JPS59210887A - Ｄｎａ断片及びその調製法 - Google Patents

Ｄｎａ断片及びその調製法

Info

Publication number: JPS59210887A
Authority: JP; Japan
Prior art keywords: restriction enzyme; megadaltons; plasmid; fragment; dna
Prior art date: 1983-04-28
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Granted

Application number

JP7556083A

Other languages

English (en)

Japanese (ja)

Other versions

JPH0441996B2 (fr

Inventor

Takanosuke Sano

佐野　孝之輔

Koichi Ito

宏一伊藤

Kiyoshi Miwa

清志三輪

Shigeru Nakamori

茂中森

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Ajinomoto Co Inc

Original Assignee

Ajinomoto Co Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1983-04-28

Filing date

1983-04-28

Publication date

1984-11-29

1983-04-28 Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc

1983-04-28 Priority to JP7556083A priority Critical patent/JPS59210887A/ja

1984-11-29 Publication of JPS59210887A publication Critical patent/JPS59210887A/ja

1992-07-10 Publication of JPH0441996B2 publication Critical patent/JPH0441996B2/ja

Status Granted legal-status Critical Current

Links

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241000894006 Bacteria Species 0.000 claims abstract description 22
241000186146 Brevibacterium Species 0.000 claims abstract description 15
108090000623 proteins and genes Proteins 0.000 claims abstract description 8
108030003594 Diaminopimelate decarboxylases Proteins 0.000 claims abstract description 7
239000013612 plasmid Substances 0.000 claims description 84
108091008146 restriction endonucleases Proteins 0.000 claims description 55
238000003776 cleavage reaction Methods 0.000 claims description 33
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238000000034 method Methods 0.000 claims description 25
239000002253 acid Substances 0.000 claims description 12
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239000004220 glutamic acid Substances 0.000 claims description 9
WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 7
239000013611 chromosomal DNA Substances 0.000 claims description 7
235000013922 glutamic acid Nutrition 0.000 claims description 7
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GMKMEZVLHJARHF-UHFFFAOYSA-N (2R,6R)-form-2.6-Diaminoheptanedioic acid Natural products OC(=O)C(N)CCCC(N)C(O)=O GMKMEZVLHJARHF-UHFFFAOYSA-N 0.000 description 3
235000019766 L-Lysine Nutrition 0.000 description 3
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239000002202 Polyethylene glycol Substances 0.000 description 3
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239000000047 product Substances 0.000 description 3
239000006228 supernatant Substances 0.000 description 3
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
JUQLUIFNNFIIKC-UHFFFAOYSA-N 2-aminopimelic acid Chemical compound OC(=O)C(N)CCCCC(O)=O JUQLUIFNNFIIKC-UHFFFAOYSA-N 0.000 description 2
241001517013 Calidris pugnax Species 0.000 description 2
102000016943 Muramidase Human genes 0.000 description 2
108010014251 Muramidase Proteins 0.000 description 2
108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 2
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
201000004283 Shwachman-Diamond syndrome Diseases 0.000 description 2
239000004473 Threonine Substances 0.000 description 2
238000004166 bioassay Methods 0.000 description 2
239000000872 buffer Substances 0.000 description 2
229940041514 candida albicans extract Drugs 0.000 description 2
230000002950 deficient Effects 0.000 description 2
VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 2
238000001493 electron microscopy Methods 0.000 description 2
239000000284 extract Substances 0.000 description 2
239000001963 growth medium Substances 0.000 description 2
238000010438 heat treatment Methods 0.000 description 2
229930027917 kanamycin Natural products 0.000 description 2
229960000318 kanamycin Drugs 0.000 description 2
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
229930182823 kanamycin A Natural products 0.000 description 2
239000007788 liquid Substances 0.000 description 2
229960000274 lysozyme Drugs 0.000 description 2
239000004325 lysozyme Substances 0.000 description 2
235000010335 lysozyme Nutrition 0.000 description 2
229930182817 methionine Natural products 0.000 description 2
239000002213 purine nucleotide Substances 0.000 description 2
150000003212 purines Chemical class 0.000 description 2
238000011160 research Methods 0.000 description 2
239000011780 sodium chloride Substances 0.000 description 2
229960002898 threonine Drugs 0.000 description 2
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical group OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
238000005406 washing Methods 0.000 description 2
