MX336728B - Metodo de purificacion para proteinas de union de cation divalente en resina de intercambio anionico. - Google Patents

Metodo de purificacion para proteinas de union de cation divalente en resina de intercambio anionico.

Info

Publication number
MX336728B
MX336728B MX2012012621A MX2012012621A MX336728B MX 336728 B MX336728 B MX 336728B MX 2012012621 A MX2012012621 A MX 2012012621A MX 2012012621 A MX2012012621 A MX 2012012621A MX 336728 B MX336728 B MX 336728B
Authority
MX
Mexico
Prior art keywords
binding proteins
divalent cation
exchange resin
anion exchange
cation binding
Prior art date
Application number
MX2012012621A
Other languages
English (en)
Other versions
MX2012012621A (es
Inventor
Artur Mitterer
Meinhard Hasslacher
Christian Fiedler
Original Assignee
Baxter Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Int filed Critical Baxter Int
Publication of MX2012012621A publication Critical patent/MX2012012621A/es
Publication of MX336728B publication Critical patent/MX336728B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4721—Lipocortins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/647—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21021—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Hematology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Diabetes (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Manufacture Of Macromolecular Shaped Articles (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere a un método para la purificación de proteínas de unión de catión divalente con alta pureza en un material de resma de intercambio aniónico, a proteínas de unión de catión divalente que se pueden obtener a través de dicho método, y a un equipo que comprende medios para realizar dicho método.
MX2012012621A 2010-04-29 2011-04-29 Metodo de purificacion para proteinas de union de cation divalente en resina de intercambio anionico. MX336728B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32948710P 2010-04-29 2010-04-29
PCT/EP2011/056832 WO2011135071A1 (en) 2010-04-29 2011-04-29 Purification method for divalent cation binding proteins on anion exchange resin

Publications (2)

Publication Number Publication Date
MX2012012621A MX2012012621A (es) 2013-05-06
MX336728B true MX336728B (es) 2016-01-27

Family

ID=44625749

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012012621A MX336728B (es) 2010-04-29 2011-04-29 Metodo de purificacion para proteinas de union de cation divalente en resina de intercambio anionico.

Country Status (16)

Country Link
US (1) US10202416B2 (es)
EP (1) EP2563805B8 (es)
JP (1) JP5883849B2 (es)
KR (1) KR101780518B1 (es)
CN (2) CN106967150B (es)
AU (1) AU2011247567B2 (es)
BR (1) BR112012027793B1 (es)
CA (1) CA2797614A1 (es)
DK (1) DK2563805T3 (es)
ES (1) ES2790884T3 (es)
MX (1) MX336728B (es)
NZ (1) NZ603289A (es)
PL (1) PL2563805T3 (es)
PT (1) PT2563805T (es)
SG (1) SG185005A1 (es)
WO (1) WO2011135071A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG185005A1 (en) 2010-04-29 2012-11-29 Baxter Int Purification method for divalent cation binding proteins on anion exchange resin
AU2012322948B2 (en) * 2011-10-14 2014-11-06 Takeda Pharmaceutical Company Limited Protein purification by anion exchange chromatography
ES2700933T3 (es) 2011-10-14 2019-02-20 Baxalta GmbH Purificación de proteínas mediante cromatografía de intercambio aniónico
CN103509082B (zh) * 2012-06-15 2016-03-02 郭怀祖 一种糖基化蛋白和非糖基化蛋白的分离方法
NZ629845A (en) 2013-03-15 2016-10-28 Baxalta Inc Purification method for vitamin k dependent proteins by anion exchange chromatography
GB2542391A (en) 2015-09-17 2017-03-22 Annexin Pharmaceuticals Ab Process of manufacture
GB2552724B (en) * 2015-09-17 2020-05-06 Annexin Pharmaceuticals Ab Purification of Annexin A5 by anion exchange and affinity chromatography
GB2552853B (en) * 2015-09-17 2018-11-21 Annexin Pharmaceuticals Ab Purification of Annexin A5 from a composition that includes a calcium ion chelator
WO2021002275A1 (ja) * 2019-07-02 2021-01-07 日本メジフィジックス株式会社 ２２６Ｒａ含有溶液の精製方法、２２６Ｒａターゲットの製造方法および２２５Ａｃの製造方法
IL322843A (en) * 2023-03-14 2025-10-01 Amgen Inc Anion exchange chromatography processes using a primary amine ligand

