JPH0555519B2 - - Google Patents

Info

Publication number: JPH0555519B2
Authority: JP; Japan
Prior art keywords: adf; cells; atl; medium; ala
Prior art date: 1985-07-19
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

JP60159749A

Other languages

English (en)

Japanese (ja)

Other versions

JPS6219532A (ja

Inventor

Junji Yodoi

Keisuke Teshigawara

Atsushi Tagaya

Michuki Maeda

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Ajinomoto Co Inc

Original Assignee

Ajinomoto Co Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-07-19

Filing date

1985-07-19

Publication date

1993-08-17

1985-07-19 Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc

1985-07-19 Priority to JP60159749A priority Critical patent/JPS6219532A/ja

1987-01-28 Publication of JPS6219532A publication Critical patent/JPS6219532A/ja

1993-08-17 Publication of JPH0555519B2 publication Critical patent/JPH0555519B2/ja

Status Granted legal-status Critical Current

Links

238000000034 method Methods 0.000 claims description 14
102000010789 Interleukin-2 Receptors Human genes 0.000 claims description 3
108010038453 Interleukin-2 Receptors Proteins 0.000 claims description 3
238000002523 gelfiltration Methods 0.000 claims description 2
239000000411 inducer Substances 0.000 claims description 2
125000003275 alpha amino acid group Chemical group 0.000 claims 1
210000004027 cell Anatomy 0.000 description 75
208000009746 Adult T-Cell Leukemia-Lymphoma Diseases 0.000 description 29
208000016683 Adult T-cell leukemia/lymphoma Diseases 0.000 description 29
201000006966 adult T-cell leukemia Diseases 0.000 description 29
102000004169 proteins and genes Human genes 0.000 description 20
108090000623 proteins and genes Proteins 0.000 description 20
239000002609 medium Substances 0.000 description 18
102000000588 Interleukin-2 Human genes 0.000 description 17
108010002350 Interleukin-2 Proteins 0.000 description 17
108091003079 Bovine Serum Albumin Proteins 0.000 description 16
239000000427 antigen Substances 0.000 description 16
102000036639 antigens Human genes 0.000 description 16
108091007433 antigens Proteins 0.000 description 16
239000012894 fetal calf serum Substances 0.000 description 15
230000000694 effects Effects 0.000 description 14
239000012980 RPMI-1640 medium Substances 0.000 description 10
238000012258 culturing Methods 0.000 description 10
210000005259 peripheral blood Anatomy 0.000 description 10
239000011886 peripheral blood Substances 0.000 description 10
239000000126 substance Substances 0.000 description 10
239000012228 culture supernatant Substances 0.000 description 9
229910052588 hydroxylapatite Inorganic materials 0.000 description 8
XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 8
UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 8
239000012506 Sephacryl® Substances 0.000 description 7
150000001413 amino acids Chemical group 0.000 description 7
210000001744 T-lymphocyte Anatomy 0.000 description 6
238000004128 high performance liquid chromatography Methods 0.000 description 6
238000000746 purification Methods 0.000 description 6
241000532370 Atla Species 0.000 description 5
238000004587 chromatography analysis Methods 0.000 description 5
238000004440 column chromatography Methods 0.000 description 5
MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 5
238000001641 gel filtration chromatography Methods 0.000 description 5
229930182555 Penicillin Natural products 0.000 description 4
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical group N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 4
208000032839 leukemia Diseases 0.000 description 4
239000000203 mixture Substances 0.000 description 4
229940049954 penicillin Drugs 0.000 description 4
210000002966 serum Anatomy 0.000 description 4
239000000243 solution Substances 0.000 description 4
229960005322 streptomycin Drugs 0.000 description 4
WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
102000000589 Interleukin-1 Human genes 0.000 description 3
108010002352 Interleukin-1 Proteins 0.000 description 3
206010028980 Neoplasm Diseases 0.000 description 3
239000003814 drug Substances 0.000 description 3
238000010828 elution Methods 0.000 description 3
239000012847 fine chemical Substances 0.000 description 3
239000001963 growth medium Substances 0.000 description 3
230000001939 inductive effect Effects 0.000 description 3
238000004519 manufacturing process Methods 0.000 description 3
238000004007 reversed phase HPLC Methods 0.000 description 3
101800000263 Acidic protein Proteins 0.000 description 2
CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
239000006144 Dulbecco’s modiﬁed Eagle's medium Substances 0.000 description 2
229920001917 Ficoll Polymers 0.000 description 2
206010061598 Immunodeficiency Diseases 0.000 description 2
208000029462 Immunodeficiency disease Diseases 0.000 description 2
ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 2
102000008072 Lymphokines Human genes 0.000 description 2
108010074338 Lymphokines Proteins 0.000 description 2
PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
241000700605 Viruses Species 0.000 description 2
210000003719 b-lymphocyte Anatomy 0.000 description 2
238000004113 cell culture Methods 0.000 description 2
238000011098 chromatofocusing Methods 0.000 description 2
238000000684 flow cytometry Methods 0.000 description 2
239000012510 hollow fiber Substances 0.000 description 2
230000007813 immunodeficiency Effects 0.000 description 2
238000001727 in vivo Methods 0.000 description 2
230000006698 induction Effects 0.000 description 2
210000000265 leukocyte Anatomy 0.000 description 2
239000003226 mitogen Substances 0.000 description 2
239000013642 negative control Substances 0.000 description 2
102000005962 receptors Human genes 0.000 description 2
108020003175 receptors Proteins 0.000 description 2
238000011160 research Methods 0.000 description 2
210000004988 splenocyte Anatomy 0.000 description 2
229940124597 therapeutic agent Drugs 0.000 description 2
238000000108 ultra-filtration Methods 0.000 description 2
DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
102000019260 B-Cell Antigen Receptors Human genes 0.000 description 1
108010012919 B-Cell Antigen Receptors Proteins 0.000 description 1
210000001239 CD8-positive, alpha-beta cytotoxic T lymphocyte Anatomy 0.000 description 1
241000283707 Capra Species 0.000 description 1
108010038061 Chymotrypsinogen Proteins 0.000 description 1
102000018832 Cytochromes Human genes 0.000 description 1
108010052832 Cytochromes Proteins 0.000 description 1
229930182566 Gentamicin Natural products 0.000 description 1
CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
108060003951 Immunoglobulin Proteins 0.000 description 1
241001494479 Pecora Species 0.000 description 1
239000012979 RPMI medium Substances 0.000 description 1
239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 1
BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
102000016266 T-Cell Antigen Receptors Human genes 0.000 description 1
108010092262 T-Cell Antigen Receptors Proteins 0.000 description 1
238000010521 absorption reaction Methods 0.000 description 1
239000013543 active substance Substances 0.000 description 1
239000000654 additive Substances 0.000 description 1
230000000996 additive effect Effects 0.000 description 1
238000005273 aeration Methods 0.000 description 1
238000001042 affinity chromatography Methods 0.000 description 1
230000001363 autoimmune Effects 0.000 description 1
102000015736 beta 2-Microglobulin Human genes 0.000 description 1
108010081355 beta 2-Microglobulin Proteins 0.000 description 1
230000004071 biological effect Effects 0.000 description 1
OWMVSZAMULFTJU-UHFFFAOYSA-N bis-tris Chemical compound OCCN(CCO)C(CO)(CO)CO OWMVSZAMULFTJU-UHFFFAOYSA-N 0.000 description 1
229940098773 bovine serum albumin Drugs 0.000 description 1
201000011510 cancer Diseases 0.000 description 1
229910002092 carbon dioxide Inorganic materials 0.000 description 1
239000001569 carbon dioxide Substances 0.000 description 1
230000024245 cell differentiation Effects 0.000 description 1
210000003855 cell nucleus Anatomy 0.000 description 1
230000004663 cell proliferation Effects 0.000 description 1
238000006243 chemical reaction Methods 0.000 description 1
239000000470 constituent Substances 0.000 description 1
238000000502 dialysis Methods 0.000 description 1
201000010099 disease Diseases 0.000 description 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
229940079593 drug Drugs 0.000 description 1
238000001493 electron microscopy Methods 0.000 description 1
230000007613 environmental effect Effects 0.000 description 1
210000003743 erythrocyte Anatomy 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
230000001605 fetal effect Effects 0.000 description 1
-1 fluorecein isothiocyanate Chemical class 0.000 description 1
238000005194 fractionation Methods 0.000 description 1
230000005251 gamma ray Effects 0.000 description 1
238000001502 gel electrophoresis Methods 0.000 description 1
238000005227 gel permeation chromatography Methods 0.000 description 1
229960002518 gentamicin Drugs 0.000 description 1
239000011521 glass Substances 0.000 description 1
ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
210000002443 helper t lymphocyte Anatomy 0.000 description 1
102000018358 immunoglobulin Human genes 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
208000015181 infectious disease Diseases 0.000 description 1
238000004255 ion exchange chromatography Methods 0.000 description 1
238000004811 liquid chromatography Methods 0.000 description 1
239000012528 membrane Substances 0.000 description 1
239000002245 particle Substances 0.000 description 1
239000004033 plastic Substances 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
102000004196 processed proteins & peptides Human genes 0.000 description 1
108090000765 processed proteins & peptides Proteins 0.000 description 1
230000035755 proliferation Effects 0.000 description 1
230000001105 regulatory effect Effects 0.000 description 1
238000004366 reverse phase liquid chromatography Methods 0.000 description 1
238000005185 salting out Methods 0.000 description 1
238000000926 separation method Methods 0.000 description 1
229910000077 silane Inorganic materials 0.000 description 1
229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
235000017557 sodium bicarbonate Nutrition 0.000 description 1
210000001541 thymus gland Anatomy 0.000 description 1
210000001519 tissue Anatomy 0.000 description 1
230000009466 transformation Effects 0.000 description 1
241001430294 unidentified retrovirus Species 0.000 description 1
238000005406 washing Methods 0.000 description 1

Landscapes

Preparation Of Compounds By Using Micro-Organisms (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Peptides Or Proteins (AREA)

