 125000001624 naphthyl group Chemical group 0.000 description 1
- 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 description 1
- YEXPOXQUZXUXJW-UHFFFAOYSA-N oxolead Chemical compound [Pb]=O YEXPOXQUZXUXJW-UHFFFAOYSA-N 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 125000001147 pentyl group Chemical group C(CCCC)* 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000012802 pre-warming Methods 0.000 description 1
- 125000001501 propionyl group Chemical group O=C([*])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 238000006722 reduction reaction Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229940065721 systemic for obstructive airway disease xanthines Drugs 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 229940116269 uric acid Drugs 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 229940075420 xanthine Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
æ¬çºæã¯NAD(P)ãéå
åãã³ãã³ã¢ããã¢ã
ãã³ãžãã¯ã¬ãªããïŒãã¹ããšãŒãïŒãã®å®éæ¹
æ³åã³å®éçšçµæç©ã«é¢ããã åŸæ¥NAD(P)ã¯NAD(P)ã®çŽ«å€éšã«ããã
åžåãçŽæ¥æž¬å®ããæ¹æ³NAD(P)ããã€ã¢ã
ã©ãŒãŒãä»ããŠèå ç©è³ªã«å°ãããã®èå ã枬å®
ããæ¹æ³NAD(P)ããã€ã¢ãã©ãŒãŒãä»ããŠ
ããã©ãŸãªãŠã å¡©ãããã«ãã¶ã³ã«å°ããããã
æ¯è²å®éããæ¹æ³ãç¥ãããŠããã ãããã®æ¹æ³ã«ã¯ã以äžã®ãããªçæãããã
å³ã¡ãã«ã€ããŠã¯ãæ床ãäœããèšåºæ€æ»ã«å¿
çšããå Žåã玫å€éšã§ã®æž¬å®ã§ãããããè©Šæäž
ã®çäœæåã®åœ±é¿ãåãããããã«ã€ããŠã¯ã
èå å 床èšãå¿ èŠã§ãããã«ã€ããŠã¯ã埮éæ
åã®å®éã«éããŠæ床ãäœããçæãããã«ãã¶
ã³ãæ°Žã«é£æº¶ã§ãåšå ·çã«æ²çããèœã¡ã«ããã
ããã©ãŸãªãŠã å¡©ããã«ãã¶ã³ãšãã«å ã«äžå®å®
ãªãã®ãå€ããè©Šè¬ä¿åé¢ããã³æž¬å®æã«æ³šæã
èŠããã ãã粟床ããNAD(P)ãå®éããæ¹æ³ã®éçº
ãæãŸããŠããããã®ç®çã®ããæ€èšã®çµæåŸè¿°
ã®äžè¬åŒïŒïŒã«ç€ºãååç©ãšNAD(P)ãšãå
å¿ãããŠçæããè²çŽ ã¯æ¥µå€§åžåæ³¢é·ã600nïœ
ååŸã«ãããè©Šæäžç¹ã«çäœæåäžã®æåã®åœ±é¿
ãåãã«ããããšãå¯èŠéšã«ããã枬å®ãå¯èœã§
ããããšãæ床ãåªããŠãããè©Šæãå°éã§å®é
ã§ããããšã氎溶æ§ã§ããããåšå ·ãžã®æ²çãæ®
ã©ãªãããšçã®åªããæ§è³ªãæããããšãèŠåºã
ããã æ¬çºæã«ããã°NAD(P)ã¯ãã€ã¢ãã©ãŒãŒ
ãããã¯é»åãã€ãªã¢ãŒåã³ãã«ãªãã·ããŒãŒ
ãããã¯ããªãŒã«ãªãã·ããªãã¯ã¿ãŒãŒã®ååšäž
äžèšäžè¬åŒïŒïŒã§è¡šãããååç©ã以äžãåå
ç©ïŒïŒãšããããšåå¿ãããŠçæããè²çŽ ãå®
éããããšã«ããå®éãããã äžè¬åŒïŒïŒ åŒäžïŒ¹ã¯æ°ŽçŽ ãããã¯ããããã·ã«ã瀺ãã
R1ãR2ãR3ã¯åäžãããã¯ç°ãªã€ãŠãããäžèš
äžè¬åŒïŒïŒãïŒïŒåã¯ïŒïŒã§è¡šãããåºã瀺
ãã ã¯æ°ŽçŽ ãããããã·ã«ãã¢ããã眮æã¢ã
ããã¢ã«ãã«ã眮æã¢ã«ãã«ãã¢ã«ã±ãã«ãã¢ãª
ãŒã«ã眮æã¢ãªãŒã«ãã¢ã·ã«ãããã²ã³ååãã
ãããã¹ã«ããã«ã«ããã·ã«ãã¢ã«ã³ãã·ã瀺
ããïœã¯ïŒãïŒåã¯ïŒã瀺ããR1ãR2ãR3ã®å°
ãªããšãïŒã€ã®ïŒºã¯ããããã·ã«ãã¢ããåã¯çœ®
æã¢ããã§ãããåãZnã«ãããåã¯åäžã
ããã¯ç°ãªã€ãŠãããã äžèšåå®çŸ©ã«ãããŠã¢ã«ãã«ã¯ççŽ æ°ïŒãïŒã®
ã¢ã«ãã«ãå å«ããã¡ãã«ããšãã«ããããã«ã
ããã«ããã³ãã«çã瀺ããã¢ã«ã±ãã«ã¯ççŽ æ°
ïŒãïŒã®åºãå å«ããããã«ããããã¬ã³ããã
ã¬ã³çãäŸç€ºããããã¢ã«ã³ãã·ã¯ççŽ æ°ïŒãïŒ
ã®åºãå å«ããã¡ããã·ããšããã·ããããã
ã·ããããã·çãäŸç€ºãããã ã¢ãªãŒã«ãšããŠã¯ãããšãã«ããããã«ãäŸç€º
ãããã ã¢ã·ã«ã¯ççŽ æ°ïŒãïŒã®ã¢ã·ã«ãå å«ããã¢ã»
ãã«ãããããªãã«ããããªã«çãäŸç€ºãããã
ããã²ã³ã¯å¡©çŽ ååãèçŽ ååãããçŽ ååãå
å«ããã 眮æã¢ããã眮æã¢ã«ãã«ã眮æã¢ãªãŒã«ã«ã
ãã眮æåºãšããŠã¯ãã¢ã«ãã«ãã¢ã«ã±ãã«ãã¢
ãªãŒã«ãã¢ã«ã³ãã·ãããããã·ã«ãã«ã«ããã·
ã«ãã¹ã«ããã¹ã«ããã«ãããã²ã³ååãã¢ã
ããã¢ã«ã³ãã·ã«ã«ããã«ã¢ãããã¢ã«ã³ãã·ã«
ã«ããã«ãã¢ããã¢ã«ãã«ãã¢ã·ã«ããããçã
äŸç€ºãããã 該眮æåºäžã®ã¢ã«ãã«ãã¢ã«ã±ãã«ãã¢ãªãŒ
ã«ãã¢ã«ã³ãã·ãããã²ã³ãã¢ããçã¯åèšãšå
矩ãæããã æ¬çºæãå®æœããã«éããŠã¯äžè¬ã«ç·©è¡æ¶²äŸã
ã°ã°ãããããã¢ãŒããªã³é žãããã¢ãŒãããŠé ž
ãããã¢ãŒãé ¢é žãããã¢ãŒãããªã¹ãããã¢ãŒ
çã«ãã€ã¢ãã©ãŒãŒåã¯é»åãã€ãªã€ãŒäŸãã°ã
ãšããžã³ã¡ããµã«ããšãŒãïŒPMSïŒãïŒâã¡ãã
ã·ããšããžã³ã¡ããµã«ããšãŒãïŒMPMSïŒãã¡ã«
ãã©ãã«ãŒçåã³ããŒãªãã·ããŒãŒåã¯ããªãŒã«
ãªãã·ããªãã¯ã¿ãŒãŒåã³ååç©ïŒïŒãå ããŠ
è©Šè¬æ¶²ãšããã è©Šæã«è©Šè¬æ¶²ãå ã30ã50âã®é žçŽ ã倱掻ããª
ã枩床ã§åå¿ãããçæããè²çŽ ã«ãã€ãŠçè²ã
ãåå¿æ¶²ã®åžåãè²çŽ ã®æ¥µå€§åžåæ³¢é·ã§è©Šè¬ãã©
ã³ã¯ãå¯Ÿç §ãšããŠæž¬å®ãããããããæ¢ç¥éã«ã€
ããŠã®ãã¹ãããæ±ããæ€éç·ããè©Šæäžã®
NAD(P)ãå®éããããšãã§ããã æ¬çºææ¹æ³ã¯åŸè¿°ã®åŠãåå¿ã«ãã€ãŠNAD(P)
ãçæããåå¿ç³»ã«ä¿ãåå¿ç©è³ªãããã¯é µçŽ
掻æ§ãå®éããæ¹æ³ã«é©çšã§ãããå®éãããã¹
ãå åãååç©ã§ããå Žåã«ã¯äžè¬ã«äžèšåžå枬
