JPS5513002A - Preparation of urokinase - Google Patents
Preparation of urokinaseInfo
- Publication number
- JPS5513002A JPS5513002A JP7273078A JP7273078A JPS5513002A JP S5513002 A JPS5513002 A JP S5513002A JP 7273078 A JP7273078 A JP 7273078A JP 7273078 A JP7273078 A JP 7273078A JP S5513002 A JPS5513002 A JP S5513002A
- Authority
- JP
- Japan
- Prior art keywords
- urokinase
- sawdust
- adsorbent
- low
- tree
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000003990 Urokinase-type plasminogen activator Human genes 0.000 title abstract 7
- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 title abstract 7
- 229960005356 urokinase Drugs 0.000 title abstract 7
- 239000003463 adsorbent Substances 0.000 abstract 3
- 210000002700 urine Anatomy 0.000 abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 239000000428 dust Substances 0.000 abstract 1
- NAQMVNRVTILPCV-UHFFFAOYSA-N hexane-1,6-diamine Chemical compound NCCCCCCN NAQMVNRVTILPCV-UHFFFAOYSA-N 0.000 abstract 1
- 239000012535 impurity Substances 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: To separate and purify crude urokinase originated from human urine, using an easily available, low-cost adsorbent comprising sawdust.
CONSTITUTION: Pure urokinase is separated from human urine or crude urokinase derived therefrom, using an adsorbent comprising sawdust or a modified sawdust obtained by introducing diaminohexane, etc. to sawdust. The adsorbent specifically adsorbs a urokinase component having an estimated molecular weight of 50,000, and the adsorbed urokinase can be easily eluted with a dilute saline water or a buffer solution having specific ionic strength. The impurities in the urokinase can be reduced to several thousandth by the treatment. Preferably, the saw dust is produced from a tree of low rosin-content (e.g. low-terpene tree).
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7273078A JPS6050436B2 (en) | 1978-06-17 | 1978-06-17 | Production method of urokinase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7273078A JPS6050436B2 (en) | 1978-06-17 | 1978-06-17 | Production method of urokinase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5513002A true JPS5513002A (en) | 1980-01-29 |
| JPS6050436B2 JPS6050436B2 (en) | 1985-11-08 |
Family
ID=13497757
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7273078A Expired JPS6050436B2 (en) | 1978-06-17 | 1978-06-17 | Production method of urokinase |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6050436B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS57198452A (en) * | 1981-05-30 | 1982-12-06 | Dainippon Printing Co Ltd | Formation of colored picture |
-
1978
- 1978-06-17 JP JP7273078A patent/JPS6050436B2/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS57198452A (en) * | 1981-05-30 | 1982-12-06 | Dainippon Printing Co Ltd | Formation of colored picture |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6050436B2 (en) | 1985-11-08 |
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