JPH07304648A - Skin-beautifying cosmetic - Google Patents

Abstract

PURPOSE:To provide a skin-beautifying cosmetic capable of depressing the tyrosinase activity of cultured melanocyte, suppressing melanogenesis and pigmentation caused by ultraviolet irradiation and improving dark skin by compounding an extract produced by extracting cereals, etc., with a neutral medium. CONSTITUTION:This cosmetic is produced by compounding an extract obtained by extracting cereal, or refining residue of cereal, preferably rice bran or wheat bran with a neutral medium. The extract to be compounded is preferably decomposed by treating with a combination of (A) an actinase and (B) at least one kind of proteinases selected from pepsins, trypsins, papains, peptidases and bromelain. The extraction solvent is preferably refined water or a mixture of refined water and ethanol, glycerol or 1,3-butylene glycol and the pH of the solvent is preferably adjusted with NaOH, etc.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a whitening cosmetic composition.
More specifically, the present invention relates to a whitening cosmetic composition which has an excellent whitening effect and can be suitably used for basic cosmetics, makeup cosmetics, bath agents and the like.

[0002]

2. Description of the Related Art Conventionally, various studies have been carried out and various whitening cosmetics have been proposed as the need for cosmetics having a whitening effect increases. However, none of the conventional whitening cosmetics fully satisfies the whitening effect in addition to the safety and storage stability for the skin and the like.

[0003]

Therefore, as a result of intensive studies conducted by the present inventors in view of the above-mentioned prior art, an extract obtained by extracting a grain or a residue generated during grain refinement with a neutral medium. Found to have excellent effects such as reducing the thyrodinase activity of cultured pigment cells, suppressing the production of melanin, suppressing pigmentation caused by ultraviolet irradiation, and improving dullness, and thus completing the present invention. Came to

[0004]

That is, the present invention relates to a whitening cosmetic composition containing an extract obtained by extracting a grain or a refined residue of a grain with a neutral medium.

[0005]

Actions and Examples As described above, the whitening cosmetic composition of the present invention contains the extract obtained by extracting the grain or the refined residue of the grain with a neutral medium.

Examples of grains used in the present invention include rice and wheat. Further, as the refined residue of grain, for example, rice bran, bran, corn refined residue,
Soybean refined residue and the like can be mentioned, but among these,
Rice bran and bran are preferred because the extract obtained by extracting with a neutral medium described below has a superior whitening effect.

Solvents used when extracting the grain or the grain refinement residue with a neutral medium include, for example, water such as purified water; monovalent lower alcohols such as ethanol; oleyl alcohol, stearyl alcohol, octyldodeca. Monohydric higher alcohols such as norls; polyols such as ethylene glycol, propylene glycol, glycerin, and 1,3-butylene glycol; ketones such as acetone; esters such as ethyl acetate; carbonization of hexane, chloroform, benzene, etc. Examples of the solvent include hydrogen solvents, which may be used alone or in combination of two or more. Among these, purified water and a mixed solvent of purified water and one or more of ethanol, glycerin and 1,3-butylene glycol are preferable since they can be widely applied to cosmetics. .

When the above mixed solvent is used,
For example, in the case of a mixed solvent of purified water and ethanol, the volume ratio of both is 1: 1 to 25: 1, and in the case of a mixed solvent of purified water and glycerin, the volume ratio of both is 1: 1. ~
In the case of a mixed solvent of 15: 1 and purified water and 1,3-butylene glycol, the volume ratio of both is preferably 1: 1 to 15: 1.

In the present invention, when the grain or the refined residue of the grain is extracted with a neutral medium, the pH of the extraction solution containing the grain or the refined residue of the grain may be about 5 to 9,
The solvent may be used as it is, for example, sodium hydroxide, sodium salts such as sodium carbonate, an alkalinity adjusting agent such as potassium salts such as potassium hydroxide,
For example, an acid adjusting agent such as citric acid, hydrochloric acid, phosphoric acid or sulfuric acid may be added to the solvent to adjust the pH to a desired value. Among these regulators, sodium hydroxide, sodium carbonate,
Hydrochloric acid and phosphoric acid are preferred.

The time required for the extraction treatment cannot be unconditionally determined because it depends on the type of solvent used, the target pH, the extraction temperature, etc.
In the case of 5 to 9, it is preferable that the temperature is usually 6 hours to 7 days at room temperature, especially 12 to 48 hours. In addition,
The extraction temperature is preferably about 4 to 40 ° C, more preferably about 10 to 30 ° C.

The thus-obtained extract may be blended as it is into a whitening cosmetic composition, for example, concentrated under reduced pressure to adjust the concentration, and then blended, for example, a freeze-drying method or a spray-drying method. You may mix | blend what was pulverized by.

The blending amount of the extract cannot be unconditionally determined because it varies depending on the kind of the whitening cosmetic intended, but if the blending amount is too small, the extract is blended. Since the whitening effect may not be fully manifested due to the above, it should be adjusted to 0.0005 parts or more, especially 0.005 parts or more in terms of solid content for 100 parts (weight part, the same below) of whitening cosmetics. When it is too much, it tends to be technically difficult to stably mix the extract with a whitening cosmetic, so that it is 5 in terms of solid content per 100 parts of whitening cosmetic. It is preferable to adjust the number of parts to be 1 part or less, especially 1 part or less.

