JPH05962A - Mugwort extract and its use - Google Patents

Abstract

PURPOSE:To provide the subject extract of high stability, causing only little clothing discoloration, having excellent efficacy on wound therapies, skin chapping prevention, skin chapping improvement, and dermal pruritus, body smell and foul breath prevention. CONSTITUTION:Mugwort is either refluxed under heating with water or extracted with an organic solvent, or immersed in water or in the solvent, followed by filtration to obtain a mugwort crude extract, which is, in turn, put to e.g. gel permeation chromatography, and the initial aqueous effluent corresponding to a holding time of 0-50min is virtually removed, thus obtaining the objective mugwort extract with an average molecular weight of 15000 after eliminating protein component. The present extract is used for cosmetics, detergent compositions, compositions for oral cavity and foods as formulation containing the extract 0.00001-5wt.%.

Description

Detailed Description of the Invention

[0001]

The present invention relates to a mugwort extract having good stability and little coloration on clothes, and containing it to prevent wound healing, prevent rough skin, and improve rough skin, as well as skin itch, body odor and bad breath. The present invention relates to a cosmetic, a cleaning composition, an oral composition and a food which are excellent in the effect of preventing the above.

[0002]

2. Description of the Related Art Conventionally, extracts of water or hydrous alcohol of mugwort have been blended in various compositions for the purpose of healing wounds, preventing rough skin, improving rough skin, and preventing itching of skin, body odor and bad breath. It has been. However, the extract of water or hydrous alcohol of mugwort originally has a dark brown to dark brown color.For example, when it is used as it is in cosmetics, this color adheres to clothes, and when the cosmetic containing it is stored for a long time, There were problems in stability such as browning and precipitation. So far, activated carbon treatment has been taken to solve this problem, but the disadvantages such as wound healing, prevention of rough skin, improvement of rough skin, itching of the skin, prevention of body odor and bad breath are also lost. was there. In addition, the extract obtained from the beginning by using an organic solvent such as hexane, ethyl acetate, acetone, ethanol, and methanol contained terpenes and dark chlorophyll, which have safety problems, and was problematic in use. These effects were still unsatisfactory.

[0003]

DISCLOSURE OF THE INVENTION Problems to be Solved by the Invention In view of these circumstances, the present inventors have conducted extensive studies and as a result, found that the average molecular weight was 1
It was found that 5000 proteins were the cause of browning and precipitation of mugwort extract, and the mugwort extract that had substantially removed it had significantly better stability than the original extract and less coloring on clothes, In addition to wound healing, prevention of rough skin, and improvement of rough skin, the inventors have found that they are also excellent in the effects of preventing itching of skin, body odor and bad breath, and have completed the present invention.

[0004]

[Means for Solving the Problems] That is, the present invention is
A mugwort extract in which a protein having an average molecular weight of 15,000 is substantially removed. And a cosmetic, a detergent composition, a composition for the oral cavity, and a food which are characterized by containing the mugwort extract. Hereinafter, the present invention will be described in detail.

The mugwort extract of the present invention can be obtained, for example, by the following method. Mugwort is heated or refluxed or immersed with water or one or more kinds of water-containing alcohols such as water-containing methanol, water-containing ethanol, water-containing butanol, water-containing propanol, water-containing 1,3-butylene glycol and water-containing glycerin, and filtered to make the mugwort. A crude extract is obtained. At this time, in order to remove the hydrophobic component, it may be extracted in advance with a nonpolar solvent such as hexane.

Next, the water or water-containing ethanol extract of mugwort thus obtained is treated by, for example, gel permeation chromatography set under the following conditions, and the initial water outflow for a retention time of 0 to 25 minutes is substantially reduced. By removing it selectively, it is possible to obtain a mugwort extract with good stability and little coloring on clothes.

