MX2022008045A - Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu). - Google Patents

Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu).

Info

Publication number
MX2022008045A
MX2022008045A MX2022008045A MX2022008045A MX2022008045A MX 2022008045 A MX2022008045 A MX 2022008045A MX 2022008045 A MX2022008045 A MX 2022008045A MX 2022008045 A MX2022008045 A MX 2022008045A MX 2022008045 A MX2022008045 A MX 2022008045A
Authority
MX
Mexico
Prior art keywords
umis
sequences
unique molecular
dna fragments
sequenced dna
Prior art date
Application number
MX2022008045A
Other languages
English (en)
Inventor
Emrah Kostem
Sante Gnerre
Alex Aravanis
Byoungsok Jung
Zhihong Zhang
Xuyu Cai
Alex So
Frank J Steemers
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=55910388&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2022008045(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2022008045A publication Critical patent/MX2022008045A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/119—Double strand sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Theoretical Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Las realizaciones desveladas se refieren a métodos, aparatos, sistemas y productos de programa informático para determinar secuencias de interés usando secuencias de índice molecular único (IMU) que son asociables de forma única a fragmentos polinucleotídicos individuales, que incluyen secuencias con bajas frecuencias alélicas y longitud de secuencia larga. En algunas implementaciones, los IMU incluyen tanto IMU físicos (exógenos), por ejemplo introducidos usando adaptadores en forma de Y, como IMU virtuales (endógenos) presentes en los fragmentos de ADN para secuenciar. En algunas implementaciones, las secuencias de índice molecular único incluyen secuencias no aleatorias. También se proporcionan sistemas, aparatos y productos de programa informático para determinar una secuencia de interés implementando los métodos desvelados.
MX2022008045A 2015-04-28 2017-10-26 Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu). MX2022008045A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562153699P 2015-04-28 2015-04-28
US201562193469P 2015-07-16 2015-07-16
US201562269485P 2015-12-18 2015-12-18

Publications (1)

Publication Number Publication Date
MX2022008045A true MX2022008045A (es) 2022-07-27

Family

ID=55910388

Family Applications (3)

Application Number Title Priority Date Filing Date
MX2017013775A MX2017013775A (es) 2015-04-28 2016-04-20 Supresión de error en fragmentos de adn secuenciados usando lecturas redundantes con índices moleculares únicos (imu).
MX2022008045A MX2022008045A (es) 2015-04-28 2017-10-26 Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu).
MX2023004394A MX2023004394A (es) 2015-04-28 2017-10-26 Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu).

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2017013775A MX2017013775A (es) 2015-04-28 2016-04-20 Supresión de error en fragmentos de adn secuenciados usando lecturas redundantes con índices moleculares únicos (imu).

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2023004394A MX2023004394A (es) 2015-04-28 2017-10-26 Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu).

Country Status (18)

Country Link
US (3) US10844428B2 (es)
EP (4) EP3736341B1 (es)
JP (1) JP6685324B2 (es)
KR (1) KR102091312B1 (es)
CN (2) CN108138227B (es)
AU (3) AU2016256351B2 (es)
CA (2) CA2983935C (es)
DK (2) DK3289097T4 (es)
ES (3) ES2961338T3 (es)
FI (2) FI3289097T4 (es)
IL (3) IL285319B2 (es)
MX (3) MX2017013775A (es)
MY (1) MY181983A (es)
NZ (1) NZ736609A (es)
RU (1) RU2704286C2 (es)
SG (2) SG11201708859XA (es)
WO (1) WO2016176091A1 (es)
ZA (1) ZA201707231B (es)

