MA69005B1 - Compositions et procédés pour la stabilisation de micro-arn - Google Patents

Compositions et procédés pour la stabilisation de micro-arn

Info

Publication number
MA69005B1
MA69005B1 MA69005A MA69005A MA69005B1 MA 69005 B1 MA69005 B1 MA 69005B1 MA 69005 A MA69005 A MA 69005A MA 69005 A MA69005 A MA 69005A MA 69005 B1 MA69005 B1 MA 69005B1
Authority
MA
Morocco
Prior art keywords
microrna
compositions
stabilizing
methods
micrornas
Prior art date
Application number
MA69005A
Other languages
English (en)
Inventor
Zouher Majd
Original Assignee
Genfit
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genfit filed Critical Genfit
Publication of MA69005B1 publication Critical patent/MA69005B1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/30—Characterised by physical treatment
    • C12Q2523/31—Electrostatic interactions, e.g. use of cationic polymers in hybridisation reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/207—Modifications characterised by siRNA, miRNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/143—Concentration of primer or probe
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/101—Reactions characterised by their quantitative nature the purpose being quantitative analysis with an internal standard/control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/107—Reactions characterised by their quantitative nature the purpose being quantitative analysis with a competitive internal standard/control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Steroid Compounds (AREA)

Abstract

La présente invention concerne la stabilisation de molécules de micro-ARN. Les compositions et méthodes décrites ici peuvent être avantageusement utilisées pour la fourniture de microARN de contrôle interne et standard destinés à être inclus dans des kits, utiles pour la quantification normalisée, relative ou absolue d'un microARN dans un fluide biologique.
MA69005A 2019-04-16 2020-04-16 Compositions et procédés pour la stabilisation de micro-arn MA69005B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19305496 2019-04-16
EP23164521.9A EP4223886B1 (fr) 2019-04-16 2020-04-16 Compositions et procédés pour la stabilisation de micro-arn

Publications (1)

Publication Number Publication Date
MA69005B1 true MA69005B1 (fr) 2025-01-31

Family

ID=66334331

Family Applications (1)

Application Number Title Priority Date Filing Date
MA69005A MA69005B1 (fr) 2019-04-16 2020-04-16 Compositions et procédés pour la stabilisation de micro-arn

Country Status (18)

Country Link
US (1) US20220186313A1 (fr)
EP (2) EP3956470A1 (fr)
JP (2) JP2022529035A (fr)
KR (1) KR20210153077A (fr)
CN (1) CN113661251B (fr)
AU (1) AU2020259847B2 (fr)
DK (1) DK4223886T3 (fr)
EA (1) EA202192832A1 (fr)
ES (1) ES3005085T3 (fr)
FI (1) FI4223886T3 (fr)
IL (1) IL286580B2 (fr)
MA (1) MA69005B1 (fr)
MX (1) MX2021012610A (fr)
PL (1) PL4223886T3 (fr)
PT (1) PT4223886T (fr)
TW (1) TWI866964B (fr)
WO (1) WO2020212522A1 (fr)
ZA (1) ZA202109058B (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12458612B2 (en) 2019-04-10 2025-11-04 Genfit Combination therapy comprising compounds of formula (I) and GLP-1 receptor agonists
GB2594317B (en) * 2020-04-23 2022-05-25 Univ Johannesburg Witwatersrand SARS-CoV-2 Diagnostic control compositions
US20220340962A1 (en) * 2021-04-22 2022-10-27 Genfit Quantitative detection of micro-rnas
CN115737557B (zh) * 2022-11-18 2024-04-09 黑龙江中医药大学 一种miR-55制剂及其制备方法和应用

Family Cites Families (22)

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Publication number Priority date Publication date Assignee Title
US5334761A (en) 1992-08-28 1994-08-02 Life Technologies, Inc. Cationic lipids
JPH09500013A (ja) 1993-06-01 1997-01-07 ライフ・テクノロジーズ・インコーポレイテッド カチオン性脂質による遺伝子免疫
US5578475A (en) 1993-07-12 1996-11-26 Life Technologies, Inc. Composition and methods for transfecting eukaryotic cells
US5674908A (en) 1993-12-20 1997-10-07 Life Technologies, Inc. Highly packed polycationic ammonium, sulfonium and phosphonium lipids
US6051429A (en) 1995-06-07 2000-04-18 Life Technologies, Inc. Peptide-enhanced cationic lipid transfections
US20030069173A1 (en) 1998-03-16 2003-04-10 Life Technologies, Inc. Peptide-enhanced transfections
JP4338106B2 (ja) 1995-06-07 2009-10-07 ライフ テクノロジーズ コーポレーション ペプチド増強カチオン脂質トランスフェクション
ES2296419T3 (es) 1998-11-12 2008-04-16 Invitrogen Corporation Reactivos de transfeccion.
CA2427068A1 (fr) 2000-10-27 2002-05-02 Invitrogen Corporation Procede d'introduction d'oligonucleotides antisens dans des cellules eucaryotes
GB0313132D0 (en) * 2003-06-06 2003-07-09 Ich Productions Ltd Peptide ligands
US7404969B2 (en) * 2005-02-14 2008-07-29 Sirna Therapeutics, Inc. Lipid nanoparticle based compositions and methods for the delivery of biologically active molecules
US20060228406A1 (en) 2005-03-17 2006-10-12 Invitrogen Corporation Transfection reagent for non-adherent suspension cells
EP2545190A1 (fr) * 2010-03-11 2013-01-16 National University of Ireland Galway Détection et quantification de microarn dans la circulation et utilisation de microarn circulants en tant que biomarqueurs pour le cancer
BR122022001262B1 (pt) * 2010-11-15 2022-09-27 Life Technologies Corporation Compostos de transfecção contendo amina e complexo de transfecção
CN103205483B (zh) * 2012-01-13 2017-02-08 北京命码生科科技有限公司 microRNA标准化的内参基因及其用途
US20150080229A1 (en) * 2012-03-29 2015-03-19 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Plasma microribonucleic acids as biomarkers for endometriosis and endometriosis-associated ovarian cancer
WO2013148151A1 (fr) * 2012-03-29 2013-10-03 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Acides microribonucléiques plasmatiques à titre de biomarqueurs de l'endométriose et du cancer de l'ovaire associé à l'endométriose (eaoc)
CN106572974B (zh) 2014-07-15 2021-04-23 生命技术公司 用于将分子有效递送到细胞的具有脂质聚集体的组合物和方法
WO2017029556A1 (fr) * 2015-08-20 2017-02-23 Asociación Centro De Investigación Cooperativa En Biociencias - Cis Biogune Méthodes et compositions pour traiter des maladies et des troubles hépatiques
JP2017093377A (ja) * 2015-11-26 2017-06-01 国立大学法人 東京医科歯科大学 肝がんモデルマウスおよび肝がん治療用の医薬
CN109072302B (zh) 2016-03-30 2024-01-16 基恩菲特公司 非酒精性脂肪性肝炎的非侵入性诊断
LT3675826T (lt) 2017-08-31 2023-09-11 Life Technologies Corporation Katijoninės lipidų kompozicijos audiniams-specifiniam įterpimui

Also Published As

Publication number Publication date
AU2020259847A1 (en) 2021-11-04
EA202192832A1 (ru) 2022-01-19
EP3956470A1 (fr) 2022-02-23
IL286580B1 (en) 2024-09-01
KR20210153077A (ko) 2021-12-16
ZA202109058B (en) 2025-05-28
AU2020259847B2 (en) 2024-09-19
TWI866964B (zh) 2024-12-21
TW202104599A (zh) 2021-02-01
FI4223886T3 (fi) 2024-12-27
DK4223886T3 (da) 2025-01-02
JP2022529035A (ja) 2022-06-16
CN113661251A (zh) 2021-11-16
ES3005085T3 (en) 2025-03-13
BR112021020664A2 (pt) 2021-12-07
EP4223886A3 (fr) 2023-09-13
WO2020212522A1 (fr) 2020-10-22
US20220186313A1 (en) 2022-06-16
IL286580A (en) 2021-10-31
IL286580B2 (en) 2025-01-01
EP4223886A2 (fr) 2023-08-09
CA3130522A1 (fr) 2020-10-22
PL4223886T3 (pl) 2025-03-31
CN113661251B (zh) 2024-08-23
EP4223886B1 (fr) 2024-10-02
JP2025067916A (ja) 2025-04-24
MX2021012610A (es) 2021-11-12
PT4223886T (pt) 2024-12-20

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