Column: Rover column inner diameter 3 cm × length 30 cm Filler: cross-linked dextran oxypropyl ether gel (for example, Sephadex LH-20 manufactured by Pharmacia KK Column temperature: room temperature Solvent: water 200 ml → 70 % Acetone 100 ml Flow rate: 2.0 ml / min Sample amount: Wormwood hot water extract 5 ml Detection: Wavelength 254 nm

The gel permeation chromatography can also be obtained by treating the gel permeation chromatography under the following conditions to substantially remove the initial water outflow for a retention time of 0 to 50 minutes.

Column: packed column for high performance liquid chromatography 7.5 mm inner diameter × 30 cm length Filler: hydrophilic silica gel (eg Tosoh Corp.)
Manufactured by TSK gel G3000SW) Solvent: 0.2M phosphate buffer Flow rate: 0.5 ml / min Sample amount: 100 μl Detection: Wavelength 280 nm

FIG. 1 shows a chromatogram in which a protein having an average molecular weight of 15,000 contained in the water or hydrous alcoholic extract of mugwort was detected by gel permeation chromatography set under the above conditions.

Further, the same qualitative low sensitizing mugwort extract can be obtained by treating the water or hot water extract of mugwort as follows. That is, the water or hot water extract of mugwort is re-extracted with 50% to 100% of water-containing ethanol, water-containing methanol, water-containing butanol, water-containing 1,3-butylene glycol, water-containing alcohol such as glycerin. Then, the insoluble portion is obtained by filtration and removal. Alternatively, on the contrary, it can also be obtained by first extracting with a polar organic solvent such as ethyl acetate, acetone, ethanol or methanol, re-extracting this extract with water or hot water, and filtering and removing the insoluble portion. To be
Also in this case, in order to remove the hydrophobic component, it may be extracted in advance with a nonpolar solvent such as hexane.

The content of the mugwort extract in the cosmetic according to the present invention is 0.00001 to 5 as a dry product in the total amount of the cosmetic.
%, Preferably 0.0005 to 1%. If it is less than 0.00001%, the effect of the present invention is not sufficiently exhibited, which is not preferable.

The amount of mugwort extract in the detergent composition according to the present invention is such that it is a dry product in the total amount of the detergent composition.
It is 0.00001 to 5%, preferably 0.000 5 to 1%. 0.0000
If it is less than 1%, the effect of the present invention is not sufficiently exhibited, which is not preferable.

The content of the mugwort extract in the oral composition according to the present invention is such that it is a dry product in the total amount of the oral composition.
It is 0.00001 to 5%, preferably 0.000 5 to 1%. 0.0000
If it is less than 1%, the effect of the present invention is not sufficiently exhibited, which is not preferable.

The content of the mugwort extract in the food according to the present invention is 0.00001 to 5%, preferably 0.0005 to 1% as a dried product in the total amount of the food. When it is less than 0.00001%,
The effect of the present invention is not sufficiently exerted, which is not preferable.

The cosmetics, detergent compositions, oral composition cosmetics, and foods of the present invention may be added to each composition in addition to the above-mentioned essential components, if necessary, within a range that does not impair the effects of the present invention. Various components generally used can be blended.

For example, powder components such as titanium dioxide, mica, talc, and pigments. Avocado oil, macadamia nut oil, corn oil, olive oil, rapeseed oil, evening primrose oil, castor oil, sunflower oil, tea seed oil, rice bran oil, jojoba oil, cacao butter, coconut oil, squalene, squalane, beef tallow, mokuro, beeswax, can Delilla wax, carnauba wax, whale wax, lanolin, liquid paraffin, ceresin,
Oils such as petrolatum, polyoxyethylene (8 mol) oleyl alcohol ether, and glyceryl monooleate. Capryl alcohol, lauryl alcohol, myristyl alcohol, cetyl alcohol, cholesterol,
Higher alcohols such as phytosterols, capric acid,
Higher fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, lanolin fatty acid, linoleic acid and linolenic acid. Para-aminobenzoic acid, homomenthyl-7N-acetylanthraniate, butylmethoxybenzoylmethane, di-paramethoxycinnamic acid-mono-
UV absorbers such as glyceryl 2-ethylhexanoate, amyl salicylate, octyl cinnamate, and 2,4-dihydroxybenzophenone.