Families Citing this family (170)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US10083273B2 (en) 2005-07-29 2018-09-25 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US10081839B2 (en) 2005-07-29 2018-09-25 Natera, Inc System and method for cleaning noisy genetic data and determining chromosome copy number
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
WO2011041485A1 (en) 2009-09-30 2011-04-07 Gene Security Network, Inc. Methods for non-invasive prenatal ploidy calling
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
WO2011146632A1 (en) 2010-05-18 2011-11-24 Gene Security Network Inc. Methods for non-invasive prenatal ploidy calling
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
CN103608466B (zh) 2010-12-22 2020-09-18 纳特拉公司 非侵入性产前亲子鉴定方法
RU2671980C2 (ru) 2011-02-09 2018-11-08 Натера, Инк. Способы неинвазивного пренатального установления плоидности
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
ES2986436T3 (es) 2011-04-15 2024-11-11 Univ Johns Hopkins Sistema de secuenciación segura
WO2013123442A1 (en) 2012-02-17 2013-08-22 Fred Hutchinson Cancer Research Center Compositions and methods for accurately identifying mutations
PT2828218T (pt) 2012-03-20 2020-11-11 Univ Washington Through Its Center For Commercialization Métodos para baixar a taxa de erro da sequenciação paralela massiva de adn utilizando sequenciação duplex de consensus
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
CN104781421B (zh) 2012-09-04 2020-06-05 夸登特健康公司 检测稀有突变和拷贝数变异的系统和方法
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
ES2886507T5 (es) 2012-10-29 2024-11-15 Univ Johns Hopkins Prueba de Papanicolaou para cánceres de ovario y de endometrio
US10577655B2 (en) 2013-09-27 2020-03-03 Natera, Inc. Cell free DNA diagnostic testing standards
US10262755B2 (en) 2014-04-21 2019-04-16 Natera, Inc. Detecting cancer mutations and aneuploidy in chromosomal segments
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US10767222B2 (en) 2013-12-11 2020-09-08 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
WO2017062863A1 (en) 2015-10-09 2017-04-13 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
ES2660989T3 (es) 2013-12-28 2018-03-27 Guardant Health, Inc. Métodos y sistemas para detectar variantes genéticas
CA2945962C (en) 2014-04-21 2023-08-29 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
US11479812B2 (en) 2015-05-11 2022-10-25 Natera, Inc. Methods and compositions for determining ploidy
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
WO2017096322A1 (en) 2015-12-03 2017-06-08 Accuragen Holdings Limited Methods and compositions for forming ligation products
CA3006792A1 (en) 2015-12-08 2017-06-15 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
SG11201805119QA (en) 2015-12-17 2018-07-30 Guardant Health Inc Methods to determine tumor gene copy number by analysis of cell-free dna
ES2924487T3 (es) * 2016-01-29 2022-10-07 Hoffmann La Roche Un adaptador de conformación en Y novedoso para secuenciación de ácidos nucleicos y procedimiento de uso
US11514289B1 (en) 2016-03-09 2022-11-29 Freenome Holdings, Inc. Generating machine learning models using genetic data
US11384382B2 (en) 2016-04-14 2022-07-12 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids
ES3002715T3 (en) 2016-04-14 2025-03-07 Guardant Health Inc Methods for early detection of cancer
RU2760913C2 (ru) 2016-04-15 2021-12-01 Натера, Инк. Способы выявления рака легкого
CN109511265B (zh) 2016-05-16 2023-07-14 安可济控股有限公司 通过链鉴定改进测序的方法
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US11708574B2 (en) 2016-06-10 2023-07-25 Myriad Women's Health, Inc. Nucleic acid sequencing adapters and uses thereof
US10144962B2 (en) 2016-06-30 2018-12-04 Grail, Inc. Differential tagging of RNA for preparation of a cell-free DNA/RNA sequencing library
CN109844133A (zh) 2016-08-15 2019-06-04 安可济控股有限公司 检测罕见序列变体的组合物和方法
WO2018067517A1 (en) 2016-10-04 2018-04-12 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