å®ã«éããŠåå¿ãçŽïŒã10åè¡ãããåŸåå¿æ¶²ã®
åžåãæ¯è²ã«ãã€ãŠæž¬å®ããããšã«ãã€ãŠç®çå
åãå®éã§ãããå åãé µçŽ æŽ»æ§ã§ããå Žåã«ã¯
äžè¬ã«åå¿éå§åŸã®é©åœãªæéã«ãããè²çŽ çæ
é床ãåå¿æ¶²ã®åžå 床ã®å€åããæ±ããããšã«ã
ã€ãŠè©²æŽ»æ§ãå®éã§ããã ç·©è¡æ¶²ã¯10ïœïŒã1Mã®æ¿åºŠã§çšãããããã
ã«ãªãã·ããŒãŒãããªãŒã«ãªãã·ããªãã¯ã¿ãŒ
ãŒããã€ã¢ãã©ãŒãŒã¯ïŒã500UïŒmlã§é»åãã€
ãªã€ãŒã¯0.0001ã0.01mgïŒmlã§çšãããããåå
ç©ïŒïŒã¯åå¿ããNAD(P)ãšçã¢ã«ä»¥äžãé
åžž0.001ãïŒmgïŒmlã§çšããããã åå¿ã«éããŠNAD(P)ã¯éåžžè©Šæäžã®æ¿åºŠã
0.001ãïŒmgïŒmlãšãªãæ§ã«è©Šè¬æ¶²ãããã¯èžç
æ°Žã§èª¿æŽãããã NAD(P)ãçæããåå¿ç³»ã§æ¬çºæãé©çšã
ããå Žåã«ã¯ãåå¿ã«é¢äžããåºè³ªã®ãããªåå¿
ç©è³ªãé µçŽ ãNADçã¯ã枬å®ãã¹ã察象ã§ãã
ãåŠãã«ãã€ãŠçšããããéãç°ãªãå Žåãå€ã
ããäžè¬ã«ãããã0.0001ã100mgïŒmlã10ã
1000UïŒmlã0.0001ã100mgïŒmlã§çšããããã åãå¿ èŠã«å¿ããŠTriton â100çã®çé¢æŽ»
æ§å€ãçšããããã ãã€ã€ãã©ãŒãŒä»¥å€ã®é»åãã€ãªã¢ãŒãçšãã
ããå Žåãé»åãã€ãªã€ãŒã®ãã€ã€ãã©ãŒãŒã«å¯Ÿ
ããçžå¯ŸæŽ»æ§ã¯äžèšã®ãšããã§ãããããããã
ææšãšããŠçšããã°åã ã«æ€éç·ãæ±ããããšãª
ãå®æœã§ããã ïŒæž¬å®æ¹æ³ïŒ 100ïœïŒ Goodã®ç·©è¡æ¶²ïŒPH8.0ïŒã«ãã«ãªã
ã·ããŒãŒ10UïŒmlãTriton â100 0.1ïŒ ãåå
ç©No.41ã0.1mgïŒmlãNADH 50ïœïŒïŒã溶解
ããè©Šè¬æ¶²ãšããã 37âã«ä¿ã€ãäžå®éã®è©Šè¬æ¶²ã«å¯Ÿãããã€ã¢
ãã©ãŒãŒ5UïŒmlPMS0.001mgïŒml
MPMS0.001mgïŒmlã¡ã«ãã©ãã«ãŒ0.001mgïŒml
ããã®æ¿åºŠãšãªãããã«ãããã溶解ããæ£ç¢ºãª
äžå®æéå ã®åžå 床å€åéã633nïœã«ãŠæž¬å®ã
ãããã®åžå 床å€åéããããã€ã¢ãã©ãŒãŒã
100ãšããæã®çžå¯ŸæŽ»æ§ãç®åºããã
ãã³ãžãã¯ã¬ãªããïŒãã¹ããšãŒãïŒãã®å®éæ¹
æ³åã³å®éçšçµæç©ã«é¢ããã åŸæ¥NAD(P)ã¯NAD(P)ã®çŽ«å€éšã«ããã
åžåãçŽæ¥æž¬å®ããæ¹æ³NAD(P)ããã€ã¢ã
ã©ãŒãŒãä»ããŠèå ç©è³ªã«å°ãããã®èå ã枬å®
ããæ¹æ³NAD(P)ããã€ã¢ãã©ãŒãŒãä»ããŠ
ããã©ãŸãªãŠã å¡©ãããã«ãã¶ã³ã«å°ããããã
æ¯è²å®éããæ¹æ³ãç¥ãããŠããã ãããã®æ¹æ³ã«ã¯ã以äžã®ãããªçæãããã
å³ã¡ãã«ã€ããŠã¯ãæ床ãäœããèšåºæ€æ»ã«å¿
çšããå Žåã玫å€éšã§ã®æž¬å®ã§ãããããè©Šæäž
ã®çäœæåã®åœ±é¿ãåãããããã«ã€ããŠã¯ã
èå å 床èšãå¿ èŠã§ãããã«ã€ããŠã¯ã埮éæ
åã®å®éã«éããŠæ床ãäœããçæãããã«ãã¶
ã³ãæ°Žã«é£æº¶ã§ãåšå ·çã«æ²çããèœã¡ã«ããã
ããã©ãŸãªãŠã å¡©ããã«ãã¶ã³ãšãã«å ã«äžå®å®
ãªãã®ãå€ããè©Šè¬ä¿åé¢ããã³æž¬å®æã«æ³šæã
èŠããã ãã粟床ããNAD(P)ãå®éããæ¹æ³ã®éçº
ãæãŸããŠããããã®ç®çã®ããæ€èšã®çµæåŸè¿°
ã®äžè¬åŒïŒïŒã«ç€ºãååç©ãšNAD(P)ãšãå
å¿ãããŠçæããè²çŽ ã¯æ¥µå€§åžåæ³¢é·ã600nïœ
ååŸã«ãããè©Šæäžç¹ã«çäœæåäžã®æåã®åœ±é¿
ãåãã«ããããšãå¯èŠéšã«ããã枬å®ãå¯èœã§
ããããšãæ床ãåªããŠãããè©Šæãå°éã§å®é
ã§ããããšã氎溶æ§ã§ããããåšå ·ãžã®æ²çãæ®
ã©ãªãããšçã®åªããæ§è³ªãæããããšãèŠåºã
ããã æ¬çºæã«ããã°NAD(P)ã¯ãã€ã¢ãã©ãŒãŒ
ãããã¯é»åãã€ãªã¢ãŒåã³ãã«ãªãã·ããŒãŒ
ãããã¯ããªãŒã«ãªãã·ããªãã¯ã¿ãŒãŒã®ååšäž
äžèšäžè¬åŒïŒïŒã§è¡šãããååç©ã以äžãåå
ç©ïŒïŒãšããããšåå¿ãããŠçæããè²çŽ ãå®
éããããšã«ããå®éãããã äžè¬åŒïŒïŒ åŒäžïŒ¹ã¯æ°ŽçŽ ãããã¯ããããã·ã«ã瀺ãã
R1ãR2ãR3ã¯åäžãããã¯ç°ãªã€ãŠãããäžèš
äžè¬åŒïŒïŒãïŒïŒåã¯ïŒïŒã§è¡šãããåºã瀺
ãã ã¯æ°ŽçŽ ãããããã·ã«ãã¢ããã眮æã¢ã
ããã¢ã«ãã«ã眮æã¢ã«ãã«ãã¢ã«ã±ãã«ãã¢ãª
ãŒã«ã眮æã¢ãªãŒã«ãã¢ã·ã«ãããã²ã³ååãã
ãããã¹ã«ããã«ã«ããã·ã«ãã¢ã«ã³ãã·ã瀺
ããïœã¯ïŒãïŒåã¯ïŒã瀺ããR1ãR2ãR3ã®å°
ãªããšãïŒã€ã®ïŒºã¯ããããã·ã«ãã¢ããåã¯çœ®
æã¢ããã§ãããåãZnã«ãããåã¯åäžã
ããã¯ç°ãªã€ãŠãããã äžèšåå®çŸ©ã«ãããŠã¢ã«ãã«ã¯ççŽ æ°ïŒãïŒã®
ã¢ã«ãã«ãå å«ããã¡ãã«ããšãã«ããããã«ã
ããã«ããã³ãã«çã瀺ããã¢ã«ã±ãã«ã¯ççŽ æ°
ïŒãïŒã®åºãå å«ããããã«ããããã¬ã³ããã
ã¬ã³çãäŸç€ºããããã¢ã«ã³ãã·ã¯ççŽ æ°ïŒãïŒ
ã®åºãå å«ããã¡ããã·ããšããã·ããããã
ã·ããããã·çãäŸç€ºãããã ã¢ãªãŒã«ãšããŠã¯ãããšãã«ããããã«ãäŸç€º
ãããã ã¢ã·ã«ã¯ççŽ æ°ïŒãïŒã®ã¢ã·ã«ãå å«ããã¢ã»
ãã«ãããããªãã«ããããªã«çãäŸç€ºãããã
ããã²ã³ã¯å¡©çŽ ååãèçŽ ååãããçŽ ååãå
å«ããã 眮æã¢ããã眮æã¢ã«ãã«ã眮æã¢ãªãŒã«ã«ã
ãã眮æåºãšããŠã¯ãã¢ã«ãã«ãã¢ã«ã±ãã«ãã¢
ãªãŒã«ãã¢ã«ã³ãã·ãããããã·ã«ãã«ã«ããã·
ã«ãã¹ã«ããã¹ã«ããã«ãããã²ã³ååãã¢ã
ããã¢ã«ã³ãã·ã«ã«ããã«ã¢ãããã¢ã«ã³ãã·ã«
ã«ããã«ãã¢ããã¢ã«ãã«ãã¢ã·ã«ããããçã
äŸç€ºãããã 該眮æåºäžã®ã¢ã«ãã«ãã¢ã«ã±ãã«ãã¢ãªãŒ
ã«ãã¢ã«ã³ãã·ãããã²ã³ãã¢ããçã¯åèšãšå
矩ãæããã æ¬çºæãå®æœããã«éããŠã¯äžè¬ã«ç·©è¡æ¶²äŸã
ã°ã°ãããããã¢ãŒããªã³é žãããã¢ãŒãããŠé ž
ãããã¢ãŒãé ¢é žãããã¢ãŒãããªã¹ãããã¢ãŒ
çã«ãã€ã¢ãã©ãŒãŒåã¯é»åãã€ãªã€ãŒäŸãã°ã
ãšããžã³ã¡ããµã«ããšãŒãïŒPMSïŒãïŒâã¡ãã
ã·ããšããžã³ã¡ããµã«ããšãŒãïŒMPMSïŒãã¡ã«
ãã©ãã«ãŒçåã³ããŒãªãã·ããŒãŒåã¯ããªãŒã«
ãªãã·ããªãã¯ã¿ãŒãŒåã³ååç©ïŒïŒãå ããŠ