Polyethylene glycol, glycerin, sorbitol, xylitol, maltitol, mucopolysaccharide,
Moisturizers such as hyaluronic acid, chondroitin sulfate, chitosan, carboxymethyl chitin (salt). Methyl cellulose, ethyl cellulose, carboxymethyl cellulose,
Thickeners such as gum arabic, polyvinyl alcohol, montmorillonite and saponite. Organic solvents such as ethanol and 1,3-butylene glycol. Antioxidants such as butylhydroxytoluene, tocopherol, phytic acid. Antibacterial preservatives such as benzoic acid, salicylic acid, sorbitan acid, dehydroacetic acid, paraoxybenzoic acid alkyl ester (ethyl paraben, butyl paraben, etc.) hexachlorophene. Glycine, alanine, valine, leucine,
Amino acids such as serine, threonine, phenylalanine, tyrosine, aspartic acid, glutamic acid, asparagine, glutamine, taurine, arginine and histidine, and their alkali metal salts and hydrochlorides. Organic acids such as acyl sarcosine salts (eg sodium lauroylcocin), glutathione, citric acid, malic acid, tartaric acid, lactic acid, vitamin A and its derivatives, vitamin B6 hydrochloride,
Vitamin B6 tripalmitate, vitamin B6 dioctanoate, vitamin B2 and its derivatives, vitamin B1
2, vitamin B15 such as vitamin B15 and its derivatives,
Vitamin Cs such as ascorbic acid, ascorbic acid sulfate, ascorbic acid phosphate, ascorbic acid dipalmitate, α-tocopherol, β-tocopherol, γ-tocopherol, vitamin E asate, vitamin E nicotinate and other vitamin Es, vitamin D , Vitamins such as vitamin H, pantothenic acid, and pantethine. Nicotinic acid amide, benzyl nicotinate, γ-oryzanol, allantoin, glycyrrhizic acid (salt), glycyrrhetinic acid and its derivatives, hinokitiol, mucidin, bisabolol, eucalyptol,
Various drugs such as phytosterol, thymol, inositol, saponins (such as saikosaponin, carrot saponin, hemimasaponin, muclodisaponin) pantothenyl ethyl ether, ethinyl estradiol, cepharanthin, placenta extract.

Licorice, paprika, oguruma, albacore, Rumex, Clara, camphor, camphor, Dokudami, honeysuckle, celery, geranium, turmeric, sardine, orange, sage, euglena, scorpionfish, mistletoe, mallow, senkyu, senburi, senburi, senburi, senburi Clove, cock, spruce, spruce, carrot,
Garlic, Novara, birch, parsley, gentian, mint, fennel, horsetail, saffron, Dutch mustard,
Soap, butcher bloom, grapes, ivy,
Loofah, nettle, bodaiju, hop, salamander,
Shiitake, horse chestnut, honeywort, swordfish, melissa, peach, eucalyptus, dio, shikon, yukinoshita, lily, perilla, peony, rosemary, lemon, ginger, ages, waremokou, birch, raspberry,
Amber, aloe, cucumber, burdock, gardenia,
A natural extract derived from organic solvents, alcohols, polyhydric alcohols, water, hydrous alcohol, etc., such as psyllium, laurel, acacia, yamcha, turmeric, chinobhiba, mackerel, capsicum, butterbur, sarnocaensis, choreimaitake, ganoderma lucidum, and red algae.

Sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, sorbitan sesquioleate, sorbitan trioleate, polyoxyethylene sorbitan monolaurate, polyoxyethylene sorbitan monostearate, polyoxyethylene glycol monooleate, poly Nonionic surfactants such as oxyethylene alkyl ether, polyglycol diester, lauryl diethanol amide, fatty acid diethanol amide, cationic surfactants such as stearyl trimethyl ammonium chloride, benzalkonium chloride, sodium palmitate, sodium laurate, lauryl Sodium sulfate, potassium lauryl sulfate, triethanolamine ether alkylsulfate,
Anionic and amphoteric surfactants such as funnel oil, linear dodecylbenzene sulfate, polyoxyethylene hydrogenated castor oil maleic acid. Examples include fragrance and purified water. Also,
The dosage form of the cosmetic, cleaning composition, oral composition, and food of the present invention is arbitrary, for example, a solubilizing system such as lotion,
An emulsion system such as an emulsion or a cream, or a dosage form such as a dispersion or an ointment can be used.

[0020]

EXAMPLES Next, the effects of the present invention will be described in detail based on examples, but this does not limit the scope of the present invention.

Example 1 500 ml of water was added to 100 g of mugwort and heated for 2 hours in a water bath to obtain an extract, which was treated by gel permeation chromatography under the following conditions. The chromatogram obtained at this time is shown in FIG. By removing the initial water outflow for the retention time of 0 to 25 minutes in FIG.
8.0 g of the desired low-sensitizing mugwort extract was obtained. Column: Rover column inner diameter 3 cm x length 30 cm Filler: Cross-linked dextran oxypropyl ether gel (for example, Sephadex LH-20 manufactured by Pharmacia Co., Ltd.) Column temperature: Room temperature Solvent: Water 200 ml → 70% Acetone 100 ml Flow rate: 2.0 ml / min Sample amount: Mugwort hot water extract 5 ml Detection: Wavelength 254 nm Since the chromatogram of the heated extract is shown in Fig. 2, a protein peak with an average molecular weight of 15,000 can be seen at a retention time of 25 minutes. .

Example 2 To 100 g of mugwort, 1000 ml of water was added, and the mixture was heated in a water bath for 2 hours to concentrate the hot water extract, and then 70% hydrous ethanol 3 was added.
The extract was re-extracted with 00 ml, the insoluble portion was filtered, and then concentrated to obtain 3.3 g of an extract. This was subjected to gel permeation chromatography set under the following conditions, and it was confirmed that the protein having an average molecular weight of 15,000 detected in FIG. 1 was removed. At this time, a chromatogram of the obtained mugwort extract of the present invention is shown in FIG. 3, and a chromatogram of the crude extract that has not been reextracted with 70% ethanol is shown in FIG. In FIG. 4, holding time 4
Although a rising edge of a peak derived from a protein having an average molecular weight of 15,000 is observed at 0 minutes, a rising edge of the peak is observed at a retention time of 50 minutes in FIG. 5, indicating that the above protein has been removed. Column: Packed column packing for high performance liquid chromatography Filler: Hydrophilic silica gel (eg Tosoh Corporation)
TSK gel G3000 SW) manufactured by Solvent: Solvent: 0.2M Phosphate buffer Flow rate: 0.5 ml / min Sample amount: Mugwort hot water extract 100 μl Detection: Wavelength 280 nm

Example 3 800 ml of water was added to 100 g of mugwort and 400 ml of ethanol was added to the extract obtained by immersion extraction for 3 days. The insoluble portion was filtered and then concentrated to obtain 5.4 g of an extract. It was confirmed as follows that the substance having a molecular weight of 15,000 substantially removed in the above Examples was a protein. Figure 5
1 shows the infrared absorption spectrum of the compound having a molecular weight of 15,000 shown in FIG. 1 (bottom row) and the infrared absorption spectrum of the enzyme protein (protease) (top row). The main absorptions of the two are in good agreement, the molecular weight of this compound is as large as 15,000, and this compound reacts with a protein color reagent (for example, a protein assay kit manufactured by Bio-Rad Co., Ltd.). , This compound was determined to be a protein.

