JPH0737978B2 - 生育可能細胞の標識 - Google Patents
生育可能細胞の標識Info
- Publication number
- JPH0737978B2 JPH0737978B2 JP62277256A JP27725687A JPH0737978B2 JP H0737978 B2 JPH0737978 B2 JP H0737978B2 JP 62277256 A JP62277256 A JP 62277256A JP 27725687 A JP27725687 A JP 27725687A JP H0737978 B2 JPH0737978 B2 JP H0737978B2
- Authority
- JP
- Japan
- Prior art keywords
- cells
- labeling
- cell
- dye
- good
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
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- 229960003699 evans blue Drugs 0.000 description 1
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- 238000002474 experimental method Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 125000001153 fluoro group Chemical group F* 0.000 description 1
- 238000010230 functional analysis Methods 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 150000002303 glucose derivatives Chemical class 0.000 description 1
- 230000005283 ground state Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 229960001855 mannitol Drugs 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000004264 monolayer culture Methods 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 231100001160 nonlethal Toxicity 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000001048 orange dye Substances 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 235000011056 potassium acetate Nutrition 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000003133 propidium iodide exclusion Methods 0.000 description 1
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000002165 resonance energy transfer Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 235000009518 sodium iodide Nutrition 0.000 description 1
- 229940083599 sodium iodide Drugs 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- OSQUFVVXNRMSHL-LTHRDKTGSA-M sodium;3-[(2z)-2-[(e)-4-(1,3-dibutyl-4,6-dioxo-2-sulfanylidene-1,3-diazinan-5-ylidene)but-2-enylidene]-1,3-benzoxazol-3-yl]propane-1-sulfonate Chemical compound [Na+].O=C1N(CCCC)C(=S)N(CCCC)C(=O)C1=C\C=C\C=C/1N(CCCS([O-])(=O)=O)C2=CC=CC=C2O\1 OSQUFVVXNRMSHL-LTHRDKTGSA-M 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
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- 238000004114 suspension culture Methods 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
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- 231100000331 toxic Toxicity 0.000 description 1
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- 238000012546 transfer Methods 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US925192 | 1986-10-31 | ||
| US06/925,192 US4783401A (en) | 1986-10-31 | 1986-10-31 | Viable cell labelling |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63122954A JPS63122954A (ja) | 1988-05-26 |
| JPH0737978B2 true JPH0737978B2 (ja) | 1995-04-26 |
Family
ID=25451357
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62277256A Expired - Lifetime JPH0737978B2 (ja) | 1986-10-31 | 1987-10-30 | 生育可能細胞の標識 |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US4783401A (da) |
| EP (1) | EP0266194B1 (da) |
| JP (1) | JPH0737978B2 (da) |
| KR (1) | KR970000783B1 (da) |
| CN (1) | CN1014547B (da) |
| AT (1) | ATE110417T1 (da) |
| AU (1) | AU606894B2 (da) |
| CA (1) | CA1311685C (da) |
| DE (1) | DE3750421T2 (da) |
| DK (1) | DK175074B1 (da) |
| IE (1) | IE69533B1 (da) |
| IL (1) | IL84278A (da) |
| PH (1) | PH23564A (da) |
| PT (1) | PT86028B (da) |
| ZA (1) | ZA878157B (da) |
Families Citing this family (61)
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| US4859584A (en) * | 1986-10-31 | 1989-08-22 | Smithkline Beckman Corporation | Cell growth rate determination by measurement of changes in cyanine dye levels in plasma membranes |
| ATE145337T1 (de) * | 1988-05-02 | 1996-12-15 | Phanos Tech Inc | Verbindungen, zusammensetzungen und verfahren zum binden von bio-affektions-substanzen an oberflächenmembranen von bioteilchen |
