JPS5924386B2 - ポリヨ−ドチロニンの免疫学的分析法 - Google Patents

ポリヨ−ドチロニンの免疫学的分析法

Info

Publication number: JPS5924386B2
Authority: JP; Japan
Prior art keywords: ligand; usually; enz; added; enzyme
Prior art date: 1975-02-20
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

JP1571476A

Other languages

English (en)

Japanese (ja)

Other versions

JPS51106724A (ja

Inventor

ジユデイス・アイ・ブレイクモア

リチヤード・ケイ・ルート

ロバート・デイ・アーンスト

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Syva Co

Original Assignee

Syva Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1975-02-20

Filing date

1976-02-16

Publication date

1984-06-08

1976-02-16 Application filed by Syva Co filed Critical Syva Co

1976-09-21 Publication of JPS51106724A publication Critical patent/JPS51106724A/ja

1984-06-08 Publication of JPS5924386B2 publication Critical patent/JPS5924386B2/ja

Status Expired legal-status Critical Current

Links

238000004458 analytical method Methods 0.000 title description 13
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230000000694 effects Effects 0.000 claims description 26
102000013460 Malate Dehydrogenase Human genes 0.000 claims description 22
108010026217 Malate Dehydrogenase Proteins 0.000 claims description 22
239000003446 ligand Substances 0.000 claims description 22
125000004432 carbon atom Chemical group C* 0.000 claims description 21
238000000034 method Methods 0.000 claims description 18
229940034208 thyroxine Drugs 0.000 claims description 17
XUIIKFGFIJCVMT-UHFFFAOYSA-N thyroxine-binding globulin Natural products IC1=CC(CC([NH3+])C([O-])=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-UHFFFAOYSA-N 0.000 claims description 16
XUIIKFGFIJCVMT-GFCCVEGCSA-N D-thyroxine Chemical compound IC1=CC(C[C@@H](N)C(O)=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-GFCCVEGCSA-N 0.000 claims description 15
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 12
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229910052757 nitrogen Inorganic materials 0.000 claims description 7
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HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 8
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125000006413 ring segment Chemical group 0.000 description 7
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GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 6
125000003277 amino group Chemical group 0.000 description 6
239000012491 analyte Substances 0.000 description 6
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238000000502 dialysis Methods 0.000 description 6
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229940049920 malate Drugs 0.000 description 6
BJEPYKJPYRNKOW-UHFFFAOYSA-L malate(2-) Chemical compound [O-]C(=O)C(O)CC([O-])=O BJEPYKJPYRNKOW-UHFFFAOYSA-L 0.000 description 6
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KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 5
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239000005495 thyroid hormone Substances 0.000 description 5
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HZWZMEVCHUHUPY-HNNXBMFYSA-N 2-[[2-[[(2s)-3-[4-(4-hydroxy-3,5-diiodophenoxy)-3,5-diiodophenyl]-1-methoxy-1-oxopropan-2-yl]amino]-2-oxoethyl]amino]acetic acid Chemical compound IC1=CC(C[C@@H](C(=O)OC)NC(=O)CNCC(O)=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 HZWZMEVCHUHUPY-HNNXBMFYSA-N 0.000 description 1
FGQJBZHMORPBRR-UHFFFAOYSA-N 4-methylmorpholine-2,6-dione Chemical compound CN1CC(=O)OC(=O)C1 FGQJBZHMORPBRR-UHFFFAOYSA-N 0.000 description 1
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VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
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102100033495 Glycine dehydrogenase (decarboxylating), mitochondrial Human genes 0.000 description 1
101001056308 Homo sapiens Malate dehydrogenase, cytoplasmic Proteins 0.000 description 1
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239000004472 Lysine Substances 0.000 description 1
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
102100026475 Malate dehydrogenase, cytoplasmic Human genes 0.000 description 1
OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 1
201000004219 Meesmann corneal dystrophy Diseases 0.000 description 1
102000006746 NADH Dehydrogenase Human genes 0.000 description 1
108010086428 NADH Dehydrogenase Proteins 0.000 description 1
241000237988 Patellidae Species 0.000 description 1
240000004808 Saccharomyces cerevisiae Species 0.000 description 1
PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
102000009843 Thyroglobulin Human genes 0.000 description 1
108010034949 Thyroglobulin Proteins 0.000 description 1
241000251539 Vertebrata <Metazoa> Species 0.000 description 1
238000000862 absorption spectrum Methods 0.000 description 1
DHKHKXVYLBGOIT-UHFFFAOYSA-N acetaldehyde Diethyl Acetal Natural products CCOC(C)OCC DHKHKXVYLBGOIT-UHFFFAOYSA-N 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
230000000996 additive effect Effects 0.000 description 1
125000003545 alkoxy group Chemical group 0.000 description 1
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125000003368 amide group Chemical group 0.000 description 1
235000001014 amino acid Nutrition 0.000 description 1
150000001413 amino acids Chemical class 0.000 description 1
QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
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238000010171 animal model Methods 0.000 description 1
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239000012062 aqueous buffer Substances 0.000 description 1
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OHJMTUPIZMNBFR-UHFFFAOYSA-N biuret Chemical compound NC(=O)NC(N)=O OHJMTUPIZMNBFR-UHFFFAOYSA-N 0.000 description 1
210000004369 blood Anatomy 0.000 description 1