è©Šè¬æ¶²ãšããã è©Šæã«è©Šè¬æ¶²ãå ã30ã50âã®é žçŽ ã倱掻ããª
ã枩床ã§åå¿ãããçæããè²çŽ ã«ãã€ãŠçè²ã
ãåå¿æ¶²ã®åžåãè²çŽ ã®æ¥µå€§åžåæ³¢é·ã§è©Šè¬ãã©
ã³ã¯ãå¯Ÿç §ãšããŠæž¬å®ãããããããæ¢ç¥éã«ã€
ããŠã®ãã¹ãããæ±ããæ€éç·ããè©Šæäžã®
NAD(P)ãå®éããããšãã§ããã æ¬çºææ¹æ³ã¯åŸè¿°ã®åŠãåå¿ã«ãã€ãŠNAD(P)
ãçæããåå¿ç³»ã«ä¿ãåå¿ç©è³ªãããã¯é µçŽ
掻æ§ãå®éããæ¹æ³ã«é©çšã§ãããå®éãããã¹
ãå åãååç©ã§ããå Žåã«ã¯äžè¬ã«äžèšåžå枬
å®ã«éããŠåå¿ãçŽïŒã10åè¡ãããåŸåå¿æ¶²ã®
åžåãæ¯è²ã«ãã€ãŠæž¬å®ããããšã«ãã€ãŠç®çå
åãå®éã§ãããå åãé µçŽ æŽ»æ§ã§ããå Žåã«ã¯
äžè¬ã«åå¿éå§åŸã®é©åœãªæéã«ãããè²çŽ çæ
é床ãåå¿æ¶²ã®åžå 床ã®å€åããæ±ããããšã«ã
ã€ãŠè©²æŽ»æ§ãå®éã§ããã ç·©è¡æ¶²ã¯10ïœïŒã1Mã®æ¿åºŠã§çšãããããã
ã«ãªãã·ããŒãŒãããªãŒã«ãªãã·ããªãã¯ã¿ãŒ
ãŒããã€ã¢ãã©ãŒãŒã¯ïŒã500UïŒmlã§é»åãã€
ãªã€ãŒã¯0.0001ã0.01mgïŒmlã§çšãããããåå
ç©ïŒïŒã¯åå¿ããNAD(P)ãšçã¢ã«ä»¥äžãé
åžž0.001ãïŒmgïŒmlã§çšããããã åå¿ã«éããŠNAD(P)ã¯éåžžè©Šæäžã®æ¿åºŠã
0.001ãïŒmgïŒmlãšãªãæ§ã«è©Šè¬æ¶²ãããã¯èžç
æ°Žã§èª¿æŽãããã NAD(P)ãçæããåå¿ç³»ã§æ¬çºæãé©çšã
ããå Žåã«ã¯ãåå¿ã«é¢äžããåºè³ªã®ãããªåå¿
ç©è³ªãé µçŽ ãNADçã¯ã枬å®ãã¹ã察象ã§ãã
ãåŠãã«ãã€ãŠçšããããéãç°ãªãå Žåãå€ã
ããäžè¬ã«ãããã0.0001ã100mgïŒmlã10ã
1000UïŒmlã0.0001ã100mgïŒmlã§çšããããã åãå¿ èŠã«å¿ããŠTriton â100çã®çé¢æŽ»
æ§å€ãçšããããã ãã€ã€ãã©ãŒãŒä»¥å€ã®é»åãã€ãªã¢ãŒãçšãã
ããå Žåãé»åãã€ãªã€ãŒã®ãã€ã€ãã©ãŒãŒã«å¯Ÿ
ããçžå¯ŸæŽ»æ§ã¯äžèšã®ãšããã§ãããããããã
ææšãšããŠçšããã°åã ã«æ€éç·ãæ±ããããšãª
ãå®æœã§ããã ïŒæž¬å®æ¹æ³ïŒ 100ïœïŒ Goodã®ç·©è¡æ¶²ïŒPH8.0ïŒã«ãã«ãªã
ã·ããŒãŒ10UïŒmlãTriton â100 0.1ïŒ ãåå
ç©No.41ã0.1mgïŒmlãNADH 50ïœïŒïŒã溶解
ããè©Šè¬æ¶²ãšããã 37âã«ä¿ã€ãäžå®éã®è©Šè¬æ¶²ã«å¯Ÿãããã€ã¢
ãã©ãŒãŒ5UïŒmlPMS0.001mgïŒml
MPMS0.001mgïŒmlã¡ã«ãã©ãã«ãŒ0.001mgïŒml
ããã®æ¿åºŠãšãªãããã«ãããã溶解ããæ£ç¢ºãª
äžå®æéå ã®åžå 床å€åéã633nïœã«ãŠæž¬å®ã
ãããã®åžå 床å€åéããããã€ã¢ãã©ãŒãŒã
100ãšããæã®çžå¯ŸæŽ»æ§ãç®åºããã
ãè¡šã
æ¬çºæã§çšããããååç©ïŒïŒã®å
·äœäŸãšã
ã®æ¥µå€§åžåæ³¢é·ïŒÎ»maxïŒãæ床ãæ°Žãžã®æº¶è§£æ§
ã第ïŒãïŒè¡šã«ç€ºãããã è¡šäžã®ç¥å·ã¯äžèšåºã瀺ããå第ïŒãïŒè¡šã«ã
ããŠâå ã¯äœçœ®ã瀺ããåºã®è¡šç€ºã®ãªãäœçœ®ã¯ã
ãããæ°ŽçŽ ã瀺ãã æ床ã®æž¬å®ã¯æ¬¡ã®æ¹æ³ã«ããè¡ãããã NADHæ¶²ïŒ 0.1Mããªã¹âHClç·©è¡æ¶²ïŒPH8.0ïŒã«NADHã
å ããŠ0.5ïœïŒïŒã®æº¶æ¶²ãšããã è©Šè¬æ¶²ïŒ Goodç·©è¡æ¶²ã«10UïŒmlãã«ãªãã·ããŒãŒã
5UïŒmlãã€ã¢ãã©ãŒãŒã0.1mgïŒmlããªãã³ïŒžâ
100åã³0.1mgïŒmlååç©ïŒïŒïŒåã¯0.01mgïŒml
ã®ãããããã©ãŸãªãŠã ãã«ãŒïŒãå«æãã溶液
ã調補ããã 50ÎŒã®NADH液ã«ïŒmlã®è©Šè¬æ¶²ãå ããŠåå¿
ããλmaxã«ãããODå€ã枬å®ããNTãçšãã
å Žåã100ãšãããšãã®çžå¯Ÿå€ã瀺ãã 溶解æ§ã¯20âã®èžçæ°Žã«å¯Ÿãã溶解床ã0.05
mgïŒml以äžãã0.2mgïŒml以äžãAAãšããŠè¡šã
ããã
ã®æ¥µå€§åžåæ³¢é·ïŒÎ»maxïŒãæ床ãæ°Žãžã®æº¶è§£æ§
ã第ïŒãïŒè¡šã«ç€ºãããã è¡šäžã®ç¥å·ã¯äžèšåºã瀺ããå第ïŒãïŒè¡šã«ã
ããŠâå ã¯äœçœ®ã瀺ããåºã®è¡šç€ºã®ãªãäœçœ®ã¯ã
ãããæ°ŽçŽ ã瀺ãã æ床ã®æž¬å®ã¯æ¬¡ã®æ¹æ³ã«ããè¡ãããã NADHæ¶²ïŒ 0.1Mããªã¹âHClç·©è¡æ¶²ïŒPH8.0ïŒã«NADHã
å ããŠ0.5ïœïŒïŒã®æº¶æ¶²ãšããã è©Šè¬æ¶²ïŒ Goodç·©è¡æ¶²ã«10UïŒmlãã«ãªãã·ããŒãŒã
5UïŒmlãã€ã¢ãã©ãŒãŒã0.1mgïŒmlããªãã³ïŒžâ
100åã³0.1mgïŒmlååç©ïŒïŒïŒåã¯0.01mgïŒml
ã®ãããããã©ãŸãªãŠã ãã«ãŒïŒãå«æãã溶液
ã調補ããã 50ÎŒã®NADH液ã«ïŒmlã®è©Šè¬æ¶²ãå ããŠåå¿
ããλmaxã«ãããODå€ã枬å®ããNTãçšãã
å Žåã100ãšãããšãã®çžå¯Ÿå€ã瀺ãã 溶解æ§ã¯20âã®èžçæ°Žã«å¯Ÿãã溶解床ã0.05
mgïŒml以äžãã0.2mgïŒml以äžãAAãšããŠè¡šã
ããã
ãè¡šã
ãè¡šã
ãè¡šã
ãè¡šã
ãè¡šã
ãè¡šã
ãè¡šã
ãè¡šã
ãè¡šã
ãè¡šã
ãŒ
æ¬çºæã§çšããããååç©ïŒïŒã¯ææåæã®
äžéäœãšããŠå ¬ç¥ã®ååç©ã§ããã第ïŒãïŒè¡šã«
èšèŒã®ååç©ã¯äŸãã°ä»¥äžã®æ¹æ³ã«ãã€ãŠåæã§
ããã äžèšè¡šã«èšèŒã®ååç©No.ã®ååç©ã«ã€ããŠã¯
ïŒã4â²âãã¹ïŒãžã¡ãã«ã¢ããïŒãã³ãºããã
ãŒã«ãšç®çååç©ã«ãããäžèšã«ç€ºããã眮æ
ãã³ãŒã³ãããã¯çœ®æããã¿ã¬ã³ãšãçã¢ã«ã¥
ã€çŽåæã®ïŒã10åéã®60ïŒ ç¡«é žäžã§åå¿ãã
ããåå¿ã¯50ã100âã§æ¹æããŠè¡ããïŒãïŒ
æéã§å®äºãããããã«åå¿æ¶²ã®ïŒãïŒåã®ïŒ
âååŸã®å·æ°Žãå ããŠäžå€æ¹æãç¶ããã åå¿ç©ãæ¿ŸéããïŒïŒ ç¡«é žã§æŽæµåŸç空也ç¥
ããŠç®çç©ãåŸããã¢ã«ã«ãªäŸãã°æ°Žé žåãã
ãªãŠã ãåå¿æ¶²ã«å ããããšã«ãã€ãŠæ²æŸ±ãç
æãæããªãã®ã§å¿ èŠã«å¿ããŠå ããã A1ïŒ A2ïŒ
æ¬çºæã§çšããããååç©ïŒïŒã¯ææåæã®
äžéäœãšããŠå ¬ç¥ã®ååç©ã§ããã第ïŒãïŒè¡šã«
èšèŒã®ååç©ã¯äŸãã°ä»¥äžã®æ¹æ³ã«ãã€ãŠåæã§
ããã äžèšè¡šã«èšèŒã®ååç©No.ã®ååç©ã«ã€ããŠã¯
ïŒã4â²âãã¹ïŒãžã¡ãã«ã¢ããïŒãã³ãºããã
