JPS5758892A - Determining method of free fatty acid - Google Patents
Determining method of free fatty acidInfo
- Publication number
- JPS5758892A JPS5758892A JP13294580A JP13294580A JPS5758892A JP S5758892 A JPS5758892 A JP S5758892A JP 13294580 A JP13294580 A JP 13294580A JP 13294580 A JP13294580 A JP 13294580A JP S5758892 A JPS5758892 A JP S5758892A
- Authority
- JP
- Japan
- Prior art keywords
- acyl
- coa
- coenzyme
- reaction system
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To complete the first reaction rapidly in kreacting an acyl coenzyme A synthetase (acyl CoA synthetase) with the titled substance in the presence of adenosine triphosphate (ATP) and a coenzyme A (CoA) in the first reaction system, and reacting an enzyme with the resultant acyl CoA in the second reaction system to produce hydrogen peroxide, by adding a pyrophosphatase to the first reaction system.
CONSTITUTION: A free fatty acid is reacted with an acyl coenzyme synthetase (acyl CoA sybthetase) in the presence of adenosine triphosphoate (ATP) and a coenzyme A (CoA) to form an acyl coenzyme A (acyl CoA) in the first reaction system. A pyrophosphatase is then added to the system as the second reaction, and the formed pyrophosphoric acid is converted into orthophosphoric acid, which is then rapidly removed from the reaction system to complete the reaction rapidly. The resultant acyl CoA is then reacted with an acyl CoA oxidase to form hydrogen peroxide, which is then reacted with an acyl CoA oxidase to form hydrogen peroxide, which is then colored and determined by the coloring method. Thus, the free fatty acid is determined.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13294580A JPS5758892A (en) | 1980-09-26 | 1980-09-26 | Determining method of free fatty acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13294580A JPS5758892A (en) | 1980-09-26 | 1980-09-26 | Determining method of free fatty acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5758892A true JPS5758892A (en) | 1982-04-08 |
Family
ID=15093163
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP13294580A Pending JPS5758892A (en) | 1980-09-26 | 1980-09-26 | Determining method of free fatty acid |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5758892A (en) |
-
1980
- 1980-09-26 JP JP13294580A patent/JPS5758892A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JPS5729297A (en) | Determining method of hydrogen peroxide and determining composition | |
| DE3060087D1 (en) | Method and reagent for the determination of creatine kinase | |
| GB1491560A (en) | Reagent composition and method for determination of glycerol concentrations | |
| JPS5685300A (en) | Determination of free fatty acid | |
| IE42572L (en) | Process for enzymatic analysis | |
| JPS5599194A (en) | Production of aldonic acid by enzyme method | |
| JPS57110198A (en) | Determination of choline esterase activity | |
| JPS5329994A (en) | Continuous reaction using enzymes or microorganisms | |
| GR3025741T3 (en) | Enzyme assays | |
| JPS5648895A (en) | Measurement of nonelectron pair bond fatty acid | |
| JPS5543409A (en) | Estimation method of l-lysine | |
| JPS5771398A (en) | Measuring method of blood serumal component | |
| EP0316791A3 (en) | 1-naphtolphtaleine mono phosphate, process for its preparation as well as its use for the determination of alcaline phosphatases | |
| JPS5596099A (en) | Production of s-adenosyl-l-methionine using enzyme | |
| JPS5743699A (en) | Measuring method of free fatty acid | |
| JPS5513068A (en) | Analysis kit and its method by the use of pyruvic acid oxidase | |
| JPS6420099A (en) | Method for determining free fatty acid and reagent composition for determining free fatty acid useful in said determination | |
| JPS56158097A (en) | Quantitative determination of free fatty acid | |
| JPS5711999A (en) | Novel adenosine phosphate compound containing introduced iodine and its preparation | |
| JPS5596100A (en) | Method of determination of free fatty acid | |
| JPS5645199A (en) | Reagent composition for determination of free fatty acid | |
| JPS56117797A (en) | Preparation of nicotinamide adenine dinucleotide phosphate | |
| JPS53118592A (en) | Preparation of high purity adenosine triphosphoric acid aqueous solution | |
| JPS5637557A (en) | Quantitative determination method of hydrogen peroxide | |
| JPS5774096A (en) | Measurement of enzyme activity |