239000012138 yeast extract Substances 0.000 description 2
AWRLKTYNEGEURZ-JCLMPDJQSA-N 2'-deamino-2'-hydroxyneamine Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](N)C[C@@H]1N AWRLKTYNEGEURZ-JCLMPDJQSA-N 0.000 description 1
108010000834 2-5A-dependent ribonuclease Proteins 0.000 description 1
102100027962 2-5A-dependent ribonuclease Human genes 0.000 description 1
241000186361 Actinobacteria <class> Species 0.000 description 1
239000004475 Arginine Substances 0.000 description 1
241000304886 Bacilli Species 0.000 description 1
101000798402 Bacillus licheniformis Ornithine racemase Proteins 0.000 description 1
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
241000595586 Coryne Species 0.000 description 1
241000186145 Corynebacterium ammoniagenes Species 0.000 description 1
102000012410 DNA Ligases Human genes 0.000 description 1
108010061982 DNA Ligases Proteins 0.000 description 1
206010059866 Drug resistance Diseases 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
241000588722 Escherichia Species 0.000 description 1
241000720950 Gluta Species 0.000 description 1
GRSZFWQUAKGDAV-KQYNXXCUSA-L IMP(2-) Chemical compound O[C@@H]1[C@H](O)[C@@H](COP([O-])([O-])=O)O[C@H]1N1C(N=CNC2=O)=C2N=C1 GRSZFWQUAKGDAV-KQYNXXCUSA-L 0.000 description 1
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
229930010555 Inosine Natural products 0.000 description 1
UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
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241000144155 Microbacterium ammoniaphilum Species 0.000 description 1
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241000567363 Puccinellia maritima Species 0.000 description 1
241000187561 Rhodococcus erythropolis Species 0.000 description 1
108010083644 Ribonucleases Proteins 0.000 description 1
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240000004808 Saccharomyces cerevisiae Species 0.000 description 1
241000251778 Squalus acanthias Species 0.000 description 1
239000004098 Tetracycline Substances 0.000 description 1
AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
239000007983 Tris buffer Substances 0.000 description 1
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AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
239000005557 antagonist Substances 0.000 description 1
239000003242 anti bacterial agent Substances 0.000 description 1
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
239000007853 buffer solution Substances 0.000 description 1
AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 1
239000001110 calcium chloride Substances 0.000 description 1
229910001628 calcium chloride Inorganic materials 0.000 description 1
150000001720 carbohydrates Chemical class 0.000 description 1
235000014633 carbohydrates Nutrition 0.000 description 1
230000008878 coupling Effects 0.000 description 1
238000010168 coupling process Methods 0.000 description 1
238000005859 coupling reaction Methods 0.000 description 1
238000012217 deletion Methods 0.000 description 1
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238000000432 density-gradient centrifugation Methods 0.000 description 1
229940079593 drug Drugs 0.000 description 1
239000003814 drug Substances 0.000 description 1
238000006911 enzymatic reaction Methods 0.000 description 1
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
229960005542 ethidium bromide Drugs 0.000 description 1
238000000605 extraction Methods 0.000 description 1
239000004744 fabric Substances 0.000 description 1
238000001502 gel electrophoresis Methods 0.000 description 1
230000012010 growth Effects 0.000 description 1
229960003786 inosine Drugs 0.000 description 1
238000003780 insertion Methods 0.000 description 1
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229940056360 penicillin g Drugs 0.000 description 1
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NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
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239000011734 sodium Substances 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
229940074404 sodium succinate Drugs 0.000 description 1
ZDQYSKICYIVCPN-UHFFFAOYSA-L sodium succinate (anhydrous) Chemical compound [Na+].[Na+].[O-]C(=O)CCC([O-])=O ZDQYSKICYIVCPN-UHFFFAOYSA-L 0.000 description 1
239000000758 substrate Substances 0.000 description 1
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235000019364 tetracycline Nutrition 0.000 description 1
150000003522 tetracyclines Chemical class 0.000 description 1
PIEPQKCYPFFYMG-UHFFFAOYSA-N tris acetate Chemical compound CC(O)=O.OCC(N)(CO)CO PIEPQKCYPFFYMG-UHFFFAOYSA-N 0.000 description 1
238000005199 ultracentrifugation Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)