ãŒã«ãšç®çååç©ã«ãããäžèšã«ç€ºããã眮æ
ãã³ãŒã³ãããã¯çœ®æããã¿ã¬ã³ãšãçã¢ã«ã¥
ã€çŽåæã®ïŒã10åéã®60ïŒ ç¡«é žäžã§åå¿ãã
ããåå¿ã¯50ã100âã§æ¹æããŠè¡ããïŒãïŒ
æéã§å®äºãããããã«åå¿æ¶²ã®ïŒãïŒåã®ïŒ
âååŸã®å·æ°Žãå ããŠäžå€æ¹æãç¶ããã åå¿ç©ãæ¿ŸéããïŒïŒ ç¡«é žã§æŽæµåŸç空也ç¥
ããŠç®çç©ãåŸããã¢ã«ã«ãªäŸãã°æ°Žé žåãã
ãªãŠã ãåå¿æ¶²ã«å ããããšã«ãã€ãŠæ²æŸ±ãç
æãæããªãã®ã§å¿ èŠã«å¿ããŠå ããã A1ïŒ A2ïŒ
ãè¡šã
ãè¡šã
äžèšä»¥å€ã®äœçœ®ã¯ããããæ°ŽçŽ ã瀺ãã
äžèšè¡šã«æ²ããåååç©No.ã«å¯Ÿå¿ããå
¬ç¥ã®
è²çŽ ãåæãšããåæè²çŽ ãè²çŽ ééã®80åé
ã®èžçæ°Žã§æº¶è§£ããããã®è²çŽ 溶液ãæ¹æããª
ãããåæè²çŽ ã®ïŒåmoléã®æ°ŽçŽ åããŠçŽ ã
ããªãŠã ãåŸã ã«æ·»å ããéå åå¿ãè¡ãã宀
æž©ã§çŽïŒæéæ¹æåŸããã®åå¿æ¶²ãããŒã¿ãªãŒ
ãšããã¬ãŒã¿ãŒã§æ¿çž®ä¹Ÿåºããã次ã«ãã®ä¹Ÿåº
ç©ã溶解ãããæäœå¿ èŠéã®èžçæ°Žã«ãŠæº¶è§£ã
ãããã®æº¶è§£æ¶²ã®15ã20å容éã®ã¬ãžã³HPâ
20ãã¬ã©ã¹ã«ã©ã ã«å å¡«ããã³ã³ãã€ã·ãšãã³
ã°åŸãåè¿°ã®æº¶è§£æ¶²ãå å¡«ãã䜿çšã¬ãžã³ã®ïŒ
ãïŒå容éã®èžçæ°Žãæµããæ®åããæ°ŽçŽ åã
ãŠçŽ ãããªãŠã ãé€å»ããããã®æç®çç©ã¯
HPâ20ã«åžçãããŠããã次ã«ã¡ã¿ããŒã«ïŒ
èžçæ°ŽïŒ1/1ã®å±é溶åªãæµãã溶åºæ¶²ãé©é
ã¥ã€ååããã溶åºæ¶²äžã®ç®çç©ãUVã¢ãã¿
ãŒãããã¯TLCã«ãŠç¢ºèªåŸãå¿ èŠãªãã©ã¯ã·
ãšã³ãéããããŒã¿ãªãŒãšããã¬ãŒã¿ãŒã§æ¿çž®
也åºããç®çç©ãåŸãã
è²çŽ ãåæãšããåæè²çŽ ãè²çŽ ééã®80åé
ã®èžçæ°Žã§æº¶è§£ããããã®è²çŽ 溶液ãæ¹æããª
ãããåæè²çŽ ã®ïŒåmoléã®æ°ŽçŽ åããŠçŽ ã
ããªãŠã ãåŸã ã«æ·»å ããéå åå¿ãè¡ãã宀
æž©ã§çŽïŒæéæ¹æåŸããã®åå¿æ¶²ãããŒã¿ãªãŒ
ãšããã¬ãŒã¿ãŒã§æ¿çž®ä¹Ÿåºããã次ã«ãã®ä¹Ÿåº
ç©ã溶解ãããæäœå¿ èŠéã®èžçæ°Žã«ãŠæº¶è§£ã
ãããã®æº¶è§£æ¶²ã®15ã20å容éã®ã¬ãžã³HPâ
20ãã¬ã©ã¹ã«ã©ã ã«å å¡«ããã³ã³ãã€ã·ãšãã³
ã°åŸãåè¿°ã®æº¶è§£æ¶²ãå å¡«ãã䜿çšã¬ãžã³ã®ïŒ
ãïŒå容éã®èžçæ°Žãæµããæ®åããæ°ŽçŽ åã
ãŠçŽ ãããªãŠã ãé€å»ããããã®æç®çç©ã¯
HPâ20ã«åžçãããŠããã次ã«ã¡ã¿ããŒã«ïŒ
èžçæ°ŽïŒ1/1ã®å±é溶åªãæµãã溶åºæ¶²ãé©é
ã¥ã€ååããã溶åºæ¶²äžã®ç®çç©ãUVã¢ãã¿
ãŒãããã¯TLCã«ãŠç¢ºèªåŸãå¿ èŠãªãã©ã¯ã·
ãšã³ãéããããŒã¿ãªãŒãšããã¬ãŒã¿ãŒã§æ¿çž®
也åºããç®çç©ãåŸãã
ãè¡šã
ååç©No.ïŒã10ã67ã68ã®è£œæ³
4.4â²âãã¹ïŒãžã¡ãã«ã¢ããïŒãã³ãºããã
ãŒã«ãšã¯ãã«ãã³ãŒã³ïŒååç©No.ïŒã10ïŒãã
ãã¯ïŒâããããŒã«â3.5âãžã¹ã«ãã³é žïŒå
åç©No.67.68ïŒãšã60ïŒ ç¡«é žãçšããŠåè¿°ã®
æ¹æ³ã«ãŠçž®åããããåŸãããååç©ãé©éã®
é ¢é žã«æº¶è§£ããé žåéã觊åªãšããŠããžã¡ãã«
ã¢ããåºã®ïŒã€ã®ã¡ãã«åºã®äžæ¹ãè±é¢ããã
ç®çç©ãåŸãã ååç©No.11ã12ã®è£œæ³ ïŒâã¡ãã«âïŒâããããã³ãºã¢ã«ããã€ã
ãšÎ±âïŒïŒ®âã¡ãã«ã¢ããªãïŒâïœâãã«ãšã³ã¹
ã«ãã³é žãšãã¢ã«æ¯ïŒïŒïŒã§åæååç©ç·éé
ã®10åéã®ç¡«é žã«å ãã100âååŸã§120æéæ¹
æãããæ²æŸ±ãç±ããŸãŸã°ã©ã¹ãã€ã«ã¿ãŒã«ãŠ
æ¿ŸéããïŒïŒ ç¡«é žã§æ¿ŸæŽåŸç空也ç¥ãè¡ãç®ç
ç©ãåŸãã ååç©No.19ã20ã®è£œæ³ ïŒâããããã·ãã³ãºã¢ã«ããã€ããšïŒ®ã
âãžã¡ãã«ã¢ããªã³ããã³ïŒ®âããšãã«âãžã
ã³ãžã«ã¢ãã³ãšãã¢ã«æ¯ïŒïŒïŒïŒïŒã§åæåå
ç©ç·ééã®10åéã®80ïŒ ç¡«é žã«å ãã190âå
åŸã§120æéæ¹æãããæ²æ®¿ãç±ããŸãŸã°ã©ã¹
ãã€ã«ã¿ãŒã«ãŠæ¿ŸéããïŒïŒ ç¡«é žã§æ¿ŸæŽåŸç空
也ç¥ãè¡ããç®çç©ãåŸãã ååç©No.21ã22ã®è£œæ³ ïŒãïŒâãžã¯ãããã³ãºã¢ã«ããã€ããšÎ±â
ïŒïŒ®âã¡ãã«ã¢ããªãïŒâïœâãã«ãšã³ã¹ã«ãã³
é žãšãã¢ã«æ¯ïŒïŒïŒã§åæååç©ç·ééã®10å
éã®ç¡«é žã«å ãã100âååŸã§120æéæ¹æã
ããæ²æ®¿ãç±ããŸãŸã°ã©ã¹ãã€ã«ã¿ãŒã«ãŠæ¿Ÿé
ããïŒïŒ ç¡«é žã§æ¿ŸæŽåŸç空也ç¥ãè¡ããç®çç©
ãåŸãã ååç©No.23ã24ã25ã26ã®è£œæ³ ïŒâããããã·ãã³ãºã¢ã«ããã€ããšïŒ®âã
ãšãã«ãžãã³ãžã«ã¢ãã³ïŒNo.23ã24ïŒãããã¯
âããã«ââãã«ã€ãžã³ïŒNo.25ã26ïŒãšã
ã¢ã«æ¯ïŒïŒïŒã§åæååç©ç·ééã®10åéã®ç¡«
é žã«å ãã100âååŸã§120æéæ¹æãããæ²æ®¿
ãç±ããŸãŸã°ã©ã¹ãã€ã«ã¿ãŒã«ãŠæ¿ŸéããïŒïŒ
ç¡«é žã§æ¿ŸæŽåŸç空也ç¥ãè¡ãç®çç©ãåŸãã äžèšä»¥å€ã®ååç©ã«ã€ããŠããäžèšã®æ¹æ³ã«ã
ããŠåæãç®çååç©ã«å¿ããŠéžã¶ããšã«ãã€ãŠ
ãããã¯å ¬ç¥æç®äŸãã°Color Index4å·»ã«èšèŒ
ã®æ¹æ³ã«ãã€ãŠè£œé ã§ããã æ¬çºææ¹æ³ã¯ãåå¿ã«ãã€ãŠNAD(P)ãååŠ
éè«çã«çæããåå¿ç³»ã«ãããåå¿ç©è³ªããã
ã¯é µçŽ 掻æ§ã®æž¬å®ã«é©çšã§ããããããåå¿ç³»ã¯
NADåã³ããããã²ããŒãŒãçšããå€ãã®åå¿
ç³»ãç¥ãããŠããããããç©è³ªãšããŠã°ã«ã³ãŒ
ã¹ãã¬ã©ã¯ããŒã¹ä¹³é žãã¢ã«ã³ãŒã«ããªã³ãŽé žã
ã¢ã«ããããããµã³ãã³ãã³ã¬ã¹ãããŒã«ãèæ±
é žãã©ã¯ããŒãããããã²ããŒãŒïŒLDHïŒæŽ»æ§ã
äžæ§èèªçããããããã ãããã®åå¿ç³»ãå³åŒçã«æ¬¡ã«ç€ºãããåå¿ç³»
ã«ãããåºè³ªãé µçŽ æŽ»æ§ã枬å®ã§ããã ïŒ ã°ã«ã³ãŒã¹ä» ã°ã«ã³ãŒã¹ïŒATPãããœãããŒãŒ âââââââââ ã°ã«ã³ãŒã¹âïŒâãªã³é žïŒADP ã°ã«ã³ãŒã¹âïŒâãªã³é žïŒNAD+ã°ã«ã³ãŒ
ã¹âïŒâ ãªã³é žè±æ°ŽçŽ é µçŽ ââââââââââ âã°ã«ã³ãâÎŽâã©ã¯ãã³ïŒâãªã³é žïŒHA