Furthermore, in the present invention, the above-mentioned extract is treated with an enzyme because the whitening effect such as the reduction of thyrodinase activity, the inhibition of melanin production and the inhibition of pigmentation caused by ultraviolet irradiation is greater. It is preferable to blend the obtained decomposition product.

Examples of the enzyme include actinases such as actinase, pepsins such as pepsin, trypsin such as trypsin and chymotrypsin, papains such as papain and chymopapain, glycylglycine peptidase, carboxypeptidase, aminopeptidase. And peptidases such as and proteolytic enzymes such as bromelain. Among them, a combination of actinase and at least one proteolytic enzyme selected from pepsins, trypsins, papains, peptidases and bromelain is a whitening makeup containing the obtained degradation product. The combination of actinase with pepsin and trypsin is particularly preferable because the material is excellent in storage stability and safety.

In the present invention, when treating with two or more kinds of enzymes, usually one kind of enzyme is used at one time.

The amount of enzyme used per one time when performing the enzyme treatment is about 0.0005 to 0.05 parts, especially 0.001 to 0.005 parts, relative to 100 parts of the neutral extraction solution containing the grain and the refined residue of the grain. It is about 0.003 to 0.01 in total
About 5 parts is preferable from the viewpoint of the action and effect of such an enzyme.

The time required for the enzyme treatment cannot be unconditionally determined because it depends on the type of the enzyme used and the decomposition temperature, but it is usually about 30 minutes to 24 hours per enzyme, especially 1 to 4 hours. It is preferably about the same. The decomposition temperature of the above-exemplified enzyme is about 30-
It is 50 ° C.

Further, when the enzyme treatment is carried out, the pH of the extraction solution may be adjusted to the optimum pH of the enzyme to be used. To adjust the pH of the extraction solution, for example, if necessary, for example, An acidic modifier, an alkaline modifier, or the like used in the extraction can be used.

The decomposed product thus obtained may be blended into the whitening cosmetic composition as it is, or may be blended after concentration under reduced pressure to adjust the concentration, for example, by a freeze-drying method or a spray-drying method. You may mix powdered thing.

The solution containing the obtained decomposition product is preferably adjusted to pH 4 to 8 from the viewpoint of safety to the skin.

The amount of the decomposition product to be blended cannot be determined unconditionally because it depends on the type of the whitening cosmetic product to be intended, but if the amount is too small, the decomposition product is blended. As a result, the whitening effect tends to not be fully manifested, so 0.0005 parts or more in solid content per 100 parts of whitening cosmetic, especially 0.005 parts
It is preferable to adjust the amount to be more than 1 part, and when it is too much, it tends to be technically difficult to blend the decomposed product into the whitening cosmetic composition, so the whitening cosmetic composition 100 It is preferable that the amount is adjusted to 5 parts or less, especially 1 part or less, in terms of solid content, relative to the parts.

The extract and the degradation product used in the present invention simultaneously exhibit the effects of lowering the thyrodinase activity of cultured pigment cells, suppressing melanin production and suppressing pigmentation caused by UV irradiation. When the whitening cosmetic composition of the present invention containing a decomposed product is used, the expression of spots, freckles, etc. due to the accumulation of melanin is suppressed, and white and beautiful skin with an improved dull feeling is maintained.

The whitening cosmetic composition of the present invention, as described above,
An extract obtained by extracting a grain or a refined residue of a grain with a neutral medium, or a decomposed product obtained by treating the extract with an enzyme is blended. In addition to, for example, excipients and fragrances generally used in cosmetics, oils and fats, surfactants, moisturizers,
Various cosmetic ingredients such as a whitening agent, a pH adjusting agent, a thickening agent, a preservative, an antioxidant, an ultraviolet absorber, a pigment, a detergent, a desiccant, and an emulsifier can be appropriately added to the whitening cosmetic composition.

As the oils and fats, vegetable oils and fats commonly used in cosmetics, such as liquid paraffin, paraffin, cetanol, avocado oil, olive oil, jojoba oil and coconut oil; beef tallow, lard, horse fat, Animal fats and oils such as turtle oil, mink oil, perserine oil, squalane;
Examples thereof include synthetic oils and fats such as methylpolysiloxane, behenyl alcohol, glyceryl tricaprylic caprate, glyceryl trioctanoate, glyceryl triisopalmitate, and silicone oil.

Examples of the above-mentioned surfactant include anionic surfactants such as sodium lauryl sulfate, triethanolamine lauryl sulfate and diethanolamide lauric acid; stearyltrimethylammonium chloride, cetyltrimethylammonium chloride, benzalkonium chloride and the like. Cationic surfactants; nonionic surfactants such as glyceryl monostearate, sorbitan monostearate, polyoxyethylene (20) sorbitan monostearate, polyoxyethylene hydrogenated castor oil, sucrose ester, fatty acid amide, etc. Can be given.

