JPH1048212A - 免疫クロマトグラフィー試験片を用いて分析対象物を測定する方法 - Google Patents

免疫クロマトグラフィー試験片を用いて分析対象物を測定する方法

Info

Publication number: JPH1048212A
Authority: JP; Japan
Prior art keywords: analyte; binding partner; label; test strip; fluid sample
Prior art date: 1996-05-31
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Pending

Application number

JP13622797A

Other languages

English (en)

Japanese (ja)

Inventor

Ronald G Sommer

ローランド・ジー・ソマー

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Bayer AG

Bayer Corp

Original Assignee

Bayer AG

Bayer Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1996-05-31

Filing date

1997-05-27

Publication date

1998-02-20

1997-05-27 Application filed by Bayer AG, Bayer Corp filed Critical Bayer AG

1998-02-20 Publication of JPH1048212A publication Critical patent/JPH1048212A/ja

Status Pending legal-status Critical Current

Links

238000012360 testing method Methods 0.000 title claims abstract description 93
239000012491 analyte Substances 0.000 title claims abstract description 92
238000000034 method Methods 0.000 title claims description 29
230000009870 specific binding Effects 0.000 claims abstract description 29
238000004458 analytical method Methods 0.000 claims abstract description 3
108091006905 Human Serum Albumin Proteins 0.000 claims description 61
102000008100 Human Serum Albumin Human genes 0.000 claims description 61
230000027455 binding Effects 0.000 claims description 50
239000012530 fluid Substances 0.000 claims description 38
239000010931 gold Substances 0.000 claims description 27
229910052737 gold Inorganic materials 0.000 claims description 26
PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 25
239000011159 matrix material Substances 0.000 claims description 21
230000009471 action Effects 0.000 claims description 12
239000000463 material Substances 0.000 claims description 9
239000002250 absorbent Substances 0.000 claims description 5
230000002745 absorbent Effects 0.000 claims description 5
238000006243 chemical reaction Methods 0.000 claims description 5
238000005259 measurement Methods 0.000 claims description 5
230000003100 immobilizing effect Effects 0.000 claims description 4
241000894007 species Species 0.000 claims description 4
238000003317 immunochromatography Methods 0.000 abstract 1
238000001514 detection method Methods 0.000 description 22
239000000243 solution Substances 0.000 description 16
238000002310 reflectometry Methods 0.000 description 14
102000004190 Enzymes Human genes 0.000 description 12
108090000790 Enzymes Proteins 0.000 description 12
239000004033 plastic Substances 0.000 description 9
229920003023 plastic Polymers 0.000 description 9
241000283707 Capra Species 0.000 description 8
230000000903 blocking effect Effects 0.000 description 8
239000003153 chemical reaction reagent Substances 0.000 description 7
239000012528 membrane Substances 0.000 description 7
239000000758 substrate Substances 0.000 description 7
210000002700 urine Anatomy 0.000 description 6
231100000673 dose–response relationship Toxicity 0.000 description 5
239000011534 wash buffer Substances 0.000 description 5
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
238000003556 assay Methods 0.000 description 4
239000007788 liquid Substances 0.000 description 4
108091003079 Bovine Serum Albumin Proteins 0.000 description 3
239000004677 Nylon Substances 0.000 description 3
239000000427 antigen Substances 0.000 description 3
102000036639 antigens Human genes 0.000 description 3
108091007433 antigens Proteins 0.000 description 3
229940098773 bovine serum albumin Drugs 0.000 description 3
239000005018 casein Substances 0.000 description 3
BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 3
235000021240 caseins Nutrition 0.000 description 3
238000005119 centrifugation Methods 0.000 description 3
239000010408 film Substances 0.000 description 3
238000003018 immunoassay Methods 0.000 description 3
230000006872 improvement Effects 0.000 description 3
230000005764 inhibitory process Effects 0.000 description 3
239000000203 mixture Substances 0.000 description 3
229920001778 nylon Polymers 0.000 description 3
230000000704 physical effect Effects 0.000 description 3
239000004372 Polyvinyl alcohol Substances 0.000 description 2
WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
230000008859 change Effects 0.000 description 2
238000011161 development Methods 0.000 description 2
230000000694 effects Effects 0.000 description 2
