JPH08238024A - Production of cortinellus shiitake chip spawn - Google Patents

Production of cortinellus shiitake chip spawn

Info

Publication number
JPH08238024A
JPH08238024A JP7070698A JP7069895A JPH08238024A JP H08238024 A JPH08238024 A JP H08238024A JP 7070698 A JP7070698 A JP 7070698A JP 7069895 A JP7069895 A JP 7069895A JP H08238024 A JPH08238024 A JP H08238024A
Authority
JP
Japan
Prior art keywords
shiitake
cortinellus shiitake
cortinellus
container
fungus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP7070698A
Other languages
Japanese (ja)
Inventor
Mitsuaki Okino
光明 沖野
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to JP7070698A priority Critical patent/JPH08238024A/en
Publication of JPH08238024A publication Critical patent/JPH08238024A/en
Pending legal-status Critical Current

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  • Mushroom Cultivation (AREA)

Abstract

PURPOSE: To produce a Cortinellus shiitake chip spawn considered in long period storage. CONSTITUTION: A Cortinellus shiitake culture medium 30 comprising the mixture of rice bran 31 with saw dust 32 is press-filled into an air-permeable flanged container 40 permitting the passage of Cortinellus shiitake fungus, and the container is covered and sealed with a synthetic film 43 to produce the storage type raw chip capsule 10 having the Cortinellus shiitake culture medium 30 sealed therein. When needed, the raw chip capsule 10 is discharged from the container, immersed in water, drained, thermally sterilized, cooled, and subsequently subjected to the adhesion of the original fungus 25 of the Cortinellus shiitake to the outer wall of the raw chip capsule 10 in an aseptic chamber 24. The Cortinellus shiitake fungus infiltrated into the Cortinellus shiitake culture medium 30 is cultured and bred to produce a Cortinellus shiitake chip spawn 11.

Description

【発明の詳細な説明】Detailed Description of the Invention

【0001】[0001]

【産業上の利用分野】この発明は、長期間の保存を考慮
した椎茸種駒の製造法に関するものである。
BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for manufacturing shiitake mushroom seed pieces in consideration of long-term storage.

【0002】[0002]

【従来の技術】木駒種菌駒を製造する時は、先ず原木を
直径8〜12ミリ、長さ15〜20ミリに切って作った
木駒を蒸気殺菌して、無菌室で椎茸原菌を入れ種菌木駒
を作る方法であるが、この方法は手間が掛かる。また鋸
屑と米糠を混合したものに、椎茸原菌を入れて作ったも
のもあるが、制限の多い無菌室で処理するため、培養に
時間がかかりロスも多く保存性がなかった。
2. Description of the Related Art When producing a wood piece, a wood piece is first sterilized by steam sterilizing a wood piece made by cutting a raw wood into a diameter of 8 to 12 mm and a length of 15 to 20 mm, and then producing a mushroom fungus in a sterile room. This is a method to make seed fungus wood pieces, but this method is troublesome. Also, there is a mixture of sawdust and rice bran mixed with Shiitake progenitor bacteria, but since it is processed in a sterile room with many restrictions, it takes a long time to culture and there is no loss of preservation.

【0003】[0003]

【発明が解決しようとする課題】種菌木駒を作る方法は
手間が掛かり、また鋸屑と米糠を混合したものに椎茸原
菌を入れたものは、雑菌が繁殖し易くロスの多いことで
ある。
[Problems to be Solved by the Invention] The method for producing the inoculum wood pieces is troublesome, and the mixture of sawdust and rice bran with Shiitake progenitors is prone to the proliferation of miscellaneous bacteria and a large loss.

【0004】[0004]

【課題を解決するための手段】図面を参考にして説明す
る。この発明に係る椎茸種駒の製造法は、米糠1重量部
に対して鋸屑8〜12重量部好ましくは10重量部混合
した混合物である椎茸育成媒体30を、直径8〜11ミ
リ・深さ15〜20ミリの椎茸菌通過可能程度の通気性
容器40中に加圧充填すると共に合成樹脂フイルム43
で被蓋封止して椎茸育成媒体30を封入した保存型原駒
カプセル10を製造し、必要に応じ長期間保存後取出し
た原駒カプセル10を、2時間浸水後、約20分水切り
したのち、120℃で40分間加熱殺菌後冷却してか
ら、無菌室で原駒カプセル10の外壁に椎茸原菌25を
付着させ、通気孔41を通じて椎茸育成媒体30中に椎
茸菌を侵入させ培養繁殖させてなる椎茸種駒の製造法で
ある。
A means for solving the problems will be described with reference to the drawings. The method for producing shiitake seed pieces according to the present invention is a mixture of shiitake growing medium 30 which is a mixture of 8 to 12 parts by weight, preferably 10 parts by weight of sawdust to 1 part by weight of rice bran, with a diameter of 8 to 11 mm and a depth of 15 to 15. A synthetic resin film 43 is filled with pressure in a breathable container 40 that allows passage of 20 mm of Shiitake bacterium.
After manufacturing the preservation type original piece capsule 10 in which the lid mushroom is sealed and the shiitake growing medium 30 is enclosed, if necessary, the original piece capsule 10 taken out after long-term storage is soaked for 2 hours and then drained for about 20 minutes. After heating and sterilizing at 120 ° C. for 40 minutes and then cooling, the Shiitake mushroom 25 is attached to the outer wall of the original piece capsule 10 in a sterile room, and the Shiitake mushroom is infiltrated into the Shiitake growing medium 30 through the ventilation hole 41 to culture and propagate. It is a method of manufacturing shiitake mushroom pieces.