Examples of the moisturizer include glycerin, propylene glycol, 1,3-butylene glycol,
Synthetic humectants such as sodium pyrrolidone carboxylic acid and pantothetein-S-sulfonate; hyaluronic acid, collagen, elastin, placenta extract, royal jelly, microbial fermentation liquid such as chitin, chitosan, pectin and the like,
Other examples include natural moisturizing liquids such as extracts derived from plants and animals.

Examples of the whitening agent include kojic acid,
In addition to ascorbic acid, arbutin, placenta extract and derivatives thereof, other plant- and animal-derived extracts and the like can be mentioned.

Examples of the pH adjusting agent include organic acids such as citric acid and sodium citrate, and salts thereof.

Examples of the thickener include carboxymethyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, carboxyvinyl polymer,
Examples thereof include polyvinyl alcohol, tragacanth gum, sodium alginate, carrageenan and the like.

Examples of the preservatives include paraoxybenzoic acid esters such as methylparaben, ethylparaben, propylparaben and butylparaben, phenoxyethanol, ethanol and dehydroacetic acid.

Examples of the antioxidant include vitamin E, butyloxytoluene (BHT), butyloxyanisole (BHA) and the like.

Examples of the pigment include red iron oxide, yellow iron oxide, black iron oxide, titanium oxide, nylon powder, sericite, mica, talc and the like.

Examples of the cleaning agent include sodium lauryl sulfate.

Examples of the emulsifier include soybean lecithin oil and the like.

Examples of the excipient include sodium sulfate and the like.

The blending amount of each of these cosmetic ingredients cannot be unconditionally determined because it varies depending on the intended use of the whitening cosmetic, and it is preferably adjusted appropriately according to the intended use.

The form of the whitening cosmetic composition of the present invention is arbitrary,
Although not particularly limited, the whitening cosmetic composition of the present invention prevents the appearance of dullness, blemishes and freckles on the skin, maintains youthful and healthy skin, and maintains a white and beautiful skin condition. Because of its excellent properties, for example, basic cosmetics such as creams, emulsions, lotions, essences, facial cleansers, packs, lipsticks, foundations, liquid foundations, makeup cosmetics such as press powders, body soaps, toiletries such as soaps, etc. Can be used as

Furthermore, when the above extract or decomposed product and the dry powder thereof are added to hot water, it is effective for improving the condition of the skin by percutaneous absorption. Therefore, the whitening cosmetic composition of the present invention is It can also be used as a bath agent. Thus, when the whitening cosmetic of the present invention is used as a bath agent, the amount of the extract or the decomposed product to be added to the whitening cosmetic has an effect of improving the skin condition exhibited by the extract or the decomposed product. Considering this, 0.0005 to 5.0 parts by solid content of the extract or decomposed product for 100 parts of whitening cosmetic, especially
It is preferably 0.001 to 0.1 part. When the bath agent is used, the amount of the bath agent used is preferably adjusted so that the bath agent is usually used in an amount of about 5 to 50 g per 200 liters of hot water.

Next, the whitening cosmetic composition of the present invention will be described in more detail based on examples, but the present invention is not limited to these examples.

Preparation Example 1 (Production of neutral rice extract) 200 g of rice was added to 800 ml of purified water, the pH was adjusted to 6.0 to 8.0 with 0.1N aqueous sodium hydroxide solution, and the pH was adjusted to about 24 at room temperature.
It was immersed for a period of time for extraction to obtain about 480 ml of extract (solid content: about 1.5% by weight).

Preparation Example 2 (Production of Enzymatic Decomposition Product of Neutral Rice Extract) The extract obtained in Preparation Example 1 was treated with actinase (optimum pH).
8.0) Add 5 mg at 30-40 ° C for 1-2 hours, then add 5 mg of pepsin (optimum pH 2.0) at 30-40 ° C for 1-2 hours. Finally, 5 mg of trypsin (optimum pH 8.0) was added and the treatment was carried out at 30 to 40 ° C. for 1 to 2 hours.

When each enzyme was added, the extract was adjusted to have the optimum pH for each enzyme.

This was filtered to obtain about 250 ml of a pale yellow and transparent enzyme decomposition product solution (solid content: about 2.0% by weight).

Preparation Example 3 (Production of Rice Bran Neutral Extract) Preparation Example 1 was repeated in the same manner as in Preparation Example 1 except that rice bran was used instead of rice, and the extract (solid content: about 1.5% by weight) was added. I got 480 ml.

Preparation Example 4 (Production of Enzymatic Degradation Product of Neutral Rice Bran Extract) In Preparation Example 2, except that the extract solution obtained in Preparation Example 3 was used in place of the extract solution obtained in Preparation Example 1. A pale yellow transparent enzyme-decomposed product solution (solid content:
About 2.0% by weight) About 250 ml was obtained.

