PL2748179T3 - Oczyszczanie białka metodą anionowej chromatografii jonowymiennej - Google Patents

Oczyszczanie białka metodą anionowej chromatografii jonowymiennej

Info

Publication number
PL2748179T3
PL2748179T3 PL12770496T PL12770496T PL2748179T3 PL 2748179 T3 PL2748179 T3 PL 2748179T3 PL 12770496 T PL12770496 T PL 12770496T PL 12770496 T PL12770496 T PL 12770496T PL 2748179 T3 PL2748179 T3 PL 2748179T3
Authority
PL
Poland
Prior art keywords
anion exchange
exchange chromatography
protein purification
purification
protein
Prior art date
Application number
PL12770496T
Other languages
English (en)
Inventor
Artur Mitterer
Meinhard Hasslacher
Christian Fiedler
Original Assignee
Takeda Pharmaceutical Company Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takeda Pharmaceutical Company Limited filed Critical Takeda Pharmaceutical Company Limited
Publication of PL2748179T3 publication Critical patent/PL2748179T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Diabetes (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
PL12770496T 2011-10-14 2012-10-12 Oczyszczanie białka metodą anionowej chromatografii jonowymiennej PL2748179T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161547579P 2011-10-14 2011-10-14
EP12770496.3A EP2748179B1 (en) 2011-10-14 2012-10-12 Protein purification by anion exchange chromatography
PCT/EP2012/070257 WO2013053887A1 (en) 2011-10-14 2012-10-12 Protein purification by anion exchange chromatography

Publications (1)

Publication Number Publication Date
PL2748179T3 true PL2748179T3 (pl) 2022-04-11

Family

ID=47010608

Family Applications (1)

Application Number Title Priority Date Filing Date
PL12770496T PL2748179T3 (pl) 2011-10-14 2012-10-12 Oczyszczanie białka metodą anionowej chromatografii jonowymiennej

Country Status (8)

Country Link
US (1) US10378004B2 (pl)
EP (1) EP2748179B1 (pl)
JP (2) JP6236008B2 (pl)
AU (1) AU2012322948B2 (pl)
ES (1) ES2908920T3 (pl)
LT (1) LT2748179T (pl)
PL (1) PL2748179T3 (pl)
WO (1) WO2013053887A1 (pl)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103025757A (zh) 2010-04-29 2013-04-03 巴克斯特国际有限公司 二价阳离子结合蛋白在阴离子交换树脂上的纯化方法
WO2013053888A1 (en) 2011-10-14 2013-04-18 Baxter International Inc. Protein purification by anion exchange chromatography
PL2748179T3 (pl) 2011-10-14 2022-04-11 Takeda Pharmaceutical Company Limited Oczyszczanie białka metodą anionowej chromatografii jonowymiennej
TR201808584T4 (tr) 2013-03-15 2018-07-23 Baxalta GmbH Anyon değişimi kromotografisi aracılığıyla k vitaminine bağlı proteinler için saflaştırma yöntemi.
AR102331A1 (es) * 2014-08-12 2017-02-22 Baxalta Inc Activación del factor x
ES2906118T3 (es) 2016-12-22 2022-04-13 Km Biologics Co Ltd Método cromatográfico para recolectar factor VII de coagulación sanguínea con alto rendimiento
AU2021210905A1 (en) * 2020-01-24 2022-09-01 Mural Oncology, Inc. Methods of purification
JP2022023813A (ja) * 2020-07-27 2022-02-08 ファイザー・インク 組換え生産されたｒｓｖタンパク質の精製方法における陰イオン交換クロマトグラフィー用洗浄溶液の改良
WO2022172284A1 (en) * 2021-02-11 2022-08-18 Gennova Biopharmaceuticals Ltd. Purification of a therapeutic protein
TW202500572A (zh) * 2023-03-14 2025-01-01 美商安進公司 使用一級胺配體的陰離子交換層析製程

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2045167T5 (es) 1987-10-23 1996-07-01 Centre Regional De Transfusion Preparacion de concentrado de factor ix humano de elevada pureza y de otras proteinas plasmaticas.
US4981952A (en) * 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
DK0658168T3 (da) 1992-08-27 2001-01-22 Sanquin Bloedvoorziening Antistoffer, som er specifikke for et hæmostatisk protein, anvendelse heraf til isolering af intakt protein, hæmostatiske p
DE4406515C1 (de) * 1994-02-28 1995-10-19 Immuno Ag Verfahren zur Isolierung und Reinigung Vitamin K-abhängiger Proteine
US5714583A (en) * 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods
DE69841459D1 (en) * 1997-02-14 2010-03-11 American Nat Red Cross Expression des active human factor ix im brustdrüsengewebe transgener tiere
CN1250567C (zh) 2000-07-21 2006-04-12 财团法人化学及血清疗法研究所 钙离子结合蛋白的提纯方法
US20040106779A1 (en) 2002-12-03 2004-06-03 Bigler Douglas E. Modified factor IX preparation
CN102718859A (zh) * 2004-09-29 2012-10-10 诺和诺德医疗保健公司 通过分级洗脱从阴离子交换材料中纯化批量凝血因子vii多肽
EP1831242B1 (en) * 2004-12-23 2012-09-26 Novo Nordisk Health Care AG Reduction of the content of protein contaminants in compositions comprising a vitamin k-dependent protein of interest
EP1924688A1 (en) 2005-09-01 2008-05-28 Novo Nordisk Health Care AG Purification of coagulation factor vii polypeptides
WO2007101681A1 (en) 2006-03-07 2007-09-13 Baxter International Inc Highly phosphorylated and sulfated recombinant factor ix
FR2901796A1 (fr) 2006-05-31 2007-12-07 Lab Francais Du Fractionnement Procede d'extraction d'une ou de plusieurs proteines presentes dans du lait
FR2901707B1 (fr) 2006-05-31 2017-09-29 Lab Francais Du Fractionnement Composition de facteur vii recombinant ou transgenique, chaque molecule de facteur vii possedant deux sites de n-glycosylation a motifs glycanniques definis
US8399632B2 (en) * 2007-02-28 2013-03-19 Baxter International Inc. Method for the purification of recombinant blood coagulation factor IX enriched in sulfated and/or phosphorylated molecules
EP4328305A3 (en) 2009-12-18 2024-08-14 CSL Ltd. Method of purifying polypeptides
CN103025757A (zh) * 2010-04-29 2013-04-03 巴克斯特国际有限公司 二价阳离子结合蛋白在阴离子交换树脂上的纯化方法
WO2013053888A1 (en) 2011-10-14 2013-04-18 Baxter International Inc. Protein purification by anion exchange chromatography
PL2748179T3 (pl) 2011-10-14 2022-04-11 Takeda Pharmaceutical Company Limited Oczyszczanie białka metodą anionowej chromatografii jonowymiennej

Also Published As

Publication number Publication date
US10378004B2 (en) 2019-08-13
AU2012322948A1 (en) 2013-05-16
WO2013053887A1 (en) 2013-04-18
JP6236008B2 (ja) 2017-11-22
ES2908920T3 (es) 2022-05-04
JP6820232B2 (ja) 2021-01-27
LT2748179T (lt) 2022-04-11
EP2748179A1 (en) 2014-07-02
AU2012322948B2 (en) 2014-11-06
EP2748179B1 (en) 2021-12-29
JP2014530230A (ja) 2014-11-17
US20140302591A1 (en) 2014-10-09
HK1198588A1 (en) 2015-04-30
JP2017160248A (ja) 2017-09-14

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