Next, in order to compare the stability and color strength of the mugwort extract thus treated with that of the untreated product, each extract was adjusted to a concentration of 1%, and the extract was adjusted to 1 at 50 ° C. It was stored for a month and examined for the presence of precipitation. Also, the wavelength is 440 nm
The absorbance of was measured. As the untreated product, a hot water extract of mugwort, a water immersion extract, a 70% water-containing 1,3-butylene glycol extract, a 70% water-containing ethanol extract,
The test was conducted. Table 1 shows the presence or absence of precipitation after storage for 1 month.
The absorbance at a wavelength of 440 nm is shown in Table 2, respectively.

[0025]

[Table 1] ────────────────────────────                                 Presence of precipitation     Before test After test ────────────────────────────   Example 1 None None   Example 2 None None   Example 3 None None   No hot water extract Gray precipitate   No water-soaked extract Gray precipitate   70% water-containing 1,3-BG extract None Brown precipitate   70% hydrous ethanol extract None Brown precipitate ────────────────────────────

[0026]

[Table 2] ────────────────────────────                                 Presence of precipitation       Before test After test ────────────────────────────   Example 1 0.53 0.67   Example 2 0.72 0.83   Example 3 0.61 0.76   Hot water extract 2.05 3.72   Soaked water extract 1.78 3.87   70% hydrous 1,3-BG extract 1.53 2.53   70% hydrous ethanol extract 2.61 4.91 ────────────────────────────

As shown in Tables 1 and 2, the mugwort extract obtained by the present invention has improved stability and a lighter color than the conventional mugwort extract despite the same concentration. There are few problems such as color adhering to clothes. In addition, in order to show effects such as wound healing, prevention of rough skin, improvement of rough skin, and prevention of itchiness of the skin and body odor, a cell proliferation promoting action and a deodorizing effect of ethyl mercaptan were tested. Next, in order to show the effect of preventing itching of the skin, pruritus senile, etc., a test for the effect of preventing pruritus induced by bradykinin and a practical use test were conducted.

(Cell growth promoting action) Human skin tissue was cut into small pieces and attached to the bottom surface of a petri dish for cell culture.
After culturing in sMEM culture solution (containing 10% fetal bovine serum) for 1 week, the bottom surface of the petri dish is almost entirely filled with fibroblasts. The fibroblasts were treated with 0.25% trypsin solution to give single cells, then 10,000 co-cells / m
Make l cell suspension and add 0.1m of this solution to each dish.
l, Eagle's MEM culture solution and various mugwort extracts (final concentration 1 μl / ml) were further added, and the cells were cultured in a CO 2 incubator for 2 weeks.
Cell colonies were counted. The case where various mugwort extracts were not added served as a control. The cell growth promoting rate is a value obtained by dividing the number of colonies of cells treated with various mugwort extracts by the number of colonies of control cells and multiplying by 100.

Table 3 shows the cell growth promoting rate after culturing for 2 weeks. The determination method is as follows. -Judgment-A: Cell growth promotion rate of 150% or more O: Cell growth promotion rate of 120% to less than 150% B: Cell growth promotion rate of 100% to less than 120% X: Cell growth promotion rate of less than 100%

[0030]

[Table 3]

The extracts obtained in Examples 1 to 3 exhibited a stronger cell growth promoting action than any of the untreated extracts. (Effect of deodorizing ethyl mercaptan) Put various mugwort extracts into a 50 ml screw tube and add 10 m of water.
1 and 10 μl of ethyl mercaptan were added, and 10 minutes later, the amount of ethyl mercaptan existing in the upper space of the screw tube was quantified by headspace gas chromatography. Table 4 shows the residual rate of ethyl mercaptan relative to the blank. The determination method is as follows. -Judgment- A: Residual rate less than 10% O: Residual rate 10% to less than 50% B: Residual rate 50% to less than 80% X: Residual rate 80% or more