| US5256532A (en) * | 1988-05-02 | 1993-10-26 | Zynaxis Technologies, Inc. | Methods, reagents and test kits for determination of subpopulations of biological entities |
| US4963478A (en) * | 1988-07-05 | 1990-10-16 | Immucor, Inc. | Article for preforming immunological assays utilizing organic dyes and method for producing and utilizing same |
| JPH02187661A (ja) * | 1989-01-13 | 1990-07-23 | J M L:Kk | 網状赤血球の新染色法 |
| US5169788A (en) * | 1989-06-09 | 1992-12-08 | New England Deaconess Hospital Corporation | Methods of measuring membrane potential using j-aggregate forming dyes |
| US5279936A (en) * | 1989-12-22 | 1994-01-18 | Syntex (U.S.A.) Inc. | Method of separation employing magnetic particles and second medium |
| US5375606A (en) * | 1990-02-28 | 1994-12-27 | Zynaxis, Inc. | Bio-analytical separation method |
| US5242792A (en) * | 1991-02-25 | 1993-09-07 | The United States Of America As Represented By The Secretary Of The Navy | Method for the preservation of red blood cells by lyophilization using glycerol or inositol with disaccharides |
| WO1993002360A1 (en) * | 1991-07-16 | 1993-02-04 | Transmed Biotech Incorporated | Methods and compositions for simultaneous analysis of multiple analytes |
| EP0643706A1 (en) * | 1991-11-27 | 1995-03-22 | Zynaxis Inc. | Compounds, compositions and methods for binding bio-affecting substances to surface membranes of bio-particles |
| US5334509A (en) * | 1992-10-22 | 1994-08-02 | Riordan Neil H | Method for detecting intestinal pathogen dientamoeba fragilis |
| US5585246A (en) * | 1993-02-17 | 1996-12-17 | Biometric Imaging, Inc. | Method for preparing a sample in a scan capillary for immunofluorescent interrogation |
| AU6365894A (en) * | 1993-03-16 | 1994-10-11 | Alliance Pharmaceutical Corporation | Fluorocarbon compositions containing a visible or fluorescent label |
| ES2156927T3 (es) * | 1994-10-20 | 2001-08-01 | Sysmex Corp | Reactivo y metodo para analizar componentes solidos en orina. |
| US6004536A (en) * | 1995-11-14 | 1999-12-21 | Molecular Probes, Inc. | Lipophilic cyanine dyes with enchanced aqueous solubilty |
| US5804389A (en) * | 1995-12-29 | 1998-09-08 | Phanos Technologies, Inc. | Method for detecting abnormal epithelial cell shedding |
| US5989835A (en) * | 1997-02-27 | 1999-11-23 | Cellomics, Inc. | System for cell-based screening |
| US6008010A (en) * | 1996-11-01 | 1999-12-28 | University Of Pittsburgh | Method and apparatus for holding cells |
| US6727071B1 (en) | 1997-02-27 | 2004-04-27 | Cellomics, Inc. | System for cell-based screening |
| US7117098B1 (en) | 1997-02-27 | 2006-10-03 | Cellomics, Inc. | Machine-readable storage medium for analyzing distribution of macromolecules between the cell membrane and the cell cytoplasm |
| CA2282658C (en) | 1997-02-27 | 2003-02-25 | Cellomics, Inc. | A system for cell-based screening |
| US6858439B1 (en) * | 1999-03-15 | 2005-02-22 | Aviva Biosciences | Compositions and methods for separation of moieties on chips |
| EP1163372A4 (en) * | 1999-03-24 | 2002-10-02 | Princeton Separations | CHEMICALLY REACTIVE CYANINE DYES AND DERIVATIVES IN A LEVEL |
| US6986993B1 (en) * | 1999-08-05 | 2006-01-17 | Cellomics, Inc. | System for cell-based screening |
| WO2001042786A2 (en) | 1999-12-09 | 2001-06-14 | Cellomics, Inc. | System for cell based screening : cell spreading |
| US20090087450A1 (en) * | 2000-01-11 | 2009-04-02 | Greenville Hospital System | Combination therapy of hybrid cells with BCG injection for treating Cancer Patients |
| DK1248836T3 (da) | 2000-01-11 | 2005-08-15 | Greenville Hospital System | Fremgangsmåde til fremstilling af vacciner ved anvendelse af hybridceller |
| US20070212338A1 (en) * | 2000-01-11 | 2007-09-13 | Greenville Hospital System | Hybrid cells |
