JPH0581237B2 - - Google Patents

Info

Publication number
JPH0581237B2
JPH0581237B2 JP63173761A JP17376188A JPH0581237B2 JP H0581237 B2 JPH0581237 B2 JP H0581237B2 JP 63173761 A JP63173761 A JP 63173761A JP 17376188 A JP17376188 A JP 17376188A JP H0581237 B2 JPH0581237 B2 JP H0581237B2
Authority
JP
Japan
Prior art keywords
strain
plasmid
yeast
pαly1
reductase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP63173761A
Other languages
English (en)
Japanese (ja)
Other versions
JPH0223870A (ja
Inventor
Megumi Shibata
Toshuki Sakaki
Yoshasu Yabusaki
Hiroko Murakami
Hideo Ookawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology AIST
Original Assignee
Agency of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology
Priority to JP63173761A priority Critical patent/JPH0223870A/ja
Publication of JPH0223870A publication Critical patent/JPH0223870A/ja
Publication of JPH0581237B2 publication Critical patent/JPH0581237B2/ja
Granted legal-status Critical Current

Links

Classifications

    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/52Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP63173761A 1988-07-14 1988-07-14 チトクロムP450↓1↓7αと酵母NADPH‐チトクロムP450還元酵素の融合酸化酵素 Granted JPH0223870A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP63173761A JPH0223870A (ja) 1988-07-14 1988-07-14 チトクロムP450↓1↓7αと酵母NADPH‐チトクロムP450還元酵素の融合酸化酵素

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP63173761A JPH0223870A (ja) 1988-07-14 1988-07-14 チトクロムP450↓1↓7αと酵母NADPH‐チトクロムP450還元酵素の融合酸化酵素

Publications (2)

Publication Number Publication Date
JPH0223870A JPH0223870A (ja) 1990-01-26
JPH0581237B2 true JPH0581237B2 (de) 1993-11-11

Family

ID=15966655

Family Applications (1)

Application Number Title Priority Date Filing Date
JP63173761A Granted JPH0223870A (ja) 1988-07-14 1988-07-14 チトクロムP450↓1↓7αと酵母NADPH‐チトクロムP450還元酵素の融合酸化酵素

Country Status (1)

Country Link
JP (1) JPH0223870A (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2128399A1 (en) 1993-07-20 1995-01-21 Koji Hayashi Method for safety evaluation of chemical compound using recombinant yeast expressing human cytochrome p450

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JOURNAL OF BIOCHEMISTRY=1988 *
JOURNAL OF BIOLOGICAL CHEMISTRY=1986 *

Also Published As

Publication number Publication date
JPH0223870A (ja) 1990-01-26

Similar Documents

Publication Publication Date Title
US7262038B2 (en) Simplified eukaryotic nitrate reductase
JP3283551B2 (ja) 酵母中でスクアレンおよび特異的なステロールの蓄積を増加させるための方法と組成物
KR101861387B1 (ko) 효모에 의한 비효모 스테롤의 제조
US5114852A (en) Chimeric fused monooxygenase of cytochrome P-450 and NADPH-cytochrome P-450 reductase
EP0477961B1 (de) Mitochondriales P450
US5137822A (en) Transformed yeasts having steroid-hydroxylase activity and process for hydroxylation of steroids using the said transformed yeasts
DK175573B1 (da) Anvendelse af en ekspressionskassette förende til biokemisk oxidation af steroider, sådanne ekspressionskassetter,
Popenoe et al. Partial purification and properties of collagen lysine hydroxylase from chick embryos
EP0360361A1 (de) Verfahren zur mehrfachen Oxidation von Steroiden und dafür verwendbare, genetisch manipulierte Zellen
JPH0581237B2 (de)
JP2517894B2 (ja) フェレドキシン及びフェレドキシン還元酵素を同時に発現させるキメラp450産生菌株
CN116875571A (zh) 一种p450酶突变体、单质粒三酶共表达系统及其在骨化二醇合成中的应用
JP2500543B2 (ja) キメラp450産生菌株
JPS62104582A (ja) ラット肝チトクロムp―450cおよびnadph―チトクロムp―450還元酵素を同時に酵母内で発現させることを目的とした発現用プラスミドparm1,ならびにparm1を菌体内に保持する酵母菌体
JPH02249488A (ja) チトクロムP450↓c↓2↓1と酵母NADPH‐チトクロムP450還元酵素の融合酸化酵素、該酵素をコードする遺伝子および該酵素の製造方法
JP3387525B2 (ja) チトクロムP450cと酵母NADPH−チトクロムP450還元酵素の融合酸化酵素、該酵素をコードする遺伝子および該酵素の製造方法
JP3577722B2 (ja) 人工融合酵素
JPH0569511B2 (de)
JPH0429352B2 (de)
JPH04135486A (ja) キメラp450発現プラスミド及び該プラスミドを保持する酵母菌株
JPH07147975A (ja) 人工融合酵素
JPH0231680A (ja) チトクロムP450c↓2↓1産生酵母菌株
CN121975899A (en) Method for synthesizing dehydroepiandrosterone by chemical-multienzyme coupling catalysis
CN119639694A (zh) 蛋白质组合及其在生产甾体化合物中的应用
CN117025709A (zh) 一种细胞色素p450酶联合细胞色素p450还原酶在合成熊去氧胆酸中的应用

Legal Events

Date Code Title Description
EXPY Cancellation because of completion of term