LT3474669T - Barkodu pažymėtų kombinatorinių bibliotekų generavimo būdai - Google Patents
Barkodu pažymėtų kombinatorinių bibliotekų generavimo būdaiInfo
- Publication number
- LT3474669T LT3474669T LTEPPCT/US2017/039146T LTUS2017039146T LT3474669T LT 3474669 T LT3474669 T LT 3474669T LT US2017039146 T LTUS2017039146 T LT US2017039146T LT 3474669 T LT3474669 T LT 3474669T
- Authority
- LT
- Lithuania
- Prior art keywords
- barcode
- generation
- methods
- combination libraries
- labeled combination
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662354516P | 2016-06-24 | 2016-06-24 | |
| US201662367386P | 2016-07-27 | 2016-07-27 | |
| US201762483930P | 2017-04-10 | 2017-04-10 | |
| PCT/US2017/039146 WO2017223538A1 (en) | 2016-06-24 | 2017-06-23 | Methods for generating barcoded combinatorial libraries |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| LT3474669T true LT3474669T (lt) | 2022-06-10 |
Family
ID=60676029
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LTEPPCT/US2017/039146T LT3474669T (lt) | 2016-06-24 | 2017-06-23 | Barkodu pažymėtų kombinatorinių bibliotekų generavimo būdai |
Country Status (10)
| Country | Link |
|---|---|
| US (5) | US10017760B2 (lt) |
| EP (1) | EP3474669B1 (lt) |
| JP (1) | JP2019518478A (lt) |
| CN (1) | CN109688820B (lt) |
| AU (1) | AU2017280353B2 (lt) |
| CA (1) | CA3029254A1 (lt) |
| DK (1) | DK3474669T3 (lt) |
| ES (1) | ES2915562T3 (lt) |
| LT (1) | LT3474669T (lt) |
| WO (1) | WO2017223538A1 (lt) |
Families Citing this family (106)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4063503A1 (en) | 2014-02-11 | 2022-09-28 | The Regents of the University of Colorado, a body corporate | Crispr enabled multiplexed genome engineering |
| CA2994160C (en) | 2015-07-30 | 2021-08-10 | Massachusetts Eye And Ear Infirmary | Ancestral virus sequences and uses thereof |
| US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
| US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
| JP6821598B2 (ja) | 2015-12-07 | 2021-01-27 | ザイマージェン インコーポレイテッド | Ｃｏｒｙｎｅｂａｃｔｅｒｉｕｍ ｇｌｕｔａｍｉｃｕｍ由来のプロモーター |
| EP3458579B1 (en) * | 2016-05-20 | 2023-07-05 | Braingene AB | Destabilising domains for conditionally stabilising a protein |
| US10337051B2 (en) | 2016-06-16 | 2019-07-02 | The Regents Of The University Of California | Methods and compositions for detecting a target RNA |
| US11293021B1 (en) | 2016-06-23 | 2022-04-05 | Inscripta, Inc. | Automated cell processing methods, modules, instruments, and systems |
| US10017760B2 (en) | 2016-06-24 | 2018-07-10 | Inscripta, Inc. | Methods for generating barcoded combinatorial libraries |
| EP3478833A4 (en) | 2016-06-30 | 2019-10-02 | Zymergen, Inc. | METHOD FOR PRODUCING A BACTERIAL HEMOGLOBIN LIBRARY AND ITS USE |
| JP2019519241A (ja) | 2016-06-30 | 2019-07-11 | ザイマージェン インコーポレイテッド | グルコース透過酵素ライブラリーを生成するための方法およびその使用 |
| US20180004537A1 (en) * | 2016-07-01 | 2018-01-04 | Microsoft Technology Licensing, Llc | Molecular State Machines |
| US11359234B2 (en) | 2016-07-01 | 2022-06-14 | Microsoft Technology Licensing, Llc | Barcoding sequences for identification of gene expression |
| US10892034B2 (en) | 2016-07-01 | 2021-01-12 | Microsoft Technology Licensing, Llc | Use of homology direct repair to record timing of a molecular event |
| CN109477130B (zh) | 2016-07-01 | 2022-08-30 | 微软技术许可有限责任公司 | 通过迭代dna编辑的存储 |
| CA3034110A1 (en) * | 2016-08-17 | 2018-02-22 | Katholieke Universiteit Leuven | Drug-target identification by rapid selection of drug resistance mutations |
| CA3038960A1 (en) * | 2016-09-30 | 2018-04-05 | The Regents Of The University Of California | Rna-guided nucleic acid modifying enzymes and methods of use thereof |
| JP7306696B2 (ja) | 2016-09-30 | 2023-07-11 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | Ｒｎａ誘導型核酸修飾酵素及びその使用方法 |