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GB8318754D0 (en) * 1983-07-11 1983-08-10 Fagerhol M K F Human proteins anti-sera test kits
US4981952A (en) 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
CA2143125C (en) 1992-08-27 2008-09-23 Koenraad Mertens Antibodies specific for a haemostatic protein, their use for isolating intact protein, haemostatic compositions devoid of proteolytic cleavage products of the protein
DE4342132C1 (de) * 1993-12-10 1994-11-03 Octapharma Ag Verfahren zur Herstellung virusinaktivierte Vitamin K abhängige Plasmakomponenten sowie Protein C und Protein S enthaltender Mittel durch Membran-Chromatographie
DE4406515C1 (de) 1994-02-28 1995-10-19 Immuno Ag Verfahren zur Isolierung und Reinigung Vitamin K-abhängiger Proteine
US5714583A (en) 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods
JPH10150980A (ja) * 1996-11-20 1998-06-09 Chemo Sero Therapeut Res Inst プロトロンビンの精製方法
US6344596B1 (en) 1997-02-14 2002-02-05 American Red Cross Expression of active human factor IX in mammary tissue and of milk non human transgenic mammals
GB9902000D0 (en) * 1999-01-30 1999-03-17 Delta Biotechnology Ltd Process
KR100841599B1 (ko) 2000-07-21 2008-06-26 자이단호진 가가쿠오요비겟세이료호겐쿠쇼 칼슘 이온-결합 단백질의 정제 방법
US20040106779A1 (en) * 2002-12-03 2004-06-03 Bigler Douglas E. Modified factor IX preparation
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EP2360171A1 (en) * 2004-12-23 2011-08-24 Novo Nordisk Health Care AG Reduction of the content of protein contaminants in compositions comprising a vitamin K-dependent protein of interest
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FR2901796A1 (fr) 2006-05-31 2007-12-07 Lab Francais Du Fractionnement Procede d'extraction d'une ou de plusieurs proteines presentes dans du lait
FR2901707B1 (fr) 2006-05-31 2017-09-29 Lab Francais Du Fractionnement Composition de facteur vii recombinant ou transgenique, chaque molecule de facteur vii possedant deux sites de n-glycosylation a motifs glycanniques definis
DK2125866T3 (da) * 2007-02-28 2013-07-29 Baxter Int Fremgangsmåde til rensning af rekombinant blodkoagulationsfaktor IX, som er beriget med sulfaterede og/eller phosphorylerede molekyler
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SG185005A1 (en) 2010-04-29 2012-11-29 Baxter Int Purification method for divalent cation binding proteins on anion exchange resin
ES2700933T3 (es) 2011-10-14 2019-02-20 Baxalta GmbH Purificación de proteínas mediante cromatografía de intercambio aniónico
AU2012322948B2 (en) 2011-10-14 2014-11-06 Takeda Pharmaceutical Company Limited Protein purification by anion exchange chromatography

Also Published As

Publication number Publication date
BR112012027793B1 (pt) 2022-10-11
EP2563805B8 (en) 2020-04-15
EP2563805A1 (en) 2013-03-06
BR112012027793A8 (pt) 2018-06-05
EP2563805B1 (en) 2020-02-26
WO2011135071A1 (en) 2011-11-03
MX2012012621A (es) 2013-05-06
SG185005A1 (en) 2012-11-29
JP5883849B2 (ja) 2016-03-15
KR101780518B1 (ko) 2017-09-21
PT2563805T (pt) 2020-05-18
DK2563805T3 (da) 2020-05-18
CN106967150B (zh) 2020-12-04
AU2011247567B2 (en) 2014-06-19
US10202416B2 (en) 2019-02-12
ES2790884T3 (es) 2020-10-29
CN106967150A (zh) 2017-07-21
KR20130062938A (ko) 2013-06-13
BR112012027793A2 (pt) 2016-08-02
CN103025757A (zh) 2013-04-03
NZ603289A (en) 2014-07-25
JP2013525411A (ja) 2013-06-20
CA2797614A1 (en) 2011-11-03
PL2563805T3 (pl) 2020-08-10
US20120108513A1 (en) 2012-05-03

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