JPH06205673A - 内因性因子−西洋ワサビ大根ペルオキシダーゼ結合体 - Google Patents

内因性因子−西洋ワサビ大根ペルオキシダーゼ結合体

Info

Publication number: JPH06205673A
Authority: JP; Japan
Prior art keywords: conjugate; horseradish; peroxidase; radish peroxidase; endogenous factor
Prior art date: 1992-09-04
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Pending

Application number

JP22126793A

Other languages

English (en)

Japanese (ja)

Inventor

Dean William Schroer

ディーン・ウィリアム・シュローアー

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Becton Dickinson and Co

Original Assignee

Becton Dickinson and Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1992-09-04

Filing date

1993-09-06

Publication date

1994-07-26

1993-09-06 Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co

1994-07-26 Publication of JPH06205673A publication Critical patent/JPH06205673A/ja

Status Pending legal-status Critical Current

Links

108010001336 Horseradish Peroxidase Proteins 0.000 title claims 6
102000003992 Peroxidases Human genes 0.000 claims abstract description 20
108040007629 peroxidase activity proteins Proteins 0.000 claims abstract description 20
230000001590 oxidative effect Effects 0.000 claims abstract description 12
150000001720 carbohydrates Chemical class 0.000 claims abstract description 6
238000012875 competitive assay Methods 0.000 claims abstract description 5
229930003779 Vitamin B12 Natural products 0.000 claims abstract description 4
239000011715 vitamin B12 Substances 0.000 claims abstract description 4
235000019163 vitamin B12 Nutrition 0.000 claims abstract description 4
FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 claims abstract 3
240000003291 Armoracia rusticana Species 0.000 claims description 18
235000011330 Armoracia rusticana Nutrition 0.000 claims description 18
241000220259 Raphanus Species 0.000 claims description 18
235000006140 Raphanus sativus var sativus Nutrition 0.000 claims description 17
238000006243 chemical reaction Methods 0.000 claims description 10
238000000034 method Methods 0.000 claims description 9
241000872931 Myoporum sandwicense Species 0.000 claims description 8
150000004681 metal hydrides Chemical class 0.000 claims description 6
235000014633 carbohydrates Nutrition 0.000 claims description 5
229910052987 metal hydride Inorganic materials 0.000 claims description 3
229910052755 nonmetal Inorganic materials 0.000 claims description 3
239000000700 radioactive tracer Substances 0.000 claims description 3
238000004519 manufacturing process Methods 0.000 claims 1
229940029329 intrinsic factor Drugs 0.000 abstract description 22
239000004215 Carbon black (E152) Substances 0.000 abstract 1
229930195733 hydrocarbon Natural products 0.000 abstract 1
150000002430 hydrocarbons Chemical class 0.000 abstract 1
238000003556 assay Methods 0.000 description 19
LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 8
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
239000007864 aqueous solution Substances 0.000 description 5
239000000872 buffer Substances 0.000 description 5
239000000758 substrate Substances 0.000 description 4
238000001514 detection method Methods 0.000 description 3
230000000694 effects Effects 0.000 description 3
239000000203 mixture Substances 0.000 description 3
239000007790 solid phase Substances 0.000 description 3
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
102000014914 Carrier Proteins Human genes 0.000 description 2
CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
229930003270 Vitamin B Natural products 0.000 description 2
230000002411 adverse Effects 0.000 description 2
239000011230 binding agent Substances 0.000 description 2
108091008324 binding proteins Proteins 0.000 description 2
239000007795 chemical reaction product Substances 0.000 description 2
AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 description 2
238000009792 diffusion process Methods 0.000 description 2
239000007788 liquid Substances 0.000 description 2
KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 2
230000035945 sensitivity Effects 0.000 description 2
239000000243 solution Substances 0.000 description 2
238000003860 storage Methods 0.000 description 2
LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
238000000108 ultra-filtration Methods 0.000 description 2
235000019156 vitamin B Nutrition 0.000 description 2
