JPS63246169A - Oxygen permeable membrane - Google Patents
Oxygen permeable membraneInfo
- Publication number
- JPS63246169A JPS63246169A JP62080267A JP8026787A JPS63246169A JP S63246169 A JPS63246169 A JP S63246169A JP 62080267 A JP62080267 A JP 62080267A JP 8026787 A JP8026787 A JP 8026787A JP S63246169 A JPS63246169 A JP S63246169A
- Authority
- JP
- Japan
- Prior art keywords
- membrane
- oxygen
- permeable membrane
- oxygen permeable
- silk fibroin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000012528 membrane Substances 0.000 title claims description 38
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title claims description 25
- 239000001301 oxygen Substances 0.000 title claims description 25
- 229910052760 oxygen Inorganic materials 0.000 title claims description 25
- 108010022355 Fibroins Proteins 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 230000035699 permeability Effects 0.000 description 11
- 241000255789 Bombyx mori Species 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 210000004907 gland Anatomy 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 210000004087 cornea Anatomy 0.000 description 2
- 239000002178 crystalline material Substances 0.000 description 2
- 238000002425 crystallisation Methods 0.000 description 2
- 230000008025 crystallization Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 241000255794 Bombyx mandarina Species 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- 240000000249 Morus alba Species 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 210000002816 gill Anatomy 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000004584 polyacrylic acid Substances 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920006254 polymer film Polymers 0.000 description 1
- 229920005597 polymer membrane Polymers 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Eyeglasses (AREA)
- Materials For Medical Uses (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.
Description
【発明の詳細な説明】
゛〔技術分野〕
本発明は絹フィブロインからなる酸素透過膜に関するも
のである。DETAILED DESCRIPTION OF THE INVENTION [Technical Field] The present invention relates to an oxygen permeable membrane made of silk fibroin.
近年各種高分子膜が開発され、そのガス透過性、水蒸気
透過性、溶質透過性等を利用して各種分野に応用されて
いる。それらの高分子膜のうち、酸素透過性を有するも
のは、コンタクトレンズや、人工角膜、人工皮膚等に応
用されている。従来一般に知られている酸素透過膜は、
シリコーン系高分子やポリアクリル酸系高分子からなる
ものであるが、前者の場合、膜を薄く加工するのが困難
であり、また汚染されやすい等の問題があり、また後者
の場合、酸素透過性に問題があり、未だ満足し得るもの
ではなく、新しい素材のＷ１素透過膜の開発が望まれて
いる。In recent years, various polymer membranes have been developed and applied to various fields by utilizing their gas permeability, water vapor permeability, solute permeability, etc. Among these polymer films, those having oxygen permeability are applied to contact lenses, artificial corneas, artificial skin, and the like. Conventionally known oxygen permeable membranes are
They are made of silicone-based polymers or polyacrylic acid-based polymers, but in the case of the former, it is difficult to process the membrane thinly and there are problems such as easy contamination, and in the case of the latter, oxygen permeation is difficult. However, there are problems with the performance of the W1 element, and it is still unsatisfactory, and there is a desire to develop a W1 element permeable membrane made of a new material.
本発明は、前記の如き問題を含まない新しい素材からな
る酸素透過膜を提供することを目的とする。An object of the present invention is to provide an oxygen permeable membrane made of a new material that does not have the above-mentioned problems.
本発明によれば、絹フィブロイン膜からなり、酸膜は結
晶質を含み、水不溶性であることを特徴とする酸素透過
膜が提供される。According to the present invention, an oxygen permeable membrane is provided, which is made of a silk fibroin membrane, and is characterized in that the acid membrane contains crystalline material and is water-insoluble.
本発明の酸素透過膜は、絹フィブロイン水溶液を製膜し
、絹フィブロイン膜を得る。この膜は非晶質で水溶性を
示す。本発明では、この膜を結晶化し、その膜中に結晶
質を含有させ、水不溶性のものとする。絹フィブロイン
膜を結晶質化させるにはエタノールやメタノール等の絹
ブイブロインに対する貧溶媒を用いて行うことができる
。The oxygen permeable membrane of the present invention is obtained by forming a silk fibroin membrane from an aqueous silk fibroin solution. This film is amorphous and water-soluble. In the present invention, this film is crystallized to contain crystalline material and to be water-insoluble. Crystallization of the silk fibroin membrane can be carried out using a poor solvent for silk fibroin, such as ethanol or methanol.
原料として用いる絹フィブロインは、家蚕あるいは野蚕
由来のものでよい。また絹フィブロイン溶液は、熟蚕体
内の絹糸腺より取出した液状絹フィブロインを用いるこ
とができるし、生糸から再生した絹フィブロインを溶液
化したものでもよい。Silk fibroin used as a raw material may be derived from domestic silkworms or wild silkworms. Further, as the silk fibroin solution, liquid silk fibroin taken out from the silk gland in a mature silkworm body can be used, or a solution obtained by regenerating silk fibroin from raw silk may be used.
絹フィブロイン膜の結晶化処理は、溶媒と水からなる混
合溶液中で、絹フィブロイン膜を浸漬処理した後、乾燥
することによって実施することができる。この場合、混
合溶液の組成、温度、処理時間を変えることによって、
膜の酸素透過性を制御することができる。乾燥温度は５
〜８０℃である。The crystallization treatment of the silk fibroin membrane can be carried out by immersing the silk fibroin membrane in a mixed solution consisting of a solvent and water, and then drying the membrane. In this case, by changing the composition, temperature, and treatment time of the mixed solution,
The oxygen permeability of the membrane can be controlled. Drying temperature is 5
~80°C.
