JPH0566152B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a denatured blood component removal filter for passing leukocytes in blood or a blood cell suspension and capturing only denatured blood components. More specifically, the fibers have a diameter of 3 to 10 μm, a bulk density of 0.05 to 0.45 g/ ^cm2 , are made of a nonwoven fabric fixed by mutual entanglement of fibers, and have a thickness of 0.05 to 0.45 g/cm2.
0.35mm and its total surface area is 15~2000
cm ² /500ml - This relates to a denatured blood component removal filter for passing leukocytes and capturing only denatured blood components, which is stored blood.

Recently, procedures have been performed to remove denatured blood components from blood prior to blood transfusion. This denatured blood component often occurs when blood is stored,
In particular, as they are stored for a long period of time, their number increases and grows significantly.

For example, as a filter for removing large denatured blood components,
There is a 20 μm woven fabric filter No. 111960, but it is a filter that only removes large denatured blood components, and it removes microaggregates, which are small denatured blood components in blood, that is, 15 μm in size.
Those with a diameter of ~20 μm could not be completely removed. There is a problem in that even these small aggregates tend to cause pulmonary capillary occlusion during large-scale blood transfusion. For example, denatured blood component removal filters that can completely remove these small aggregates include JP-A No. 51-4891.
There is a denatured blood component removal filter packed with fibers with a thickness of 0.381 mm or more, but even if micro aggregates can be completely removed, the processing speed is slow at around 20 ml/min, and the processing speed is slow at around 500 ml of blood. When used in blood centers, hospitals, etc., the processing time is a little too long, and if the blood or blood cell suspension is left at room temperature during this time, there is a problem that denaturation of the blood progresses.

The inventor has discovered that large degenerated blood components in the blood
It can completely remove aggregated substances up to 15-20μm,
Moreover, as a result of intensive research aimed at developing a filter for removing denatured blood components that can be processed at high speed, we found that it consists of fibers with a diameter of 3 to 10 μm and a bulk density of 0.05 to 10 μm.
0.45g/ ^cm2 , made of non-woven fabric fixed by intertwining of fibers, its thickness is 0.05-0.35mm, and its total surface area is ^15-2000cm2 /500ml
-A filter for removing denatured blood components, which is stored blood,
It can remove all denatured blood components from large denatured blood components to aggregates of 15 to 20 μm.
It can also capture sticky substances such as denatured proteins that exist in long-term stored blood, and it
It has been found that high-speed processing on the order of ml/minute is possible.

Blood contains red blood cells, white blood cells, platelets, plasma, etc., and the diameter of red blood cells is 8 to 10 μm, and the diameter of white blood cells is 12 to 20 μm. Agglutinated substances of denatured blood components are as small as 15 to 20 μm.
The difference in size from white blood cells disappears, but if the thickness of the fibers used in the denatured blood component removal filter is made extremely thin and the gaps between the fibers are made small, white blood cells and red blood cells can pass through. and,
Agglutinated substances in blood are captured; in this case, the fibers used are fibers that have the property of adsorbing denatured blood components, and also capture sticky substances such as denatured proteins that exist in long-term stored blood. It has been found that fibers that have the property of

Preferred fibers in the present invention, that is, fibers that have the property of adsorbing denatured blood components and capturing sticky substances such as denatured proteins, include:
Examples of synthetic fibers include polyester, polyamide, aromatic polyamide, and polyacrylonitrile fibers, and examples of recycled fibers include cypra ammonium rayon.

In the present invention, the diameter of the fibers ranges from 3 to 10 μm, preferably from 4 to 8 μm. Creating fibers with a diameter of 3 to 10 μm,
It is generally difficult to make this into a uniform nonwoven fabric. Examples of methods for producing fibers of 10 μm or less include the melt-blowing method (Melt-blowing method).
The fibers and nonwoven fabrics of the present invention were produced by this method. However, the fibers used in the present invention are not limited to this method. When the fiber diameter becomes smaller than 3 μm, leukocytes are also captured, and
When the diameter exceeds 10 μm, the gaps between the fibers become large, and the aggregated substances cannot be completely removed by high-speed processing.