| US10011849B1 (en) | 2017-06-23 | 2018-07-03 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| JP7136816B2 (ja) * | 2017-06-23 | 2022-09-13 | インスクリプタ， インコーポレイテッド | 核酸誘導型ヌクレアーゼ |
| US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| MX2019015505A (es) | 2017-06-30 | 2020-07-28 | Inscripta Inc | Metodos, modulos, instrumentos y sistemas de procesamiento de celulas automatizados. |
| US10738327B2 (en) | 2017-08-28 | 2020-08-11 | Inscripta, Inc. | Electroporation cuvettes for automation |
| CA3075532A1 (en) | 2017-09-15 | 2019-03-21 | The Board Of Trustees Of The Leland Stanford Junior University | Multiplex production and barcoding of genetically engineered cells |
| EP3687621A4 (en) | 2017-09-30 | 2021-08-11 | Inscripta, Inc. | Flow through electroporation instrumentation |
| WO2019089808A1 (en) | 2017-11-01 | 2019-05-09 | The Regents Of The University Of California | Class 2 crispr/cas compositions and methods of use |
| JP2021503278A (ja) | 2017-11-01 | 2021-02-12 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | ＣａｓＺ組成物及び使用方法 |
| WO2019089804A1 (en) | 2017-11-01 | 2019-05-09 | The Regents Of The University Of California | Casy compositions and methods of use |
| US12145962B2 (en) * | 2018-02-05 | 2024-11-19 | The Regents Of The University Of Colorado, A Body Corporate | Construction and methods of use of a barcoded and gene edited DNA library |
| CN111902536B (zh) * | 2018-02-15 | 2024-07-30 | 西格马-奥尔德里奇有限责任公司 | 用于真核基因组修饰的改造的cas9系统 |
| WO2019190874A1 (en) | 2018-03-29 | 2019-10-03 | Inscripta, Inc. | Automated control of cell growth rates for induction and transformation |
| EP3775266A4 (en) | 2018-04-05 | 2021-06-30 | Massachusetts Eye and Ear Infirmary | METHOD OF MANUFACTURING AND USING COMBINATIONAL BAR-CODED NUCLEIC ACID LIBRARIES WITH DEFINED VARIATION |
| US10376889B1 (en) | 2018-04-13 | 2019-08-13 | Inscripta, Inc. | Automated cell processing instruments comprising reagent cartridges |
| US10508273B2 (en) | 2018-04-24 | 2019-12-17 | Inscripta, Inc. | Methods for identifying selective binding pairs |
| US10526598B2 (en) | 2018-04-24 | 2020-01-07 | Inscripta, Inc. | Methods for identifying T-cell receptor antigens |
| US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
| AU2019292919A1 (en) | 2018-06-30 | 2021-03-11 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| EP3821008A1 (en) * | 2018-07-12 | 2021-05-19 | Keygene N.V. | Type v crispr/nuclease-system for genome editing in plant cells |
| EP3830301B1 (en) | 2018-08-01 | 2024-05-22 | Mammoth Biosciences, Inc. | Programmable nuclease compositions and methods of use thereof |
| US11142740B2 (en) | 2018-08-14 | 2021-10-12 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| US10752874B2 (en) | 2018-08-14 | 2020-08-25 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US10532324B1 (en) | 2018-08-14 | 2020-01-14 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US12275964B2 (en) | 2018-08-22 | 2025-04-15 | The Regents Of The University Of California | Variant type V CRISPR/Cas effector polypeptides and methods of use thereof |
| US11718847B2 (en) * | 2018-08-29 | 2023-08-08 | Agilent Technologies, Inc. | Amplifying oligonucleotides and producing libraries of dual guide constructs |
| US11965154B2 (en) | 2018-08-30 | 2024-04-23 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| EP3861112A4 (en) * | 2018-10-04 | 2022-09-21 | The Regents of the University of Colorado, A Body Corporate | ENGINEERED CHIMERIC NUCLEIC ACID-GUIDED NUCLEAR CONSTRUCTS AND THEIR USES |
| AU2019368215B2 (en) | 2018-10-22 | 2023-05-18 | Inscripta, Inc. | Engineered enzymes |
| US11214781B2 (en) | 2018-10-22 | 2022-01-04 | Inscripta, Inc. | Engineered enzyme |
| CA3118440A1 (en) * | 2018-11-03 | 2020-05-07 | Blueallele, Llc | Methods for comparing efficacy of donor molecules |
| EP3931313A2 (en) | 2019-01-04 | 2022-01-05 | Mammoth Biosciences, Inc. | Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection |
| EP3921411A4 (en) * | 2019-02-08 | 2023-03-08 | The Board of Trustees of the Leland Stanford Junior University | MANUFACTURE AND TRACKING OF GM CELLS WITH COMBINATORY GENETIC MODIFICATIONS |
| US11001831B2 (en) | 2019-03-25 | 2021-05-11 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
| CN113631713A (zh) | 2019-03-25 | 2021-11-09 | 因思科瑞普特公司 | 酵母中的同时多重基因组编辑 |
| JP2022530029A (ja) * | 2019-04-24 | 2022-06-27 | スポットライト セラピューティクス | 核酸ガイドヌクレアーゼ細胞ターゲティングスクリーニングのための方法及び組成物 |
| AU2020288623A1 (en) | 2019-06-06 | 2022-01-06 | Inscripta, Inc. | Curing for recursive nucleic acid-guided cell editing |
| PH12021553076A1 (en) | 2019-06-07 | 2023-08-14 | Scribe Therapeutics Inc | Engineered casx systems |
| AU2020291467A1 (en) * | 2019-06-14 | 2021-12-23 | Arbor Biotechnologies, Inc. | Novel crispr DNA targeting enzymes and systems |
| US10907125B2 (en) | 2019-06-20 | 2021-02-02 | Inscripta, Inc. | Flow through electroporation modules and instrumentation |
| US10920189B2 (en) | 2019-06-21 | 2021-02-16 | Inscripta, Inc. | Genome-wide rationally-designed mutations leading to enhanced lysine production in E. coli |
| US10927385B2 (en) | 2019-06-25 | 2021-02-23 | Inscripta, Inc. | Increased nucleic-acid guided cell editing in yeast |
| US20210010006A1 (en) * | 2019-07-08 | 2021-01-14 | Inscripta, Inc. | Increased nucleic acid-guided cell editing via a lexa-rad51 fusion protein |
| US20220348925A1 (en) | 2019-09-09 | 2022-11-03 | Scribe Therapeutics Inc. | Compositions and methods for the targeting of sod1 |
| CA3153700A1 (en) | 2019-09-09 | 2021-03-18 | Scribe Therapeutics Inc. | Compositions and methods for use in immunotherapy |
| US20220403553A1 (en) * | 2019-09-12 | 2022-12-22 | Glaxosmithkline Intellectual Property Development Limited | Method for screening libraries |
| EP4041879A1 (en) * | 2019-10-10 | 2022-08-17 | Inscripta, Inc. | Split crispr nuclease tethering system |
| CN115052980B (zh) * | 2019-11-18 | 2024-09-10 | 苏州齐禾生科生物科技有限公司 | 衍生自黄杆菌的基因编辑系统 |
| US11203762B2 (en) | 2019-11-19 | 2021-12-21 | Inscripta, Inc. | Methods for increasing observed editing in bacteria |
| CA3159316A1 (en) | 2019-12-06 | 2021-06-10 | Benjamin OAKES | Compositions and methods for the targeting of rhodopsin |
| KR102776469B1 (ko) | 2019-12-10 | 2025-03-05 | 인스크립타 인코포레이티드 | 신규 mad 뉴클레아제 |
| US10704033B1 (en) * | 2019-12-13 | 2020-07-07 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| EP4077727A1 (en) * | 2019-12-18 | 2022-10-26 | Basf Se | Nucleic acid analysis methods and apparatus |
| EP4069851A4 (en) | 2019-12-18 | 2023-11-22 | Inscripta, Inc. | Cascade/dcas3 complementation assays for in vivo detection of nucleic acid-guided nuclease edited cells |
| EP4081641A4 (en) * | 2019-12-24 | 2023-12-27 | Asklepios Biopharmaceutical, Inc. | Method for identifying regulatory elements conformationally |
| US10689669B1 (en) | 2020-01-11 | 2020-06-23 | Inscripta, Inc. | Automated multi-module cell processing methods, instruments, and systems |
| EP4087923A1 (en) * | 2020-01-11 | 2022-11-16 | Inscripta, Inc. | Cell populations with rationally designed edits |
| AU2021213705A1 (en) | 2020-01-27 | 2022-06-16 | Inscripta, Inc. | Electroporation modules and instrumentation |
| US20210317444A1 (en) * | 2020-04-08 | 2021-10-14 | Inscripta, Inc. | System and method for gene editing cassette design |
| WO2021211972A1 (en) * | 2020-04-16 | 2021-10-21 | Zymergen Inc. | Circular-permuted nucleic acids for homology-directed editing |
| US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
| US11787841B2 (en) | 2020-05-19 | 2023-10-17 | Inscripta, Inc. | Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli |
| US20230183749A1 (en) * | 2020-05-19 | 2023-06-15 | Board Of Regents, The University Of Texas System | Genetic physical unclonable functions and methods of use thereof |