238000005516 engineering process Methods 0.000 description 2
238000002474 experimental method Methods 0.000 description 2
239000000706 filtrate Substances 0.000 description 2
230000005484 gravity Effects 0.000 description 2
238000002372 labelling Methods 0.000 description 2
239000000123 paper Substances 0.000 description 2
229920002451 polyvinyl alcohol Polymers 0.000 description 2
BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 2
238000002360 preparation method Methods 0.000 description 2
239000000047 product Substances 0.000 description 2
238000011002 quantification Methods 0.000 description 2
238000010992 reflux Methods 0.000 description 2
230000004044 response Effects 0.000 description 2
230000035945 sensitivity Effects 0.000 description 2
239000011780 sodium chloride Substances 0.000 description 2
238000003756 stirring Methods 0.000 description 2
239000000126 substance Substances 0.000 description 2
LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
241000588724 Escherichia coli Species 0.000 description 1
101001057156 Homo sapiens Melanoma-associated antigen C2 Proteins 0.000 description 1
102100027252 Melanoma-associated antigen C2 Human genes 0.000 description 1
229920003094 Methocel™ K4M Polymers 0.000 description 1
239000000020 Nitrocellulose Substances 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
229920001213 Polysorbate 20 Polymers 0.000 description 1
239000004699 Ultra-high molecular weight polyethylene Substances 0.000 description 1
230000002378 acidificating effect Effects 0.000 description 1
230000006978 adaptation Effects 0.000 description 1
239000000853 adhesive Substances 0.000 description 1
230000001070 adhesive effect Effects 0.000 description 1
238000002820 assay format Methods 0.000 description 1
229910021538 borax Inorganic materials 0.000 description 1
125000003636 chemical group Chemical group 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
238000009535 clinical urine test Methods 0.000 description 1
230000002860 competitive effect Effects 0.000 description 1
239000012141 concentrate Substances 0.000 description 1
230000008021 deposition Effects 0.000 description 1
239000006185 dispersion Substances 0.000 description 1
238000004090 dissolution Methods 0.000 description 1
229920001903 high density polyethylene Polymers 0.000 description 1
239000004700 high-density polyethylene Substances 0.000 description 1
229940127121 immunoconjugate Drugs 0.000 description 1
230000014759 maintenance of location Effects 0.000 description 1
229920001220 nitrocellulos Polymers 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
239000008055 phosphate buffer solution Substances 0.000 description 1
239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
229910000027 potassium carbonate Inorganic materials 0.000 description 1
239000001103 potassium chloride Substances 0.000 description 1
235000011164 potassium chloride Nutrition 0.000 description 1
229910000160 potassium phosphate Inorganic materials 0.000 description 1
235000011009 potassium phosphates Nutrition 0.000 description 1
230000008569 process Effects 0.000 description 1
238000000159 protein binding assay Methods 0.000 description 1
238000012205 qualitative assay Methods 0.000 description 1
238000012206 semi-quantitative assay Methods 0.000 description 1
239000001509 sodium citrate Substances 0.000 description 1
235000010339 sodium tetraborate Nutrition 0.000 description 1
238000012421 spiking Methods 0.000 description 1
239000010409 thin film Substances 0.000 description 1
230000007704 transition Effects 0.000 description 1
BSYLOTSXNQZYFW-UHFFFAOYSA-K trichlorogold;hydrate Chemical compound O.Cl[Au](Cl)Cl BSYLOTSXNQZYFW-UHFFFAOYSA-K 0.000 description 1
BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 description 1
HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
229940038773 trisodium citrate Drugs 0.000 description 1
229920000785 ultra high molecular weight polyethylene Polymers 0.000 description 1
238000000108 ultra-filtration Methods 0.000 description 1
230000000007 visual effect Effects 0.000 description 1
238000005406 washing Methods 0.000 description 1

Landscapes

Investigating Or Analysing Biological Materials (AREA)

JP13622797A 1996-05-31 1997-05-27 免疫クロマトグラフィー試験片を用いて分析対象物を測定する方法 Pending JPH1048212A (ja)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US65661396A	1996-05-31	1996-05-31
US08/656613		1996-05-31

Publications (1)