The nonwoven fabric of the present invention is a fibrous material in which the fibers are fixed in position by mutual entanglement of the fibers, and the method of fixing the fibers is by applying heat near the melting point of these fibers. Fix it or
There are fixing methods using adhesives, and these can of course be used in the present invention, but when the fibers become thin like the fibers used in the present invention, the fibers are made by melt blowing, so air blowing, high pressure, etc. By simply entangling the fibers with each other using a method such as steam blowing, the fibers are fixed in place, and the nonwoven fabric created in this way can be
For example, it is stable unless large forces are applied that would destroy it, and it can withstand blood treatment. Therefore, methods of fixing fibers such as air blowing, high pressure steam blowing, etc., which do not completely fix the fibers, but simply entangle the fibers with each other, can also be used as the method of fixing the nonwoven fabric of the present invention.

The bulk density of the denatured blood component removal filter of the present invention is in the range of 0.05 to 0.45 g/ ^cm2 , preferably 0.10 g/cm2.
~0.40g/ ^cm2 . When the bulk density is less than 0.05 g/cm ² , the gaps between the fibers become large and the aggregated substances cannot be completely removed. Also,
When the bulk density is greater than 0.45 g/cm ² , the gaps between the fibers become dense and white blood cells are also captured.

The thickness of the denatured blood component removal filter of the present invention is
In the range of 0.05~0.35mm, preferably 0.10~
The range is 0.30mm. When the thickness of the denatured blood component removal filter is thinner than 0.05 mm, it becomes difficult to completely capture aggregated substances. Moreover, if it becomes thicker than 0.35 mm, the processing speed becomes slow and the recovery rate of white blood cells decreases.

Many denatured blood components in blood or blood cell suspension occur when blood is stored, and the amount and size of aggregated substances vary depending on the length of the storage period. The area required for the component removal filter varies, but the total surface area on the blood inlet side including the gaps between fibers (hereinafter referred to as filter area) is from 15 cm ² / 500 ml of stored blood.
Must be in the range of 2000cm ² /500ml of stored blood, preferably 20cm ² /500ml of stored blood to 1000cm ² /500ml of stored blood
More preferably, the area is between 30 cm ² /500 ml of stored blood and 500 cm ² /500 ml of stored blood.
When the filter area is smaller than 15 cm ² /500 ml of stored blood, the surface of the denatured blood component removal filter becomes saturated with denatured blood components, and the processing speed becomes slow. Also, the filter area is 2000cm ² /
If the stored blood exceeds 500ml, a large amount of physiological saline will be required to collect blood cells in the filter after denatured blood component removal processing, and more blood cells will remain in the denatured blood component removal filter.
The recovery rate of white blood cells and red blood cells decreases.

According to the present invention, the speed at which the blood or blood cell suspension is passed through the filter can be high, but it varies depending on the storage period of the collected blood and the area of the filter on the blood inlet side, and is generally 25
~140ml/min, preferably 30-110
ml/min, more preferably in the range of about 70-100 ml/min.

In order to achieve the purpose of the present invention, that is, to remove everything from large aggregates of denatured blood components to small aggregates from a large amount of blood or blood cell suspension in a short time by high-speed processing with simple operations. , using thin fibers with a diameter of 3 to 10 μm and a bulk density of 0.05 to 0.45 g/cm ² and fixed by entangling the fibers with each other, making the gap between the fibers extremely small, making it possible to reduce red blood cells and white blood cells. The thickness of the non-woven fabric is reduced to 0.05 to capture denatured blood components.
By setting the diameter to ~0.35 mm, the pressure drop of the processing liquid can be reduced and the flow rate can be increased.

DESCRIPTION OF THE PREFERRED EMBODIMENTS The details of the denatured blood component removal filter of the present invention and a filter device using the filter will be explained below with reference to the drawings.