| WO2021247942A2 (en) * | 2020-06-04 | 2021-12-09 | Inscripta, Inc. | Methods and compositions for crispr editing of cells and correlating the edits to a resulting cellular nucleic acid profile |
| JP7419168B2 (ja) * | 2020-06-10 | 2024-01-22 | 株式会社東芝 | 改変型ｐｉｇｇｙＢａｃトランスポゼースのポリペプチド、それをコードするポリヌクレオチド、導入キャリア、キット、細胞のゲノムに目的配列を組込む方法及び細胞製造方法 |
| EP4165180A4 (en) * | 2020-06-16 | 2024-10-23 | Bio-Techne Corporation | MODIFIED MAD7-DIRECTED ENDONUCLEASE |
| WO2022060749A1 (en) * | 2020-09-15 | 2022-03-24 | Inscripta, Inc. | Crispr editing to embed nucleic acid landing pads into genomes of live cells |
| WO2022072878A1 (en) * | 2020-10-02 | 2022-04-07 | Inscripta, Inc. | Methods and systems for modeling of design representation in a library of editing cassettes |
| US11512297B2 (en) | 2020-11-09 | 2022-11-29 | Inscripta, Inc. | Affinity tag for recombination protein recruitment |
| AU2021392719B2 (en) | 2020-12-03 | 2025-08-14 | Scribe Therapeutics Inc. | Engineered class 2 type v crispr systems |
| AU2021415461A1 (en) * | 2021-01-04 | 2023-08-17 | Inscripta, Inc. | Mad nucleases |
| US20240376451A1 (en) | 2021-01-07 | 2024-11-14 | Inscripta, Inc. | Mad nucleases |
| US11884924B2 (en) | 2021-02-16 | 2024-01-30 | Inscripta, Inc. | Dual strand nucleic acid-guided nickase editing |
| BR112023018157A2 (pt) * | 2021-03-09 | 2023-10-31 | Illumina Inc | Análise de expressão de variantes codificantes de proteína em células |
| WO2022226085A1 (en) * | 2021-04-20 | 2022-10-27 | The Board Of Trustees Of The Leland Stanford Junior University | Compressive molecular probes for genomic editing and tracking |
| WO2022261150A2 (en) | 2021-06-09 | 2022-12-15 | Scribe Therapeutics Inc. | Particle delivery systems |
| WO2023288018A2 (en) * | 2021-07-14 | 2023-01-19 | Ultima Genomics, Inc. | Barcode selection |
| CN118511225A (zh) * | 2021-09-02 | 2024-08-16 | 华盛顿大学 | 多重时间分辨分子信号记录器及相关方法 |
| JP2024534523A (ja) | 2021-09-21 | 2024-09-20 | スクライブ・セラピューティクス・インコーポレイテッド | 操作されたｃａｓｘリプレッサー系 |
| EP4423265A1 (en) * | 2021-10-26 | 2024-09-04 | Inscripta, Inc. | Processes for measuring strain fitness and/or genotype selection in bioreactors |
| CN114022491B (zh) * | 2021-10-27 | 2022-05-10 | 安徽医科大学 | 基于改进空间金字塔模型的小数据集食管癌肿瘤靶区影像自动勾画方法 |
| WO2023137233A2 (en) * | 2022-01-17 | 2023-07-20 | Danmarks Tekniske Universitet | Compositions and methods for editing genomes |
| WO2023240076A1 (en) | 2022-06-07 | 2023-12-14 | Scribe Therapeutics Inc. | Compositions and methods for the targeting of pcsk9 |
| EP4384213A1 (en) | 2022-11-02 | 2024-06-19 | Centre For Virology, Vaccinology and Therapeutics Limited | Interferon-producing universal sarbecovirus vaccines, and uses thereof |
| US12186388B2 (en) | 2022-11-02 | 2025-01-07 | Centre For Virology, Vaccinology And Therapeutics Limited | Interferon-producing universal sarbecovirus vaccines, and uses thereof |
| CN116286898A (zh) * | 2023-04-12 | 2023-06-23 | 山东大学 | 甲硫氨酸合成酶基因HMTs及其应用 |
| WO2025038872A1 (en) * | 2023-08-15 | 2025-02-20 | Bio-Techne Corporation | Compositions and methods related to crispr-associated enzymes |
| CN119432813A (zh) * | 2023-12-29 | 2025-02-14 | 武汉艾迪晶生物科技有限公司 | Cas蛋白、组合蛋白、核酸分子、核糖核蛋白复合物、重组载体、转基因细胞及其应用 |
Family Cites Families (99)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US1001760A (en) | 1911-03-16 | 1911-08-29 | William F Mcgregor | Floating fish-trap. |
| US1001184A (en) | 1911-04-20 | 1911-08-22 | Charles M Coover | Non-slipping device. |
| US6562594B1 (en) | 1999-09-29 | 2003-05-13 | Diversa Corporation | Saturation mutagenesis in directed evolution |
| US20030044866A1 (en) | 2001-08-15 | 2003-03-06 | Charles Boone | Yeast arrays, methods of making such arrays, and methods of analyzing such arrays |
| US7547534B2 (en) | 2002-06-14 | 2009-06-16 | Verenium Corporation | Methods for making a composition to treat a wood, a pulp or a paper |
