MA46424B1 - Identification et isolation de progéniteur otique humain - Google Patents

Identification et isolation de progéniteur otique humain

Info

Publication number
MA46424B1
MA46424B1 MA46424A MA46424A MA46424B1 MA 46424 B1 MA46424 B1 MA 46424B1 MA 46424 A MA46424 A MA 46424A MA 46424 A MA46424 A MA 46424A MA 46424 B1 MA46424 B1 MA 46424B1
Authority
MA
Morocco
Prior art keywords
otic progenitor
isolation
identification
human otic
progenitor cells
Prior art date
Application number
MA46424A
Other languages
English (en)
Other versions
MA46424A (fr
Inventor
Carlos Marcelo Nicolas Rivolta
Sarah Louise Boddy
Original Assignee
Univ Sheffield
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Sheffield filed Critical Univ Sheffield
Publication of MA46424A publication Critical patent/MA46424A/fr
Publication of MA46424B1 publication Critical patent/MA46424B1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3076—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells against structure-related tumour-associated moieties
    • C07K16/3084—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells against structure-related tumour-associated moieties against tumour-associated gangliosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70596—Molecules with a "CD"-designation not provided for elsewhere in G01N2333/705
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/71—Assays involving receptors, cell surface antigens or cell surface determinants for growth factors; for growth regulators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/745—Assays involving non-enzymic blood coagulation factors
    • G01N2333/7452—Thrombomodulin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Neurology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Neurosurgery (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Materials For Medical Uses (AREA)

Abstract

La présente invention concerne de manière générale l'identification et l'isolement de cellules progénitrices otiques humaines. Plus spécifiquement, la présente invention concerne un procédé d'utilisation de marqueurs cellulaires pour identifier et isoler des cellules progénitrices otiques humaines à partir d'une population mixte de cellules, des procédés d'enrichissement et de production de cellules progénitrices otiques humaines, et des kits associés destinés à être utilisés dans l'identification et/ou l'isolement de cellules progénitrices otiques humaines, les marqueurs cellulaires étant choisis parmi ssea1 (cd15), le disialoganglioside gd3, la tra-2-49 (foie/os/phosphatase alcaline rénale), ssea4, le ganglioside gd2 et le cd141.
MA46424A 2016-09-15 2017-09-14 Identification et isolation de progéniteur otique humain MA46424B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1615714.1A GB201615714D0 (en) 2016-09-15 2016-09-15 Otic progenitor identification and isolation
PCT/GB2017/052707 WO2018051092A1 (fr) 2016-09-15 2017-09-14 Identification et isolation de progéniteur otique humain

Publications (2)

Publication Number Publication Date
MA46424A MA46424A (fr) 2019-07-24
MA46424B1 true MA46424B1 (fr) 2021-07-29

Family

ID=57288819

Family Applications (1)

Application Number Title Priority Date Filing Date
MA46424A MA46424B1 (fr) 2016-09-15 2017-09-14 Identification et isolation de progéniteur otique humain

Country Status (28)