1 and 2 show one embodiment of a filter device using the denatured blood component removal filter of the present invention, and FIGS. 3 and 4 show another embodiment. In FIGS. 1 and 2, 1 is a filter device main body, and two round tray-shaped frames 2 and 2' are fitted with a ring member 3 so as to form a cavity. On the inside of 2,2′,
A large number of protrusions 4, 4' are formed respectively, and a filter 6 made of non-woven fabric is provided inside the protrusions 4, 4' sandwiched between mesh-like supporting members 5, 5'. 7
is a blood inflow pipe provided in one round tray-shaped frame 2,
8 is a blood outflow pipe provided in the other round tray-shaped frame 2', and 9 and 9' are packings. and,
The filter 6 is held by mesh-like supports 5, 5'.

The filter devices shown in FIGS. 3 and 4 are also substantially similar to the filter device described above, except that the blood inflow pipe 13 and the blood outflow pipe 14 are connected to the round tray-shaped frames 10 and 1.
0' in the center, and in the cavity,
A filter 12 made of non-woven fabric is provided sandwiched between mesh-like supports 11, 11'. 1
5, 15' is packing.

Blood or blood cell suspension is introduced from the blood inflow pipe 7 or 13, and passed through the denatured blood component removal filter 6 in the mesh-like support material 5 or 11.
Alternatively, it spreads over the entire surface of 12, flows uniformly into the denatured blood component removal filter, captures and removes denatured blood components, passes through the mesh-like support material 5' or 11', and is collected from the blood outflow tube 8 or 14. become.

FIG. 5 shows one mode of use of the filter device using the denatured blood component removal filter of the present invention.

The blood or blood cell suspension is passed through a circuit 17 from the blood collection bag 16 by the differential pressure, and is passed through a filter device 18 using the denatured blood component removal filter of the present invention.
supplied to The denatured blood components are captured by the denatured blood component removal filter section, and the blood or blood cell suspension from which the denatured blood components have been removed passes through a circuit 19 and is collected into a collection bag 20. In order to increase the recovery rate of red blood cells, white blood cells, etc., from a physiological saline bag 21 prepared in advance, the circuit 22,
Denatured blood component removal filter device 18, circuit 1
9. Physiological saline is poured into the collection bag 20, and the blood or blood cell suspension remaining in the circuit and the denatured blood component removal filter is replaced with physiological saline and collected. 23 is a collection bag, and 24 is a regulating valve. Incidentally, the bulk density of the nonwoven fabric of the present invention refers to the value measured by the weight per 1 cm ² of a uniform nonwoven fabric.

As described above, the filter for removing denatured blood components according to the present invention can completely remove large aggregates to 15-20 μm aggregates from denatured blood components in blood or blood cell suspensions in a short processing time. It's easy to do.

Examples will be described below.

Example 1 A nonwoven fabric with a diameter of 110 mm and a thickness of polyester fibers of 6.4 μm in diameter and a bulk density of 0.35 g/cm ² made by a melt-blowing method was fixed in place by the mutual entanglement of the fibers. Cut into a 0.20 mm cylinder, with an effective inner diameter of 100 mm (effective inner diameter = diameter of the part where blood or blood cell suspension actually contacts the filter surface; the same applies to the following examples), and a thickness of the inner space. It was fixed in a 5 mm column. The total surface area on the blood inlet side of the resulting denatured blood component removal filter was 98 cm ² /500 ml of stored blood. Attach the above-mentioned denatured blood component removal filter device at a height of 800 mm from the two 300 ml blood collection bags, and then attach one infusion bag 800 mm below, which serves as a collection bag to store the processed fluid, and between each A processing device was created that was connected by tubes with an inner diameter of 3 mm and an outer diameter of 5 mm.

In two blood collection bags of this processing device, CPD with a hematocrit of 36% from a healthy type A patient was stored for 14 days.
200 ml of whole blood was added to each bag, and denatured blood components were removed one by one at a room temperature of 25° C. using a gravity drop method using a drop.

Next, add 40ml of physiological saline using the gravity drop method.
The blood was passed through this filter device for removing denatured blood components, and the blood cells in the filter device were collected.

As a result, the processing time for 400ml of this stored blood was 4
The processing time was 95 ml/min, which was a high-speed flow. In addition, the aggregated substances of denatured blood components in the blood stored for 14 days were 15 μm or more before treatment, and the average of 2 bags was 1.65×10 ⁶ particles/ml, but after treatment, it was 0 particles/ml. Furthermore, the leukocyte recovery rate was 91.4% and the red blood cell recovery rate was 92.3%.

Comparative Example 1 Polyester fibers with a diameter of 13 μm and a length of 40 to 70 mm were placed in a cylindrical column with an inner diameter of 100 mm and a thickness of 35 mm, with a space of 5 mm thick at the blood inflow part. Denatured blood component removal filter device (bulk density
0.20g/cm ² ). The total surface area on the blood inlet side of the obtained denatured blood component removal filter was 98 cm ² /
500ml - stored blood. Attach the above-mentioned denatured blood component removal filter device at a height of 800 mm from two 300 ml blood collection bags, and add another 800 mm.
A processing device was constructed in which an infusion bag was attached to the bottom to serve as a collection bag for storing treated blood, and the bag was connected with a tube having an inner diameter of 3 mm and an outer diameter of 5 mm.

In two blood collection bags of this processing device, CPD with a hematocrit of 36% from a healthy type A patient was stored for 14 days.
200 ml of whole blood was added to each bag, and denatured blood components were removed one by one at a room temperature of 25° C. using a gravity drop method using a drop.

Next, add 257 ml of physiological saline using the gravity method.
The blood was passed through this filter device for removing denatured blood components, and the blood cells in the filter device were collected.

As a result, the processing time for 400ml of this stored blood is 21
The processing time was 19 ml/min. In addition, the aggregated substances of denatured blood components in the blood stored for 14 days were 15 μm or more before treatment, and the average of 2 bags was 1.48 × 10 ⁶ /ml, but after treatment, it was 0.51
¹⁰⁶ particles/ml (removal rate of aggregated substances 65.5%).
Furthermore, the recovery rate of white blood cells was 84.8% and the recovery rate of red blood cells was 89.7%.

According to Example 1, the denatured blood component removal filter of the present invention has a processing speed that is about 5 times faster than that of the conventional denatured blood component removal filter, and the denatured blood component removal performance is also higher than that of the conventional method. It was very good.

Example 2 When manufacturing a nonwoven fabric with a bulk density of 0.18 g/cm ² made by melt-blowing polyester fibers with a diameter of 4.6 μm, dry heat treatment was performed at 250°C for 2 seconds during melt-blowing.
A nonwoven fabric was created by thermally fixing the intertwined contact points of fibers. This nonwoven fabric has a diameter of 78 mm and a thickness of 0.25 mm.
It was cut into cylindrical pieces with a diameter of 68 mm and fixed in a column with an effective inner diameter of 68 mm and an internal space thickness of 5 mm. The total surface area on the blood inlet side of the resulting denatured blood component removal filter was 45 cm ² /500 ml of stored blood. Attach the above-mentioned denatured blood component removal filter device at a height of 800 mm from two 300 ml blood collection bags, and then attach one infusion bag below 800 mm to serve as a collection bag for storing the processed fluid, and between each A processing device was created that was connected by tubes with an inner diameter of 3 mm and an outer diameter of 5 mm.

ACD with a hematocrit of 45% from a healthy blood type B patient stored for 8 days was stored in two blood collection bags of this processing device.
- Blood added with liquid A 200 ml of whole blood was added to each bag, and denatured blood components were treated one by one at a room temperature of 25° C. using a gravity drop method using a drop.

Next, add 30ml of physiological saline using the gravity drop method.
The blood was passed through this filter device for removing denatured blood components, and the blood cells in the filter device were collected.

As a result, the processing time for 400ml of this stored blood was 3
The processing time was 101 ml/min, which was a high flow rate. In addition, if the agglutinated substances of the denatured blood components of the blood stored for these 8 days were 15 μm or more before treatment, 2
There was an average of 0.91×10 ⁶ cells/ml,
After treatment: 18,700 particles/ml (removal rate of aggregated substances: 97.9)
%), and the leukocyte recovery rate was 93.2% and the red blood cell recovery rate was 96.1%.