| JP4447977B2 (ja) | 2004-06-30 | 2010-04-07 | 富士通マイクロエレクトロニクス株式会社 | セキュアプロセッサ、およびセキュアプロセッサ用プログラム。 |
| ES2398918T3 (es) | 2005-08-26 | 2013-03-22 | Dupont Nutrition Biosciences Aps | Un método y una ordenación para soportar verticalmente elementos de resistencia eléctrica pendientes |
| DK2034848T3 (en) | 2006-06-16 | 2017-02-06 | Dupont Nutrition Biosci Aps | STREPTOCOCCUS THERMOPHILUS BACTERIA |
| WO2008052101A2 (en) | 2006-10-25 | 2008-05-02 | President And Fellows Of Harvard College | Multiplex automated genome engineering |
| US9309511B2 (en) | 2007-08-28 | 2016-04-12 | The Johns Hopkins University | Functional assay for identification of loss-of-function mutations in genes |
| US20140121118A1 (en) | 2010-11-23 | 2014-05-01 | Opx Biotechnologies, Inc. | Methods, systems and compositions regarding multiplex construction protein amino-acid substitutions and identification of sequence-activity relationships, to provide gene replacement such as with tagged mutant genes, such as via efficient homologous recombination |
| US20150368639A1 (en) | 2011-04-14 | 2015-12-24 | Ryan T. Gill | Compositions, methods and uses for multiplex protein sequence activity relationship mapping |
| PH12014501360B1 (en) | 2011-12-16 | 2022-05-20 | Targetgene Biotechnologies Ltd | Compositions and methods for modifying a predetermined target nucleic acid sequence |
| US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| EP3241902B1 (en) | 2012-05-25 | 2018-02-28 | The Regents of The University of California | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription |
| KR102437522B1 (ko) | 2012-05-25 | 2022-08-26 | 셀렉티스 | 면역요법을 위한 동종이형 및 면역억제제 저항성인 ｔ 세포의 조작 방법 |
| HK1207111A1 (en) | 2012-08-03 | 2016-01-22 | 加利福尼亚大学董事会 | Methods and compositions for controlling gene expression by rna processing |
| EP4357457B1 (en) | 2012-10-23 | 2024-10-16 | Toolgen Incorporated | Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof |
| EP3363902B1 (en) | 2012-12-06 | 2019-11-27 | Sigma Aldrich Co. LLC | Crispr-based genome modification and regulation |
| CN105121648B (zh) | 2012-12-12 | 2021-05-07 | 布罗德研究所有限公司 | 用于序列操纵的系统、方法和优化的指导组合物的工程化 |
| CA2894684A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Engineering and optimization of improved crispr-cas systems, methods and enzyme compositions for sequence manipulation in eukaryotes |
| AU2013359262C1 (en) | 2012-12-12 | 2021-05-13 | Massachusetts Institute Of Technology | CRISPR-Cas component systems, methods and compositions for sequence manipulation |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| WO2014093718A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Methods, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof |
| DK2931897T3 (en) | 2012-12-12 | 2018-02-05 | Broad Inst Inc | CONSTRUCTION, MODIFICATION AND OPTIMIZATION OF SYSTEMS, PROCEDURES AND COMPOSITIONS FOR SEQUENCE MANIPULATION AND THERAPEUTICAL APPLICATIONS |
| CA2895155C (en) | 2012-12-17 | 2021-07-06 | President And Fellows Of Harvard College | Rna-guided human genome engineering |
| LT2943579T (lt) | 2013-01-10 | 2018-11-12 | Dharmacon, Inc. | Molekulių bibliotekos ir molekulių generavimo būdai |
| EP3919505B1 (en) | 2013-01-16 | 2023-08-30 | Emory University | Uses of cas9-nucleic acid complexes |
| WO2014143381A1 (en) | 2013-03-09 | 2014-09-18 | Agilent Technologies, Inc. | Methods of in vivo engineering of large sequences using multiple crispr/cas selections of recombineering events |
| MX374090B (es) | 2013-03-14 | 2025-03-05 | Caribou Biosciences Inc | Composiciones y métodos de ácidos nucleicos dirigidos al ácido nucleico. |
| US9234213B2 (en) | 2013-03-15 | 2016-01-12 | System Biosciences, Llc | Compositions and methods directed to CRISPR/Cas genomic engineering systems |