[0032]

[Table 4]

As can be seen from Table 4, the extracts obtained in Examples 1 to 3 had a higher deodorizing effect than any of the untreated extracts. (Test for the prevention effect of bradykinin-induced pruritus) Bradykinin 5% solution was applied to the back skin of Hartley albino guinea pigs.
Treated with 0.5 ml. Then, lotions having the basic formulations shown in Table 5 were produced, and 5% by weight of each sample was added to each lotion. The behavior of 10 guinea pigs in 1 group was observed over 3 hours, and the number of times of each behavior was multiplied by the score according to the following criteria to obtain the total score.

[0034]

[Table 5] Lotion basic prescription ───────────────────────── Ingredient amount (% by weight) ───────────────────────── Glycerin 2.0 Propylene Gril 1.0 Citric acid 0.2 95% ethanol 10.0 Fragrance suitable amount POE (20) Lauryl ether 0.5 Purified water residue ─────────────────────────

-Judgment Criteria-Score Evaluation 1 Lick the injection site. 2 Scratch the injection site with your hind legs. Next, the prevention rate (%) of bradykinin-induced pruritus was calculated by the following formula 1
Sought by.

[0036]

[Equation 1]

Table 6 shows the preventive effect on the pruritus induced by bradykinin of each sample. The determination method is as follows. -Determination of Bradykinin-Induced Pruritus Prevention Effect- A: Pruritus prevention rate (%) 75% or more O: Pruritus prevention rate (%) 50% or more and less than 75% △: Pruritus prevention rate (%) 25% or more 50 Less than% x: Pruritus prevention rate (%) less than 25%

[0038]

[Table 6]   Prevention of pruritus induced by bradykinin ───────────────────────────────   Sample Pruritus prevention effect ───────────────────────────────   Lotion only ×   Example 1 blended lotion   Example 2 compounded lotion ◎   Example 3 compounded lotion ◎   Lotion mixed with mugwort water immersion extract △   Mugwort hot water extract combination lotion ○   Lotion containing mugwort 70% water-containing 1,3-BG extract △   Lotion containing mugwort 70% hydrous ethanol extract ○   Lotion containing diphenhydramine hydrochloride ×   Lotion containing adrenal cortex hormone △ ───────────────────────────────

As described above, the strong inhibitory effect on the pruritus induced by bradykinin was confirmed by the lotions of Examples 1, 2 and 3.

(Actual Use Test) The effect of preventing skin itch by the actual use test is shown below. -Test method-For 50 elderly people in an elderly care facility suffering from pruritus senilis, a double-blind lotion of Production Example 2 blended lotion, mugwort hot water extract blended lotion, and placebo basic prescription lotion for 1 day each. The composition was applied twice, and after 2 weeks, the effects were compared by an interview. The evaluation is shown below. Rating Evaluation 1 Itching worsened. 2 Almost the same as before. 3 Itching improved slightly. 4 Itching has improved. 5 I no longer feel itchy. Judgment was performed based on the score according to the following criteria. ◯: Percentage of panels evaluated to be 4 or 5 is 75% or more Δ: Percentage of panels evaluated to be 4 or 5 is 25% or more and less than 75% ×: Percentage of panels evaluated to be 4 or 5 Is less than 25%. The results are shown in Table 7.

[0041]

[Table 8]

As is clear from Tables 6 and 7, the external preparation for skin of the present invention is a novel external preparation for skin which is excellent in the effect of preventing itching of the skin. Next, each composition containing the mugwort extract of the present invention will be described in detail with reference to formulation examples. The present invention is not limited to this. The blending amount is% by weight, and the blending amount of various sensitizing mugwort extracts is described as a dried product.