DHïŒN+ ïŒ ã¬ã©ã¯ããŒã¹ ã¬ã©ã¯ããŒã¹ïŒNAD+ã¬ã©ã¯ããŒã¹ããããã²ããŒ
㌠âââââââââââââââââ âã¬ã©ã¯ããâγâã©ã¯ãã³ïŒNADHïŒ
H+ ïŒ ä¹³é ž ä¹³é žïŒNADã©ã¯ããŒãã»ããããã²ããŒãŒïŒLDHïŒ âââââââââââââââââââââââ ãã«ãã³é žïŒNADHïŒH+ ïŒ ã¢ã«ã³ãŒã« ã¢ã«ã³ãŒã«ïŒãšã¿ããŒã«ïŒïŒNAD+ã¢ã«ã³ãŒã«
ããããã²ããŒãŒ ââââââââââââââââ ã¢ã«ãããïŒã¢ã»ãã¢ã«ãããïŒïŒNADHïŒH+ ïŒ ãªã³ãŽé ž ãªã³ãŽé žïŒNAD+ãã©ãŒãããããã²ããŒãŒ ââââââââââââââââ ãªãã¶ã«é ¢é žïŒNADHïŒH+ ïŒ ã¢ã«ããã âCHOïŒNAD+ïŒH2Oã¢ã«ãããããããã²ããŒãŒ âââââââââââââââââ âCOO-ïŒHADHïŒ2H+ ïŒ ããµã³ãã³ ããµã³ãã³ïŒNAD+ïŒH2Oããµã³ãã³ããããã²ããŒã âââââââââââââââââ å°¿é žïŒHADHïŒH+ ïŒ äžæ§èèªãã°ãªã»ããŒã« äžæ§èèªïŒ3H2Oãªããããã€ã³ãªããŒãŒ âââââââââââââââ ã°ãªã»ããŒã«ïŒèèªé ž ã°ã«ã»ããŒã«ïŒNADã°ãªã»ããŒã«ããããã²ããŒãŒ ââââââââââââââââââ ãžããããã·ã¢ã»ãã³ïŒHADH ïŒ 3αâããããã·èæ±é žïŒHAD 3αâããããã·ã¹ããã€ããã ããã²ããŒãŒïŒ3αâHSDïŒ ââââââââââââââââââ ïŒâã±ãèæ±é žïŒHADH æ¬çºæã§çšããããNAD(P)å®éçšçµæç©ã¯
(ã€)ãã«ãªãã·ããŒãŒåã¯ããªãŒã«ãªãã·ããªãã¯
ã¿ãŒãŒ(ã)ãã€ã¢ãã©ãŒãŒåã¯é»åãã€ãªã€ãŒåã³
(ã)ååç©ïŒïŒãããªããããããåè¿°ã®éæ¯ã«
調補ããããã®ã¯äœ¿çšã«äŸ¿å©ã§ãããããã«å¿ èŠ
ã«å¿ããŠç·©è¡å€ããã®çµæç©ã«çµåããããšãã§
ããã 枬å®ãããã¹ãå åã«ãã€ãŠå¿ èŠãªåºè³ªããã
ã¯é µçŽ ãå ããçµæç©ãããã¯å¥ã«çšæããŠçµå
ãããããã¯çš®ã ã®ç©è³ªãããã¯é µçŽ 掻æ§ã®å®é
ã«æ¥µããŠäŸ¿å©ã§æçšã§ããã 以äžã«æ¬çºæã®æ æ§ã瀺ãå®æœäŸã瀺ãã å®æœäŸ ïŒ ç·èæ±é žã®æž¬å® 100ïœïŒ Goodã®ç·©è¡æ¶²ïŒPH6.5ïŒ100mlã«ãã«
ãªãã·ããŒãŒ1000åäœããã€ã¢ãã©ãŒãŒ500åäœã
3αâHSD50åäœãNAD660mgãTriton â100
ã100mgããã³çºè²ååç©ãšããŠååç©No.41ã
10mgååç©No.59ã10mgååç©No.61ã10mgãã
ããã溶解ããè©Šè¬æ¶²ãšããã è©Šéšå®ã«è¡æž 100ÎŒãšäžèšè©Šè¬æ¶²ïŒmlããšãã
37âã«10åéæŸçœ®ããåŸçºè²å€ã®Î»maxã«ããã
åžå 床ãè©Šè¬ç²æ€ãå¯Ÿç §ãšããŠæž¬å®ããäºãäœæ
ããæ€éç·ããè¡æž äžã®ç·èæ±é žæ¿åºŠãç®åºã
ãã è©Šè¬æ¶²ãšããŠãäžèšç·©è¡æ¶²ã«3αâHSDã
NADããã€ã¢ãã©ãŒãŒãããªãã³ïŒžâ100åã³çº
è²ååç©ãšããŠãããããã©ãŸãªãŠã ãã«ãŒ
ïŒNTïŒã15mgçšããä»ã¯ãåèšãšåæ§ã«å®æœã
ãã çµæãã¬ã¹ã¯ãããæ³ïŒGCïŒãçšããŠæž¬å®ã
ãæ°å€ãšå¯Ÿæ¯ããŠç¬¬ïŒè¡šã«äœµèšããã
ãŒã«ãšã¯ãã«ãã³ãŒã³ïŒååç©No.ïŒã10ïŒãã
ãã¯ïŒâããããŒã«â3.5âãžã¹ã«ãã³é žïŒå
åç©No.67.68ïŒãšã60ïŒ ç¡«é žãçšããŠåè¿°ã®
æ¹æ³ã«ãŠçž®åããããåŸãããååç©ãé©éã®
é ¢é žã«æº¶è§£ããé žåéã觊åªãšããŠããžã¡ãã«
ã¢ããåºã®ïŒã€ã®ã¡ãã«åºã®äžæ¹ãè±é¢ããã
ç®çç©ãåŸãã ååç©No.11ã12ã®è£œæ³ ïŒâã¡ãã«âïŒâããããã³ãºã¢ã«ããã€ã
ãšÎ±âïŒïŒ®âã¡ãã«ã¢ããªãïŒâïœâãã«ãšã³ã¹
ã«ãã³é žãšãã¢ã«æ¯ïŒïŒïŒã§åæååç©ç·éé
ã®10åéã®ç¡«é žã«å ãã100âååŸã§120æéæ¹
æãããæ²æŸ±ãç±ããŸãŸã°ã©ã¹ãã€ã«ã¿ãŒã«ãŠ
æ¿ŸéããïŒïŒ ç¡«é žã§æ¿ŸæŽåŸç空也ç¥ãè¡ãç®ç
ç©ãåŸãã ååç©No.19ã20ã®è£œæ³ ïŒâããããã·ãã³ãºã¢ã«ããã€ããšïŒ®ã
âãžã¡ãã«ã¢ããªã³ããã³ïŒ®âããšãã«âãžã
ã³ãžã«ã¢ãã³ãšãã¢ã«æ¯ïŒïŒïŒïŒïŒã§åæåå
ç©ç·ééã®10åéã®80ïŒ ç¡«é žã«å ãã190âå
åŸã§120æéæ¹æãããæ²æ®¿ãç±ããŸãŸã°ã©ã¹
ãã€ã«ã¿ãŒã«ãŠæ¿ŸéããïŒïŒ ç¡«é žã§æ¿ŸæŽåŸç空
也ç¥ãè¡ããç®çç©ãåŸãã ååç©No.21ã22ã®è£œæ³ ïŒãïŒâãžã¯ãããã³ãºã¢ã«ããã€ããšÎ±â
ïŒïŒ®âã¡ãã«ã¢ããªãïŒâïœâãã«ãšã³ã¹ã«ãã³
é žãšãã¢ã«æ¯ïŒïŒïŒã§åæååç©ç·ééã®10å
éã®ç¡«é žã«å ãã100âååŸã§120æéæ¹æã
ããæ²æ®¿ãç±ããŸãŸã°ã©ã¹ãã€ã«ã¿ãŒã«ãŠæ¿Ÿé
ããïŒïŒ ç¡«é žã§æ¿ŸæŽåŸç空也ç¥ãè¡ããç®çç©
ãåŸãã ååç©No.23ã24ã25ã26ã®è£œæ³ ïŒâããããã·ãã³ãºã¢ã«ããã€ããšïŒ®âã
ãšãã«ãžãã³ãžã«ã¢ãã³ïŒNo.23ã24ïŒãããã¯
âããã«ââãã«ã€ãžã³ïŒNo.25ã26ïŒãšã
ã¢ã«æ¯ïŒïŒïŒã§åæååç©ç·ééã®10åéã®ç¡«
é žã«å ãã100âååŸã§120æéæ¹æãããæ²æ®¿
ãç±ããŸãŸã°ã©ã¹ãã€ã«ã¿ãŒã«ãŠæ¿ŸéããïŒïŒ
ç¡«é žã§æ¿ŸæŽåŸç空也ç¥ãè¡ãç®çç©ãåŸãã äžèšä»¥å€ã®ååç©ã«ã€ããŠããäžèšã®æ¹æ³ã«ã
ããŠåæãç®çååç©ã«å¿ããŠéžã¶ããšã«ãã€ãŠ
ãããã¯å ¬ç¥æç®äŸãã°Color Index4å·»ã«èšèŒ
ã®æ¹æ³ã«ãã€ãŠè£œé ã§ããã æ¬çºææ¹æ³ã¯ãåå¿ã«ãã€ãŠNAD(P)ãååŠ
éè«çã«çæããåå¿ç³»ã«ãããåå¿ç©è³ªããã
ã¯é µçŽ 掻æ§ã®æž¬å®ã«é©çšã§ããããããåå¿ç³»ã¯
NADåã³ããããã²ããŒãŒãçšããå€ãã®åå¿
ç³»ãç¥ãããŠããããããç©è³ªãšããŠã°ã«ã³ãŒ
ã¹ãã¬ã©ã¯ããŒã¹ä¹³é žãã¢ã«ã³ãŒã«ããªã³ãŽé žã
ã¢ã«ããããããµã³ãã³ãã³ã¬ã¹ãããŒã«ãèæ±
é žãã©ã¯ããŒãããããã²ããŒãŒïŒLDHïŒæŽ»æ§ã
äžæ§èèªçããããããã ãããã®åå¿ç³»ãå³åŒçã«æ¬¡ã«ç€ºãããåå¿ç³»
ã«ãããåºè³ªãé µçŽ æŽ»æ§ã枬å®ã§ããã ïŒ ã°ã«ã³ãŒã¹ä» ã°ã«ã³ãŒã¹ïŒATPãããœãããŒãŒ âââââââââ ã°ã«ã³ãŒã¹âïŒâãªã³é žïŒADP ã°ã«ã³ãŒã¹âïŒâãªã³é žïŒNAD+ã°ã«ã³ãŒ