JP60159749A 1985-07-19 1985-07-19 インタ−ロイキン２レセプタ−発現誘導物質ａ Granted JPS6219532A (ja)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP60159749A JPS6219532A (ja)	1985-07-19	1985-07-19	インタ−ロイキン２レセプタ−発現誘導物質ａ

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP60159749A JPS6219532A (ja)	1985-07-19	1985-07-19	インタ−ロイキン２レセプタ−発現誘導物質ａ

Publications (2)

Publication Number	Publication Date
JPS6219532A JPS6219532A (ja)	1987-01-28
JPH0555519B2 true JPH0555519B2 (fr)	1993-08-17

Family

ID=15700422

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP60159749A Granted JPS6219532A (ja)	1985-07-19	1985-07-19	インタ−ロイキン２レセプタ−発現誘導物質ａ

Country Status (1)

Country	Link
JP (1)	JPS6219532A (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPH0794331A (ja) *	1993-09-27	1995-04-07	Matsushita Electric Ind Co Ltd	ラインフィルタ

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP2633295B2 (ja) *	1987-06-12	1997-07-23	味の素株式会社	ヒトａｄｆをコードする遺伝子
JPWO2007138961A1 (ja)	2006-05-29	2009-10-08	レドックス・バイオサイエンス株式会社	マクロファージ遊走阻止因子阻害剤
EP2363475A4 (fr)	2008-11-26	2012-07-18	Redox Bioscience Inc	Méthode de criblage, composition contenant une substance sélectionnée par la méthode de criblage, et substance de liaison

1985
- 1985-07-19 JP JP60159749A patent/JPS6219532A/ja active Granted

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPH0794331A (ja) *	1993-09-27	1995-04-07	Matsushita Electric Ind Co Ltd	ラインフィルタ

Also Published As

Publication number	Publication date
JPS6219532A (ja)	1987-01-28

Legal Events

Date	Code	Title	Description
2004-08-10	R250	Receipt of annual fees	Free format text: JAPANESE INTERMEDIATE CODE: R250
2005-07-19	EXPY	Cancellation because of completion of term

Publication	Publication Date	Title
US4816561A (en)	1989-03-28	Biologically active polypeptides
Desreumaux et al.	1992	Interleukin 5 messenger RNA expression by eosinophils in the intestinal mucosa of patients with coeliac disease.
US5240912A (en)	1993-08-31	Transforming growth factor (TGF) peptides
US4002602A (en)	1977-01-11	Ubiquitous immunopoietic polypeptide (UBIP) and methods
US4863899A (en)	1989-09-05	Biologically active polypeptides
Wahl et al.	1985	Spontaneous production of fibroblast-activating factor (s) by synovial inflammatory cells. A potential mechanism for enhanced tissue destruction.
JPS5813397A (ja)	1983-01-25	免疫インタ−フエロン及びそのｍＲＮＡの製造方法
JPS61501514A (ja)	1986-07-24	免疫抑制因子
US4167557A (en)	1979-09-11	Ubiquitous immunopoietic polypeptide (UBIP) and methods
JPH0555519B2 (fr)	1993-08-17
JPS6216430A (ja)	1987-01-24	インタ−ロイキン２レセプタ−発現誘導物質ａ
FI91484C (fi)	1994-07-11	Menetelmä uuden solun kasvua säätelevän tekijän, onkostatiini M:n valmistamiseksi
EP0132021B1 (fr)	1991-10-30	Polypeptides TGF, oligopeptidiques antigéniques dérivés de ceux-ci, anticorps produits de ceux-ci
Becker et al.	1977	Crystallographic studies of bovine beta2-microglobulin.
EP0132359A2 (fr)	1985-01-30	Interleucine-2 naturelle humaine et sa préparation
JPH02288899A (ja)	1990-11-28	成熟肝実質細胞増殖因子（ｉ）
CA2072626A1 (fr)	1992-03-15	Medicament liberant du cd14
Dainiak et al.	1988	Acetylated lipoproteins impair erythroid growth factor release from endothelial cells.
JPS6216420A (ja)	1987-01-24	インタ−ロイキン２レセプタ−発現誘導物質ｂ
JPS6219533A (ja)	1987-01-28	インタ−ロイキン２レセプタ−発現誘導物質ｂ
EP0117486A2 (fr)	1984-09-05	Méthode de croissance et de purification de lignées de cellules humaines à partir de populations de cellules hétérogènes de tissu en culture
WO1992017500A1 (fr)	1992-10-15	Nouveau facteur d'amplification de megacaryocytes et son procede de production
CA1341536C (fr)	2007-07-24	Facteurs de croissance transformants peptidiques
JPH0657157B2 (ja)	1994-08-03	Ｂ細胞分化因子の製造法
JPH0657720B2 (ja)	1994-08-03	Ｂ細胞分化因子

JPH0555519B2 - - Google Patents

Info

Links

Landscapes

Priority Applications (1)

Applications Claiming Priority (1)

Publications (2)

Family

ID=15700422

Family Applications (1)

Country Status (1)

Cited By (1)

Families Citing this family (3)

Cited By (1)

Also Published As

Similar Documents

Legal Events