Example 4 Lotion (1) Wormwood extract obtained in Example 1 1.0 (2) Glycerin 4.0 (3) 1,3-Butylene glycol 4.0 (4) Ethanol 7.0 ( 5) Polyoxyethylene oleyl alcohol 0.5 (6) Methylparaben 0.05 (7) Citric acid 0.01 (8) Sodium citrate 0.1 (9) Perfume 0.05 (10) Purified water Residual (production method) Citric acid, sodium citrate, glycerin, 1,3-butylene glycol, and the mugwort extract obtained in Example 1 are dissolved in purified water. Separately, polyoxyethylene oleyl alcohol, a fragrance, and methylparaben were dissolved in ethanol, and this was added to the above-mentioned purified aqueous solution to solubilize it, followed by filtration to obtain a lotion.

Example 5 Cream (1) cetostearyl alcohol 3.5 (2) squalane 40.0 (3) beeswax 3.0 (4) reduced lanolin 5.0 (5) ethylparaben 0.3 (6) poly Oxyethylene (20) sorbitan monopalmyrtic acid 2.0 ester (7) stearic acid monoglyceride 2.0 (8) Wormwood extract obtained in Example 2 0.005 (9) Perfume 0.03 (10) 1, 3-Butylene glycol 5.0 (11) Glycerin 5.0 (12) Sodium hyaluronate 0.05 (13) Purified water Residue (Production method) (1) to (9) were dissolved by heating and kept at 75 ° C. The ingredients are added to (10) to (13) heated to 75 ° C with stirring. After processing with a homomixer and making the emulsion particles finer,
It was cooled rapidly with stirring to obtain a cream.

Example 6 Emulsion (1) Artemisia extract obtained in Example 3 0.5 (2) Stearic acid 1.5 (3) Cetyl alcohol 0.5 (4) Beeswax 2.0 (5) Polyoxy Ethylene (10) Mono 1.0 Nooleic acid ester (6) Glycerin monostearic acid 1.0 ester (7) Quinceseed extract 20.0 (5% aqueous solution) (8) Propylene glycol 5.0 (9) Ethanol 3 0.0 (10) Ethylparaben 0.3 (11) Perfume 0.03 (12) Purified water To the residue (production method) ethanol, the mugwort extract obtained in Example 3 and the perfume are added and dissolved. (Alcohol phase) Propylene glycol is added to purified water and dissolved by heating to maintain the temperature at 70 ° C.
(Aqueous phase) Other components except the quince seed extract were mixed, dissolved by heating, and kept at 70 ° C. (Oil phase) The oil phase is added to the water phase for preliminary emulsification, and then homogenized with a homomixer. This was cooled to 30 ° C. with stirring to obtain an emulsion.

Example 7 Pack (1) Wormwood extract obtained in Example 1 0.1 (2) Polyvinyl alcohol 15.0 (3) Polyethylene glycol 3.0 (4) Propylene glycol 7.0 (5) Ethanol 10.0 (6) Methylparaben 0.05 (7) Perfume 0.05 (8) Purified water To the residual (manufacturing method) purified water, the mugwort extract obtained in Example 1, polyethylene glycol, propylene glycol and methylparaben were added and stirred. Dissolve. Next, polyvinyl alcohol was added and heated and stirred, and ethanol in which a fragrance was dissolved was added and dissolved by stirring to obtain a pack.

Example 8 Cosmetic for scalp (scalp treatment) (1) Artemisia extract obtained in Example 1.5 1.5 (2) 1,3-butylene glycol 6.5 (3) Polyethylene glycol 1500 5.0 (4) Ethanol 5.5 (5) Potassium hydroxide 0.05 (6) Purified water 45.5 (7) 2-Hexyldecyl palmy 10.0 Tate (8) Squalane 5.0 (9) Butyl paraben 0.0. 2 (10) Vitamin C 0.15 (11) Perfume 0.05 (12) Purified water 19.9 (13) Carboxyvinyl polymer 0.2 (Production method) (7) to (11) were dissolved at 75 ° C. (1) to (4) and (6) kept at 75 ° C with stirring,
Further, (5), (12) and (13) dissolved under stirring at room temperature were added and cooled with stirring to obtain a scalp treatment.