| AU2014227653B2 (en) | 2013-03-15 | 2017-04-20 | The General Hospital Corporation | Using RNA-guided foki nucleases (RFNs) to increase specificity for RNA-guided genome editing |
| JP2016522679A (ja) | 2013-04-04 | 2016-08-04 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ＣＲＩＳＰＲ／Ｃａｓ系を用いたゲノム編集の治療的使用 |
| WO2014191128A1 (en) | 2013-05-29 | 2014-12-04 | Cellectis | Methods for engineering t cells for immunotherapy by using rna-guided cas nuclease system |
| EP3011030B1 (en) * | 2013-06-17 | 2023-11-08 | The Broad Institute, Inc. | Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation |
| JP7120717B2 (ja) | 2013-07-09 | 2022-08-17 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 多重ｒｎａ誘導型ゲノム編集 |
| SMT202100691T1 (it) | 2013-07-11 | 2022-01-10 | Modernatx Inc | Composizioni 5 comprendenti polinucleotidi sintetici che codificano proteine correlate a crispr e sgrna sintetici e metodi d'uso |
| US10563225B2 (en) | 2013-07-26 | 2020-02-18 | President And Fellows Of Harvard College | Genome engineering |
| WO2015017866A1 (en) | 2013-08-02 | 2015-02-05 | Enevolv, Inc. | Processes and host cells for genome, pathway, and biomolecular engineering |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| WO2015034872A2 (en) | 2013-09-05 | 2015-03-12 | Massachusetts Institute Of Technology | Tuning microbial populations with programmable nucleases |
| EP3418379B1 (en) | 2013-09-18 | 2020-12-09 | Kymab Limited | Methods, cells & organisms |
| WO2015048577A2 (en) | 2013-09-27 | 2015-04-02 | Editas Medicine, Inc. | Crispr-related methods and compositions |
| US10822606B2 (en) | 2013-09-27 | 2020-11-03 | The Regents Of The University Of California | Optimized small guide RNAs and methods of use |
| US20150098954A1 (en) | 2013-10-08 | 2015-04-09 | Elwha Llc | Compositions and Methods Related to CRISPR Targeting |
| US10752906B2 (en) | 2013-11-05 | 2020-08-25 | President And Fellows Of Harvard College | Precise microbiota engineering at the cellular level |
| EP3865575A1 (en) | 2013-11-06 | 2021-08-18 | Hiroshima University | Vector for nucleic acid insertion |
| US11326209B2 (en) | 2013-11-07 | 2022-05-10 | Massachusetts Institute Of Technology | Cell-based genomic recorded accumulative memory |
| EP3760719A1 (en) | 2013-11-18 | 2021-01-06 | CRISPR Therapeutics AG | Crispr-cas system materials and methods |
| US9074199B1 (en) | 2013-11-19 | 2015-07-07 | President And Fellows Of Harvard College | Mutant Cas9 proteins |
| US9546384B2 (en) | 2013-12-11 | 2017-01-17 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for the targeted modification of a mouse genome |
| MX2016007325A (es) | 2013-12-12 | 2017-07-19 | Broad Inst Inc | Composiciones y metodos de uso de sistemas crispr-cas en desordenes debidos a repeticion de nucleotidos. |
| US10787654B2 (en) | 2014-01-24 | 2020-09-29 | North Carolina State University | Methods and compositions for sequence guiding Cas9 targeting |
| EP4063503A1 (en) | 2014-02-11 | 2022-09-28 | The Regents of the University of Colorado, a body corporate | Crispr enabled multiplexed genome engineering |
| WO2015122967A1 (en) | 2014-02-13 | 2015-08-20 | Clontech Laboratories, Inc. | Methods of depleting a target molecule from an initial collection of nucleic acids, and compositions and kits for practicing the same |
| WO2015153889A2 (en) | 2014-04-02 | 2015-10-08 | University Of Florida Research Foundation, Incorporated | Materials and methods for the treatment of latent viral infection |
| GB201406970D0 (en) | 2014-04-17 | 2014-06-04 | Green Biologics Ltd | Targeted mutations |
| GB201406968D0 (en) | 2014-04-17 | 2014-06-04 | Green Biologics Ltd | Deletion mutants |
| WO2015168600A2 (en) | 2014-05-02 | 2015-11-05 | Tufts University | Methods and apparatus for transformation of naturally competent cells |
| EP3152221A4 (en) | 2014-05-20 | 2018-01-24 | Regents of the University of Minnesota | Method for editing a genetic sequence |