【0005】[0005]

【実施例】米糠31:1重量部に対して鋸屑32:8〜
12重量部好ましくは10重量部混合した混合物を、直
径8〜11ミリ・深さ15〜20ミリの椎茸菌通過可能
程度の通気性通孔41を有する鍔42付容器40中に加
圧充填すると共に120℃で40分間の加熱に耐えられ
るポリエステル・ポリプロピレン等の合成樹脂フイルム
43を前記鍔42に熱融着封止して椎茸育成媒体30を
封入した保存型原駒カプセル10を製造する。必要に応
じ長期間保存後取出した、原駒カプセル10を、約2時
間水槽21中で浸水後、約20分水切り22したのち、
袋又は容器23に入れ120℃で40分間加熱殺菌室2
8内で加熱殺菌後冷却したのち、無菌室24内で原駒カ
プセル10の外壁に椎茸原菌25を付着させ、通気孔4
1を通じて椎茸育成媒体30中に椎茸菌を侵入させ、約
1箇月間培養繁殖させ、椎茸種駒11を製造する。
Example: Rice bran 31: 1 by weight to sawdust 32: 8-
12 parts by weight, preferably 10 parts by weight, of the mixed mixture is pressurized and filled into a container 40 with a collar 42 having a diameter of 8 to 11 mm and a depth of 15 to 20 mm and a breathable through hole 41 through which shiitake fungi can pass. At the same time, a synthetic resin film 43 of polyester, polypropylene or the like, which can withstand heating at 120 ° C. for 40 minutes, is heat-sealed and sealed to the collar 42 to manufacture the preservation type original piece capsule 10 in which the shiitake growing medium 30 is enclosed. If necessary, the original piece capsule 10 taken out after long-term storage is soaked in the water tank 21 for about 2 hours and then drained 22 for about 20 minutes.
Put in a bag or container 23 and heat sterilization chamber 2 at 120 ° C for 40 minutes
After heat sterilization in 8 and cooling, Shiitake bacterium 25 is attached to the outer wall of the original piece capsule 10 in the sterile room 24, and the vent hole 4
The shiitake fungus growing medium 30 is infiltrated through 1 and cultured and propagated for about 1 month to produce the shiitake seed piece 11.

【0006】[0006]

【作用】従来通り、原木にドリルで直径8〜13ミリ、
深さ10〜30ミリ程度の穴を開けておく。次に椎茸種
駒11を、原木の穴に詰めて植える。
[Function] As before, drilling a raw wood with a diameter of 8 to 13 mm,
Drill a hole with a depth of 10 to 30 mm. Next, the shiitake mushroom seed pieces 11 are packed in the holes of the raw wood and planted.

【0007】[0007]

【発明の効果】原木を切って木駒を作り椎茸菌を入れる
従来手段は、木駒を作るのに多大の時間を要するのと、
多量の原木ロスを生じること、木駒には殆ど栄養素を含
んでおらず、椎茸栽培に多時間要すること等の、欠点が
ある。
EFFECT OF THE INVENTION The conventional means for cutting wood to make wood pieces and inserting Shiitake fungi requires a great deal of time to make wood pieces,
There are drawbacks such as large amount of raw wood loss, wood pieces containing almost no nutrients, and many hours required for shiitake cultivation.