Preparation Example 5 (Production of Enzymatic Degradation Product of Neutral Extract of Rice Bran) Pale yellow in the same manner as in Preparation Example 4 except that papain (optimum pH 7.0) was used in place of pepsin. Clear enzyme digest solution (solid content: about 2.0% by weight) About
I got 250 ml.

Preparation Example 6 (Production of Enzymatic Degradation Product of Neutral Bran Extract) 200 g of bran was added to 800 ml of purified water, and the mixture was added with 0.1N hydrochloric acid to p.
The H content was adjusted to 6.0 to 8.0, and the mixture was immersed at room temperature for about 24 hours for extraction to obtain about 480 ml of the extract.

Treatment was carried out in the same manner as in Preparation Example 4, except that actinase, pepsin and trypsin (10 mg each) were sequentially added to the obtained extract.

This was filtered to obtain about 300 ml of a pale yellow transparent enzyme-decomposed product solution (solid content: about 2.0% by weight).

Preparation Example 7 (Production of freeze-dried product of neutralized rice bran extract) 100 g of the extract obtained in Preparation Example 3 was concentrated and then freeze-dried under vacuum to obtain about 1.5 g of a dry powder of the extract. I got it.

Preparation Example 8 (Production of a spray-dried product of an enzymatic decomposition product of a rice bran neutral extract) 250 g of the enzymatic decomposition product solution obtained in Preparation Example 4 was spray-dried to give a dry powder of about 5 g. I got it.

Next, the following tests were carried out using the extract obtained in Preparation Example 3 and the enzyme-decomposed product solution obtained in Preparation Example 4.

Test Example 1 (Inhibition of intracellular tyrosinase activity) Cultured B16 mouse melanoma cells were seeded at 8000 cells / well in a 96-well microplate (CORNING) and 3% by volume.
Calf serum containing Eagle's minimum essential medium (MEM) at 37 ℃,
After pre-culturing for 24 hours under the condition of 5% CO ₂ , 3% by volume calf to which 5% by volume or 10% by volume of the extract obtained in Preparation Example 3 or the enzyme decomposition product solution obtained in Preparation Example 4 was added. Replace with serum-containing Eagle MEM, and further at 37 ℃, 5%
It was cultured for 48 hours under the condition of CO ₂ .

Then, after removing the medium and washing with PBS (-), 0.1N phosphate buffer and 5 mM L-DOPA were added and incubated at 37 ° C for 1 hour to determine the amount of dopachrome produced in the microplate. Leader (BIO
RAD).

FIG. 1 (Preparation Example 3) shows the relationship between the addition amount of the extract or the enzyme decomposition product solution and the production amount of dopachrome.
The results are shown in the graphs of the case of using the extract obtained in (4) and the case of using the solution of the enzymatic decomposition product obtained in Preparation Example 4). The graphs of FIGS. 1 and 3 show the amount of dopachrome produced when no extract or enzyme-decomposed product solution was added at 100%.

Further, the reduced nicotinamide adenisine dinucleotide (hereinafter referred to as N) in mitochondria by the MTT reduction method showing the amount of the extract or the amount of the enzyme-decomposed solution added and the cell activity of the cultured B16 mouse melanoma cells.
A graph showing the relationship with the amount of ADH in FIG. 2 (when the extract obtained in Preparation Example 3 is used) and FIG. 4 (when the enzyme hydrolyzate solution obtained in Preparation Example 4 is used). Shown in.
Note that the graphs of FIGS. 2 and 4 show the amount of NADH in mitochondria by the MTT reduction method as 100% when no extract or enzyme-decomposed product solution was added.

As is clear from the graphs shown in FIGS. 1 and 2, the extract obtained in Preparation Example 3 almost inhibits the cell activity of cultured B16 mouse melanoma cells as the amount of the extract added increases. It can be seen that the amount of dopachrome produced is decreased without any effect and the intracellular tyrosinase activity is suppressed.

Further, as is clear from the graphs shown in FIGS. 3 and 4, the enzyme-decomposed product solution obtained in Preparation Example 4 increased the cell activity of cultured B16 mouse melanoma cells as the amount of addition increased. It can be seen that the amount of dopachrome produced is remarkably reduced and the intracellular tyrosinase activity is remarkably suppressed without inhibiting the above.

Test Example 2 (Melanin Production Inhibitory Action) Cultured B16 mouse melanoma cells were seeded in a petri dish (CORNING) having an inner diameter of 60 mm in an amount of 10,000, and the conditions were 37 ° C. and 5% CO _{2 using} an Eagle MEM containing 5% by volume of calf serum. After preculturing for 2 days under the following conditions, the enzyme digest solution obtained in Preparation Example 4 was replaced with an Eagle MEM containing 3% by volume of fetal calf serum containing 5% by volume or 10% by volume, and further at 37 ° C. and 5% C.
The cells were cultured under O ₂ for 3 days.

Then, after removing the medium and washing with PBS (-), the cells were detached with trypsin, the number of cells was counted, and at the same time, high temperature heat treatment was performed with a 1N sodium hydroxide aqueous solution containing 10% dimethyl sulfoxide, Variable spectrophotometer (manufactured by Hitachi, Ltd.,
U-2000) was used to measure the amount of melanin produced.