Comparative Example 2 Polyamide fibers (nylon 66) with a diameter of 18 μm and a length of 40 to 70 mm were made into a cylindrical column with an inner diameter of 68 mm and a thickness of 30 mm, with a space of 5 mm thick at the blood inflow part. A denatured blood component removal filter device (bulk density: 0.30 g/cm ² ) was prepared by uniformly packing 27 g into a 68 mm and 25 mm thick section. The total surface area of the obtained denatured blood component removal filter on the blood inlet side is 45
cm ² /500ml - stored blood. Attach the above denatured blood component removal filter device at a height of 800 mm from two 300 ml blood collection bags, and
A processing device was constructed in which an infusion bag was attached 800 mm below the bag to serve as a collection bag for storing treated blood, and the bag was connected with a tube having an inner diameter of 3 mm and an outer diameter of 5 mm.

ACD with a hematocrit of 45% from a healthy blood type B patient stored for 8 days was stored in two blood collection bags of this processing device.
-Blood containing A solution 200 ml of whole blood was added to each bag, and denatured blood components were removed one by one bag by bag at a room temperature of 25° C. using a gravity drop method using a drop.

Next, add 120ml of physiological saline using the gravity drop method.
The blood was passed through this filter device for removing denatured blood components, and the blood cells in the filter device were collected.

As a result, the processing time for 400ml of this stored blood is 22
The processing time was 13 minutes and 13 seconds, and the processing speed was 18 ml/minute. In addition, the aggregated substances of denatured blood components in blood stored for 8 days were 15 μm or more before treatment, and the average of 2 bags was
There were 0.82×10 ⁶ cells/ml, but after treatment it was 0.23×
¹⁰⁶ particles/ml (removal rate of aggregated substances 72.0%).
Furthermore, the recovery rate of white blood cells was 81.2% and the recovery rate of red blood cells was 85.9%.

According to Example 2, the denatured blood component removal filter of the present invention has a processing speed approximately 6 times faster than the conventional denatured blood component removal filter, and the denatured blood component removal performance is also higher than that of the conventional method. It was very good.

Example 3 Polyamide fiber (nylon 66) with a diameter of 5.2 μm
Bulk density 0.26g/cm ² made by melt blowing method
A nonwoven fabric with a diameter of 170 mm and a thickness of
It was cut into a cylindrical shape of 0.20 mm and fixed in a column with an effective inner diameter of 160 mm and an internal space thickness of 5 mm. The total surface area on the blood inlet side of the resulting denatured blood component removal filter was 201 cm ² /500 ml of stored blood.
Attach the above-mentioned denatured blood component removal filters at a height of 800 mm from the two 300 ml blood collection bags, and then attach one infusion bag 800 mm below to serve as a collection bag for storing the processed fluid. A processing device was created that was connected with tubes of 3 mm and outer diameter of 5 mm.

Two blood collection bags of this processing device contain CPD containing a hematocrit of 47% from a healthy type O person for 21 days.
Added whole blood (250 ml each) was added, and the stored blood kept cool at 4°C was taken out using a gravity drop method using a drop, and the denatured blood components were immediately removed one by one at a room temperature of 10°C.

Next, add 110ml of physiological saline using the gravity drop method.
The blood was passed through this denatured blood component removal filter, and the blood cells in the filter were collected.

As a result, the processing time for 500ml of this stored blood was 6
The processing time was 80 ml/min, which was a high flow rate. In addition, the aggregated substances of denatured blood components in the blood stored for 21 days were 15 μm or more before treatment, and the average of 2 bags was 3.25 × 10 ⁶ /ml, but after treatment, it was 84,500 aggregated substances / Removal rate 97.4%)
It was hot. Furthermore, the recovery rate of white blood cells was 89.6% and the recovery rate of red blood cells was 89.7%.

Example 4 A nonwoven fabric with a diameter of 210 mm ^and a thickness of Cut into a cylindrical shape with a diameter of 0.30 mm, an effective inner diameter of 200 mm, and an inner space thickness of 5 mm.
was fixed in a column. The total surface area of the obtained denatured blood component removal filter on the blood inlet side was 449 cm ² /
500ml - stored blood. Attach a denatured blood component removal filter above two 200 ml blood collection bags at a height of 800 mm from each other, and attach one infusion bag 800 mm below to serve as a collection bag for storing the processed fluid. 3
A processing device was created that was connected with a tube with an outer diameter of 5 mm.