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239000012267 brine Substances 0.000 description 1
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238000006555 catalytic reaction Methods 0.000 description 1
239000007795 chemical reaction product Substances 0.000 description 1
NEHMKBQYUWJMIP-UHFFFAOYSA-N chloromethane Chemical compound ClC NEHMKBQYUWJMIP-UHFFFAOYSA-N 0.000 description 1
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238000010790 dilution Methods 0.000 description 1
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WPUMTJGUQUYPIV-JIZZDEOASA-L disodium (S)-malate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](O)CC([O-])=O WPUMTJGUQUYPIV-JIZZDEOASA-L 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
VHJLVAABSRFDPM-ZXZARUISSA-N dithioerythritol Chemical compound SC[C@H](O)[C@H](O)CS VHJLVAABSRFDPM-ZXZARUISSA-N 0.000 description 1
239000003480 eluent Substances 0.000 description 1
238000010828 elution Methods 0.000 description 1
238000007824 enzymatic assay Methods 0.000 description 1
CEIPQQODRKXDSB-UHFFFAOYSA-N ethyl 3-(6-hydroxynaphthalen-2-yl)-1H-indazole-5-carboximidate dihydrochloride Chemical compound Cl.Cl.C1=C(O)C=CC2=CC(C3=NNC4=CC=C(C=C43)C(=N)OCC)=CC=C21 CEIPQQODRKXDSB-UHFFFAOYSA-N 0.000 description 1
125000000816 ethylene group Chemical group [H]C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
238000001914 filtration Methods 0.000 description 1
239000012530 fluid Substances 0.000 description 1
239000006260 foam Substances 0.000 description 1
239000011888 foil Substances 0.000 description 1
150000003948 formamides Chemical class 0.000 description 1
238000004108 freeze drying Methods 0.000 description 1
238000001641 gel filtration chromatography Methods 0.000 description 1
239000008273 gelatin Substances 0.000 description 1
229920000159 gelatin Polymers 0.000 description 1
235000019322 gelatine Nutrition 0.000 description 1
235000011852 gelatine desserts Nutrition 0.000 description 1
238000002523 gelfiltration Methods 0.000 description 1
238000007429 general method Methods 0.000 description 1
239000011521 glass Substances 0.000 description 1
ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
239000000383 hazardous chemical Substances 0.000 description 1
231100000206 health hazard Toxicity 0.000 description 1
229910001385 heavy metal Inorganic materials 0.000 description 1
108060003552 hemocyanin Proteins 0.000 description 1
GNOIPBMMFNIUFM-UHFFFAOYSA-N hexamethylphosphoric triamide Chemical compound CN(C)P(=O)(N(C)C)N(C)C GNOIPBMMFNIUFM-UHFFFAOYSA-N 0.000 description 1
150000002430 hydrocarbons Chemical group 0.000 description 1
150000002431 hydrogen Chemical group 0.000 description 1
125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
150000002463 imidates Chemical class 0.000 description 1
125000005462 imide group Chemical group 0.000 description 1
238000010324 immunological assay Methods 0.000 description 1
230000002779 inactivation Effects 0.000 description 1
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239000000463 material Substances 0.000 description 1
239000002609 medium Substances 0.000 description 1
230000002503 metabolic effect Effects 0.000 description 1
XELZGAJCZANUQH-UHFFFAOYSA-N methyl 1-acetylthieno[3,2-c]pyrazole-5-carboxylate Chemical compound CC(=O)N1N=CC2=C1C=C(C(=O)OC)S2 XELZGAJCZANUQH-UHFFFAOYSA-N 0.000 description 1
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238000012986 modification Methods 0.000 description 1
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QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
125000000636 p-nitrophenyl group Chemical class [H]C1=C([H])C(=C([H])C([H])=C1*)[N+]([O-])=O 0.000 description 1
230000037361 pathway Effects 0.000 description 1
ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
125000003356 phenylsulfanyl group Chemical class [*]SC1=C([H])C([H])=C([H])C([H])=C1[H] 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
239000004033 plastic Substances 0.000 description 1
244000144977 poultry Species 0.000 description 1
239000002244 precipitate Substances 0.000 description 1
239000003755 preservative agent Substances 0.000 description 1
230000002335 preservative effect Effects 0.000 description 1
230000008569 process Effects 0.000 description 1
125000006239 protecting group Chemical group 0.000 description 1
230000035484 reaction time Effects 0.000 description 1
238000010992 reflux Methods 0.000 description 1
229920006395 saturated elastomer Polymers 0.000 description 1
238000013207 serial dilution Methods 0.000 description 1
235000019265 sodium DL-malate Nutrition 0.000 description 1
235000017557 sodium bicarbonate Nutrition 0.000 description 1
229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
239000001394 sodium malate Substances 0.000 description 1
229910052938 sodium sulfate Inorganic materials 0.000 description 1
235000011152 sodium sulphate Nutrition 0.000 description 1
POECFFCNUXZPJT-UHFFFAOYSA-M sodium;carbonic acid;hydrogen carbonate Chemical compound [Na+].OC(O)=O.OC([O-])=O POECFFCNUXZPJT-UHFFFAOYSA-M 0.000 description 1
238000004611 spectroscopical analysis Methods 0.000 description 1
239000003381 stabilizer Substances 0.000 description 1
239000011550 stock solution Substances 0.000 description 1
125000001424 substituent group Chemical group 0.000 description 1
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229960002175 thyroglobulin Drugs 0.000 description 1
238000004448 titration Methods 0.000 description 1
GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
235000002374 tyrosine Nutrition 0.000 description 1
150000003668 tyrosines Chemical class 0.000 description 1
235000012431 wafers Nutrition 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/78—Thyroid gland hormones, e.g. T3, T4, TBH, TBG or their receptors