The graph of FIG. 5 shows the relationship between the added amount of the enzymatic degradation product solution and the produced amount of melanin per 10 ⁶ cells.
The graph of FIG. 5 shows the amount of melanin produced when the enzyme-decomposed product solution was not added at 100%.

As is clear from the graph shown in FIG. 5, the enzyme-decomposed product solution obtained in Preparation Example 4 markedly suppressed the production of melanin in the cultured B16 mouse melanoma cells as the amount of addition increased. I understand.

Test Example 3 (Safety to Skin) Using the enzyme-decomposed product solutions obtained in Preparation Examples 2 and 4, the safety to human skin was examined by conducting a closed patch test.

20 randomly selected healthy adult men and women aged 20 to 70 years were used as subjects, and 0.2 ml of the enzyme hydrolyzate solution obtained in Preparation Example 2 or Preparation Example 4 was placed on the skin of the upper arm of each subject. Each was applied, and a human patch test adhesive plaster (manufactured by River Tape Co., Ltd.) was applied from above.

As a control, the human patch test adhesive bandage was applied so that the circular cloth portions thereof were in parallel with the portions to which the enzyme decomposition product solution was applied.

After 48 hours, each human patch test adhesive bandage was removed, and the skin symptoms of the site to which the enzymatic degradation product solution was applied and the control site were visually observed, and the closed patch test was evaluated by the Japan Patch Test Study Group. It evaluated based on the standard.

As a result, no temporary irritation to the human skin of the enzyme-decomposed product solutions obtained in Preparation Examples 2 and 4 was observed, and all of these enzyme-decomposed product solutions had excellent skin safety. I understand.

Formulation Examples 1 to 12 and Comparative Formulation Examples 1 to 3 Formulation Example 1 (Cream) [(A) component] (part) Liquid paraffin 5.0 Hexalan (glyceryl trioctanoate, manufactured by Technoble Co., Ltd.) 4.0 Paraffin 5.0 Cetanol 2.0 Glyceryl monostearate 2.0 Polyoxyethylene (20) sorbitan monostearate 6.0 Butylparaben 0.1 [(B) component] Extract solution obtained in Preparation Example 10.0 Glycerin 5.0 Carboxymethylcellulose sodium 0.1 Methylparaben 0.1 Moiston C (natural moisture) Rising Factor, manufactured by Technoble Co., Ltd. 1.0 Amount that makes the total amount of purified water 100.0 parts [(C) component] Fragrance 0.3 After heating the (A) component and (B) component above 80 ° C. respectively, (A) The component) and the component (B) were mixed and stirred. 50 this
After cooling to ℃, the component (C) was added and further mixed with stirring to prepare a uniform cream.

Formulation Example 2 (Cream) A cream was prepared in the same manner as in Formulation Example 1 except that the enzyme hydrolyzate solution obtained in Preparation Example 2 was used in place of the extract obtained in Preparation Example 1.

Formulation Example 3 (Cream) A cream was prepared in the same manner as in Formulation Example 1 except that the extract solution obtained in Preparation Example 3 was used instead of the extract solution obtained in Preparation Example 1.

Formulation Example 4 (Cream) A cream was prepared in the same manner as in Formulation Example 1 except that the enzyme hydrolyzate solution obtained in Preparation Example 4 was used in place of the extract obtained in Preparation Example 1.

Formulation Example 5 (milky lotion) [(A) component] (part) Liquid paraffin 6.00 Hexalan (glyceryl trioctanoate, manufactured by Technoble Co., Ltd.) 4.00 Jojoba oil 1.00 Polyoxyethylene (20) sorbitan monostearate 2.00 Soybean Lecithin oil 1.50 Methylparaben 0.15 Ethylparaben 0.03 [(B) component] Extract obtained in Preparation Example 1 30.00 Glycerin 3.00 1,3-Butylene glycol 2.00 Sodium carboxymethylcellulose 0.30 Sodium hyaluronate 0.01 Purified water Total amount to be 100.00 parts [Component (C)] Fragrance 0.05 The components (A) and (B) were heated to 80 ° C., respectively, and then the components (A) and (B) were mixed and stirred. After cooling this to 50 ° C., the component (C) was added and further stirred to prepare a uniform emulsion.

Formulation Example 6 (Lotion) [Components] (Parts) L-ascorbyl magnesium phosphate 2.0 Ethanol 10.0 Glycerin 3.0 1,3-Butylene glycol 2.0 Methylparaben 0.2 Citric acid 0.1 Sodium citrate 0.3 Carboxyvinyl polymer 0.1 Water-soluble placenta Extract 1.0: Extract obtained in Preparation Example 3 10.0 Fragment Trace amount of purified water 100.0 parts total amount The above components were mixed to prepare a uniform lotion.

