JPS5571A - Lllysine oxidase - Google Patents
Lllysine oxidaseInfo
- Publication number
- JPS5571A JPS5571A JP7344178A JP7344178A JPS5571A JP S5571 A JPS5571 A JP S5571A JP 7344178 A JP7344178 A JP 7344178A JP 7344178 A JP7344178 A JP 7344178A JP S5571 A JPS5571 A JP S5571A
- Authority
- JP
- Japan
- Prior art keywords
- lysine
- oxidase
- producing
- lllysine
- lysinosis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004316 Oxidoreductases Human genes 0.000 title 1
- 108090000854 Oxidoreductases Proteins 0.000 title 1
- 108010004733 L-lysine oxidase Proteins 0.000 abstract 3
- 238000001914 filtration Methods 0.000 abstract 2
- GWENQMVPLJAMAE-UHFFFAOYSA-N 6-amino-2-oxohexanoic acid Chemical compound [NH3+]CCCCC(=O)C([O-])=O GWENQMVPLJAMAE-UHFFFAOYSA-N 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108010008292 L-Amino Acid Oxidase Proteins 0.000 abstract 1
- 102000007070 L-amino-acid oxidase Human genes 0.000 abstract 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 abstract 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 abstract 1
- 239000004472 Lysine Substances 0.000 abstract 1
- 229920005654 Sephadex Polymers 0.000 abstract 1
- 239000012507 Sephadex™ Substances 0.000 abstract 1
- 241000223259 Trichoderma Species 0.000 abstract 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 210000004748 cultured cell Anatomy 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 238000003745 diagnosis Methods 0.000 abstract 1
- 235000013305 food Nutrition 0.000 abstract 1
- 235000018977 lysine Nutrition 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 238000007833 oxidative deamination reaction Methods 0.000 abstract 1
- 229910052760 oxygen Inorganic materials 0.000 abstract 1
- 239000001301 oxygen Substances 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- WPLOVIFNBMNBPD-ATHMIXSHSA-N subtilin Chemical compound CC1SCC(NC2=O)C(=O)NC(CC(N)=O)C(=O)NC(C(=O)NC(CCCCN)C(=O)NC(C(C)CC)C(=O)NC(=C)C(=O)NC(CCCCN)C(O)=O)CSC(C)C2NC(=O)C(CC(C)C)NC(=O)C1NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C1NC(=O)C(=C/C)/NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C2NC(=O)CNC(=O)C3CCCN3C(=O)C(NC(=O)C3NC(=O)C(CC(C)C)NC(=O)C(=C)NC(=O)C(CCC(O)=O)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(N)CC=4C5=CC=CC=C5NC=4)CSC3)C(C)SC2)C(C)C)C(C)SC1)CC1=CC=CC=C1 WPLOVIFNBMNBPD-ATHMIXSHSA-N 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
NEW MATERIAL:L-lysine α-oxidase which is an L-amino acid oxidase capable of producing α-keto-ε-aminocaproic acid, NH3 and H2O2, having low Km value to L-lysine, high substrate specificity, and a molecular weight of about 112,000 measured by sephadex G-200 filtration.
USE: Determination of lysine in foods and drinks; diagnosis of lysinosis, etc., biochemical reagent, etc.
PROCESS: An electrophoretically pure L-lysine α-oxidase is prepared by (1) culturing microorganisms belonging to Trichoderma genus and capable of producing L-lysine α-oxidase, (2) extracting the cultured cells with water, and (3) purifying the resulting crude enzyme solution by filtering or dialyzing in the presence of oxygen, thereby effecting the oxidative deamination of the product.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7344178A JPS5571A (en) | 1978-06-16 | 1978-06-16 | Lllysine oxidase |
| GB7905850A GB2015532B (en) | 1978-02-27 | 1979-02-19 | -lysine -oxidase |
| DE19792906737 DE2906737C2 (en) | 1978-02-27 | 1979-02-21 | L-lysine α-oxidase, method for obtaining it and its use |
| DE19792954471 DE2954471C2 (en) | 1978-02-27 | 1979-02-21 | |
| US06/015,179 US4234691A (en) | 1978-02-27 | 1979-02-26 | L-Lysine α-oxidase |
| CA322,273A CA1111790A (en) | 1978-02-27 | 1979-02-26 | L-LYSINE .alpha.-OXIDASE |
| FR7904967A FR2418239A1 (en) | 1978-02-27 | 1979-02-27 | L-LYSINE A-OXYDASE, ITS PREPARATION AND USE FOR THE DETERMINATION OF L-LYSINE AND AS A THERAPEUTIC AGENT |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7344178A JPS5571A (en) | 1978-06-16 | 1978-06-16 | Lllysine oxidase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5571A true JPS5571A (en) | 1980-01-05 |
| JPS5756355B2 JPS5756355B2 (en) | 1982-11-29 |
Family
ID=13518322
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7344178A Granted JPS5571A (en) | 1978-02-27 | 1978-06-16 | Lllysine oxidase |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5571A (en) |
-
1978
- 1978-06-16 JP JP7344178A patent/JPS5571A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5756355B2 (en) | 1982-11-29 |
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