KR970010785A - 히루딘- 함유 배양 브로쓰에서 카복시펩티다아제 y를 불활성화시키는 방법 - Google Patents

히루딘- 함유 배양 브로쓰에서 카복시펩티다아제 y를 불활성화시키는 방법 Download PDF

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KR970010785A
KR970010785A KR1019960033769A KR19960033769A KR970010785A KR 970010785 A KR970010785 A KR 970010785A KR 1019960033769 A KR1019960033769 A KR 1019960033769A KR 19960033769 A KR19960033769 A KR 19960033769A KR 970010785 A KR970010785 A KR 970010785A
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South Korea
Prior art keywords
culture broth
hirudin
containing culture
carboxypeptidase
inactivating
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KR1019960033769A
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KR100458338B1 (ko
Inventor
이주경
신준섭
채준표
라영준
정영수
박종기
남인식
페터 크라우제
파울 하베르만
외르그 뮐러
볼프강 울머
Original Assignee
김종인
영진약품공업 주식회사
아울미히, 루츠
훽스트 아크티엔게젤샤프트
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Analytical Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Cultivation Of Plants (AREA)

Abstract

본 발명은 배양 브로쓰를 80 내지 100℃, 바람직하게는 85 내지 95℃, 특히 바람직하게는 85℃의 온도까지 연속적으로 가열하고, 가열부, 보유부 및 냉각부를 갖는 관형 장치를 통해 유동시키는 것을 포함하여, 형질전환된 효모를 발효시킴으로써 생산되는 히루딘-함유 배양 브로쓰에서 카복시펩티다아제 Y를 불활성화시키는 방법에 관한 것이다.

Description

히루딘-함유 배양 브로쓰에서 카복시펩티다아제 Y를 불활성화시키는 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (8)

  1. 배양 브로쓰를 80 내지 100℃로 연속적으로 가열하고, 가열부, 보유부 및 냉각부를 갖는 관형 장치를 통해 유동시킴을 특징으로 하여, 형질전환된 효모를 발효시킴으로써 생산되는 히루딘-함유 배양 브로쓰에서 카복시펩티다아제 Y를 불활성화시키는 방법
  2. 제1항에 있어서, 온도가 85 내지 95℃인 방법.
  3. 제2항에 있어서, 온도가 85℃인 방법.
  4. 제1항 내지 제3항 중의 어느 한 항에 있어서, 장치의 가열부에서 배양 브로쓰의 평균 잔류 시간이 40 내지 60초인 방법.
  5. 제1항 내지 제4항 중의 어느 한 항에 있어서, 장치의 가열부가 내부 표면이 기계적으로 연속 세척되는 병류 열교환기를 포함하는 방법.
  6. 제1항 내지 제5항 중의 어느 한 항에 있어서, 형질전환된 효모가 사카로마이세스 세레비시에(Saccharomyces cerevisiae)인 방법.
  7. 제1항 내지 제6항 중의 어느 한 항에 있어서, 배양 브로쓰의 pH가 6.2 내지 6.5인 방법.
  8. 제1항에 있어서, 배양 브로쓰가 극초단파의 작용에 의해 가열되는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019960033769A 1995-08-16 1996-08-14 히루딘-함유 배양브로쓰에서 카복시펩티다아제y를 불활성화하는 방법 Expired - Fee Related KR100458338B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19529997.3 1995-08-16
DE19529997A DE19529997C1 (de) 1995-08-16 1995-08-16 Verfahren zur Inaktivierung von Carboxypeptidase Y in hirudinhaltigen Kulturbrühen

Publications (2)

Publication Number Publication Date
KR970010785A true KR970010785A (ko) 1997-03-27
KR100458338B1 KR100458338B1 (ko) 2005-01-27

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ID=7769532

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KR1019960033769A Expired - Fee Related KR100458338B1 (ko) 1995-08-16 1996-08-14 히루딘-함유 배양브로쓰에서 카복시펩티다아제y를 불활성화하는 방법

Country Status (10)

Country Link
US (1) US5866399A (ko)
EP (1) EP0758655B1 (ko)
JP (1) JP3233582B2 (ko)
KR (1) KR100458338B1 (ko)
AT (1) ATE231166T1 (ko)
AU (1) AU705341B2 (ko)
CA (1) CA2183406C (ko)
DE (2) DE19529997C1 (ko)
DK (1) DK0758655T3 (ko)
ES (1) ES2189845T3 (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120258519A1 (en) * 2011-04-10 2012-10-11 Therapeutic Proteins Inc. Protein Harvesting