239000011720 vitamin B Substances 0.000 description 2
GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 1
KQDJTBPASNJQFQ-UHFFFAOYSA-N 2-iodophenol Chemical compound OC1=CC=CC=C1I KQDJTBPASNJQFQ-UHFFFAOYSA-N 0.000 description 1
YRNWIFYIFSBPAU-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C1=CC=C(N(C)C)C=C1 YRNWIFYIFSBPAU-UHFFFAOYSA-N 0.000 description 1
IJJWOSAXNHWBPR-HUBLWGQQSA-N 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-n-(6-hydrazinyl-6-oxohexyl)pentanamide Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)NCCCCCC(=O)NN)SC[C@@H]21 IJJWOSAXNHWBPR-HUBLWGQQSA-N 0.000 description 1
102000002260 Alkaline Phosphatase Human genes 0.000 description 1
108020004774 Alkaline Phosphatase Proteins 0.000 description 1
BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
102000004190 Enzymes Human genes 0.000 description 1
108090000790 Enzymes Proteins 0.000 description 1
102000002464 Galactosidases Human genes 0.000 description 1
108010093031 Galactosidases Proteins 0.000 description 1
DZGWFCGJZKJUFP-UHFFFAOYSA-N Tyramine Natural products NCCC1=CC=C(O)C=C1 DZGWFCGJZKJUFP-UHFFFAOYSA-N 0.000 description 1
238000002835 absorbance Methods 0.000 description 1
230000002378 acidificating effect Effects 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
239000011248 coating agent Substances 0.000 description 1
238000000576 coating method Methods 0.000 description 1
238000004040 coloring Methods 0.000 description 1
230000002860 competitive effect Effects 0.000 description 1
238000002967 competitive immunoassay Methods 0.000 description 1
238000010168 coupling process Methods 0.000 description 1
230000001419 dependent effect Effects 0.000 description 1
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
239000012467 final product Substances 0.000 description 1
125000002485 formyl group Chemical class [H]C(*)=O 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
238000010166 immunofluorescence Methods 0.000 description 1
238000011065 in-situ storage Methods 0.000 description 1
230000000155 isotopic effect Effects 0.000 description 1
239000007791 liquid phase Substances 0.000 description 1
239000012280 lithium aluminium hydride Substances 0.000 description 1
-1 lithium aluminum hydride Chemical compound 0.000 description 1
HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 1
239000011325 microbead Substances 0.000 description 1
238000012986 modification Methods 0.000 description 1
230000004048 modification Effects 0.000 description 1
238000012544 monitoring process Methods 0.000 description 1
230000007935 neutral effect Effects 0.000 description 1
239000002953 phosphate buffered saline Substances 0.000 description 1
229910000160 potassium phosphate Inorganic materials 0.000 description 1
235000011009 potassium phosphates Nutrition 0.000 description 1
230000003389 potentiating effect Effects 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
239000012266 salt solution Substances 0.000 description 1
210000002966 serum Anatomy 0.000 description 1
239000007974 sodium acetate buffer Substances 0.000 description 1
229910000033 sodium borohydride Inorganic materials 0.000 description 1
239000012279 sodium borohydride Substances 0.000 description 1
229910000029 sodium carbonate Inorganic materials 0.000 description 1
239000000126 substance Substances 0.000 description 1
238000012360 testing method Methods 0.000 description 1
229960003732 tyramine Drugs 0.000 description 1
DZGWFCGJZKJUFP-UHFFFAOYSA-O tyraminium Chemical compound [NH3+]CCC1=CC=C(O)C=C1 DZGWFCGJZKJUFP-UHFFFAOYSA-O 0.000 description 1
238000012795 verification Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/82—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving vitamins or their receptors

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Genetics & Genomics (AREA)
Molecular Biology (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Medicinal Chemistry (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Immunology (AREA)
Urology & Nephrology (AREA)
Hematology (AREA)
General Engineering & Computer Science (AREA)
Analytical Chemistry (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Physics & Mathematics (AREA)
Food Science & Technology (AREA)
Cell Biology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)

