JPS6058074A - バチルス・ブレビスを形質転換する方法 - Google Patents

バチルス・ブレビスを形質転換する方法

Info

Publication number
JPS6058074A
JPS6058074A JP58165066A JP16506683A JPS6058074A JP S6058074 A JPS6058074 A JP S6058074A JP 58165066 A JP58165066 A JP 58165066A JP 16506683 A JP16506683 A JP 16506683A JP S6058074 A JPS6058074 A JP S6058074A
Authority
JP
Japan
Prior art keywords
plasmid
bacillus
bacillus brevis
plevis
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP58165066A
Other languages
English (en)
Japanese (ja)
Other versions
JPH0156756B2 (cs
Inventor
Juzo Udaka
重三 鵜高
Norihiro Tsukagoshi
規弘 塚越
Hideo Yamagata
山形　秀夫
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to JP58165066A priority Critical patent/JPS6058074A/ja
Publication of JPS6058074A publication Critical patent/JPS6058074A/ja
Publication of JPH0156756B2 publication Critical patent/JPH0156756B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP58165066A 1983-09-09 1983-09-09 バチルス・ブレビスを形質転換する方法 Granted JPS6058074A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP58165066A JPS6058074A (ja) 1983-09-09 1983-09-09 バチルス・ブレビスを形質転換する方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP58165066A JPS6058074A (ja) 1983-09-09 1983-09-09 バチルス・ブレビスを形質転換する方法

Publications (2)

Publication Number Publication Date
JPS6058074A true JPS6058074A (ja) 1985-04-04
JPH0156756B2 JPH0156756B2 (cs) 1989-12-01

Family

ID=15805214

Family Applications (1)

Application Number Title Priority Date Filing Date
JP58165066A Granted JPS6058074A (ja) 1983-09-09 1983-09-09 バチルス・ブレビスを形質転換する方法

Country Status (1)

Country Link
JP (1) JPS6058074A (cs)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2589880A1 (fr) * 1985-11-07 1987-05-15 Shigezo Udaka Procede pour l'expression de genes par bacillus brevis
EP0916733A1 (de) * 1997-11-17 1999-05-19 Roche Diagnostics GmbH Verfahren und Kit zur Klonierung von Fragmenten
EP0919623A3 (en) * 1997-11-17 1999-07-14 Roche Diagnostics GmbH Procedure and kit for cloning of DNA fragments
WO2005045005A1 (ja) 2003-11-11 2005-05-19 Higeta Shoyu Co., Ltd. 新規ブレビバチルス・チョウシネンシス及び該微生物を宿主とするタンパク質の製造方法
WO2006004067A1 (ja) * 2004-07-06 2006-01-12 Kaneka Corporation ブレビバチルス属細菌を用いたプロテインａ様蛋白質の生産方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5615300A (en) * 1979-05-11 1981-02-14 Gist Brocades Nv Plasmid and its use
JPS57132895A (en) * 1980-12-31 1982-08-17 Parubua Irutsuka Production of rearranged dna molecule and protein

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5615300A (en) * 1979-05-11 1981-02-14 Gist Brocades Nv Plasmid and its use
JPS57132895A (en) * 1980-12-31 1982-08-17 Parubua Irutsuka Production of rearranged dna molecule and protein

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2589880A1 (fr) * 1985-11-07 1987-05-15 Shigezo Udaka Procede pour l'expression de genes par bacillus brevis
US4994380A (en) * 1985-11-07 1991-02-19 Shigezo Udaka Process for expressing genes by Bacillus brevis
EP0916733A1 (de) * 1997-11-17 1999-05-19 Roche Diagnostics GmbH Verfahren und Kit zur Klonierung von Fragmenten
EP0919623A3 (en) * 1997-11-17 1999-07-14 Roche Diagnostics GmbH Procedure and kit for cloning of DNA fragments
WO2005045005A1 (ja) 2003-11-11 2005-05-19 Higeta Shoyu Co., Ltd. 新規ブレビバチルス・チョウシネンシス及び該微生物を宿主とするタンパク質の製造方法
US7655452B1 (en) 2003-11-11 2010-02-02 Higeta Shoyu Co., Ltd. Brevibacillus choshinensis and process for prodcuing protein wtih use of the microbe as host
JPWO2006004067A1 (ja) * 2004-07-06 2008-04-24 株式会社カネカ ブレビバチルス属細菌を用いたプロテインａ様蛋白質の生産方法
WO2006004067A1 (ja) * 2004-07-06 2006-01-12 Kaneka Corporation ブレビバチルス属細菌を用いたプロテインａ様蛋白質の生産方法
JP2010246569A (ja) * 2004-07-06 2010-11-04 Kaneka Corp ブレビバチルス属細菌を用いたプロテインａ様蛋白質の生産方法
EP2251425A1 (en) 2004-07-06 2010-11-17 Kaneka Corporation Process for producing protein A-like protein with use of Brevibacillus genus bacterium
US8597908B2 (en) 2004-07-06 2013-12-03 Kaneka Corporation Process for producing protein A-like protein with use of Brevibacillus genus bacterium
JP2014064589A (ja) * 2004-07-06 2014-04-17 Kaneka Corp ブレビバチルス属細菌を用いたプロテインａ様蛋白質の生産方法
JP2016063844A (ja) * 2004-07-06 2016-04-28 株式会社カネカ ブレビバチルス属細菌を用いたプロテインａ様蛋白質の生産方法

Also Published As

Publication number Publication date
JPH0156756B2 (cs) 1989-12-01

Similar Documents

Publication Publication Date Title
AU570709B2 (en) Cloning and expression in industrial species
US5217878A (en) Molecular cloning and expression of genes encoding proteolytic enzymes
AU620326B2 (en) Stable gene amplification in chromosomal dna of prokaryotic microorganisms
EP0686195B1 (en) Method and system for enhanced production of commercially important exoproteins in gram-positive bacteria
WO1984004541A1 (en) Expression and secretion vectors and method of constructing vectors
US5017477A (en) Enhancing DNA sequencies derived from the sacQ gene
JPS6058074A (ja) バチルス・ブレビスを形質転換する方法
US6083718A (en) Transformed industrial bacillus strains and methods for making and using them
Zaghloul et al. High level of expression and stability of the cloned alkaline protease (aprA) gene in Bacillus subtilis
GB2171703A (en) Expression-secretion vector
JPS62122589A (ja) Ｄｎａ塩基配列およびベクタ−
IE863080L (en) Expression in lactic acid bacteria
JP2620852B2 (ja) 新規プラスミド及びそれにより形質転換された新規微生物
JPH05284973A (ja) 組換プラスミドおよびこれをベクターとして用いる異種蛋白質の分泌生産方法
JP2500312B2 (ja) ヒト成長ホルモンの生産法
EP0186631B1 (en) Psm112 plasmid vector for expression in bacillus subtilis
JP2560214B2 (ja) 分泌シグナルペプチドをコードするｄｎａ配列
JP2537764B2 (ja) 高発現ベクタ―、バチルス属細菌、およびペプチド又は蛋白質の製造方法
JPS61166400A (ja) 蛋白生産の方法
JPS60145090A (ja) Ｄνａ
JPH0817702B2 (ja) 新規プラスミド及びその調製方法並びにそのプラスミドを含有する新規微生物
JPS61282084A (ja) 発現用ベクタ−
JPS61212288A (ja) 蛋白製造法
JPS63133986A (ja) 分泌シグナルペプチドをコ−ドするｄｎａ配列
JPS60203194A (ja) Ｄνａ