ã¹âïŒâ ãªã³é žè±æ°ŽçŽ é µçŽ ââââââââââ âã°ã«ã³ãâÎŽâã©ã¯ãã³ïŒâãªã³é žïŒHA
DHïŒN+ ïŒ ã¬ã©ã¯ããŒã¹ ã¬ã©ã¯ããŒã¹ïŒNAD+ã¬ã©ã¯ããŒã¹ããããã²ããŒ
㌠âââââââââââââââââ âã¬ã©ã¯ããâγâã©ã¯ãã³ïŒNADHïŒ
H+ ïŒ ä¹³é ž ä¹³é žïŒNADã©ã¯ããŒãã»ããããã²ããŒãŒïŒLDHïŒ âââââââââââââââââââââââ ãã«ãã³é žïŒNADHïŒH+ ïŒ ã¢ã«ã³ãŒã« ã¢ã«ã³ãŒã«ïŒãšã¿ããŒã«ïŒïŒNAD+ã¢ã«ã³ãŒã«
ããããã²ããŒãŒ ââââââââââââââââ ã¢ã«ãããïŒã¢ã»ãã¢ã«ãããïŒïŒNADHïŒH+ ïŒ ãªã³ãŽé ž ãªã³ãŽé žïŒNAD+ãã©ãŒãããããã²ããŒãŒ ââââââââââââââââ ãªãã¶ã«é ¢é žïŒNADHïŒH+ ïŒ ã¢ã«ããã âCHOïŒNAD+ïŒH2Oã¢ã«ãããããããã²ããŒãŒ âââââââââââââââââ âCOO-ïŒHADHïŒ2H+ ïŒ ããµã³ãã³ ããµã³ãã³ïŒNAD+ïŒH2Oããµã³ãã³ããããã²ããŒã âââââââââââââââââ å°¿é žïŒHADHïŒH+ ïŒ äžæ§èèªãã°ãªã»ããŒã« äžæ§èèªïŒ3H2Oãªããããã€ã³ãªããŒãŒ âââââââââââââââ ã°ãªã»ããŒã«ïŒèèªé ž ã°ã«ã»ããŒã«ïŒNADã°ãªã»ããŒã«ããããã²ããŒãŒ ââââââââââââââââââ ãžããããã·ã¢ã»ãã³ïŒHADH ïŒ 3αâããããã·èæ±é žïŒHAD 3αâããããã·ã¹ããã€ããã ããã²ããŒãŒïŒ3αâHSDïŒ ââââââââââââââââââ ïŒâã±ãèæ±é žïŒHADH æ¬çºæã§çšããããNAD(P)å®éçšçµæç©ã¯
(ã€)ãã«ãªãã·ããŒãŒåã¯ããªãŒã«ãªãã·ããªãã¯
ã¿ãŒãŒ(ã)ãã€ã¢ãã©ãŒãŒåã¯é»åãã€ãªã€ãŒåã³
(ã)ååç©ïŒïŒãããªããããããåè¿°ã®éæ¯ã«
調補ããããã®ã¯äœ¿çšã«äŸ¿å©ã§ãããããã«å¿ èŠ
ã«å¿ããŠç·©è¡å€ããã®çµæç©ã«çµåããããšãã§
ããã 枬å®ãããã¹ãå åã«ãã€ãŠå¿ èŠãªåºè³ªããã
ã¯é µçŽ ãå ããçµæç©ãããã¯å¥ã«çšæããŠçµå
ãããããã¯çš®ã ã®ç©è³ªãããã¯é µçŽ 掻æ§ã®å®é
ã«æ¥µããŠäŸ¿å©ã§æçšã§ããã 以äžã«æ¬çºæã®æ æ§ã瀺ãå®æœäŸã瀺ãã å®æœäŸ ïŒ ç·èæ±é žã®æž¬å® 100ïœïŒ Goodã®ç·©è¡æ¶²ïŒPH6.5ïŒ100mlã«ãã«
ãªãã·ããŒãŒ1000åäœããã€ã¢ãã©ãŒãŒ500åäœã
3αâHSD50åäœãNAD660mgãTriton â100
ã100mgããã³çºè²ååç©ãšããŠååç©No.41ã
10mgååç©No.59ã10mgååç©No.61ã10mgãã
ããã溶解ããè©Šè¬æ¶²ãšããã è©Šéšå®ã«è¡æž 100ÎŒãšäžèšè©Šè¬æ¶²ïŒmlããšãã
37âã«10åéæŸçœ®ããåŸçºè²å€ã®Î»maxã«ããã
åžå 床ãè©Šè¬ç²æ€ãå¯Ÿç §ãšããŠæž¬å®ããäºãäœæ
ããæ€éç·ããè¡æž äžã®ç·èæ±é žæ¿åºŠãç®åºã
ãã è©Šè¬æ¶²ãšããŠãäžèšç·©è¡æ¶²ã«3αâHSDã
NADããã€ã¢ãã©ãŒãŒãããªãã³ïŒžâ100åã³çº
è²ååç©ãšããŠãããããã©ãŸãªãŠã ãã«ãŒ
ïŒNTïŒã15mgçšããä»ã¯ãåèšãšåæ§ã«å®æœã
ãã çµæãã¬ã¹ã¯ãããæ³ïŒGCïŒãçšããŠæž¬å®ã
ãæ°å€ãšå¯Ÿæ¯ããŠç¬¬ïŒè¡šã«äœµèšããã
ãè¡šã
å®æœäŸ ïŒ
LDH掻æ§ã®æž¬å®
100ïœïŒ Goodã®ç·©è¡æ¶²ïŒPH8.2ïŒ100mlã«ãã«
ãªãã·ããŒãŒ1000åäœãMPMS1.5mgãâä¹³é ž
ãããªãŠã 50mgãNAD100mgãTriton â100
100mgããã³çºè²ååç©ãšããŠååç©No.53ã10
mgååç©No.69ã10mgååç©No.75ã10mgããã
ãã溶解ããè©Šè¬æ¶²ãšããã è©Šè¬æ¶²ãäºã37âã§10åéå æž©ããåŸãè¡æž
20ÎŒãæ·»å ããæ£ç¢ºã«ïŒååŸãšïŒååŸã®åžå 床
ãçºè²å€ã®Î»maxã«ãããŠè©Šè¬ç²æ€ãå¯Ÿç §ãšããŠ
枬å®ãããïŒååŸãšïŒååŸã®åžå 床差ãèšç®ãã
äºãäœæããæ€éç·ãããè¡æž äžã®LDH掻æ§ã
ç®åºããã è©Šè¬æ¶²ãšããŠäžèšç·©è¡æ¶²ã«MPMSãâä¹³é ž
ãããªãŠã ãããªãã³ïŒžâ100ãNADã10mgã®ã
ããããã©ãŸãªãŠã ãã«ãŒïŒNTïŒã溶解ãã液
ãçšããä»ã¯äžèšãšåæ§ã«å®æœããã çµæãUVæ³ãçšããŠæž¬å®ããæ°å€ãšå¯Ÿæ¯ããŠ
第ïŒè¡šã«äœµèšããã
ãªãã·ããŒãŒ1000åäœãMPMS1.5mgãâä¹³é ž
ãããªãŠã 50mgãNAD100mgãTriton â100
100mgããã³çºè²ååç©ãšããŠååç©No.53ã10
mgååç©No.69ã10mgååç©No.75ã10mgããã
ãã溶解ããè©Šè¬æ¶²ãšããã è©Šè¬æ¶²ãäºã37âã§10åéå æž©ããåŸãè¡æž
20ÎŒãæ·»å ããæ£ç¢ºã«ïŒååŸãšïŒååŸã®åžå 床
ãçºè²å€ã®Î»maxã«ãããŠè©Šè¬ç²æ€ãå¯Ÿç §ãšããŠ
枬å®ãããïŒååŸãšïŒååŸã®åžå 床差ãèšç®ãã
äºãäœæããæ€éç·ãããè¡æž äžã®LDH掻æ§ã
ç®åºããã è©Šè¬æ¶²ãšããŠäžèšç·©è¡æ¶²ã«MPMSãâä¹³é ž
ãããªãŠã ãããªãã³ïŒžâ100ãNADã10mgã®ã
ããããã©ãŸãªãŠã ãã«ãŒïŒNTïŒã溶解ãã液
ãçšããä»ã¯äžèšãšåæ§ã«å®æœããã çµæãUVæ³ãçšããŠæž¬å®ããæ°å€ãšå¯Ÿæ¯ããŠ
第ïŒè¡šã«äœµèšããã
ãè¡šã
å®æœäŸ ïŒ
äžæ§èèªã®æž¬å®
100ïœïŒ Goodã®ç·©è¡æ¶²ïŒPH6.75ïŒ100mlã«ã
ã«ãªãã·ããŒãŒ1000åäœããã€ã¢ãã©ãŒãŒ500å
äœããªããããã€ã³ãªããŒãŒ50000åäœãã°ãªã»
ããŒã«ããããã²ããŒãŒ5000åäœNAD500mgã
Triton â100ã100mgãããã³çºè²ååç©ãšã
ãŠååç©No.71ã10mgååç©No.63ã10mgåå
ç©No.65ã10mgã溶解ããŠè©Šè¬æ¶²ãšããã è©Šéšç®¡ã«è¡æž 20ÎŒãšäžèšè©Šè¬æ¶²ïŒmlããšãã
37âã«10åéæŸçœ®ããåŸãçºè²å€ã®Î»maxã«ãã
ãåžå 床ãè©Šè¬ç²æ€ãå¯Ÿç §ãšããŠæž¬å®ããäºãäœ
æããæ€éç·ããè¡æž äžã®äžæ§èèªæ¿åºŠãç®åºã
ãã è©Šè¬æ¶²ãšããŠäžèšç·©è¡æ¶²ã«ãã€ã¢ãã©ãŒãŒããª
ããããã€ã³ãªããŒãŒãã°ãªã»ããŒã«ããããã²
ããŒãŒãNADãããªãã³ïŒžâ100åã³15mgã®ãã
ãããã©ãŸãªãŠã ãã«ãŒãçšããä»ã¯äžèšãšåæ§