| US20030223935A1 (en) * | 2001-03-05 | 2003-12-04 | Gray Brian D. | Fluorescent membrane intercalating probes and methods for their use |
| AU2001245424B2 (en) * | 2000-03-03 | 2005-08-18 | Phanos Technologies, Inc. | Fluorescent membrane intercalating probes and methods for their use |
| WO2002008454A2 (en) * | 2000-07-24 | 2002-01-31 | Genprime, Inc. | Method and device for viable and nonviable prokaryotic and eukaryotic cell quantitation |
| WO2002012896A1 (en) * | 2000-08-08 | 2002-02-14 | Aviva Biosciences Corporation | Methods for manipulating moieties in microfluidic systems |
| US7309581B2 (en) * | 2000-11-01 | 2007-12-18 | Sysmex Corporation | Method of staining, detection and counting bacteria, and a diluent for bacterial stain |
| US6689391B2 (en) | 2001-03-30 | 2004-02-10 | Council Of Scientific & Industrial Research | Natural non-polar fluorescent dye from a non-bioluminescent marine invertebrate, compositions containing the said dye and its uses |
| US20020159625A1 (en) * | 2001-04-02 | 2002-10-31 | Cytoprint, Inc. | Method and apparatus for discovering, identifying and comparing biological activity mechanisms |
| EP1406492B1 (en) * | 2001-06-06 | 2009-12-30 | Perfusion Partners & Associates, Inc. | Centrifuge tube assembly |
| US6955872B2 (en) * | 2003-03-20 | 2005-10-18 | Coulter International Corp. | Dye compositions which provide enhanced differential fluorescence and light scatter characteristics |
| SI1656455T1 (sl) * | 2003-08-13 | 2012-12-31 | Sandoz Ag | Postopek za čiščenje rekombinantnih polipeptidov |
| US7776529B2 (en) | 2003-12-05 | 2010-08-17 | Life Technologies Corporation | Methine-substituted cyanine dye compounds |
| CA2560544C (en) | 2004-01-16 | 2015-05-19 | Carnegie Mellon University | Cellular labeling for nuclear magnetic resonance techniques |
| US10858384B2 (en) | 2004-03-22 | 2020-12-08 | Kode Biotech Limited | Synthetic molecule constructs |
| US7205145B2 (en) * | 2004-03-24 | 2007-04-17 | Zefon International, Inc. | Gas-borne matter collection device |
| US20050266415A1 (en) * | 2004-05-28 | 2005-12-01 | Zefon International, Inc. | Method for sampling gas-borne matter |
| US20060052324A1 (en) * | 2004-08-05 | 2006-03-09 | Artandi Steven E | Methods and compositions for cell activation |
| JP5306811B2 (ja) | 2005-05-11 | 2013-10-02 | ライフ テクノロジーズ コーポレーション | 2本鎖dnaへの高い選択性を有する蛍光化学物質及びそれらの使用 |
| AU2005234696B2 (en) | 2005-11-18 | 2012-02-16 | Phanos Technologies, Inc. | Fluorescent membrane intercalating probes and methods for their use |
| WO2008054509A2 (en) * | 2006-04-14 | 2008-05-08 | Celsense, Inc. | Methods for assessing cell labeling |
| WO2007120911A2 (en) * | 2006-04-14 | 2007-10-25 | Carnegie Mellon University | Cellular labeling and quantification for nuclear magnetic resonance techniques |
| ES2264403B1 (es) | 2006-06-22 | 2007-11-01 | Grifols S.A. | Medio de suspension de hematies. |
| US20100203637A1 (en) * | 2006-09-06 | 2010-08-12 | Elena Korchagina | Fluorescent cell markers |
| US7926368B2 (en) * | 2006-11-01 | 2011-04-19 | Zefon International, Inc. | Humidity-controlled gas-borne matter collection device |
| CA2693678C (en) * | 2007-07-10 | 2017-02-14 | Carnegie Mellon University | Compositions and methods for producing cellular labels for nuclear magnetic resonance techniques |
| US11266736B2 (en) | 2008-04-17 | 2022-03-08 | Vin De Bona Trading Company Pte Ltd | Method of painting micro vesicles |
| US20110110863A1 (en) * | 2008-05-02 | 2011-05-12 | Celsense, Inc. | Compositions and methods for producing emulsions for nuclear magnetic resonance techniques and other applications |
| DE102008040513B4 (de) * | 2008-07-17 | 2010-08-26 | BAM Bundesanstalt für Materialforschung und -prüfung | Verwendung einer langwellig emittierenden Cyaninverbindung als NIR-Fluoreszenzstandard und Kit zur Kalibrierung von Photolumineszenzmesssystemen |