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4256590A (en) * 1977-05-09 1981-03-17 Exxon Research & Engineering Co. Modified heteropolysaccharides and their preparation
US4299848A (en) * 1979-06-08 1981-11-10 International Telephone And Telegraph Corporation Modified enzyme system to inhibit bread firming method for preparing same and use of same in bread and other bakery products
US4455290A (en) * 1981-04-02 1984-06-19 Research Corporation Inhibition of fibrin polymerization by a peptide isolated from fibrin Fragment D1
US4632780A (en) * 1981-07-10 1986-12-30 Seidah Nabil G N-terminal fragment of human pro-opiomelanocortin and process therefor
IT1157340B (it) 1982-11-29 1987-02-11 F I M A Dispositivo per collegare amovibilamente utensili abrasivi all'albero di levigatrici e simili
WO1985004418A1 (fr) 1984-03-27 1985-10-10 Transgene S.A. Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
DE3438296A1 (de) 1984-04-18 1985-11-07 Hoechst Ag, 6230 Frankfurt Neue polypeptide mit blutgerinnungshemmender wirkung, verfahren zu deren herstellung bzw. gewinnung, deren verwendung und diese enthaltende mittel
ATE64956T1 (de) 1984-06-14 1991-07-15 Ciba Geigy Ag Verfahren zur herstellung von thrombininhibitoren.
DE3429430A1 (de) 1984-08-10 1986-02-20 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
DE3445517C2 (de) 1984-12-13 1993-11-18 Ciba Geigy Für ein Hirudin-ähnliches Protein codierende DNA-Sequenz und Verfahren zur Herstellung eines Hirudin-ähnlichen Proteins
DE3506992A1 (de) 1985-02-27 1986-08-28 Plantorgan Werk Heinrich G.E. Christensen, KG, 2903 Bad Zwischenahn Modifizierte hirudine, verfahren zu deren herstellung und pharmazeutische mittel, die diese wirkstoffe enthalten
ES2055149T3 (es) 1985-04-11 1994-08-16 Hoechst Ag Derivado de hirudina.
FR2593518B1 (fr) 1985-05-02 1989-09-08 Transgene Sa Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
EP0209061B1 (de) 1985-07-17 1994-01-12 Hoechst Aktiengesellschaft Neue Polypeptide mit blutgerinnungshemmender Wirkung, Verfahren zu deren Herstellung bzw. Gewinnung, deren Verwendung und diese enthaltende Mittel
AU614121B2 (en) 1988-05-04 1991-08-22 Novartis Ag Improvements in the production of polypeptides
FR2645175B1 (fr) 1989-03-31 1994-02-18 Transgene Sa Souche de saccharomyces cerevisiaeproductrice d'une proteine heterologue et procede de preparation de ladite proteine heterologue par fermentation de ladite souche
JP2543235B2 (ja) * 1990-06-29 1996-10-16 松下電器産業株式会社 Ｉｃカ―ドアダプタ
GB2249096A (en) 1990-10-11 1992-04-29 Ciba Geigy Saccharomyces cerevisiae strains lacking carboxypeptidase yscY activity for expression of proteins at high yields
CA2100618A1 (en) * 1992-07-29 1994-01-30 Janice L. Bryson Egg pasteurization

Also Published As

Publication number Publication date
AU6206196A (en) 1997-03-06
AU705341B2 (en) 1999-05-20
EP0758655A1 (de) 1997-02-19
DE59610052D1 (de) 2003-02-20
ES2189845T3 (es) 2003-07-16
ATE231166T1 (de) 2003-02-15
JP3233582B2 (ja) 2001-11-26
JPH09121854A (ja) 1997-05-13
DE19529997C1 (de) 1997-04-10
CA2183406C (en) 2008-07-08
CA2183406A1 (en) 1997-02-17
EP0758655B1 (de) 2003-01-15
HK1012013A1 (en) 1999-07-23
US5866399A (en) 1999-02-02
KR100458338B1 (ko) 2005-01-27
DK0758655T3 (da) 2003-04-14

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