Two blood collection bags of this processing device are filled with a blood concentrate containing CPD solution (from which a portion of the plasma has been removed by centrifugation) of a healthy person with type A blood and a hematocrit of 64%.
175 ml of blood stored for 10 days was added to each bag, and denatured blood components were removed one by one at a room temperature of 25°C using a gravity drop method using a drop.

Next, add 160ml of physiological saline using the gravity drop method.
The blood was passed through this denatured blood component removal filter, and the blood cells in the filter were collected.

As a result, the processing time for 350ml of this stored blood was 4
The processing time was 85 ml/min, which was a high flow rate. In addition, if the agglutinated substances of denatured blood components in the concentrated blood stored for the past 10 days are 15 μm or more before processing,
The average of 2 batches was 0.78×10 ⁶ pieces/ml,
After treatment: 41,300 pieces/ml (removal rate of aggregated substances: 94.7%)
It was hot. Furthermore, the leukocyte recovery rate was 85.2% and the red blood cell recovery rate was 87.8%.

Example 5 A nonwoven fabric with a diameter of 90 mm ^and a thickness of It was cut into a cylindrical shape with a diameter of 0.15 mm, and this was used as the present filter.

Next, a heat-fixed nonwoven fabric made of polyester fibers with a diameter of 14 μm and a bulk density of 0.25 g/cm ² was heated to a diameter of 90 mm.
It was cut into a cylindrical shape with a thickness of 0.60 mm, and this was used as the first layer filter to remove large denatured blood components. This first layer filter is stacked on top of the main filter above, and the effective inner diameter is 80 mm and the thickness of the internal space is 6 mm.
was fixed in a column. The total surface area on the blood inlet side of the obtained denatured blood component removal filter was 63 cm ² /
500ml - stored blood. Attach the above-mentioned denatured blood component removal filter device at a height of 800 mm from two 200 ml blood collection bags, and then
A treatment device was constructed by attaching an infusion bag of 1 mm below the bag and using it as a collection bag to store the treated liquid, and connecting the bag with a tube having an inner diameter of 3 mm and an outer diameter of 5 mm.

In the two blood collection bags of this processing device, both
Add 200 ml of CPD solution-added whole blood stored for 21 days from a healthy person with blood type AB and a hematocrit of 43%, remove the stored blood kept cool at 4°C using the gravity drop method, and immediately transfer to room temperature of 10°C. The denatured blood components were removed one by one, one bag at a time.

Next, add 40ml of physiological saline using the gravity drop method.
The blood was passed through this denatured blood component removal filter, and the blood cells in the filter were collected.

As a result, the processing time for 400ml of this stored blood was 5
The processing time was 74 ml/min, which was a high flow rate. In addition, the aggregated substances of denatured blood components in the blood stored for 21 days were ¹⁵ μm or more before treatment, and the average of 2 bags was 3.02 Removal rate 98.2%)
It was hot. Furthermore, the recovery rate for white blood cells was 90.8% and the recovery rate for red blood cells was 91.5%.

Comparative Example 3 A nonwoven fabric with a diameter of 110 mm and a thickness of polyester fibers of 6.4 μm in diameter and a bulk density of 0.35 g/cm ³ made by a melt-blowing method was fixed in place by the mutual entanglement of the fibers. It was cut into a cylindrical shape of 0.45 mm and fixed in a column with an effective inner diameter of 100 mm and an inner space thickness of 5 mm. The total surface area on the blood inlet side of the resulting denatured blood component removal filter was 98cm ² /500
ml - stored blood. Attach the above-mentioned denatured blood component removal filter device at a height of 800 mm from the two 300 ml blood collection bags, and also attach one infusion bag 800 mm below, which serves as a collection bag to store the treated fluid, and between each Inner diameter 3
A processing device was created that was connected with a tube with an outer diameter of 5 mm.