MX2019007444A (es) 2016-12-22 2019-08-16 Guardant Health Inc Metodos y sistemas para analisis de moleculas de acido nucleico.
WO2018119399A1 (en) * 2016-12-23 2018-06-28 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
BR112019013391A2 (pt) * 2016-12-28 2020-03-03 Quest Diagnostics Investments Llc Adaptador de ácido nucleico, e, método para detecção de uma mutação em uma molécula de dna circulante tumoral (ctdna) de fita dupla.
SG11201906428SA (en) 2017-01-18 2019-08-27 Illumina Inc Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
US10968447B2 (en) 2017-01-31 2021-04-06 Myriad Women's Health, Inc. Methods and compositions for enrichment of target polynucleotides
WO2018144217A1 (en) 2017-01-31 2018-08-09 Counsyl, Inc. Methods and compositions for enrichment of target polynucleotides
WO2018144159A1 (en) * 2017-01-31 2018-08-09 Counsyl, Inc. Capture probes using positive and negative strands for duplex sequencing
WO2018148289A2 (en) * 2017-02-08 2018-08-16 Integrated Dna Technologies, Inc. Duplex adapters and duplex sequencing
WO2018156418A1 (en) 2017-02-21 2018-08-30 Natera, Inc. Compositions, methods, and kits for isolating nucleic acids
US11274344B2 (en) 2017-03-30 2022-03-15 Grail, Inc. Enhanced ligation in sequencing library preparation
WO2018183942A1 (en) * 2017-03-31 2018-10-04 Grail, Inc. Improved library preparation and use thereof for sequencing-based error correction and/or variant identification
WO2018183897A1 (en) 2017-03-31 2018-10-04 Grail, Inc. Higher target capture efficiency using probe extension
AU2018261332B2 (en) * 2017-05-01 2024-12-05 Illumina, Inc. Optimal index sequences for multiplex massively parallel sequencing
EP3622089B1 (en) * 2017-05-08 2024-07-17 Illumina, Inc. Method for sequencing using universal short adapters for indexing of polynucleotide samples
US20200080143A1 (en) * 2017-05-14 2020-03-12 Foresee Genomic Ltd Dna construct for sequencing and method for preparing the same
CN110719959B (zh) 2017-06-05 2021-08-06 贝克顿迪金森公司 针对单细胞的样品索引
ES3055990T3 (en) * 2017-06-20 2026-02-17 Illumina Inc Methods for addressing inefficiencies in amplification reactions
CA3068273A1 (en) * 2017-06-21 2018-12-27 Bluedot Llc Systems and methods for identification of nucleic acids in a sample
WO2019023924A1 (en) * 2017-08-01 2019-02-07 Helitec Limited METHODS FOR ENRICHMENT AND DETERMINATION OF TARGET NUCLEOTIDE SEQUENCES
CN111094583A (zh) 2017-08-04 2020-05-01 十亿至一公司 与生物靶相关的定量中利用靶相关分子的测序输出确定和分析
US11519024B2 (en) 2017-08-04 2022-12-06 Billiontoone, Inc. Homologous genomic regions for characterization associated with biological targets
WO2019028462A1 (en) 2017-08-04 2019-02-07 Billiontoone, Inc. TARGET-ASSOCIATED MOLECULES FOR CHARACTERIZATION ASSOCIATED WITH BIOLOGICAL TARGETS
CN111868260B (zh) 2017-08-07 2025-02-21 约翰斯霍普金斯大学 用于评估和治疗癌症的方法和材料
US11447818B2 (en) * 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
KR102878365B1 (ko) * 2017-10-09 2025-10-31 주식회사 마크로젠 단일 분자 서열분석 및 핵산서열 특성화를 위한 고유한 분자 식별자
CN107604046B (zh) * 2017-11-03 2021-08-24 上海交通大学 用于微量dna超低频突变检测的双分子自校验文库制备及杂交捕获的二代测序方法
CN118126816A (zh) * 2017-11-06 2024-06-04 伊鲁米那股份有限公司 核酸索引化技术
AU2018366213B2 (en) * 2017-11-08 2025-05-15 Twinstrand Biosciences, Inc. Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
CN110870016B (zh) * 2017-11-30 2024-09-06 伊鲁米那股份有限公司 用于序列变体呼出的验证方法和系统
KR101967879B1 (ko) * 2017-11-30 2019-04-10 사회복지법인 삼성생명공익재단 핵산 서열분석에서 uid 핵산 서열의 순결도를 측정하는 방법
US12084720B2 (en) 2017-12-14 2024-09-10 Natera, Inc. Assessing graft suitability for transplantation
EP4650456A3 (en) 2018-01-05 2026-01-21 BillionToOne, Inc. Quality control templates for ensuring validity of sequencing-based assays
US12398389B2 (en) 2018-02-15 2025-08-26 Natera, Inc. Methods for isolating nucleic acids with size selection
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
DE202019005627U1 (de) 2018-04-02 2021-05-31 Grail, Inc. Methylierungsmarker und gezielte Methylierungssondenpanels
US12024738B2 (en) 2018-04-14 2024-07-02 Natera, Inc. Methods for cancer detection and monitoring
WO2019204208A1 (en) * 2018-04-16 2019-10-24 Memorial Sloan Kettering Cancer Center SYSTEMS AND METHODS FOR DETECTING CANCER VIA cfDNA SCREENING
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
JP7358388B2 (ja) * 2018-05-03 2023-10-10 ベクトン・ディキンソン・アンド・カンパニー 反対側の転写物末端における分子バーコーディング
CN108486230B (zh) * 2018-05-18 2022-02-08 中国人民解放军陆军军医大学第一附属医院 用于无创检测mitf基因突变的试剂盒及其制备方法
CN108949951B (zh) * 2018-05-18 2022-01-28 中国人民解放军陆军军医大学第一附属医院 一种同时无创检测gjb2和slc26a4基因突变的方法和试剂盒
CN108841946B (zh) * 2018-05-18 2022-03-22 中国人民解放军陆军军医大学第一附属医院 一种无创检测gjb2基因突变的方法，试剂盒及其制备方法
CN108642173B (zh) * 2018-05-18 2022-03-22 中国人民解放军陆军军医大学第一附属医院 一种无创检测slc26a4基因突变的方法和试剂盒
CN108753934B (zh) * 2018-05-18 2022-01-28 中国人民解放军陆军军医大学第一附属医院 一种检测基因突变的方法、试剂盒及其制备方法
CN108531583B (zh) * 2018-05-18 2022-05-17 中国人民解放军陆军军医大学第一附属医院 用于无创检测mitf基因突变的引物组合及检测方法
WO2019241290A1 (en) 2018-06-12 2019-12-19 Accuragen Holdings Limited Methods and compositions for forming ligation products
CN108949941A (zh) * 2018-06-25 2018-12-07 北京莲和医学检验所有限公司 低频突变检测方法、试剂盒和装置
GB201810901D0 (en) * 2018-07-03 2018-08-15 Ucb Biopharma Sprl Method
CN110669823B (zh) * 2018-07-03 2022-05-24 中国医学科学院肿瘤医院 一种同时检测多种肝癌常见突变的ctDNA文库构建和测序数据分析方法
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
BR112021000409A2 (pt) 2018-07-12 2021-04-06 Twinstrand Biosciences, Inc. Métodos e reagentes para caracterizar edição genômica, expansão clonal e aplicações associadas
EP3833776A4 (en) 2018-08-06 2022-04-27 Billiontoone, Inc. DILUTION MARKER FOR QUANTIFICATION OF BIOLOGICAL TARGETS
US11600360B2 (en) * 2018-08-20 2023-03-07 Microsoft Technology Licensing, Llc Trace reconstruction from reads with indeterminant errors
WO2020043803A1 (en) * 2018-08-28 2020-03-05 Sophia Genetics S.A. Methods for asymmetric dna library generation and optionally integrated duplex sequencing
WO2020069350A1 (en) 2018-09-27 2020-04-02 Grail, Inc. Methylation markers and targeted methylation probe panel
JP2022504078A (ja) * 2018-10-04 2022-01-13 ブルースター ジェノミクス， インコーポレイテッド 単一の生物学的サンプル由来のタンパク質、ヌクレオソームおよび無細胞核酸のシーケンシングベースの同時分析
EP3670670A1 (en) 2018-12-18 2020-06-24 Ricoh Company, Ltd. Nucleic acid analysis method, nucleic acid analysis program, and device for library preparation
CN109706219A (zh) * 2018-12-20 2019-05-03 臻和（北京）科技有限公司 构建测序文库的方法、试剂盒、上机方法及测序数据的拆分方法
EP4538389A3 (en) * 2019-01-03 2025-07-30 Ncan Genomics, Inc. Linked target capture
WO2020167920A1 (en) 2019-02-14 2020-08-20 Cellular Research, Inc. Hybrid targeted and whole transcriptome amplification
CA3131514A1 (en) * 2019-02-25 2020-09-03 Twist Bioscience Corporation Compositions and methods for next generation sequencing
WO2020226528A1 (ru) * 2019-05-08 2020-11-12 Общество с ограниченной ответственностью "ГЕНОТЕК ИТ" Способ определения кариотипа плода беременной женщины
US12305235B2 (en) 2019-06-06 2025-05-20 Natera, Inc. Methods for detecting immune cell DNA and monitoring immune system
CN110409001B (zh) * 2019-07-25 2022-11-15 北京贝瑞和康生物技术有限公司 一种构建捕获文库的方法和试剂盒
EP3795685A1 (en) * 2019-09-20 2021-03-24 Sophia Genetics S.A. Methods for dna library generation to facilitate the detection and reporting of low frequency variants
US10927409B1 (en) 2019-10-14 2021-02-23 Pioneer Hi-Bred International, Inc. Detection of sequences uniquely associated with a dna target region
CN112176419B (zh) * 2019-10-16 2022-03-22 中国医学科学院肿瘤医院 一种检测ctDNA中肿瘤特异基因的变异和甲基化的方法
SG11202109079YA (en) 2019-12-05 2021-09-29 Illumina Inc Rapid detection of gene fusions
CN111073961A (zh) * 2019-12-20 2020-04-28 苏州赛美科基因科技有限公司 一种基因稀有突变的高通量检测方法
US20230129075A1 (en) * 2020-01-13 2023-04-27 St. Jude Children's Research Hospital Error suppression in genetic sequencing
WO2021146219A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
EP3859012A1 (en) 2020-02-03 2021-08-04 Albert-Ludwigs-Universität Freiburg Methods for amplification of genomic dna and preparation of sequencing libraries
EP4103748A4 (en) 2020-02-14 2024-03-13 The Johns Hopkins University METHOD AND MATERIALS FOR ASSESSING NUCLEIC ACIDS
US11211147B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Estimation of circulating tumor fraction using off-target reads of targeted-panel sequencing
US11475981B2 (en) 2020-02-18 2022-10-18 Tempus Labs, Inc. Methods and systems for dynamic variant thresholding in a liquid biopsy assay
US11211144B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Methods and systems for refining copy number variation in a liquid biopsy assay
CN111304288A (zh) * 2020-02-18 2020-06-19 江苏先声医学诊断有限公司 特异性分子标签umi组及其应用
WO2021173719A1 (en) 2020-02-25 2021-09-02 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
EP3892737A1 (en) * 2020-04-09 2021-10-13 Takeda Vaccines, Inc. Qualitative and quantitative determination of single virus haplotypes in complex samples
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
MX2023001400A (es) 2020-09-11 2023-04-25 Illumina Cambridge Ltd Metodos para enriquecer una secuencia diana a partir de una genoteca de secuenciación usando adaptadores de horquilla.
KR20230091116A (ko) 2020-10-21 2023-06-22 일루미나, 인코포레이티드 다수의 삽입체를 포함하는 시퀀싱 주형, 및 시퀀싱 처리량을 개선하기 위한 조성물 및 방법
US20230374592A1 (en) 2020-11-19 2023-11-23 The Usa, As Represented By The Secretary, Dept. Of Health And Human Services Massively paralleled multi-patient assay for pathogenic infection diagnosis and host physiology surveillance using nucleic acid sequencing
WO2022125100A1 (en) * 2020-12-10 2022-06-16 Agilent Technologies, Inc. Methods for sequencing polynucleotide fragments from both ends
CN116685850A (zh) 2020-12-15 2023-09-01 贝克顿迪金森公司 单细胞分泌组分析
KR20230121076A (ko) 2020-12-15 2023-08-17 제노다이브 파마 가부시키가이샤 Dna 샘플의 시퀀스에 있어서의 어댑터 결합 효율을평가하는 방법
CN112687339B (zh) * 2021-01-21 2021-12-14 深圳吉因加医学检验实验室 一种统计血浆dna片段测序数据中序列错误的方法和装置
JP2024513187A (ja) 2021-03-29 2024-03-22 イルミナ インコーポレイテッド ライブラリー中のｄｎａ損傷を評価し、アンプリコンサイズバイアスを正規化するための組成物及び方法
MX2023010495A (es) 2021-03-30 2023-09-18 Illumina Inc Metodos mejorados de preparacion de genoteca y adn complementarios isotermicos.
CA3211172A1 (en) 2021-03-31 2022-10-06 Illumina, Inc. Methods of preparing directional tagmentation sequencing libraries using transposon-based technology with unique molecular identifiers for error correction
US20220392575A1 (en) * 2021-05-19 2022-12-08 Illumina, Inc. Umi collapsing
KR20250019610A (ko) 2022-03-21 2025-02-10 빌리언투원, 인크. 치료 모니터링을 위한 메틸화된 세포 유리 dna의 분자 계수
US20250297406A1 (en) * 2022-04-18 2025-09-25 Chengdu Boe Optoelectronics Technology Co., Ltd. Adapters, adapter ligation reagent, kit, method for constructing dna library and method for sequencing gene
WO2024015962A1 (en) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Blocked asymmetric hairpin adaptors
WO2024124567A1 (zh) * 2022-12-16 2024-06-20 深圳华大生命科学研究院 纳米孔测序方法和试剂盒
CN118581200A (zh) * 2023-03-01 2024-09-03 体学生物科技股份有限公司 核酸分子的检测方法、检测套组及检测卡匣
WO2024216159A1 (en) 2023-04-13 2024-10-17 Esbiolab Llc Methods and compositions for nucleic acid sequencing using labeled nucleotides
WO2024216163A1 (en) 2023-04-13 2024-10-17 Esbiolab Llc Methods and compositions for nucleic acid sequencing using predominantly unlabeled nucleotides
CN116913403B (zh) * 2023-06-29 2026-04-28 中移(成都)信息通信科技有限公司 数据预处理方法、装置、电子设备及存储介质
WO2025010160A1 (en) 2023-07-06 2025-01-09 Pacific Biosciences Of California, Inc. Methods and compositions for stabilizing concatemers
WO2025117738A1 (en) * 2023-11-28 2025-06-05 Illumina, Inc. Methods of improving unique molecular index ligation efficiency
WO2025132779A2 (en) * 2023-12-22 2025-06-26 F. Hoffmann-La Roche Ag Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion
GB202400129D0 (en) 2024-01-04 2024-02-21 Cambridge Entpr Ltd Nucleic acid barcodes
WO2025235623A1 (en) 2024-05-08 2025-11-13 Esbiolab, Llc Methods and systems for nucleic acid sequencing using an error-buffered coding scheme
WO2026039088A2 (en) 2024-06-03 2026-02-19 Esbiolab, Llc Excitation and detection methods for analyzing biomolecules
WO2025255512A1 (en) 2024-06-07 2025-12-11 Juno Therapeutics, Inc. Grna sequencing compositions and methods