Formulation Example 7 (Essence) [Components] (Parts) Ethanol 10.0 Glycerin 5.0 1,3-Butylene glycol 1.0 Oleyl alcohol 0.1 Polyoxyethylene sorbitan monolaurate ester (20EO) 1.0 Methylparaben 0.2 Citric acid 0.1 Sodium citrate 0.3 Carboxyvinyl polymer 0.3 Enzyme-decomposed product solution obtained in Preparation Example 1.0 Fragrance Trace amount of water-soluble collagen 1.0 Purified water Total amount of 100.0 parts The above components were mixed to prepare a uniform essence.

Formulation Example 8 (pack) [Components] (parts) Polyvinyl alcohol 15.0 Hydroxymethylcellulose 5.0 Propylene glycol 5.0 Ethanol 10.0 Methylparaben 0.1 Enzymatic degradation product solution obtained in Preparation Example 6 10.0 Perfume Trace purified water Total 100.0 parts The above components were mixed to prepare a uniform pack.

Formulation Example 9 (Facial cleanser) [Components] (Parts) Stearic acid 15.0 Lauric acid 5.0 Myristic acid 15.0 Glyceryl monostearate 4.0 Potassium hydroxide 7.0 Glycerin 8.0 Extract solution obtained in Preparation Example 10.0 Methylparaben 0.2 Purification Amount that makes the total amount of water 100.0 parts The above components were heated to 85 ° C and mixed to prepare a uniform face wash.

Formulation Example 10 (Bathing agent) [Components] (Parts) Sodium sulfate 35.0 Sodium hydrogen carbonate 52.0 Borax 2.0 Sodium carboxymethylcellulose 1.0 Red 201 Trace amount Fragrance Trace amount of freeze-dried product obtained in Preparation Example 7 Amount to be 100.0 parts The above ingredients were mixed to prepare a uniform bath preparation.

Formulation Example 11 (lipstick) [(A) component] (part) castor oil 50.0 octyldodecanol 5.0 lanolin 5.0 liquid lanolin 5.0 beeswax 4.0 ozokerite 7.0 candelilla wax 2.0 carnauba wax 1.0 [(B) component] titanium oxide 1.0 pigment (Red No. 201 etc.) Total 4.0 Spray-dried product obtained in Preparation Example 8 Total amount is 100.0 parts [(C) component] A small amount of perfume After heating the (A) component and (B) component, respectively. ,
The components (A) and (B) were mixed and stirred. This was reheated, the component (C) was added, and the mixture was poured into a mold and rapidly cooled to prepare a lipstick.

Formulation Example 12 (Liquid foundation) [(A) component] (part) Stearic acid 2.4 Propylene glycol monostearate 2.0 Cetostearyl alcohol 0.2 Liquid lanolin 2.0 Liquid paraffin 3.0 Isopropyl myristate 8.5 Propylparaben Suitable amount [(B) component ] The amount of the enzymatically decomposed product solution obtained in Preparation Example 4 is 100.0 parts. Sodium carboxymethylcellulose 0.2 Bentonite 0.5 Propylene glycol 4.0 Triethanolamine 1.1 Methylparaben Appropriate amount [(C) component] Titanium oxide 8.0 Talc 4.0 Coloring pigment Appropriate amount The above ( After heating component (A) and component (B) respectively,
The components (A) and (B) were mixed and stirred. This was reheated, the component (C) was added, and the mixture was poured into a mold and stirred until it reached room temperature to prepare a liquid foundation.

Comparative Prescription Example 1 (Cream) A cream was prepared in the same manner as in Prescription Example 1 except that purified water was used instead of the extract obtained in Preparation Example 1.

Comparative formulation example 2 (bath formulation) A bath formulation was prepared in the same manner as in formulation example 10 except that mannitol (D-mannitol) was used instead of the freeze-dried product obtained in preparation example 7.

Comparative Prescription Example 3 (Liquid Foundation) A liquid foundation was prepared in the same manner as in Prescription Example 12 except that purified water was used instead of the enzyme-decomposed product solution obtained in Preparation Example 4.

Example 1 Using the creams obtained in Formulation Example 1 and Comparative Formulation Example 1, the inhibitory effect on pigmentation caused by ultraviolet irradiation was examined.

Ten randomly selected healthy adult males aged 20 to 35 were used as test subjects, and the inner part of their forearm was 1 cm × 1 cm.
The two ultraviolet irradiation parts were set. Using a UV-B lamp (FL20-SE manufactured by Toshiba Corp.), an amount of ultraviolet rays corresponding to the minimum erythema dose (MED) of each subject measured in advance is once a day (morning) for 3 days. Irradiated continuously.

For 30 consecutive days from the start of UV irradiation, the UV irradiation period (first 3 days) was immediately after UV irradiation and twice a day in the evening, after the UV irradiation period (4th day or later).
Is a prescription example 1 for each UV irradiation part twice a day in the morning and in the evening.
About 0.01 g each of the cream obtained in Example 1 and the cream obtained in Comparative Prescription Example 1 was applied.

The state of pigmentation of the UV-irradiated part of each subject was visually observed every 5 days from the start of UV-irradiation, and the effect of suppressing the pigmentation when the cream obtained in Prescription Example 1 was applied was evaluated as follows. It evaluated based on the standard.