| CN106687601A (zh) | 2014-05-28 | 2017-05-17 | 株式会社图尔金 | 使用靶特异性核酸酶灵敏检测靶dna的方法 |
| EP3152319A4 (en) | 2014-06-05 | 2017-12-27 | Sangamo BioSciences, Inc. | Methods and compositions for nuclease design |
| WO2015191693A2 (en) | 2014-06-10 | 2015-12-17 | Massachusetts Institute Of Technology | Method for gene editing |
| EP3157328B1 (en) | 2014-06-17 | 2021-08-04 | Poseida Therapeutics, Inc. | A method for directing proteins to specific loci in the genome and uses thereof |
| US20150376586A1 (en) | 2014-06-25 | 2015-12-31 | Caribou Biosciences, Inc. | RNA Modification to Engineer Cas9 Activity |
| GB201411344D0 (en) | 2014-06-26 | 2014-08-13 | Univ Leicester | Cloning |
| ES3047792T3 (en) | 2014-07-14 | 2025-12-04 | Univ California | Crispr/cas transcriptional modulation |
| US20160053272A1 (en) | 2014-07-18 | 2016-02-25 | Whitehead Institute For Biomedical Research | Methods Of Modifying A Sequence Using CRISPR |
| US20160053304A1 (en) | 2014-07-18 | 2016-02-25 | Whitehead Institute For Biomedical Research | Methods Of Depleting Target Sequences Using CRISPR |
| US20160076093A1 (en) | 2014-08-04 | 2016-03-17 | University Of Washington | Multiplex homology-directed repair |
| CN106922154B (zh) | 2014-08-06 | 2022-01-07 | 基因工具股份有限公司 | 使用空肠弯曲杆菌crispr/cas系统衍生的rna引导的工程化核酸酶的基因编辑 |
| US10513711B2 (en) | 2014-08-13 | 2019-12-24 | Dupont Us Holding, Llc | Genetic targeting in non-conventional yeast using an RNA-guided endonuclease |
| WO2016040594A1 (en) | 2014-09-10 | 2016-03-17 | The Regents Of The University Of California | Reconstruction of ancestral cells by enzymatic recording |
| WO2016057951A2 (en) | 2014-10-09 | 2016-04-14 | Life Technologies Corporation | Crispr oligonucleotides and gene editing |
| CN107429246B (zh) * | 2014-10-31 | 2021-06-01 | 麻省理工学院 | 用于crispr的大规模并行组合遗传学 |
| US10900034B2 (en) | 2014-12-03 | 2021-01-26 | Agilent Technologies, Inc. | Guide RNA with chemical modifications |
| JP6839082B2 (ja) | 2014-12-17 | 2021-03-03 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ | 環状ポリヌクレオチド修飾鋳型と組み合わせてガイドｒｎａ／ｃａｓエンドヌクレアーゼ系を用いるｅ．コリ（ｅ．ｃｏｌｉ）での効率的な遺伝子編集のための組成物および方法 |
| CA2971444A1 (en) | 2014-12-20 | 2016-06-23 | Arc Bio, Llc | Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using crispr/cas system proteins |
| GB201506509D0 (en) | 2015-04-16 | 2015-06-03 | Univ Wageningen | Nuclease-mediated genome editing |
| CN107849546A (zh) | 2015-05-15 | 2018-03-27 | 先锋国际良种公司 | 对cas内切核酸酶系统、pam序列和指导rna元件的快速表征 |
| US9790490B2 (en) * | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| TWI813532B (zh) | 2015-06-18 | 2023-09-01 | 美商博得學院股份有限公司 | 降低脱靶效應的ｃｒｉｓｐｒ酶突變 |
| EP4545544A3 (en) | 2015-06-29 | 2025-10-08 | Ionis Pharmaceuticals, Inc. | Modified crispr rna and modified single crispr rna and uses thereof |
| WO2017015015A1 (en) | 2015-07-17 | 2017-01-26 | Emory University | Crispr-associated protein from francisella and uses related thereto |
| JP6937740B2 (ja) | 2015-07-28 | 2021-09-22 | ダニスコ・ユーエス・インク | ゲノム編集システムおよび使用方法 |
| US11339408B2 (en) | 2015-08-20 | 2022-05-24 | Applied Stemcell, Inc. | Nuclease with enhanced efficiency of genome editing |
| WO2017053431A2 (en) | 2015-09-21 | 2017-03-30 | Arcturus Therapeutics, Inc. | Allele selective gene editing and uses thereof |
| EP3353309A4 (en) | 2015-09-25 | 2019-04-10 | Tarveda Therapeutics, Inc. | COMPOSITIONS AND METHOD FOR GENERIC EDITING |
| US11371040B2 (en) | 2015-10-22 | 2022-06-28 | Institut National De La Sante Et De La Recherche Medical (Inserm) | Endonuclease-barcoding |
| DK3350327T3 (en) | 2015-10-23 | 2019-01-21 | Caribou Biosciences Inc | CONSTRUCTED CRISPR CLASS-2-NUCLEIC ACID TARGETING-NUCLEIC ACID |
| CN106893739A (zh) | 2015-11-17 | 2017-06-27 | 香港中文大学 | 用于靶向基因操作的新方法和系统 |
| JP2018534942A (ja) | 2015-11-26 | 2018-11-29 | ディーエヌエーイー グループ ホールディングス リミテッド | 単一分子対照 |