JP22126793A 1992-09-04 1993-09-06 内因性因子−西洋ワサビ大根ペルオキシダーゼ結合体 Pending JPH06205673A (ja)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US94059992A	1992-09-04	1992-09-04
US940599		1992-09-04

Publications (1)

Publication Number	Publication Date
JPH06205673A true JPH06205673A (ja)	1994-07-26

Family

ID=25475128

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP22126793A Pending JPH06205673A (ja)	1992-09-04	1993-09-06	内因性因子−西洋ワサビ大根ペルオキシダーゼ結合体

Country Status (6)

Country	Link
JP (1)	JPH06205673A (fr)
AU (1)	AU662235B2 (fr)
CA (1)	CA2104413C (fr)
DE (1)	DE4329530A1 (fr)
FR (1)	FR2695406B1 (fr)
GB (1)	GB2270315A (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US7790363B2 (en)	2005-02-07	2010-09-07	Abbott Laboratories Inc.	Diagnostic test for vitamin B12
US8288124B2 (en)	2008-11-20	2012-10-16	Abbott Laboratories	Cloning, expression and purification of recombinant porcine intrinsic factor for use in diagnostic assay

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
AU660837B2 (en) *	1992-09-04	1995-07-06	Becton Dickinson & Company	Indirect chromatographic antigen sandwich test for detection of specific antibody and device therefor
US5350674A (en) *	1992-09-04	1994-09-27	Becton, Dickinson And Company	Intrinsic factor - horse peroxidase conjugates and a method for increasing the stability thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS6178385A (ja) *	1984-09-25	1986-04-21	Toyobo Co Ltd	安定なペルオキシダ−ゼ組成物
JPS62126977A (ja) *	1985-11-21	1987-06-09	ベ−リンガ−・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング	水溶性の安定化されたペルオキシダ−ゼ誘導体、及びその製法

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US4256833A (en) *	1979-01-08	1981-03-17	Majid Ali	Enzyme immunoassay for allergic disorders
FR2502786B1 (fr) *	1981-03-24	1985-06-21	Stallergenes Laboratoire	Procede de fixation d'antigenes et d'anticorps sur support de polysaccharides, et utilisation du produit ainsi obtenu pour les dosages immunologiques
DE3525911A1 (de) *	1985-07-19	1987-01-29	Boehringer Mannheim Gmbh	Konjugat fuer die enzymimmunobestimmung
WO1989012826A1 (fr) *	1988-06-15	1989-12-28	Beckman Instruments, Inc.	Titrage de la vitamine b12
US5264304A (en) *	1991-09-23	1993-11-23	W. R. Grace & Co.-Conn.	Battery separators with T-shaped ribs
AU659754B2 (en) *	1992-09-04	1995-05-25	Becton Dickinson & Company	Chromatographic antigen sandwich test for detection of specific antibody and device therefor
AU660837B2 (en) *	1992-09-04	1995-07-06	Becton Dickinson & Company	Indirect chromatographic antigen sandwich test for detection of specific antibody and device therefor

1993
- 1993-08-19 CA CA 2104413 patent/CA2104413C/fr not_active Expired - Fee Related
- 1993-08-19 AU AU44764/93A patent/AU662235B2/en not_active Ceased
- 1993-08-20 GB GB9317356A patent/GB2270315A/en not_active Withdrawn
- 1993-09-02 DE DE19934329530 patent/DE4329530A1/de not_active Withdrawn
- 1993-09-03 FR FR9310526A patent/FR2695406B1/fr not_active Expired - Fee Related
- 1993-09-06 JP JP22126793A patent/JPH06205673A/ja active Pending

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS6178385A (ja) *	1984-09-25	1986-04-21	Toyobo Co Ltd	安定なペルオキシダ−ゼ組成物
JPS62126977A (ja) *	1985-11-21	1987-06-09	ベ−リンガ−・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング	水溶性の安定化されたペルオキシダ−ゼ誘導体、及びその製法