Publication Number	Publication Date
JPH1048212A true JPH1048212A (ja)	1998-02-20

Family

ID=24633803

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP13622797A Pending JPH1048212A (ja)	1996-05-31	1997-05-27	免疫クロマトグラフィー試験片を用いて分析対象物を測定する方法

Country Status (3)

Country	Link
JP (1)	JPH1048212A (fr)
AU (1)	AU2372597A (fr)
CA (1)	CA2198948A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US7993935B2 (en)	2005-01-06	2011-08-09	Sysmex Corporation	Test strip for immunochromatography

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB9814008D0 (en) *	1998-06-30	1998-08-26	Cambridge Sensors Ltd	Method for the analysis of fluids
AUPP713498A0 (en) *	1998-11-17	1998-12-10	Chandler, Howard Milne	A method of detecting blood
JP4562854B2 (ja)	2000-05-08	2010-10-13	パナソニック株式会社	クロマトグラフィー測定方法
JP5414424B2 (ja) *	2009-08-31	2014-02-12	シスメックス株式会社	目的物質の検出方法、及び目的物質を検出するためのクロマトグラフィー用試験キット

1997
- 1997-03-03 CA CA002198948A patent/CA2198948A1/fr not_active Abandoned
- 1997-05-27 JP JP13622797A patent/JPH1048212A/ja active Pending
- 1997-05-30 AU AU23725/97A patent/AU2372597A/en not_active Abandoned

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US7993935B2 (en)	2005-01-06	2011-08-09	Sysmex Corporation	Test strip for immunochromatography

Also Published As

Publication number	Publication date
AU2372597A (en)	1997-12-04
CA2198948A1 (fr)	1997-12-01

Publication	Publication Date	Title
US5569608A (en)	1996-10-29	Quantitative detection of analytes on immunochromatographic strips
US6436721B1 (en)	2002-08-20	Device and method for obtaining clinically significant analyte ratios
EP0138826B1 (fr)	1992-04-15	Detection de gonadotrophine chorionique humaine
JP4846573B2 (ja)	2011-12-28	基準としての天然分析物を有するラテラルフローアッセイ装置および方法
EP0465266B1 (fr)	1995-12-13	Immunoessai avec un signal visuel complémentaire
US6924153B1 (en)	2005-08-02	Quantitative lateral flow assays and devices
EP0447492B1 (fr)	1995-08-09	Appareil et procede de determination sequentielle d'un analyte dans un echantillon de liquide
AU2007319076B2 (en)	2013-10-17	Saturation assay
JPH04230857A (ja)	1992-08-19	結合アッセイ法および装置
US20040235189A1 (en)	2004-11-25	Reversed chromatographic immunoassay
CN101243320B (zh)	2012-11-14	采用带侧向迁移的免疫层析法的分析物检测
JP2013174612A (ja)	2013-09-05	凝集アッセイ
US20060246522A1 (en)	2006-11-02	C-reactive protein immunoassay and method
EP1540343B1 (fr)	2008-08-20	Methode pour eliminer l'interference dans des dosages immunochromatographiques
AU726976B2 (en)	2000-11-30	Formulation for reducing urea effect for immuno-chromatography assays using urine samples
JP4437211B2 (ja)	2010-03-24	免疫クロマトグラフィーを用いた測定方法およびそれに用いる検体分析用具
JPH1048212A (ja)	1998-02-20	免疫クロマトグラフィー試験片を用いて分析対象物を測定する方法
JP2001033453A (ja)	2001-02-09	リガンドの測定方法
US20050164405A1 (en)	2005-07-28	Non-specific "bridge" link specific "sandwich" immuno-complex to the solid phase in the lateral flow immunoassay
Monroe	1989	Enzyme Channelling Immunoassay (ECIA): A Unique and Rapid Quantitative Technique
JP2004511778A (ja)	2004-04-15	アッセイ方法およびそのためのキット

JPH1048212A - 免疫クロマトグラフィー試験片を用いて分析対象物を測定する方法 - Google Patents

Info

Links

Landscapes

Applications Claiming Priority (2)

Publications (1)

Family

ID=24633803

Family Applications (1)

Country Status (3)

Cited By (1)

Families Citing this family (4)

Cited By (1)

Also Published As

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