| CN109310784B (zh) | 2015-12-07 | 2022-08-19 | 阿克生物公司 | 用于制备和使用指导核酸的方法和组合物 |
| JP6605042B2 (ja) | 2015-12-07 | 2019-11-13 | ザイマージェン インコーポレイテッド | Ｈｔｐゲノム操作プラットフォームによる微生物株の改良 |
| US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
| EP3394255A2 (en) | 2015-12-24 | 2018-10-31 | B.R.A.I.N. Ag | Reconstitution of dna-end repair pathway in prokaryotes |
| US10017760B2 (en) | 2016-06-24 | 2018-07-10 | Inscripta, Inc. | Methods for generating barcoded combinatorial libraries |
| US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| US10011849B1 (en) | 2017-06-23 | 2018-07-03 | Inscripta, Inc. | Nucleic acid-guided nucleases |
-
2017
- 2017-06-23 US US15/632,222 patent/US10017760B2/en active Active
- 2017-06-23 AU AU2017280353A patent/AU2017280353B2/en not_active Ceased
- 2017-06-23 CA CA3029254A patent/CA3029254A1/en active Pending
- 2017-06-23 CN CN201780052145.8A patent/CN109688820B/zh active Active
- 2017-06-23 DK DK17816357.2T patent/DK3474669T3/da active
- 2017-06-23 LT LTEPPCT/US2017/039146T patent/LT3474669T/lt unknown
- 2017-06-23 ES ES17816357T patent/ES2915562T3/es active Active
- 2017-06-23 JP JP2019519616A patent/JP2019518478A/ja active Pending
- 2017-06-23 EP EP17816357.2A patent/EP3474669B1/en active Active
- 2017-06-23 WO PCT/US2017/039146 patent/WO2017223538A1/en not_active Ceased
-
2018
- 2018-04-09 US US15/948,798 patent/US10287575B2/en active Active
- 2018-04-09 US US15/948,793 patent/US10294473B2/en active Active
-
2019
- 2019-03-07 US US16/295,393 patent/US11584928B2/en active Active
-
2023
- 2023-01-20 US US18/157,740 patent/US20230227810A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20180230461A1 (en) | 2018-08-16 |
| CN109688820B (zh) | 2023-01-10 |
| CN109688820A (zh) | 2019-04-26 |
| US10294473B2 (en) | 2019-05-21 |
| WO2017223538A1 (en) | 2017-12-28 |
| US10287575B2 (en) | 2019-05-14 |
| US10017760B2 (en) | 2018-07-10 |
| DK3474669T3 (da) | 2022-06-27 |
| JP2019518478A (ja) | 2019-07-04 |
| US20180230460A1 (en) | 2018-08-16 |
| ES2915562T3 (es) | 2022-06-23 |
| CA3029254A1 (en) | 2017-12-28 |
| AU2017280353A1 (en) | 2019-01-24 |
| US20230227810A1 (en) | 2023-07-20 |
| EP3474669B1 (en) | 2022-04-06 |
| EP3474669A4 (en) | 2019-05-08 |
| WO2017223538A9 (en) | 2018-07-19 |
| US20190194650A1 (en) | 2019-06-27 |
| US11584928B2 (en) | 2023-02-21 |
| US20170369870A1 (en) | 2017-12-28 |
| AU2017280353B2 (en) | 2021-11-11 |
| EP3474669A1 (en) | 2019-05-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| LT3474669T (lt) | Barkodu pažymėtų kombinatorinių bibliotekų generavimo būdai | |
| IL263586B (en) | Inhibitors of the menin-mll interaction | |
| PL3507418T3 (pl) | Sposoby biopochodnej derywatyzacji powierzchni celulozowych | |
| EP3322803A4 (en) | ENZYMATIC SYNTHESIS OF D-TAGATOSIS | |
| EP3411225C0 (en) | WALL STRUCTURE | |
| EP3420388A4 (en) | PUPILS EQUALIZATION | |
| PL3325443T3 (pl) | Sposób otrzymywania pridopidyny | |
| IL272851A (en) | Methods of using dipivefrin | |
| LT3645518T (lt) | Omekamitivo mekarbilo sintezė | |
| EP3406336A4 (en) | NANO FOIL OF CORE CASE STRUCTURE TYPE | |
| LT3448846T (lt) | Indazolų sintezė | |
| EP3360010A4 (en) | BI PHOTONS WITH VOTABLE SOURCE | |
| PL3457851T3 (pl) | Pochodne sobetiromu | |
| EP3753715C0 (de) | Siegelorgan | |
| DK3283210T3 (da) | Fremgangsmåde | |
| HUE049331T2 (hu) | Indazolok szintézise | |
| IL313083A (en) | Terlipressin compositions and their methods of use | |
| FR3023290B1 (fr) | Derives de flavaglines | |
| EP3277701A4 (en) | SYNTHESIS OF DESOSAMINES | |
| EP3328991A4 (en) | BIOSYNTHESIS OF POLYCETIDES | |
| EP3619920A4 (en) | AUDIO OBJECT INTERACTIONS WITHOUT METADATA | |
| IL254502A0 (en) | Solid forms of menaquinols | |
| EP3479227A4 (en) | CORRELATION OF MULTIPLE SOURCES | |
| DK3225739T3 (da) | Vægelement | |
| EP3532637A4 (en) | DETECTION PROCESS |