| CN104685346B (zh) | 2012-05-02 | 2018-05-29 | 查尔斯河实验室公司 | 细胞捕获系统及其使用 |
| CA2872149C (en) | 2012-05-02 | 2019-03-19 | Charles River Laboratories, Inc. | Viability staining method |
| CN104755931B (zh) | 2012-05-02 | 2016-08-31 | 查尔斯河实验室公司 | 用于检测细胞样品中的活细胞的方法 |
| DK2906717T3 (da) | 2012-10-11 | 2019-04-01 | Vin De Bona Trading Company Pte Ltd | Fremgangsmåde til maling af mikrovesikler |
| EP4492037A1 (en) * | 2023-07-10 | 2025-01-15 | Sysmex Corporation | Method for detecting platelet aggregation |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4343782A (en) * | 1978-04-20 | 1982-08-10 | Shapiro Howard M | Cytological assay procedure |
| US4232121A (en) * | 1978-09-25 | 1980-11-04 | Eastman Kodak Company | Process for selecting methine dyes which inhibit cell growth |
| US4424201A (en) * | 1978-11-28 | 1984-01-03 | Rockefeller University | Employment of a mereyanine dye for the detection of malignant leukocytic cells |
| US4400370A (en) * | 1980-03-12 | 1983-08-23 | Lawrence Kass | Metachromatic dye sorption means for differential determination of leukocytes |
| US4581223A (en) * | 1980-03-12 | 1986-04-08 | Lawrence Kass | Individual leukocyte determination by means of differential metachromatic dye sorption |
| DE3238353A1 (de) * | 1982-10-15 | 1984-04-19 | Max Planck Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen | Verfahren zur simultanen quantitativen bestimmung der blutzellen und reagenz hierfuer |
| US4555396A (en) * | 1982-12-22 | 1985-11-26 | Eastman Kodak Company | Use of pyrylium and thiapyrylium compounds as biological stains |
| JPH06100596B2 (ja) * | 1986-09-10 | 1994-12-12 | 東亜医用電子株式会社 | フロ−サイトメトリ−による白血球の分類方法 |
-
1986
- 1986-10-31 US US06/925,192 patent/US4783401A/en not_active Expired - Lifetime
-
1987
- 1987-10-26 IL IL84278A patent/IL84278A/xx not_active IP Right Cessation
- 1987-10-26 CA CA000550187A patent/CA1311685C/en not_active Expired - Lifetime
- 1987-10-27 PH PH35993A patent/PH23564A/en unknown
- 1987-10-27 IE IE287287A patent/IE69533B1/en not_active IP Right Cessation
- 1987-10-28 AU AU80403/87A patent/AU606894B2/en not_active Ceased
- 1987-10-29 DE DE3750421T patent/DE3750421T2/de not_active Expired - Lifetime
- 1987-10-29 AT AT87309554T patent/ATE110417T1/de not_active IP Right Cessation
- 1987-10-29 PT PT86028A patent/PT86028B/pt not_active IP Right Cessation
- 1987-10-29 EP EP87309554A patent/EP0266194B1/en not_active Expired - Lifetime
- 1987-10-30 JP JP62277256A patent/JPH0737978B2/ja not_active Expired - Lifetime
- 1987-10-30 ZA ZA878157A patent/ZA878157B/xx unknown
- 1987-10-30 CN CN87107216A patent/CN1014547B/zh not_active Expired
- 1987-10-30 KR KR1019870012048A patent/KR970000783B1/ko not_active Expired - Fee Related
- 1987-10-30 DK DK198705703A patent/DK175074B1/da not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| ATE110417T1 (de) | 1994-09-15 |
| PT86028A (en) | 1987-11-01 |
| CN87107216A (zh) | 1988-05-11 |
| EP0266194B1 (en) | 1994-08-24 |
| IE69533B1 (en) | 1996-09-18 |
| EP0266194A2 (en) | 1988-05-04 |
| IE872872L (en) | 1988-04-30 |
| DE3750421T2 (de) | 1995-03-16 |
| DK570387D0 (da) | 1987-10-30 |
| US4783401A (en) | 1988-11-08 |
| KR880005456A (ko) | 1988-06-29 |
| DK175074B1 (da) | 2004-05-24 |
| EP0266194A3 (en) | 1988-08-03 |
| CA1311685C (en) | 1992-12-22 |
| ZA878157B (en) | 1988-08-31 |
| JPS63122954A (ja) | 1988-05-26 |
| DK570387A (da) | 1988-05-01 |
| KR970000783B1 (ko) | 1997-01-20 |
| IL84278A (en) | 1992-07-15 |
| AU8040387A (en) | 1988-05-05 |
| CN1014547B (zh) | 1991-10-30 |
| IL84278A0 (en) | 1988-03-31 |
| DE3750421D1 (de) | 1994-09-29 |
| PH23564A (en) | 1989-08-25 |
| AU606894B2 (en) | 1991-02-21 |
| PT86028B (pt) | 1990-08-31 |
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