本発明において、絹フィブロイン膜を結晶化させて酸素
透過膜とする場合、その結晶化度は少なくとも１５％％
好ましくは２０〜８０％の範囲に規定するのがよい。In the present invention, when the silk fibroin membrane is crystallized to form an oxygen permeable membrane, the degree of crystallinity is at least 15%.
Preferably, it is set within a range of 20 to 80%.
本発明における酸素透過膜は、酸素透過性を利用して使
用される膜を意味するもので、このような酸素透過膜の
具体例としては、コンタクトレンズ、人工皮膚１人工角
膜、人工肺用膜、血液バッグ、溶存酸素取り込み膜（人
工えら）細胞培養床材等が挙げられる。The oxygen permeable membrane in the present invention refers to a membrane that is used by utilizing oxygen permeability, and specific examples of such oxygen permeable membranes include contact lenses, artificial skin 1, artificial cornea, and membranes for artificial lungs. , blood bags, dissolved oxygen uptake membranes (artificial gills), cell culture beds, etc.
本発明の酸素透過膜は、すぐれた酸素透過性を示すと共
に、透明性及び機械的強度にすぐれ、かつ人体に対して
も高い安全性を示す。本発明の酸素透過膜の殊にすぐれ
た利点は、含水状態においても高い酸素透過性と機械的
強度を示すことである。このような含水状態の膜は、人
体に対する親和性にすぐれ、その取扱いが容易である。The oxygen permeable membrane of the present invention exhibits excellent oxygen permeability, excellent transparency and mechanical strength, and high safety for the human body. A particularly advantageous advantage of the oxygen permeable membrane of the present invention is that it exhibits high oxygen permeability and mechanical strength even in a hydrated state. Such a membrane in a water-containing state has excellent affinity for the human body and is easy to handle.
本発明の酸素透過膜においては、その含水率は、１０〜
６０重量メ、好ましくは２０〜４０重Ｍ％に規定するの
が好ましい。In the oxygen permeable membrane of the present invention, the water content is 10 to
It is preferable to specify 60% by weight, preferably 20 to 40% by weight.
次に本発明を実施例によりさらに詳細に説明する。 Next, the present invention will be explained in more detail with reference to Examples.
実施例１
桑葉で飼育した家蚕（日１３７×支１３７）の熟蚕体内
より後部絹糸腺を取り出し、蒸留水で充分水洗した後、
絹糸腺細胞を除去した。細胞内の液状絹フィブロインを
蒸留水に分散させ、これを原材料として用いた。この水
溶液を蒸留水で約０．８％に希釈した後、これを２５℃
に設定した恒温槽内ポリエチレン膜上で蒸発乾固させて
絹フィブロイン膜を調製した。Example 1 The rear silk gland was removed from the mature silkworm body of a domestic silkworm (day 137 x branch 137) reared on mulberry leaves, and after thoroughly washing with distilled water,
The silk gland cells were removed. Intracellular liquid silk fibroin was dispersed in distilled water and used as a raw material. After diluting this aqueous solution to about 0.8% with distilled water, it was heated at 25°C.
A silk fibroin membrane was prepared by evaporation to dryness on a polyethylene membrane in a constant temperature bath set at .
得られた膜を２５℃の５０体積メメタノール水溶液中に
所定時間浸漬して処理し、その後温度２５℃で乾燥した
。このようにして得た膜の酸素透過係数を膜の含水率と
共に表−１に示す。The obtained membrane was treated by immersing it in a 50 volume memethanol aqueous solution at 25°C for a predetermined time, and then dried at a temperature of 25°C. The oxygen permeability coefficient of the membrane thus obtained is shown in Table 1 along with the moisture content of the membrane.
なお、膜の酸素透過係数の値は酸素電極法により求めた
。即ち、酸素電極の感応部に本発明の膜を装着し、所定
温度の酸素飽和水中にその電極を浸漬し、得られる電流
値から酸素の透過係数値を算出した。Note that the value of the oxygen permeability coefficient of the membrane was determined by an oxygen electrode method. That is, the membrane of the present invention was attached to the sensitive part of an oxygen electrode, the electrode was immersed in oxygen-saturated water at a predetermined temperature, and the oxygen permeability coefficient value was calculated from the obtained current value.
表−１ 指定代理人 工業技術院製品科学研究所長高橋教司Table-1 Designated Agent: Keiji Takahashi, Director, Product Science Research Institute, Agency of Industrial Science and Technology
Claims (2)
、水不溶性であることを特徴とする酸素透過膜。(1) An oxygen-permeable membrane comprising a silk fibroin membrane, containing crystalline matter and being water-insoluble.
範囲第１項の酸素透過膜。(2) The oxygen permeable membrane according to claim 1, wherein the membrane contains 10 to 60% by weight of water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62080267A JPS63246169A (en) | 1987-03-31 | 1987-03-31 | Oxygen permeable membrane |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62080267A JPS63246169A (en) | 1987-03-31 | 1987-03-31 | Oxygen permeable membrane |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63246169A true JPS63246169A (en) | 1988-10-13 |
| JPH0518616B2 JPH0518616B2 (en) | 1993-03-12 |
Family
ID=13713524
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62080267A Granted JPS63246169A (en) | 1987-03-31 | 1987-03-31 | Oxygen permeable membrane |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63246169A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05313105A (en) * | 1992-04-08 | 1993-11-26 | Agency Of Ind Science & Technol | Contact lens and its production |
-
1987
- 1987-03-31 JP JP62080267A patent/JPS63246169A/en active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05313105A (en) * | 1992-04-08 | 1993-11-26 | Agency Of Ind Science & Technol | Contact lens and its production |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0518616B2 (en) | 1993-03-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |