PL2970376T3 - Sposób oczyszczania białek zależnych od witaminy k za pomocą chromatografii anionowymiennej - Google Patents

Sposób oczyszczania białek zależnych od witaminy k za pomocą chromatografii anionowymiennej

Info

Publication number
PL2970376T3
PL2970376T3 PL14709972T PL14709972T PL2970376T3 PL 2970376 T3 PL2970376 T3 PL 2970376T3 PL 14709972 T PL14709972 T PL 14709972T PL 14709972 T PL14709972 T PL 14709972T PL 2970376 T3 PL2970376 T3 PL 2970376T3
Authority
PL
Poland
Prior art keywords
vitamin
anion exchange
exchange chromatography
purification method
dependent proteins
Prior art date
Application number
PL14709972T
Other languages
English (en)
Inventor
Artur Mitterer
Meinhard Hasslacher
Christian Fiedler
Dominik MITTERGRADNEGGER
Original Assignee
Baxalta Incorporated
Baxalta GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxalta Incorporated, Baxalta GmbH filed Critical Baxalta Incorporated
Publication of PL2970376T3 publication Critical patent/PL2970376T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Diabetes (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
PL14709972T 2013-03-15 2014-03-14 Sposób oczyszczania białek zależnych od witaminy k za pomocą chromatografii anionowymiennej PL2970376T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361792772P 2013-03-15 2013-03-15
PCT/EP2014/055120 WO2014140289A1 (en) 2013-03-15 2014-03-14 Purification method for vitamin k dependent proteins by anion exchange chromatography
EP14709972.5A EP2970376B1 (en) 2013-03-15 2014-03-14 Purification method for vitamin k dependent proteins by anion exchange chromatography

Publications (1)

Publication Number Publication Date
PL2970376T3 true PL2970376T3 (pl) 2018-10-31

Family

ID=50277247

Family Applications (1)

Application Number Title Priority Date Filing Date
PL14709972T PL2970376T3 (pl) 2013-03-15 2014-03-14 Sposób oczyszczania białek zależnych od witaminy k za pomocą chromatografii anionowymiennej

Country Status (12)

Country Link
US (1) US10202417B2 (pl)
EP (1) EP2970376B1 (pl)
JP (1) JP6571011B2 (pl)
AU (1) AU2014230101B2 (pl)
DK (1) DK2970376T3 (pl)
ES (1) ES2682928T3 (pl)
HU (1) HUE037677T2 (pl)
NZ (1) NZ629845A (pl)
PL (1) PL2970376T3 (pl)
SI (1) SI2970376T1 (pl)
TR (1) TR201808584T4 (pl)
WO (1) WO2014140289A1 (pl)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR102331A1 (es) 2014-08-12 2017-02-22 Baxalta Inc Activación del factor x

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4981952A (en) 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
CA2143125C (en) 1992-08-27 2008-09-23 Koenraad Mertens Antibodies specific for a haemostatic protein, their use for isolating intact protein, haemostatic compositions devoid of proteolytic cleavage products of the protein
DE4406515C1 (de) * 1994-02-28 1995-10-19 Immuno Ag Verfahren zur Isolierung und Reinigung Vitamin K-abhängiger Proteine
US5714583A (en) 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods
US6344596B1 (en) 1997-02-14 2002-02-05 American Red Cross Expression of active human factor IX in mammary tissue and of milk non human transgenic mammals
KR100841599B1 (ko) 2000-07-21 2008-06-26 자이단호진 가가쿠오요비겟세이료호겐쿠쇼 칼슘 이온-결합 단백질의 정제 방법
US20090043080A1 (en) * 2004-09-29 2009-02-12 Novo Nordisk Healthcare A/G Purification of a Bulk of a Factor VII Polypeptide by Fractionated Elution from an Anion-Exchange Material
EP2360171A1 (en) 2004-12-23 2011-08-24 Novo Nordisk Health Care AG Reduction of the content of protein contaminants in compositions comprising a vitamin K-dependent protein of interest
EP1924688A1 (en) 2005-09-01 2008-05-28 Novo Nordisk Health Care AG Purification of coagulation factor vii polypeptides
FR2901796A1 (fr) 2006-05-31 2007-12-07 Lab Francais Du Fractionnement Procede d'extraction d'une ou de plusieurs proteines presentes dans du lait
FR2901707B1 (fr) 2006-05-31 2017-09-29 Lab Francais Du Fractionnement Composition de facteur vii recombinant ou transgenique, chaque molecule de facteur vii possedant deux sites de n-glycosylation a motifs glycanniques definis
DK2125866T3 (da) * 2007-02-28 2013-07-29 Baxter Int Fremgangsmåde til rensning af rekombinant blodkoagulationsfaktor IX, som er beriget med sulfaterede og/eller phosphorylerede molekyler
WO2011073235A1 (en) 2009-12-18 2011-06-23 Csl Ltd. Method of purifying polypeptides
SG185005A1 (en) * 2010-04-29 2012-11-29 Baxter Int Purification method for divalent cation binding proteins on anion exchange resin
ES2700933T3 (es) * 2011-10-14 2019-02-20 Baxalta GmbH Purificación de proteínas mediante cromatografía de intercambio aniónico
AU2012322948B2 (en) * 2011-10-14 2014-11-06 Takeda Pharmaceutical Company Limited Protein purification by anion exchange chromatography

Also Published As

Publication number Publication date
EP2970376B1 (en) 2018-05-23
US20160039869A1 (en) 2016-02-11
DK2970376T3 (en) 2018-07-02
JP2016512215A (ja) 2016-04-25
WO2014140289A1 (en) 2014-09-18
EP2970376A1 (en) 2016-01-20
AU2014230101A1 (en) 2015-10-08
SI2970376T1 (sl) 2018-09-28
US10202417B2 (en) 2019-02-12
TR201808584T4 (tr) 2018-07-23
NZ629845A (en) 2016-10-28
ES2682928T3 (es) 2018-09-24
HK1219285A1 (en) 2017-03-31
AU2014230101B2 (en) 2018-04-19
JP6571011B2 (ja) 2019-09-04
HUE037677T2 (hu) 2018-09-28

Similar Documents

Publication Publication Date Title
EP3041593A4 (en) Method for chromatography reuse
PT3004163T (pt) Método para purificação de anticorpo
IL241948B (en) Method for gaze tracking
IL288023A (en) A method for isolating an antibody with a low isoelectric point
SG11201602061YA (en) Novel antibody purification method and antibody obtained therefrom, and novel antibody purification method using cation exchanger and antibody obtained therefrom
SG11201505204VA (en) Protein purification process
IL240753A0 (en) Antibody purification methods
EP3067367A4 (en) Method for purifying recombinant protein
LT2748179T (lt) Baltymų gryninimas anijonų mainų chromatografija
SG11201601994VA (en) Method for preparing zeolite ssz-35
PL2891626T3 (pl) Sposób oczyszczania difluorofosforanu
HUE048326T2 (hu) Béta-hidroxi-béta-metil-vajsav tisztítási módszer
EP3023403A4 (en) Method for purifying 2-fluorobutane
EP3015473A4 (en) Method for purifying oligosaccharide peptide
SG11201601819WA (en) Process for separating methacrolein
IL241779A0 (en) Method for protein production
EP3059585A4 (en) Method for manufacturing small-diameter column for chromatography
EP3063304B8 (en) Method for finishing flow elements
SG11201604277QA (en) Method for producing instant noodles
SG11201509776VA (en) Method for producing acrolein
GB201301859D0 (en) Regeneration of an ion exchange column
IL243038A0 (en) A method for the preparation of pyrrolidine-2-carboxylic acid derivatives
SG11201601934YA (en) Method for preparing zeolite ssz-35
HUE051329T2 (hu) Eljárás klór tisztítására frakcionálással
SI2970376T1 (sl) Metoda čiščenja za vitamin k odvisne proteine z anionsko izmenjevalno kromatografijo