ã«å®æœããã çµæãã°ãªã»ããŒã«ãªãã·ããŒãŒããã«ãªãã·
ããŒãŒã䜿çšããç³»(A)ã§æž¬å®ããæ°å€ã察æ¯ããŠ
第ïŒè¡šã«äœµèšããã
ã«ãªãã·ããŒãŒ1000åäœããã€ã¢ãã©ãŒãŒ500å
äœããªããããã€ã³ãªããŒãŒ50000åäœãã°ãªã»
ããŒã«ããããã²ããŒãŒ5000åäœNAD500mgã
Triton â100ã100mgãããã³çºè²ååç©ãšã
ãŠååç©No.71ã10mgååç©No.63ã10mgåå
ç©No.65ã10mgã溶解ããŠè©Šè¬æ¶²ãšããã è©Šéšç®¡ã«è¡æž 20ÎŒãšäžèšè©Šè¬æ¶²ïŒmlããšãã
37âã«10åéæŸçœ®ããåŸãçºè²å€ã®Î»maxã«ãã
ãåžå 床ãè©Šè¬ç²æ€ãå¯Ÿç §ãšããŠæž¬å®ããäºãäœ
æããæ€éç·ããè¡æž äžã®äžæ§èèªæ¿åºŠãç®åºã
ãã è©Šè¬æ¶²ãšããŠäžèšç·©è¡æ¶²ã«ãã€ã¢ãã©ãŒãŒããª
ããããã€ã³ãªããŒãŒãã°ãªã»ããŒã«ããããã²
ããŒãŒãNADãããªãã³ïŒžâ100åã³15mgã®ãã
ãããã©ãŸãªãŠã ãã«ãŒãçšããä»ã¯äžèšãšåæ§
ã«å®æœããã çµæãã°ãªã»ããŒã«ãªãã·ããŒãŒããã«ãªãã·
ããŒãŒã䜿çšããç³»(A)ã§æž¬å®ããæ°å€ã察æ¯ããŠ
第ïŒè¡šã«äœµèšããã
Claims (1)
- ãç¹èš±è«æ±ã®ç¯å²ã ïŒ NAD(P)ãå«æããè©Šæããã«ãªãã·ã
ãŒãŒããã€ã¢ãã©ãŒãŒåã¯é»åãã€ãªã¢ãŒåã³
äžèšäžè¬åŒïŒïŒã§è¡šãããååç©ãšå ±ã«æ°Žæ§
液äžã«ä¿æããçè²ãã溶液ã®åžå 床ã枬å®ãã
ããšãç¹åŸŽãšããNAD(P)ã®å®éæ¹æ³ã äžè¬åŒïŒïŒ åŒäžïŒ¹ã¯æ°ŽçŽ ãããã¯ããããã·ã«ã瀺ãã
R1ãR2ãR3ã¯åäžãããã¯ç°ãªã€ãŠããäžèšäž
è¬åŒïŒïŒãïŒïŒãŸãã¯ïŒïŒã§è¡šãããåºã瀺
ãã ã¯æ°ŽçŽ ãããããã·ã«ãã¢ããã眮æã¢ã
ããã¢ã«ãã«ã眮æã¢ã«ãã«ãã¢ã«ã±ãã«ãã¢ãª
ãŒã«ã眮æã¢ãªãŒã«ãã¢ã·ã«ãããã²ã³ããã
ããã¹ã«ããã«ã«ããã·ãã¢ã«ã³ãã·ã瀺ããïœ
ã¯ïŒãïŒåã¯ïŒã瀺ããR1ãR2ãR3ã®å°ãªããš
ãïŒã€ã®ïŒºã¯ããããã·ã«ãã¢ããåã¯çœ®æã¢ã
ãã§ãããåãZnã«ãããåã¯åäžãããã¯
ç°ãªã€ãŠããŠãããã ïŒ (ã€) ãã«ãªãã·ããŒãŒ (ã) ãã€ã¢ãã©ãŒãŒåã¯é»åãã€ãªã¢ãŒåã³ (ã) äžè¬åŒïŒïŒã§è¡šãããååç© ãããªãNAD(P)å®éçšçµæç©ã
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59037623A JPS60180600A (ja) | 1984-02-29 | 1984-02-29 | éå åãã³ãã³ã¢ããã»ã¢ããã³ã»ãžãã¯ã¬ãªããã®å®éæ¹æ³ |
| US06/706,404 US4824779A (en) | 1984-02-29 | 1985-02-28 | Method for the determination of the reduced form of nicotinamide adenine dinucleotide |
| EP85301391A EP0153872B1 (en) | 1984-02-29 | 1985-02-28 | Method for the determination of the reduced form of nicotinamide adenine dinucleotide |
| DE8585301391T DE3585238D1 (de) | 1984-02-29 | 1985-02-28 | Verfahren zur bestimmung des reduzierten zustands von nicotinamid-adenin-dinukleotid. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59037623A JPS60180600A (ja) | 1984-02-29 | 1984-02-29 | éå åãã³ãã³ã¢ããã»ã¢ããã³ã»ãžãã¯ã¬ãªããã®å®éæ¹æ³ |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60180600A JPS60180600A (ja) | 1985-09-14 |
| JPH0542271B2 true JPH0542271B2 (ja) | 1993-06-28 |
Family
ID=12502758
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59037623A Granted JPS60180600A (ja) | 1984-02-29 | 1984-02-29 | éå åãã³ãã³ã¢ããã»ã¢ããã³ã»ãžãã¯ã¬ãªããã®å®éæ¹æ³ |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US4824779A (ja) |
| EP (1) | EP0153872B1 (ja) |
| JP (1) | JPS60180600A (ja) |
| DE (1) | DE3585238D1 (ja) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60217900A (ja) * | 1984-04-13 | 1985-10-31 | Kyowa Medetsukusu Kk | ã¡ã«ã«ããåºå«æååç©ã®å®éæ¹æ³ |
| JPS62296A (ja) * | 1985-06-26 | 1987-01-06 | Kyowa Medetsukusu Kk | éé žåæ°ŽçŽ ã®å®éæ¹æ³ |
| US4898813A (en) * | 1986-04-04 | 1990-02-06 | Albarella James P | Catalytic test composition intended to produce a range of colors |
| US5190863A (en) * | 1990-06-29 | 1993-03-02 | Miles Inc. | Composition for determining the presence or concentration of D-β-hydroxybutyrate |
| US6166013A (en) * | 1998-07-30 | 2000-12-26 | Abbott Laboratories | Glucocortiocoid-selective agents |
| US6656697B1 (en) * | 1998-09-28 | 2003-12-02 | Lifescan, Inc. | Diagnostics based on tetrazolium compounds |
| CA2404421A1 (en) * | 2000-03-28 | 2001-10-04 | Tianmei Ouyang | Reagent systems for detecting the presence of a reduced cofactor in a sample and methods for using the same |
| US20040096927A1 (en) * | 2000-09-13 | 2004-05-20 | Chittock Roger Steward | Method for the detection and measurement of biomass |
| KR20140142262A (ko) * | 2012-03-29 | 2014-12-11 | 믞ì°ë¹ì ê°ê°êŸž ê°ë¶ìí€ê°ìŽì€ | ížëŠ¬ì늎ë©íê³ íí©ë¬Œ, ì°©ì ìì§ ì¡°ì±ë¬Œ, ì»¬ë¬ íí°, ì¡ì íì ì¥ì¹ ë° ì êž° el íì ì¥ì¹ |
| JP6064598B2 (ja) * | 2012-12-28 | 2017-01-25 | Jsræ ªåŒäŒç€Ÿ | çè²çµæç©ãã«ã©ãŒãã£ã«ã¿åã³è¡šç€ºçŽ å |
| JP6350245B2 (ja) * | 2014-02-10 | 2018-07-04 | äžè±ã±ãã«ã«æ ªåŒäŒç€Ÿ | çè²æš¹èçµæç©ãã«ã©ãŒãã£ã«ã¿ã液æ¶è¡šç€ºè£ 眮åã³ææ©ïœ ïœè¡šç€ºè£ 眮 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4036863A (en) * | 1974-04-01 | 1977-07-19 | Polaroid Corporation | Trinapthylmethane compounds |
| CA1141637A (en) * | 1979-10-10 | 1983-02-22 | Erma C. Cameron | Cofactor indicator compositions |
| DE3261213D1 (en) * | 1981-01-22 | 1984-12-20 | Ciba Geigy Ag | Process for the production of leuco triaryl methane compounds |
| US4448446A (en) * | 1981-12-09 | 1984-05-15 | The Sherwin-Williams Company | Process for the purification of triphenylmethane compounds and pressure sensitive copysheet containing same |
| LU83955A1 (fr) * | 1982-02-18 | 1983-09-02 | Camille Jean Heusghem | Composition et procede pour dosages avec cycle enzymatique amplificateur a nad+ |
| DE3236388A1 (de) * | 1982-10-01 | 1984-04-05 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur selektiven herstellung reduzierter sauerstoffspezies und hierfuer geeignete reagentien |
| JPS59205999A (ja) * | 1983-05-10 | 1984-11-21 | Wako Pure Chem Ind Ltd | éå åè£é µçŽ ã®å®éæ¹æ³ |
| JPH0764986B2 (ja) * | 1984-03-02 | 1995-07-12 | åå çŽè¬å·¥æ¥æ ªåŒäŒç€Ÿ | æ°èŠãªçºè²è©Šè¬ |
-
1984
- 1984-02-29 JP JP59037623A patent/JPS60180600A/ja active Granted
-
1985
- 1985-02-28 US US06/706,404 patent/US4824779A/en not_active Expired - Lifetime
- 1985-02-28 DE DE8585301391T patent/DE3585238D1/de not_active Expired - Lifetime
- 1985-02-28 EP EP85301391A patent/EP0153872B1/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| EP0153872B1 (en) | 1992-01-22 |
| EP0153872A3 (en) | 1987-11-25 |
| US4824779A (en) | 1989-04-25 |
| JPS60180600A (ja) | 1985-09-14 |
| DE3585238D1 (de) | 1992-03-05 |
| EP0153872A2 (en) | 1985-09-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JPH0542272B2 (ja) | ||
| JPS6033479B2 (ja) | éé žåæ°ŽçŽ ã®å®éæ¹æ³ | |
| JP2003513025A (ja) | ïŒâïŒã¢ãªããïŒâïŒâããã¿ã¬ã³ã¹ã«ãããŒãé¡äŒŒç©ããã³ããããåæç©æ€åºæ€å®ã§çšããäœ¿çš | |
| JPH0542271B2 (ja) | ||
| JPH10130247A (ja) | ã¬ããã¯ã¹æŽ»æ§ååç©ããã³ãã®äœ¿çš | |
| JPH0764986B2 (ja) | æ°èŠãªçºè²è©Šè¬ | |
| EP0488756B1 (en) | Oxidizable color producing reagent | |
| JPS62296A (ja) | éé žåæ°ŽçŽ ã®å®éæ¹æ³ | |
| EP3131882B1 (en) | Phenazinium mediators | |
| JP2516381B2 (ja) | éé žåæ°ŽçŽ ã®å®éæ¹æ³åã³ãã®å®éçšè©Šè¬ | |
| JP3036806B2 (ja) | äœæ¶²äžã®åŸ®éæåå®éæ³ | |
| JP2590124B2 (ja) | 氎溶æ§ããã©ãŸãªãŠã ååç©ããã³ãã®ååç©ãçšããéå æ§ç©è³ªã®æž¬å®æ¹æ³ | |
| WO1985003942A1 (fr) | Derives de triphenylmethane et procede de determination de substances oxydantes les utilisant en tant que composant de formation de couleur | |
| JPS6229578A (ja) | âã¢ã·ã«âãžãããã¬ãŸã«ãã£ã³èªå°äœåã³ãã®è£œæ³ | |
| US4711963A (en) | 4-amino-2-methyl-3-phenyl-1-(2,4,6-trichlorophenyl)-3-pyrazolin-5-one | |
| JP5231062B2 (ja) | ã¹ã«ããã§ãã«ãžã¡ãã«ãã©ãŸãã³ã®è£œé æ¹æ³ | |
| JP3980683B2 (ja) | éå åãã³ãã³ã¢ããè£é µçŽ ã®æž¬å®çšè©Šè¬ããã³æž¬å®æ¹æ³ | |
| US5792619A (en) | Assay using oxidative chromogenic reagent | |
| JPH0423999A (ja) | ã«ã«ã·ãŠã ã®å®éæ³ | |
| JP2566790B2 (ja) | ãžãã§ãã«ã¢ãã³èªå°äœ | |
| JP2001352999A (ja) | é žåçºè²è©Šè¬ | |
| JPH01128799A (ja) | éå åè£é µçŽ ã®å®éæ³ | |
| JPH0699403B2 (ja) | ã¢ããã¢ã³ãããªã³èªå°äœåã³ãã®äœ¿çšæ³ | |
| JPS6219100A (ja) | èæ±é žã®å®éæ³ | |
| JP2547375B2 (ja) | ïŒïŒïŒâ²ïŒïŒïŒïŒâ²âããã©ã¢ã«ãã«ãã³ããžã³ã®ã¹ã«ãã¢ã«ãã«èªå°äœ |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |