MD4586C1 - Спектрофотометрический метод определения общего селена в биомассе водорослей - Google Patents
Спектрофотометрический метод определения общего селена в биомассе водорослей Download PDFInfo
- Publication number
- MD4586C1 MD4586C1 MDA20160088A MD20160088A MD4586C1 MD 4586 C1 MD4586 C1 MD 4586C1 MD A20160088 A MDA20160088 A MD A20160088A MD 20160088 A MD20160088 A MD 20160088A MD 4586 C1 MD4586 C1 MD 4586C1
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- MD
- Moldova
- Prior art keywords
- selenium
- solution
- ions
- supernatant
- added
- Prior art date
Links
- 239000011669 selenium Substances 0.000 title claims abstract description 51
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 35
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 35
- 239000002028 Biomass Substances 0.000 title claims abstract description 13
- 238000002798 spectrophotometry method Methods 0.000 title claims abstract description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 18
- 229920002472 Starch Polymers 0.000 claims abstract description 15
- 239000008107 starch Substances 0.000 claims abstract description 15
- -1 Cu2+ ions Chemical class 0.000 claims abstract description 12
- 235000019698 starch Nutrition 0.000 claims abstract description 10
- 238000011088 calibration curve Methods 0.000 claims abstract description 6
- 239000006228 supernatant Substances 0.000 claims description 12
- 238000002835 absorbance Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 239000002244 precipitate Substances 0.000 claims description 4
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 3
- 229910017604 nitric acid Inorganic materials 0.000 claims description 3
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 2
- 238000000605 extraction Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 11
- 238000001556 precipitation Methods 0.000 abstract description 3
- 230000033558 biomineral tissue development Effects 0.000 abstract description 2
- 150000001768 cations Chemical class 0.000 abstract 1
- 230000000873 masking effect Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 29
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Substances [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 12
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 10
- 235000016425 Arthrospira platensis Nutrition 0.000 description 6
- 240000002900 Arthrospira platensis Species 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 239000000523 sample Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 239000012496 blank sample Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 3
- 229940082787 spirulina Drugs 0.000 description 3
- 229910003424 Na2SeO3 Inorganic materials 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 229960005070 ascorbic acid Drugs 0.000 description 2
- 235000010323 ascorbic acid Nutrition 0.000 description 2
- 239000011668 ascorbic acid Substances 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000011781 sodium selenite Substances 0.000 description 2
- 235000015921 sodium selenite Nutrition 0.000 description 2
- 239000012224 working solution Substances 0.000 description 2
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 241001464430 Cyanobacterium Species 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010063493 Premature ageing Diseases 0.000 description 1
- 208000032038 Premature aging Diseases 0.000 description 1
- 102000008114 Selenoproteins Human genes 0.000 description 1
- 108010074686 Selenoproteins Proteins 0.000 description 1
- AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical class IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 239000008139 complexing agent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- SPVXKVOXSXTJOY-UHFFFAOYSA-N selane Chemical compound [SeH2] SPVXKVOXSXTJOY-UHFFFAOYSA-N 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000005495 thyroid hormone Substances 0.000 description 1
- 229940036555 thyroid hormone Drugs 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
Изобретение относится к аналитический химии и микробиологии, а именно к спектрофотометрическому методу определения общего селена в биомассе водорослей.Метод включает минерализацию образца, добавление гидроксида натрия для осаждения ионов Fe3+ и Cu2+, маскирование оставшихся ионов Fe3+ и Cu2+ посредством их связывания к катиониту, добавление растворов 4,5 М HCl, 0,25% KI, 0,2% крахмала и определение концентрации селена с использованием калибровочной кривой.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20160088A MD4586C1 (ru) | 2016-07-21 | 2016-07-21 | Спектрофотометрический метод определения общего селена в биомассе водорослей |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20160088A MD4586C1 (ru) | 2016-07-21 | 2016-07-21 | Спектрофотометрический метод определения общего селена в биомассе водорослей |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| MD20160088A2 MD20160088A2 (ru) | 2018-01-31 |
| MD4586B1 MD4586B1 (ru) | 2018-07-31 |
| MD4586C1 true MD4586C1 (ru) | 2019-02-28 |
Family
ID=61072132
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDA20160088A MD4586C1 (ru) | 2016-07-21 | 2016-07-21 | Спектрофотометрический метод определения общего селена в биомассе водорослей |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD4586C1 (ru) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU689942A1 (en) * | 1977-06-01 | 1979-10-05 | Revold P Pantaler | Method of selenium determination |
| MD659G2 (ru) * | 1995-10-16 | 1997-07-31 | Институт Микробиологии И Биотехнологии Академии Наук Молдовы | Спектрофотометрический метод определения селена |
-
2016
- 2016-07-21 MD MDA20160088A patent/MD4586C1/ru not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| MD4586B1 (ru) | 2018-07-31 |
| MD20160088A2 (ru) | 2018-01-31 |
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|---|---|---|---|
| FG4A | Patent for invention issued | ||
| KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) | ||
| MM4A | Patent for invention definitely lapsed due to non-payment of fees |