Family Cites Families (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
US6159736A (en) 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
ATE377093T1 (de) 2000-07-07 2007-11-15 Visigen Biotechnologies Inc Sequenzbestimmung in echtzeit
AU2002227156A1 (en) 2000-12-01 2002-06-11 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US20040018520A1 (en) 2002-04-22 2004-01-29 James Thompson Trans-splicing enzymatic nucleic acid mediated biopharmaceutical and protein
JP3630414B2 (ja) 2002-05-21 2005-03-16 インターナショナル・ビジネス・マシーンズ・コーポレーション 塩基配列のクラスタ生成システム、塩基配列のクラスタ生成方法、該クラスタ生成方法を実行するためのプログラム、および該プログラムを記憶したコンピュータ可読な記録媒体、および塩基配列情報提供システム
WO2004018497A2 (en) 2002-08-23 2004-03-04 Solexa Limited Modified nucleotides for polynucleotide sequencing
EP1701785A1 (en) 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
CA2579150C (en) 2004-09-17 2014-11-25 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
WO2006064199A1 (en) 2004-12-13 2006-06-22 Solexa Limited Improved method of nucleotide detection
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
CA2648149A1 (en) 2006-03-31 2007-11-01 Solexa, Inc. Systems and devices for sequence by synthesis analysis
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
EP2414548B1 (en) 2009-03-30 2015-10-21 Illumina, Inc. Gene expression analysis in single cells
EP2513333B1 (en) * 2009-12-17 2013-10-02 Keygene N.V. Restriction enzyme based whole genome sequencing
US9260745B2 (en) 2010-01-19 2016-02-16 Verinata Health, Inc. Detecting and classifying copy number variation
EP2370599B1 (en) * 2010-01-19 2015-01-21 Verinata Health, Inc Method for determining copy number variations
WO2011100617A2 (en) 2010-02-12 2011-08-18 Life Technologies Corporation Nucleic acid, biomolecule and polymer identifier codes
US20120053845A1 (en) 2010-04-27 2012-03-01 Jeremy Bruestle Method and system for analysis and error correction of biological sequences and inference of relationship for multiple samples
JP5516880B2 (ja) 2010-07-09 2014-06-11 独立行政法人産業技術総合研究所 配列解析装置、配列解析方法およびコンピュータプログラム
ES2523140T3 (es) 2010-09-21 2014-11-21 Population Genetics Technologies Ltd. Aumento de la confianza en las identificaciones de alelos con el recuento molecular
EP2619329B1 (en) 2010-09-24 2019-05-22 The Board of Trustees of The Leland Stanford Junior University Direct capture, amplification and sequencing of target dna using immobilized primers
ES2986436T3 (es) * 2011-04-15 2024-11-11 Univ Johns Hopkins Sistema de secuenciación segura
EP3395957B1 (en) * 2011-04-25 2020-08-12 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
DK2729580T3 (en) * 2011-07-08 2015-12-14 Keygene Nv SEQUENCE BASED genotyping BASED ON OLIGONUKLEOTIDLIGERINGSASSAYS
AU2011373694A1 (en) * 2011-07-26 2013-05-02 Verinata Health, Inc. Method for determining the presence or absence of different aneuploidies in a sample
US20150087537A1 (en) 2011-08-31 2015-03-26 Life Technologies Corporation Methods, Systems, Computer Readable Media, and Kits for Sample Identification
US9845552B2 (en) 2011-10-27 2017-12-19 Verinata Health, Inc. Set membership testers for aligning nucleic acid samples
US20160153039A1 (en) 2012-01-26 2016-06-02 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
US20130267428A1 (en) 2012-02-10 2013-10-10 Washington University In St. Louis High throughput digital karyotyping for biome characterization
WO2013138510A1 (en) 2012-03-13 2013-09-19 Patel Abhijit Ajit Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
PT2828218T (pt) 2012-03-20 2020-11-11 Univ Washington Through Its Center For Commercialization Métodos para baixar a taxa de erro da sequenciação paralela massiva de adn utilizando sequenciação duplex de consensus
PT2850211T (pt) 2012-05-14 2021-11-29 Irepertoire Inc Método para aumentar a precisão na deteção quantitativa de polinucleótidos
EP3937179A1 (en) 2012-05-21 2022-01-12 Distributed Bio Inc Epitope focusing by variable effective antigen surface concentration