Country Link
US (2) US11634687B2 (fr)
EP (1) EP3513191B1 (fr)
JP (1) JP7134488B2 (fr)
KR (1) KR102409178B1 (fr)
CN (1) CN109716133B (fr)
AU (1) AU2017328903B2 (fr)
CA (1) CA3034529A1 (fr)
CL (1) CL2019000682A1 (fr)
CO (1) CO2019002095A2 (fr)
CY (1) CY1124285T1 (fr)
DK (1) DK3513191T3 (fr)
ES (1) ES2877795T3 (fr)
GB (1) GB201615714D0 (fr)
HR (1) HRP20211049T1 (fr)
HU (1) HUE055453T2 (fr)
IL (1) IL265288B2 (fr)
LT (1) LT3513191T (fr)
MA (1) MA46424B1 (fr)
MX (1) MX2019002995A (fr)
PL (1) PL3513191T3 (fr)
PT (1) PT3513191T (fr)
RS (1) RS62083B1 (fr)
RU (1) RU2768720C2 (fr)
SG (1) SG11201901410SA (fr)
SI (1) SI3513191T1 (fr)
SM (1) SMT202100378T1 (fr)
WO (1) WO2018051092A1 (fr)
ZA (1) ZA201902342B (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201615714D0 (en) * 2016-09-15 2016-11-02 Univ Of Sheffield The Otic progenitor identification and isolation
GB202213060D0 (en) 2022-09-07 2022-10-19 Rinri Therapeutics Ltd Surgical method for treatment of auditory disease or condition
GB202214291D0 (en) 2022-09-29 2022-11-16 Rinri Therapeutics Ltd Laminin culture protocol
CN116162589B (zh) * 2022-12-30 2025-10-03 西北工业大学 干细胞及其制备方法和应用、及标记组合物及其应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003524407A (ja) 1999-07-20 2003-08-19 ユニバーシティ オブ サザン カリフォルニア 多能性前−間葉、前−造血先祖幹細胞の同定
US6436704B1 (en) 2000-04-10 2002-08-20 Raven Biotechnologies, Inc. Human pancreatic epithelial progenitor cells and methods of isolation and use thereof
US6534052B1 (en) 2000-09-05 2003-03-18 Yong-Fu Xiao Cardiac function comprising implantation of embryonic stem cell in which differentiation has been initiated
DE60230774D1 (de) 2001-04-20 2009-02-26 Children S Hospital Of Orange Isolierung von nervenstammzellen unter verwendung von gangliosiden und anderen oberflächenmarkern
US8673634B2 (en) 2003-11-13 2014-03-18 Massachusetts Eye & Ear Infirmary Method for the treatment of hearing loss
US20070250942A1 (en) 2006-04-07 2007-10-25 Cold Spring Harbor Laboratory Non-human transgenic mammals useful for identifying and assessing neural stem/progenitor cells
WO2008132722A1 (fr) * 2007-04-26 2008-11-06 Ramot At Tel-Aviv University Ltd. Cellules souches autologues pluripotentes de muqueuse buccale et procédé d'utilisation
GB2450747A (en) 2007-07-06 2009-01-07 Univ Sheffield Treatment of sensorineural hearing loss
RU2525223C2 (ru) * 2009-06-08 2014-08-10 Иалоджик Кореа Инк. Способ стимулирования области волосковых сенсорных клеток
WO2012103012A1 (fr) 2011-01-24 2012-08-02 The Board Of Trustees Of The Leland Stanford Junior University Procédés de génération de cellules de l'oreille interne in vitro
BR112014019328A8 (pt) 2012-02-17 2017-07-11 The Schepens Eye Res Institute Perfil de fenótipo de células progenitoras retinais humanas
WO2013188679A1 (fr) 2012-06-13 2013-12-19 Stemgent, Inc. Méthodes de préparation de cellules souches pluripotentes
GB201615714D0 (en) * 2016-09-15 2016-11-02 Univ Of Sheffield The Otic progenitor identification and isolation

Also Published As

Publication number Publication date
RS62083B1 (sr) 2021-08-31
RU2019110990A3 (fr) 2020-12-09
US20230313134A1 (en) 2023-10-05
RU2019110990A (ru) 2020-10-15
HUE055453T2 (hu) 2021-11-29
CL2019000682A1 (es) 2019-06-07
RU2768720C2 (ru) 2022-03-24
MA46424A (fr) 2019-07-24
PT3513191T (pt) 2021-07-05
SMT202100378T1 (it) 2021-09-14
JP2019533138A (ja) 2019-11-14
US11634687B2 (en) 2023-04-25
JP7134488B2 (ja) 2022-09-12
IL265288B2 (en) 2024-03-01
PL3513191T3 (pl) 2021-10-25
BR112019004749A2 (pt) 2019-05-28
AU2017328903A1 (en) 2019-03-07
SG11201901410SA (en) 2019-03-28
SI3513191T1 (sl) 2021-09-30
MX2019002995A (es) 2019-09-18
AU2017328903B2 (en) 2023-07-06
US12209254B2 (en) 2025-01-28
ZA201902342B (en) 2021-09-29
CA3034529A1 (fr) 2018-03-22
CN109716133A (zh) 2019-05-03
CN109716133B (zh) 2023-04-28
HRP20211049T1 (hr) 2021-10-01
LT3513191T (lt) 2021-08-10
IL265288A (en) 2019-05-30
DK3513191T3 (da) 2021-07-05
CY1124285T1 (el) 2022-07-22
EP3513191B1 (fr) 2021-04-28
ES2877795T3 (es) 2021-11-17
WO2018051092A1 (fr) 2018-03-22
EP3513191A1 (fr) 2019-07-24
KR102409178B1 (ko) 2022-06-14
IL265288B1 (en) 2023-11-01
CO2019002095A2 (es) 2019-05-31
KR20190051971A (ko) 2019-05-15
US20190185813A1 (en) 2019-06-20
GB201615714D0 (en) 2016-11-02

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