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Immunology (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Chemical & Material Sciences (AREA)
Biomedical Technology (AREA)
Urology & Nephrology (AREA)
Hematology (AREA)
Biotechnology (AREA)
Analytical Chemistry (AREA)
Cell Biology (AREA)
Endocrinology (AREA)
Food Science & Technology (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

JP1571476A 1975-02-20 1976-02-16 ポリヨ−ドチロニンの免疫学的分析法 Expired JPS5924386B2 (ja)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US55156675A	1975-02-20	1975-02-20

Publications (2)

Publication Number	Publication Date
JPS51106724A JPS51106724A (ja)	1976-09-21
JPS5924386B2 true JPS5924386B2 (ja)	1984-06-08

Family

ID=24201792

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP1571476A Expired JPS5924386B2 (ja)	1975-02-20	1976-02-16	ポリヨ−ドチロニンの免疫学的分析法

Country Status (7)

Country	Link
JP (1)	JPS5924386B2 (it)
CA (1)	CA1028643A (it)
CH (1)	CH632343A5 (it)
DE (2)	DE2605162C2 (it)
FR (1)	FR2301823A1 (it)
GB (1)	GB1540728A (it)
IT (1)	IT1052946B (it)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4069105A (en) *	1977-03-03	1978-01-17	Syva Company	Lidocaine antigens and antibodies
IT1153999B (it) *	1977-03-15	1987-01-21	Snam Progetti	Composizione atta alla determinazione della triiodotironina e metodo diagnostico impiegante la stessa
US4156081A (en) *	1977-04-15	1979-05-22	Syva Company	Theophylline antigens and antibodies
GB2102946B (en) *	1981-06-30	1984-11-28	Wellcome Found	Enzyme immunoassay
EP0131096A2 (en) *	1983-05-02	1985-01-16	Miles Laboratories, Inc.	Homogeneous immunoassay method for determining binding substances and reagent system therefor
CA1317861C (en) *	1987-10-02	1993-05-18	Stephen B. Friedman	Homogeneous t-4 uptake assay
JP5465758B2 (ja)	2011-09-26	2014-04-09	富士フイルム株式会社	蛍光粒子を用いたサイロキシン免疫アッセイ
FR3146102B1 (fr)	2023-02-28	2025-01-10	Psa Automobiles Sa	Arrangement de recharge électrique pour véhicule automobile électrifié