AU2013267609C1 (en) 2012-05-31 2019-01-03 Board Of Regents, The University Of Texas System Method for accurate sequencing of DNA
US10777301B2 (en) 2012-07-13 2020-09-15 Pacific Biosciences For California, Inc. Hierarchical genome assembly method using single long insert library
US20140024541A1 (en) 2012-07-17 2014-01-23 Counsyl, Inc. Methods and compositions for high-throughput sequencing
DK2970951T3 (da) 2013-03-13 2019-05-13 Illumina Inc Fremgangsmåder til nukleinsyresekventering
WO2014151117A1 (en) 2013-03-15 2014-09-25 The Board Of Trustees Of The Leland Stanford Junior University Identification and use of circulating nucleic acid tumor markers
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
WO2014201273A1 (en) 2013-06-12 2014-12-18 The Broad Institute, Inc. High-throughput rna-seq
CN105593683B (zh) 2013-10-01 2018-11-30 考利达基因组股份有限公司 鉴定基因组中的变异的定相和连接方法
EP3058091B1 (en) * 2013-10-18 2020-03-25 The Broad Institute, Inc. Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing
ES2660989T3 (es) 2013-12-28 2018-03-27 Guardant Health, Inc. Métodos y sistemas para detectar variantes genéticas
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
WO2015179493A1 (en) 2014-05-23 2015-11-26 Centrillion Technology Holding Corporation Methods for generating and decoding barcodes
EP4328322A3 (en) 2014-07-30 2024-05-22 President and Fellows of Harvard College Probe library construction
CN107075730A (zh) 2014-09-12 2017-08-18 利兰·斯坦福青年大学托管委员会 循环核酸的鉴定及用途
US11661597B2 (en) 2015-04-15 2023-05-30 The Board Of Trustees Of The Leland Stanford Junior University Robust quantification of single molecules in next-generation sequencing using non-random combinatorial oligonucleotide barcodes
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
US20160370380A1 (en) 2015-06-16 2016-12-22 Pharmaseq, Inc. Combinatorial antibody diagnostic
CN105063208B (zh) 2015-08-10 2018-03-06 北京吉因加科技有限公司 一种血浆中游离的目标dna低频突变富集测序方法
CA3006792A1 (en) 2015-12-08 2017-06-15 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
US11708574B2 (en) 2016-06-10 2023-07-25 Myriad Women's Health, Inc. Nucleic acid sequencing adapters and uses thereof
US10676736B2 (en) 2016-08-05 2020-06-09 Bio-Rad Laboratories, Inc. Second strand direct
SG11201906428SA (en) 2017-01-18 2019-08-27 Illumina Inc Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
WO2018148289A2 (en) 2017-02-08 2018-08-16 Integrated Dna Technologies, Inc. Duplex adapters and duplex sequencing
IL269431B2 (en) 2017-03-23 2025-05-01 Univ Washington Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing
AU2018261332B2 (en) 2017-05-01 2024-12-05 Illumina, Inc. Optimal index sequences for multiplex massively parallel sequencing
EP3622089B1 (en) 2017-05-08 2024-07-17 Illumina, Inc. Method for sequencing using universal short adapters for indexing of polynucleotide samples
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
AU2018366213B2 (en) 2017-11-08 2025-05-15 Twinstrand Biosciences, Inc. Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
BR112020016516A2 (pt) 2018-02-13 2020-12-15 Twinstrand Biosciences, Inc. Métodos e reagentes para detectar e avaliar a genotoxicidade
CA3093846A1 (en) 2018-03-15 2019-09-19 Twinstrand Biosciences, Inc. Methods and reagents for enrichment of nucleic acid material for sequencing applications and other nucleic acid material interrogations
WO2019222560A1 (en) 2018-05-16 2019-11-21 Twinstrand Biosciences, Inc. Methods and reagents for resolving nucleic acid mixtures and mixed cell populations and associated applications
BR112021000409A2 (pt) 2018-07-12 2021-04-06 Twinstrand Biosciences, Inc. Métodos e reagentes para caracterizar edição genômica, expansão clonal e aplicações associadas
JP7541363B2 (ja) 2018-10-16 2024-08-28 ツインストランド・バイオサイエンシズ・インコーポレイテッド プーリングを介した多数の試料の効率的な遺伝子型決定のための方法および試薬
WO2021022237A1 (en) 2019-08-01 2021-02-04 Twinstrand Biosciences, Inc. Methods and reagents for nucleic acid sequencing and associated applications