(Evaluation Criteria) A: At the place where the cream obtained in Formulation Example 1 was applied,
Almost no pigmentation is observed. B: At the place where the cream obtained in Formulation Example 1 was applied,
The pigmentation is apparently less than that in the area where the cream obtained in Comparative Prescription Example 1 was applied. C: At the place where the cream obtained in Formulation Example 1 was applied,
There is less pigmentation than the area where the cream obtained in Comparative Prescription Example 1 was applied. D: At the place where the cream obtained in Formulation Example 1 was applied,
Pigmentation is slightly less than that of the area where the cream obtained in Comparative Prescription Example 1 was applied.

The evaluation results of the pigmentation state every 5 days from the day on which the ultraviolet irradiation was started are expressed by the ratio of the number of people who performed each evaluation A to D when 10 subjects were 100%, and the occupancy ratio of each evaluation was shown. The change is shown in the graph of FIG.

Example 2 The same procedure as in Example 1 was carried out except that the cream obtained in Prescription Example 2 was used instead of the cream obtained in Prescription Example 1, and the dye in the ultraviolet irradiation part of each test subject was used. The state of deposition was evaluated.

The results of evaluation of the pigmentation state every 5 days from the start of ultraviolet irradiation are shown in the graph of FIG. 7 in the same manner as in Example 1.

In Examples 1 and 2, none of the subjects had skin abnormalities when the creams obtained in Formulation Examples 1 and 2 were applied.

The creams obtained in Formulation Examples 1 and 2 did not change in their condition within 30 days.

As is clear from the results shown in FIGS. 6 and 7, the cream obtained in Formulation Example 1 and Formulation Example 2 were obtained.
No matter which of the creams obtained in Example 1 was used, the effect of suppressing the pigmentation with the cream obtained in Comparative Prescription Example 1 was suppressed as the number of days passed from the start of ultraviolet irradiation. The difference is large, and it can be seen that the creams obtained in Formulation Examples 1 and 2 have an excellent pigmentation suppressing effect.

Further, comparing the graph of FIG. 6 with the graph of FIG. 7, when the cream obtained in Formulation Example 2 was used, the occupancy rate of A evaluation, which showed almost no pigmentation, was higher. From this, it can be seen that the enzyme-decomposed product solution obtained in Preparation Example 2 mixed with the cream of Formulation Example 2 exhibits a superior pigmentation-inhibiting action.

Example 3 The same procedure as in Example 1 was carried out except that the cream obtained in Prescription Example 3 was used instead of the cream obtained in Prescription Example 1, and the dye in the ultraviolet irradiation part of each subject was tested. The state of deposition was evaluated.

The results of evaluation of the pigmented state every 5 days from the start of ultraviolet irradiation are shown in the graph of FIG. 8 in the same manner as in Example 1.

Example 4 The same procedure as in Example 1 was repeated except that the cream obtained in Formulation Example 4 was used in place of the cream obtained in Formulation Example 1, and the dye in the ultraviolet irradiation part of each test subject was used. The state of deposition was evaluated.

The results of evaluation of the pigmented state every 5 days from the start of ultraviolet irradiation are shown in the graph of FIG. 9 in the same manner as in Example 1.

In Examples 3 and 4, none of the subjects had abnormal skin or the like when the creams obtained in Formulation Examples 3 and 4 were applied.

In addition, the creams obtained in Formulation Examples 3 and 4 did not change their state in 30 days.

As is clear from the results shown in FIGS. 8 and 9, the cream obtained in Formulation Example 3 and Formulation Example 4 were obtained.
No matter which of the creams obtained in Example 1 was used, the effect of suppressing the pigmentation with the cream obtained in Comparative Prescription Example 1 was suppressed as the number of days passed from the start of ultraviolet irradiation. The difference is large, and it can be seen that the creams obtained in Formulation Examples 3 and 4 have an excellent pigmentation suppressing effect.

Further, comparing the graph of FIG. 8 with the graph of FIG. 9, when the cream obtained in Formulation Example 4 was used, the occupancy rate of A evaluation in which almost no pigmentation was observed was extremely high. From this, it can be seen that the enzyme-decomposed product solution obtained in Preparation Example 4 which was blended with the cream of Formulation Example 4 exhibited an extremely excellent pigmentation-inhibiting action.

Example 5 Using the bath agents obtained in Formulation Example 10 and Comparative Formulation Example 2,
The inhibitory effect on skin pigmentation by bathing was examined.

Twenty healthy adult men and women aged 30 to 60 years randomly selected were used as test subjects, and 25 g of each bath agent was dissolved in 200 liters of hot water and bathed once a day for 1 month. After receiving, the state of pigmentation in the axilla of each subject group was visually observed and evaluated based on the following evaluation criteria. The results are shown in Table 1.

(Evaluation Criteria) A: Almost no pigmentation. B: Pigmentation was clearly reduced. C: There was less pigmentation than before using the bath agent. D: Almost no change from before using the bath agent. E: Pigmentation was increased rather than before using the bath agent.