【0008】鋸屑と米糠を混合したものに、椎茸原菌を
入れて作ったものは、蓋材としてロウ或いは発泡スチロ
ールの板で、蓋をして湿気のある場所に寝かして、椎茸
に成長するのを待つ。しかしながら、原木の穴に詰め、
ロウ或いは発泡スチロールで、蓋をするのが難しく、雑
菌が混入し、繁殖し、椎茸菌の繁殖を妨害する危険性が
高いという欠点がある。
A mixture of sawdust and rice bran mixed with Shiitake progenitor is a wax or styrofoam plate as a lid material, which is laid in a humid place with a lid and grows into Shiitake mushrooms. Wait for However, filling the holes in the log,
The wax or styrofoam has the drawbacks that it is difficult to cover the lid and that there is a high risk that bacteria will mix in and proliferate and interfere with the growth of Shiitake fungi.

【0009】本発明に係る種駒だと、袋或いは容器から
取り出して、原木に開けてある穴に挿入するだけで良
く、簡単に植菌でき、時間も短縮できる。
With the seed piece according to the present invention, all that is required is to take it out of the bag or container and insert it into the hole opened in the raw wood, and it is possible to easily inoculate the bacteria and shorten the time.

【図面の簡単な説明】[Brief description of drawings]

【図1】この発明の製造過程図である。FIG. 1 is a manufacturing process diagram of the present invention.

【符号の説明】[Explanation of symbols]

10 原駒カプセル 11 椎茸種駒 21 水槽 22 水切り 23 袋・容器 24 無菌室 25 椎茸原菌 26 下型 27 プランジャー 28 加熱殺菌室 30 椎茸育成媒体 31 米糠 32 鋸屑 40 容器 41 通気孔 42 鍔 43 フイルム 10 Hara Koma Capsule 11 Shiitake Seed Piece 21 Water Tank 22 Drainer 23 Bags / Container 24 Aseptic Room 25 Shiitake Progenitor Bacteria 26 Lower Type 27 Plunger 28 Heat Sterilization Room 30 Shiitake Growth Medium 31 Rice Bran 32 Sawdust 40 Container 41 Vent 42 Muil 43 Fu

Claims (1)

【特許請求の範囲】[Claims] 【請求項1】 米糠1重量部に対して鋸屑8〜12重量
部好ましくは10重量部混合した混合物である椎茸育成
媒体(30)を、直径8〜11ミリ・深さ15〜20ミ
リの椎茸菌通過可能程度の通気性容器(40)中に加圧
充填すると共に合成樹脂フイルム(43)で被蓋封止し
て椎茸育成媒体(30)を封入した保存型原駒カプセル
(10)を製造し、必要に応じ長期間保存後取出した原
駒カプセル(10)を、2時間浸水後、約20分水切り
したのち、120℃で40分間加熱殺菌後冷却してか
ら、無菌室で原駒カプセル(10)の外壁に椎茸原菌
(25)を付着させ、通気孔(41)を通じて椎茸育成
媒体(30)中に椎茸菌を侵入させ培養繁殖させてなる
椎茸種駒の製造法。
1. A shiitake growing medium (30), which is a mixture of 8 to 12 parts by weight, and preferably 10 parts by weight of sawdust, to 1 part by weight of rice bran, and a shiitake having a diameter of 8 to 11 mm and a depth of 15 to 20 mm. Manufacture of a preservation-type original piece capsule (10) in which a mushroom-growing medium (30) is enclosed by press-filling in a breathable container (40) that allows passage of bacteria and sealing with a synthetic resin film (43). Then, if necessary, the original piece capsule (10) taken out after long-term storage is soaked in water for 2 hours, drained for about 20 minutes, sterilized by heating at 120 ° C for 40 minutes, and then cooled. A method for producing a shiitake mushroom seed piece, wherein shiitake mushroom fungus (25) is adhered to the outer wall of (10), and the shiitake mushroom is infiltrated into the shiitake mushroom-growing medium (30) through a vent hole (41) to culture and propagate the shiitake mushroom.
JP7070698A 1995-03-03 1995-03-03 Production of cortinellus shiitake chip spawn Pending JPH08238024A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP7070698A JPH08238024A (en) 1995-03-03 1995-03-03 Production of cortinellus shiitake chip spawn

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP7070698A JPH08238024A (en) 1995-03-03 1995-03-03 Production of cortinellus shiitake chip spawn

Publications (1)

Publication Number Publication Date
JPH08238024A true JPH08238024A (en) 1996-09-17

Family

ID=13439107

Family Applications (1)

Application Number Title Priority Date Filing Date
JP7070698A Pending JPH08238024A (en) 1995-03-03 1995-03-03 Production of cortinellus shiitake chip spawn

Country Status (1)

Country Link
JP (1) JPH08238024A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105027983A (en) * 2015-08-03 2015-11-11 黄旭胜 Bacterial strain interface perforator

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105027983A (en) * 2015-08-03 2015-11-11 黄旭胜 Bacterial strain interface perforator

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