JPS5588694A - Agitator with immobilized biologically active protein - Google Patents
Agitator with immobilized biologically active proteinInfo
- Publication number
- JPS5588694A JPS5588694A JP16490178A JP16490178A JPS5588694A JP S5588694 A JPS5588694 A JP S5588694A JP 16490178 A JP16490178 A JP 16490178A JP 16490178 A JP16490178 A JP 16490178A JP S5588694 A JPS5588694 A JP S5588694A
- Authority
- JP
- Japan
- Prior art keywords
- poly
- amino acid
- agitator
- mixture
- biologically active
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
PURPOSE: To make uniform a reaction system with immobilized enzymes themselves without attaching air bubbles to the surface of the enzyme by immobilizing enzymes, on a poly(amino acid) used on the surface of the agitator.
CONSTITUTION: A silicone adhesive and chloroform are uniformly mixed with a poly(amino acid), e.g. poly(γ-methyl glutamate), having acid amino acid units, with agitation, and glass beads of 80W120 mesh are suspended in the mixture. An agitator is dipped in the mixture, dried, and reacted with hydazine hydrate and methanol to form crosslinking of -CONHNHCO- between the ester residues of the poly(amino acid) polymer. The treated agitator is then immersed in an activated enzyme solution to give the surface comprising the poly(amino acid) having immobilized enzymes thereon.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16490178A JPS5588694A (en) | 1978-12-27 | 1978-12-27 | Agitator with immobilized biologically active protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16490178A JPS5588694A (en) | 1978-12-27 | 1978-12-27 | Agitator with immobilized biologically active protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5588694A true JPS5588694A (en) | 1980-07-04 |
Family
ID=15802013
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP16490178A Pending JPS5588694A (en) | 1978-12-27 | 1978-12-27 | Agitator with immobilized biologically active protein |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5588694A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63126485A (en) * | 1986-11-18 | 1988-05-30 | Dentaru Kagaku Kk | Production of immobilized enzyme |
-
1978
- 1978-12-27 JP JP16490178A patent/JPS5588694A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63126485A (en) * | 1986-11-18 | 1988-05-30 | Dentaru Kagaku Kk | Production of immobilized enzyme |
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