The CPD of a healthy person with type B blood, which had a hematocrit of 38%, was stored for 14 days in two blood collection bags of this processing device.
Added whole blood 200 ml of whole blood was added to each bag, and denatured blood components were removed one by one at room temperature of 25° C. using a gravity drop method using a drop.

Next, add 40ml of physiological saline using the gravity drop method.
The blood was passed through this filter device for removing denatured blood components, and the blood cells in the filter device were collected.

As a result, the processing time for 400ml of this stored blood is 18
The processing time was 11 minutes and 11 seconds, and the processing speed was 22 ml/min. In addition, the aggregated substances of denatured blood components in the blood stored for 14 days were 15 μm or larger before treatment, and the average of 2 bags was 1.84×10 ⁶ particles/ml, but after treatment, it was 0 particles/ml. However, the leukocyte recovery rate was 43.1% and the red blood cell recovery rate was 90.7%.

Comparative Example 4 A nonwoven fabric with a bulk density of 0.35 g/cm ³ made of polyester fibers with a diameter of 14 μm by melt-blowing and fixed in position by the mutual entanglement of the fibers was fabricated with a diameter of 78 mm and a thickness of 0.30 μm. It was cut into a cylindrical shape of mm and fixed in a column with an effective inner diameter of 100 mm and an internal space thickness of 5 mm. The total surface area of the obtained denatured blood component removal filter on the blood inlet side is 45cm ² /500
ml - stored blood.

800mm head from two 300ml blood collection bags each
Attach the above-mentioned denatured blood component removal filter device at the position, and also attach an infusion bag 1 800 mm below to serve as a collection bag to store the treated fluid, and connect them with a tube with an inner diameter of 3 mm and an outer diameter of 5 mm. A processing device was created.

A hematocrit of 42% from a healthy AB type patient was stored for 8 days in two blood collection bags of this processing device.
200 ml of CPD solution-added whole blood was added to each bag, and denatured blood components were removed one by one at a room temperature of 25° C. using a gravity drop method using a drop.

Next, add 40ml of physiological saline using the gravity drop method.
The blood was passed through this filter device for removing denatured blood components, and the blood cells in the filter device were collected.

As a result, the processing time for 400ml of this stored blood was 3
The processing time was 105 ml/min, which was a high-speed flow. However, the agglutinants of denatured blood components in the blood stored for these 8 days were larger than 15 μm before treatment.
The average number of 2 bags was 1.29×10 ⁶ cells/ml, but even after treatment, 0.423×10 ⁶ cells/ml remained. The white blood cell recovery rate was 88.2% and the red blood cell recovery rate was 91.8.
It was %.

[Brief explanation of the drawing]

FIG. 1 is a front view showing one embodiment of a filter device using the denatured blood component removal filter of the present invention, FIG. 2 is a longitudinal sectional side view of the same, FIG. 3 is a front view showing another embodiment, and FIG. The longitudinal side view and FIG. 5 are explanatory diagrams showing how the filter device using the denatured blood component removal filter of the present invention is used. 1... Filter device main body, 2, 2'... Round tray-shaped frame body, 3... Ring member, 4, 4'... Projection,
5, 5'...Mesh-shaped support material, 6...Filter, 7...Blood inflow pipe, 8...Blood outflow pipe, 9,
9'... Packing, 10, 10'... Round tray-shaped frame, 11, 11'... Mesh-shaped support material, 12...
...Filter, 13...Blood inflow pipe, 14...Blood outflow pipe, 15, 15'...Packing, 16...
...Blood collection bag, 17...Circuit, 18...Filter device, 19...Circuit, 20...Collection bag, 2
1...Physiological saline bag, 22...Circuit, 23...
...Recovery bag, 24...Adjustment valve.

Claims (1)

[Claims] 1 Fiber diameter is 3 to 10 μm, bulk density is 0.05 to
0.45g/ ^cm3 , made of non-woven fabric fixed by intertwining of fibers, its thickness is 0.05~0.35mm, and its total surface area is 15~ ^2000cm2 /500ml
- A denatured blood component removal filter for passing leukocytes and capturing only denatured blood components, which is characterized by being stored blood.