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US3551296A (en) *	1968-06-11	1970-12-29	Calbiochem	Reagent and method for assaying malate dehydrogenase
US3817837A (en)	1971-05-14	1974-06-18	Syva Corp	Enzyme amplification assay
IL39323A (en) *	1971-05-14	1975-08-31	Syva Co	Highly sensitive assay method employing an enzyme for amplification
US3852157A (en) *	1971-05-14	1974-12-03	Syva Corp	Compounds for enzyme amplification assay

1975
- 1975-12-30 CA CA242,815A patent/CA1028643A/en not_active Expired
1976
- 1976-01-07 GB GB54276A patent/GB1540728A/en not_active Expired
- 1976-01-19 IT IT4767776A patent/IT1052946B/it active
- 1976-01-23 FR FR7601919A patent/FR2301823A1/fr active Granted
- 1976-02-09 CH CH150976A patent/CH632343A5/de not_active IP Right Cessation
- 1976-02-10 DE DE19762605162 patent/DE2605162C2/de not_active Expired
- 1976-02-10 DE DE19762660911 patent/DE2660911C2/de not_active Expired
- 1976-02-16 JP JP1571476A patent/JPS5924386B2/ja not_active Expired

Also Published As

Publication number	Publication date
GB1540728A (en)	1979-02-14
JPS51106724A (ja)	1976-09-21
DE2660911C2 (de)	1986-05-28
DE2605162C2 (de)	1986-08-28
CA1028643A (en)	1978-03-28
FR2301823A1 (fr)	1976-09-17
CH632343A5 (en)	1982-09-30
DE2605162A1 (de)	1976-09-02
IT1052946B (it)	1981-08-31
FR2301823B1 (it)	1980-05-23

Publication	Publication Date	Title
US4043872A (en)	1977-08-23	Polyiodothyronine immunoassay
US4190496A (en)	1980-02-26	Homogeneous enzyme assay for antibodies
US4134792A (en)	1979-01-16	Specific binding assay with an enzyme modulator as a labeling substance
US4067774A (en)	1978-01-10	Compounds for enzyme amplification assay
US3966556A (en)	1976-06-29	Compounds for enzyme amplification assay methadone analogs
US3843696A (en)	1974-10-22	Methadone analog compounds
US4214048A (en)	1980-07-22	Reagent suitable for enzyme immuno assay
US3905871A (en)	1975-09-16	Lactam conjugates to enzymes
US4171244A (en)	1979-10-16	Enzyme-bound-polyidothyronine
JPS58757A (ja)	1983-01-05	同位体を使用しない免疫検定を実施する方法、ラベルされた分析物とかかる検定に使用するキット
JPS59104554A (ja)	1984-06-16	螢光偏光技術を用いる不飽和チロキシン結合性蛋白位置の測定法
US4550163A (en)	1985-10-29	Ligand analog-irreversible enzyme inhibitor conjugates
US4043989A (en)	1977-08-23	Oxazepam derivatives for immunoassay reagents
JP2534989B2 (ja)	1996-09-18	ポリヨ−ドチロニンアツセイに有用なグルコ−ス−６−リン酸デヒドロゲナ−ゼコンジユゲ−ト
JP2727112B2 (ja)	1998-03-11	安定なペルオキシダーゼ組成物及び安定な抗体組成物
US3975237A (en)	1976-08-17	Compounds for enzyme amplification assay - - ecgonine analogs
US4376825A (en)	1983-03-15	Enzyme amplification compounds for assays for androgens
US4282325A (en)	1981-08-04	Enzyme bound corticosteroids
JPS5924386B2 (ja)	1984-06-08	ポリヨ−ドチロニンの免疫学的分析法
US5106950A (en)	1992-04-21	Polypeptide-labeled analyte analog for carrying out an immunoassay
JPH0460103B2 (it)	1992-09-25
Ullman et al.	1979	Mechanism of inhibition of malate dehydrogenase by thyroxine derivatives and reactivation by antibodies Homogeneous enzyme immunoassay for thryroxine
EP0043285B1 (en)	1984-01-04	Method for determination of valproic acid and reagents therein
US9777069B2 (en)	2017-10-03	Compositions and methods for detection of methadone metabolite
US10351830B2 (en)	2019-07-16	Conjugates for assays for oxycodone and oxymorphone