Also Published As

Publication number Publication date
EP3736341A1 (en) 2020-11-11
ZA201707231B (en) 2021-02-24
CN108138227B (zh) 2021-09-17
WO2016176091A8 (en) 2017-12-21
RU2017137401A3 (es) 2019-05-28
IL255187B (en) 2021-10-31
FI3289097T4 (fi) 2023-12-01
CN113832139A (zh) 2021-12-24
KR102091312B1 (ko) 2020-03-19
EP4266314C0 (en) 2025-12-24
IL294600B2 (en) 2024-05-01
AU2022200179A1 (en) 2022-02-10
US20160319345A1 (en) 2016-11-03
US10844428B2 (en) 2020-11-24
US12577617B2 (en) 2026-03-17
EP4266314A3 (en) 2024-01-24
IL294600B1 (en) 2024-01-01
CA2983935C (en) 2021-04-20
RU2017137401A (ru) 2019-05-28
EP4266314B1 (en) 2025-12-24
WO2016176091A1 (en) 2016-11-03
EP4266314A2 (en) 2023-10-25
CA3109403A1 (en) 2016-11-03
ES2961338T3 (es) 2024-03-11
DK3736341T3 (da) 2023-09-18
EP4700778A2 (en) 2026-02-25
DK3289097T4 (da) 2023-12-04
CN108138227A (zh) 2018-06-08
AU2019250200A1 (en) 2019-11-07
SG10202006185QA (en) 2020-07-29
IL285319B (en) 2022-10-01
IL294600A (en) 2022-09-01
MX2023004394A (es) 2023-05-04
AU2016256351B2 (en) 2019-07-18
AU2016256351A1 (en) 2017-11-09
HK1244513A1 (en) 2018-08-10
US11866777B2 (en) 2024-01-09
AU2022200179B2 (en) 2025-05-22
ES2799074T5 (es) 2024-02-26
EP3289097B1 (en) 2020-03-18
RU2704286C2 (ru) 2019-10-25
US20240084376A1 (en) 2024-03-14
JP6685324B2 (ja) 2020-04-22
EP3736341B1 (en) 2023-08-23
IL255187A0 (en) 2017-12-31
FI3736341T3 (fi) 2023-09-21
JP2018514207A (ja) 2018-06-07
CA2983935A1 (en) 2016-11-03
BR112017024118A2 (pt) 2018-07-31
US20210108262A1 (en) 2021-04-15
SG11201708859XA (en) 2017-11-29
MX2017013775A (es) 2018-08-15
NZ736609A (en) 2020-06-26
KR20180020137A (ko) 2018-02-27
EP3289097B2 (en) 2023-08-30
EP3289097A1 (en) 2018-03-07
IL285319A (en) 2021-09-30
DK3289097T3 (da) 2020-06-02
ES2799074T3 (es) 2020-12-14
MY181983A (en) 2021-01-18
ES3063683T3 (en) 2026-04-20
IL285319B2 (en) 2023-02-01
DK3736341T5 (da) 2024-08-26
AU2019250200B2 (en) 2021-10-14

Similar Documents

Publication Publication Date Title
MX2023004394A (es) Supresion de error en fragmentos de adn secuenciados usando lecturas redundantes con indices moleculares unicos (imu).
MY188348A (en) Single-molecule sequencing of plasma dna
WO2014209597A3 (en) Massively parallel sequencing of random dna fragments for determination of fetal fraction
MX2017010734A (es) Terapia de combinacion con factores de coagulacion y anticuepos multiespecificos.
BR112016016916A2 (pt) método de tratamento de angiodema hereditário (hae)
GB201216076D0 (en) Methods for obtaining a sequence
AU2015286820A8 (en) Stabilization of poly(A) sequence encoding DNA sequences
EP4604127A3 (en) Methods and processes for non-invasive assessment of genetic variations
EP3376997A4 (en) Labeled nucleotide analogues, reaction mixtures and methods and systems for the sequencing
EP4272660A3 (en) Embolectomy system
FI20155280A7 (fi) Aivovamman prognostisia ja diagnostisia glykaanipohjaisia biomarkkereita
HK1255446A1 (zh) 分子质量保证方法在测序中的应用
EP4461821A3 (en) Polymerase compositions and methods of making and using same
MX2022000289A (es) Metodos de evaluacion genomica en ganado.
EP3272081A4 (en) Method and system for establishing and managing multi-domain virtual topology (mdvt)
GB201516975D0 (en) PARK2 as epigenetic marker for the identification of immune cells, in particular monocytes
Rodrigo On fractional matrix exponentials and their explicit calculation
Chen et al. Asymptotic expansions for the constants of Landau and Lebesgue
WO2016176440A3 (en) Thrombin-thrombomodulin fusion proteins as protein c activators
EP3266161A4 (en) Method and system for establishing and managing multi-domain virtual tunnel (mvt)
Magne et al. Supplementary Material for: Somatic Mutations Are Not Observed by Exome Sequencing of Lymphocyte DNA from Monozygotic Twins Discordant for Congenital Hypothyroidism due to Thyroid Dysgenesis
DU et al. The Correlation between Angiotensinogen Gene M235T Polymorphism and Hypertension Combined with Depression
Satyavani Frequency Estimation of Prothrombin Allelic Polymorphisms in Indian Population
XU et al. The expressions of miR-196b and its prognostic significances in pediatric acute myeloid leukemia
LI et al. Effects of temperature and time on the quality of cryoprecipitate