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US7790363B2 (en)	2005-02-07	2010-09-07	Abbott Laboratories Inc.	Diagnostic test for vitamin B12
US7932369B2 (en)	2005-02-07	2011-04-26	Abbott Lab	Diagnostic test for vitamin B12
US8288124B2 (en)	2008-11-20	2012-10-16	Abbott Laboratories	Cloning, expression and purification of recombinant porcine intrinsic factor for use in diagnostic assay

Also Published As

Publication number	Publication date
AU662235B2 (en)	1995-08-24
FR2695406B1 (fr)	1995-09-29
FR2695406A1 (fr)	1994-03-11
GB9317356D0 (en)	1993-10-06
CA2104413C (fr)	1996-05-21
GB2270315A (en)	1994-03-09
AU4476493A (en)	1994-03-10
CA2104413A1 (fr)	1994-03-05
DE4329530A1 (de)	1994-03-10

Publication	Publication Date	Title
JP2509795B2 (ja)	1996-06-26	内因性因子−西洋ワサビ大根ペルオキシダ―ゼ結合体およびその安定性を増大させる方法
JP3009155B2 (ja)	2000-02-14	免疫クロマトグラフイー分析方法
EP0093613B1 (fr)	1988-08-17	Procédé d'étalonnage simultané pour une méthode d'analyse immunologique hétérogène, dispositif et trousse de réactifs diagnostiques
Morris et al.	1981	Flavin adenine dinucleotide as a label in homogeneous colorimetric immunoassays
US4686181A (en)	1987-08-11	Specific binding assay employing anti-G6PDH as label
CA1261743A (fr)	1989-09-26	Produit pour les essais de diagnostic biologiques et procede utilisant des fragments fab marques
Kohen et al.	1980	Antibody-enhanced hydrolysis of steroid esters
JP3103379B2 (ja)	2000-10-30	レセプター：遊離リガンド（リランド）複合体ならびにそれに基づくアッセイおよびキット
US4622293A (en)	1986-11-11	Iodothyronine immunoassays employing HMS as TBP blocking agent
AU703944B2 (en)	1999-04-01	Ultrasensitive process for assaying cardiac troponin I
JPH06205673A (ja)	1994-07-26	内因性因子−西洋ワサビ大根ペルオキシダーゼ結合体
JPH0646893A (ja)	1994-02-22	分析物の測定方法および測定手段
JPH066074B2 (ja)	1994-01-26	ルミノ−ルまたは７−ジアルキルアミノナフタリン−１，２−ジカルボン酸−ヒドラジドの酸化の際の量子収率を高める方法及びペルオキシダ−ゼ測定用試薬
JPS62165151A (ja)	1987-07-21	標識ヒダントイン接合体並びにその分析要素及び免疫分析での使用
JPS61186855A (ja)	1986-08-20	チロキシンを形成するグロブリンの結合能力を測定する方法
JPS5886461A (ja)	1983-05-24	免疫試験用試薬、試験キツト及びそれらを用いる免疫試験法
JPH0587809A (ja)	1993-04-06	特定タンパク質の糖化割合の測定方法
Doná	1985	Homogeneous colorimetric enzyme inhibition immunoassay for cortisol in human serum with Fab anti-glucose 6-phosphate dehydrogenase as a label modulator
WO1990011526A1 (fr)	1990-10-04	Diagnostic de cancer
JPH11237382A (ja)	1999-08-31	競合的アポペルオキシダーゼ検定法
JP2980681B2 (ja)	1999-11-22	クマリン誘導体を用いた物質の定量方法
JP3167176B2 (ja)	2001-05-21	蛍光酵素免疫測定法
JPS62180269A (ja)	1987-08-07	遊離型サイロキシンの酵素免疫測定法
Ngo	1985	Prosthetic group labeled enzyme immunoassay
Capaldi	1983	STUDIES ON HUMAN ERYTHROCYTE MEMBRANE SURFACE CARBOHYDRATES.