Example 9 Ointment (1) Artemisia extract obtained in Example 3 5.0 (2) Stearyl alcohol 18.0 (3) Mokurou 20.0 (4) Polyoxyethylene (10) 0.25 Mono Oleic acid ester (5) Glycerin monostearic acid 0.25 Ester (6) Vaseline 40.0 (7) Purified water 16.5 (Production method) Purified water is kept at 70 ° C, and (water phase) other components are added to 70%. Mix and dissolve at ° C. (Oil phase) The oil phase was added to the aqueous phase, the mixture was homogenized with a homomixer, and then cooled to obtain an ointment.

Example 10 Toothpaste (1) Silicic anhydride 20.0 (2) Solbit 50.0 (3) Carrageenan 0.5 (4) Mugwort extract obtained in Example 1 1.2 (5) Carboxymethyl cellulose 1.0 sodium (6) Sodium lauryl sulfate 1.8 (7) Saccharin sodium 0.08 (8) Methyl parahydroxybenzoate 0.2 (9) Fragrance 0.9 (10) Purified water Residual The above toothpaste was obtained by a conventional method.

Example 11 Shampoo (1) Lauryl sulfate triethanolamine 15.0 (2) Coconut oil fatty acid monoethanol amide 5.0 (3) Mugwort extract obtained in Example 2 0.2 (4) Fragrance 0.9 (5) Purified water Residual Shampoo was obtained by a conventional method.

Example 12 A chewing gum gum mixer was charged with 29.5% by weight of gum base, 10.0% by weight of starch syrup and 60.0% by weight of powdered sugar were partially added,
Mix until uniform. After the remaining powdered sugar is added and further mixed and kneaded, 0.5% by weight of the mugwort extract obtained in Example 1, an appropriate amount of a flavor and a coloring agent are added, and finish mixing is performed.
After that, it is extruded with an extruder, passed through several stages of rolling rollers, and then cooled to 5 to 15 ° C. It was cut into a prescribed size with a cutting roll, left for one day at 20 to 25 ° C., and then chewing gum was obtained.

The cosmetics, detergent compositions, oral compositions, and foods obtained in Examples 4 to 12 have no contact sensitizing potential, and are effective for wound healing, prevention of rough skin, improvement of rough skin, and It was also effective in preventing itching, body odor and bad breath.

[0053]

EFFECTS OF THE INVENTION The mugwort extract of the present invention has good stability because the cause of browning and precipitation has been removed, and also has high safety because it has also removed sensitizing substance. Therefore, various compositions containing the mugwort extract, that is, cosmetics, detergent compositions, oral compositions, foods, etc., have less coloring on clothes, and further wound healing, prevention of rough skin, improvement of rough skin, etc. It was also effective in preventing itching of skin, body odor and bad breath.

[Brief description of drawings]

FIG. 1 is a gel permeation chromatogram diagram of a protein having an average molecular weight of 15,000.

FIG. 2 is a gel permeation chromatogram diagram of the heat extract in Example 1 of the present invention.

FIG. 3 is a gel permeation chromatogram of the mugwort extract obtained in Example 2 of the present invention.

FIG. 4 is a gel permeation chromatogram diagram of a crude extract that has not been reextracted with 70% ethanol in Example 2 of the present invention.

FIG. 5 is an infrared absorption spectrum diagram of a mugwort extract of the present invention and an enzyme protein (protease) according to the potassium bromide tablet method.

Claims (5)

[Claims] 1. A mugwort extract in which a protein having an average molecular weight of 15,000 is substantially removed. 2. A cosmetic comprising the mugwort extract according to claim 1. 3. A detergent composition comprising the mugwort extract according to claim 1. 4. An oral composition comprising the mugwort extract according to claim 1. 5. A food containing the mugwort extract according to claim 1.