[0106]

[Table 1]

As is clear from the results shown in Table 1,
When the bath preparation obtained in Formulation Example 10 was used, pigmentation was almost eliminated or reduced, so that the bath preparation obtained in Comparative Formulation Example 2 could hardly suppress pigmentation. On the other hand, it can be seen that the bath preparation obtained in Formulation Example 10 has an excellent pigmentation suppressing effect.

In Example 5, none of the subjects had abnormal skin or the like when taking a bath with the bath preparation obtained in Formulation Example 10.

In addition, the bath preparation obtained in Prescription Example 10 did not change its state within one month.

Example 6 Using the liquid foundations obtained in Prescription Example 12 and Comparative Prescription Example 3, the effect of improving dullness was examined.

Forty healthy adult women, 25-35 years old, who were randomly sampled were used as subjects, and the liquid foundation obtained in Formulation Example 12 was applied to the right cheek of each subject and Comparative Formulation Example 3 was applied to the left cheek of the subject. The amount of the liquid foundation obtained above is used once a day for 1 month (about 0.
After applying 1 g each, the left and right cheeks were visually observed and compared, and evaluated based on the following evaluation criteria.

(Evaluation Criteria) A: The right cheek had less dullness. B: The right cheek had obviously less dullness. C: The right cheek had a little less dullness. D: No difference is recognized between the left and right cheeks. E: The left cheek had obviously less dullness.

As a result, there were 19 subjects with A evaluation, 20 subjects with B evaluation and 1 subject with C evaluation, and 1 subject without D and E evaluations. When used, the liquid foundation obtained in Prescription Example 12 has an excellent effect of improving dullness because the amount of dullness is less than that in the case where the liquid foundation obtained in Comparative Prescription Example 3 is used. It turns out to be a thing.

In Example 6, none of the subjects had skin abnormality when the liquid foundation obtained in Formulation Example 12 was applied.

The liquid foundation obtained in Prescription Example 12 did not change its condition in one month.

[0116]

EFFECTS OF THE INVENTION The extract obtained by extracting cereals or refined residues of cereals with a neutral medium hardly inhibits the cell activity of the intracellular thyrodinase activity of cultured pigment cells, which is an index of the whitening effect. Without reducing the production of melanin,
Since the whitening cosmetic composition of the present invention containing such an extract exhibits the excellent action of suppressing pigmentation caused by ultraviolet irradiation at the same time, the whitening cosmetic composition of the present invention suppresses the expression of spots and freckles due to the accumulation of melanin, and the skin. The effect of improving the condition of, and maintaining white and beautiful skin with an improved dull feeling.

Furthermore, the whitening cosmetic composition of the present invention containing a decomposed product obtained by treating the extract with an enzyme exhibits a superior whitening effect.

Further, the whitening cosmetic composition of the present invention has the excellent whitening effect as described above, and also has the effect of being excellent in safety and storage stability on the skin and the like.

[Brief description of drawings]

FIG. 1 is a graph showing the relationship between the addition amount of the extract obtained in Preparation Example 3 and the production amount of dopachrome.

FIG. 2 is a graph showing the relationship between the amount of the extract obtained in Preparation Example 3 and the amount of NADH in mitochondria by the MTT reduction method.

FIG. 3 is a graph showing the relationship between the amount of the enzyme-decomposed product solution obtained in Preparation Example 4 and the amount of dopachrome produced.

FIG. 4 is a graph showing the relationship between the amount of the enzyme-decomposed product solution obtained in Preparation Example 4 and the amount of NADH in mitochondria by the MTT reduction method.

FIG. 5 is a graph showing the relationship between the addition amount of the enzymatic degradation product solution obtained in Preparation Example 4 and the production amount of melanin per 10 ⁶ cells.

FIG. 6 is a graph showing changes with time from the ultraviolet irradiation start date of the occupancy rate of each evaluation of the pigmentation suppression effect when the cream obtained in Prescription Example 1 is applied.

FIG. 7 is a graph showing the change over time from the ultraviolet irradiation start date of the occupancy rate for each evaluation of the pigmentation suppression effect when the cream obtained in Prescription Example 2 was applied.

FIG. 8 is a graph showing changes with time from the ultraviolet irradiation start date of the occupancy rate of each evaluation of the pigmentation suppressing effect when the cream obtained in Formulation Example 3 was applied.

FIG. 9 is a graph showing the change over time in the occupancy rate of each evaluation of the pigmentation suppression effect when the cream obtained in Formulation Example 4 was applied, from the ultraviolet irradiation start date.

Claims (4)

[Claims] 1. A whitening cosmetic composition comprising an extract obtained by extracting a grain or a refined residue of a grain with a neutral medium. 2. The whitening cosmetic composition according to claim 1, wherein the refined residue of the grains is rice bran or bran. 3. The whitening cosmetic composition according to claim 1, wherein a decomposed product obtained by treating the extract with an enzyme is blended. 4. An enzyme in which (A) actinase is combined with (B) at least one proteolytic enzyme selected from the group consisting of pepsins, trypsins, papains, peptidases and bromelain. Claim 3
Whitening cosmetics listed.