Landscapes

Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Enzymes And Modification Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

JP7556083A 1983-04-28 1983-04-28 Ｄｎａ断片及びその調製法 Granted JPS59210887A (ja)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP7556083A JPS59210887A (ja)	1983-04-28	1983-04-28	Ｄｎａ断片及びその調製法

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP7556083A JPS59210887A (ja)	1983-04-28	1983-04-28	Ｄｎａ断片及びその調製法

Publications (2)

Publication Number	Publication Date
JPS59210887A true JPS59210887A (ja)	1984-11-29
JPH0441996B2 JPH0441996B2 (fr)	1992-07-10

Family

ID=13579681

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP7556083A Granted JPS59210887A (ja)	1983-04-28	1983-04-28	Ｄｎａ断片及びその調製法

Country Status (1)

Country	Link
JP (1)	JPS59210887A (fr)

1983
- 1983-04-28 JP JP7556083A patent/JPS59210887A/ja active Granted

Also Published As

Publication number	Publication date
JPH0441996B2 (fr)	1992-07-10

Publication	Publication Date	Title
JP2973446B2 (ja)	1999-11-08	新規プラスミドベクター
US4514502A (en)	1985-04-30	Composite plasmid
EP0082485B1 (fr)	1988-09-14	Vecteurs plasmidiques
EP0077548B1 (fr)	1989-01-18	Plasmide
JPH0138475B2 (fr)	1989-08-14
EP0158940B1 (fr)	1991-12-11	Plasmide composé transductible
EP0137348A2 (fr)	1985-04-17	ADN recombinant, bactéries contenant cet ADN recombinant et procédé de préparation de L-thréonine ou L-isoleucine utilisant ces bactéries
EP0124048B1 (fr)	1992-07-22	Procédé de production de L-tryptophane par fermentation
US5426050A (en)	1995-06-20	Plasmid vectors for expression of genes in coryneform bacteria
JPS59210887A (ja)	1984-11-29	Ｄｎａ断片及びその調製法
JP2903414B2 (ja)	1999-06-07	抗酸菌分泌発現ベクター及び抗酸菌
EP0215388B1 (fr)	1991-12-04	Vecteur de plasmide et une méthode de régulation d'expression de gène par utilisation de ce vecteur
EP0137387B1 (fr)	1991-09-04	Procédé de production de L-phénylanine par fermentation
US4778762A (en)	1988-10-18	Plasmid
JPH06102029B2 (ja)	1994-12-14	Ｌ−トリプトフアンの製造法
CN114539367B (zh)	2024-03-01	Cey17_rs11900基因突变体及其在制备l-缬氨酸中的应用
JPH0321154B2 (fr)	1991-03-22
JPS61216690A (ja)	1986-09-26	シヤトルベクタ−
CA1231314A (fr)	1988-01-12	Plasmides pour l'expression de genes etrangers dans b. subtilis
JPH0616713B2 (ja)	1994-03-09	複合プラスミド
JPH055479B2 (fr)	1993-01-22
JPH0734744B2 (ja)	1995-04-19	形質導入可能な複合プラスミド
JP2890526B2 (ja)	1999-05-17	新規なｄｎａ
JPH0734745B2 (ja)	1995-04-19	形質導入方法
JPH02472A (ja)	1990-01-05	Ｌ―グルタミン酸の製造方法