JPS57166998A - Determination of cholinesterase activity - Google Patents

Determination of cholinesterase activity

Info

Publication number
JPS57166998A
JPS57166998A JP5170681A JP5170681A JPS57166998A JP S57166998 A JPS57166998 A JP S57166998A JP 5170681 A JP5170681 A JP 5170681A JP 5170681 A JP5170681 A JP 5170681A JP S57166998 A JPS57166998 A JP S57166998A
Authority
JP
Japan
Prior art keywords
nadh
hydroxybenzoic acid
cholinesterase
hydroxybenzoylcholine
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP5170681A
Other languages
Japanese (ja)
Other versions
JPS603837B2 (en
Inventor
Yoshihiro Ashihara
Yasushi Kasahara
Masami Sugiyama
Takahiro Harada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujirebio Inc
Original Assignee
Fujirebio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujirebio Inc filed Critical Fujirebio Inc
Priority to JP5170681A priority Critical patent/JPS603837B2/en
Publication of JPS57166998A publication Critical patent/JPS57166998A/en
Publication of JPS603837B2 publication Critical patent/JPS603837B2/en
Expired legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To determine the activity of the titled substance, by adding a solution containing hydroxybenzoylcholine, o-hydroxybenzoic acid hydroxylase and NADH to the specimen, and determining the light absorbance of the consumed NADH by colorimetry.
CONSTITUTION: The hydroxybenzoylchloline of formula is used as the synthetic substrate, and a solution containing hydroxybenzoylcholine, o-hydroxybenzoic acid hydroxylase and NADH is added to a liquid containing cholinesterase. The o-hydroxybenzoic acid produced from the hydroxybenzoylcholine by the action of the cholinesterase is converted to catechol by the action of the o-hydroxybenzoic acid hydroxylase in the presence of NADH. The amount of the consumed NADH is determined as the decrease of the light absorbance of NADH by colorimetry, and the cholinesterase activity is calculated from the value.
COPYRIGHT: (C)1982,JPO&Japio
JP5170681A 1981-04-08 1981-04-08 Method for measuring cholinesterase activity Expired JPS603837B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP5170681A JPS603837B2 (en) 1981-04-08 1981-04-08 Method for measuring cholinesterase activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP5170681A JPS603837B2 (en) 1981-04-08 1981-04-08 Method for measuring cholinesterase activity

Publications (2)

Publication Number Publication Date
JPS57166998A true JPS57166998A (en) 1982-10-14
JPS603837B2 JPS603837B2 (en) 1985-01-30

Family

ID=12894334

Family Applications (1)

Application Number Title Priority Date Filing Date
JP5170681A Expired JPS603837B2 (en) 1981-04-08 1981-04-08 Method for measuring cholinesterase activity

Country Status (1)

Country Link
JP (1) JPS603837B2 (en)

Also Published As

Publication number Publication date
JPS603837B2 (en) 1985-01-30

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