MX364532B - Celulas de hongos filamentosos deficientes de o-manosiltransferasa y metodos de uso de las mismas. - Google Patents

Celulas de hongos filamentosos deficientes de o-manosiltransferasa y metodos de uso de las mismas.

Info

Publication number
MX364532B
MX364532B MX2016000128A MX2016000128A MX364532B MX 364532 B MX364532 B MX 364532B MX 2016000128 A MX2016000128 A MX 2016000128A MX 2016000128 A MX2016000128 A MX 2016000128A MX 364532 B MX364532 B MX 364532B
Authority
MX
Mexico
Prior art keywords
filamentous fungal
mutation
mannosyltransferase
fungal cell
methods
Prior art date
Application number
MX2016000128A
Other languages
English (en)
Other versions
MX2016000128A (es
Inventor
Markku Saloheimo
Jari Natunen
Jukka Hiltunen
Anne Huuskonen
Ostermeier Christian
Patrick Sommer Benjamin
Wahl Ramon
Original Assignee
Glykos Finland Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=48740957&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX364532(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Glykos Finland Oy filed Critical Glykos Finland Oy
Publication of MX2016000128A publication Critical patent/MX2016000128A/es
Publication of MX364532B publication Critical patent/MX364532B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01109—Dolichyl-phosphate-mannose-protein mannosyltransferase (2.4.1.109)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)

Abstract

La presente invención se refiere a una célula de hongo filamentoso deficiente de PMT, caracterizada porque comprende a) una primera mutación en un gen que codifica una proteasa endógena que reduce o elimina una actividad de proteasa endógena en comparación con una célula de hongo filamentoso parental que no tiene dicha primera mutación, dicha proteasa endógena se selecciona de entre proteasas aspárticas, proteasas de serina similar a tripsina, proteasas de subtilisina, proteasas glutámicas y proteasas de sedolisin, y b) una segunda mutación en un gen PMT que reduce la actividad endógena de O-manosiltransferasa en comparación con una célula de hongo filamentoso parental que no tiene dicha segunda mutación, en donde dicha célula de hongo filamentoso se selecciona del grupo que consiste de célula de Trichoderma, Neurospora, Myceliophthora y Chrysosporium.
MX2016000128A 2013-07-04 2014-07-03 Celulas de hongos filamentosos deficientes de o-manosiltransferasa y metodos de uso de las mismas. MX364532B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13175141 2013-07-04
PCT/EP2014/064248 WO2015001049A1 (en) 2013-07-04 2014-07-03 O-mannosyltransferase deficient filamentous fungal cells and methods of use thereof

Publications (2)

Publication Number Publication Date
MX2016000128A MX2016000128A (es) 2016-03-01
MX364532B true MX364532B (es) 2019-04-29

Family

ID=48740957

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016000128A MX364532B (es) 2013-07-04 2014-07-03 Celulas de hongos filamentosos deficientes de o-manosiltransferasa y metodos de uso de las mismas.

Country Status (15)

Country Link
US (4) US20160376570A1 (es)
EP (1) EP3016970B1 (es)
KR (1) KR102111078B1 (es)
CN (1) CN105492457A (es)
BR (1) BR112015032695A2 (es)
CA (1) CA2916594C (es)
DK (1) DK3016970T3 (es)
ES (1) ES2739280T3 (es)
HR (1) HRP20191069T1 (es)
MX (1) MX364532B (es)
PL (1) PL3016970T3 (es)
RU (1) RU2016103373A (es)
SG (1) SG11201510814YA (es)
SI (1) SI3016970T1 (es)
WO (1) WO2015001049A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015293949B2 (en) 2014-07-21 2019-07-25 Teknologian Tutkimuskeskus Vtt Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
CA3113661A1 (en) * 2018-09-20 2020-03-26 Perfect Day, Inc. Methods and compositions for producing homokaryotic filamentous fungal cells
WO2020112881A1 (en) * 2018-11-28 2020-06-04 Novozymes A/S Modified filamentous fungal host cells
CN110885810B (zh) * 2019-12-16 2021-03-23 华中农业大学 一种利用真核发酵制备得到的抑菌蛋白酶CmYC1及其应用
CN115976033B (zh) * 2022-12-14 2024-05-17 西南大学 瓜实蝇tssk1和tssk3基因及其应用
KR20250004526A (ko) * 2023-06-29 2025-01-08 씨제이제일제당 (주) 아스파르트산 프로테아제 변이체
CN119931997B (zh) * 2025-04-09 2025-07-25 安琪酵母股份有限公司 天冬氨酸蛋白酶突变体及其应用

Family Cites Families (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4743546A (en) 1985-02-13 1988-05-10 Biotechnica International, Inc. Controlled gene excision
GB2276169A (en) 1990-07-05 1994-09-21 Celltech Ltd Antibodies specific for carcinoembryonic antigen
DE4244915C2 (de) 1992-08-14 1998-12-03 Widmar Prof Dr Tanner DNA-Moleküle codierend Proteine, die an der O-Glykosylierung von Proteinen beteiligt sind
EP0966521A1 (en) 1996-09-13 1999-12-29 Novo Nordisk Biotech, Inc. Cells having dna insertion mutations which produce altered amounts of a polypeptide
DE19754622A1 (de) 1997-12-09 1999-06-10 Antje Von Dr Schaewen Pflanzliche GntI-Sequenzen und Verwendung derselben zur Gewinnung von Pflanzen mit verminderter oder fehlender N-Acetylglucosaminyltransferase I (GnTI)-Aktivität
GB9902000D0 (en) 1999-01-30 1999-03-17 Delta Biotechnology Ltd Process
GB0021421D0 (en) 2000-08-31 2000-10-18 Oxford Glycosciences Uk Ltd Compounds
CN1592786A (zh) 2000-12-05 2005-03-09 宾夕法尼亚州立研究基金会 在酵母菌中高效生产异源性蛋白质的方法和组合物
PT1573017E (pt) 2002-12-20 2008-10-16 Unilever Nv Preparação de uma proteína anticongelante
CA2513213C (en) 2003-01-22 2013-07-30 Human Genome Sciences, Inc. Albumin fusion proteins
ZA200605449B (en) 2003-12-03 2007-11-28 Delta Biotechnology Ltd Interleukin-11 fusion proteins
JP5010923B2 (ja) 2004-02-09 2012-08-29 ヒューマン ジノーム サイエンシーズ， インコーポレイテッド アルブミン融合蛋白質
WO2006036817A2 (en) 2004-09-24 2006-04-06 Microbia, Inc. Fungal variants and uses thereof
CN101292024B (zh) * 2005-10-17 2012-04-18 诺维信公司 用于表达抗体的真菌突变体的用途
JP5284789B2 (ja) 2005-11-15 2013-09-11 グライコフィ， インコーポレイテッド Ｏ−グリコシル化を減少している糖タンパク質の生成
ES2553162T3 (es) 2006-05-16 2015-12-04 Daiichi Sankyo Company, Limited Método de producción de alta secreción de proteínas
US8680252B2 (en) 2006-12-10 2014-03-25 Dyadic International (Usa), Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
GB0711424D0 (en) 2007-06-13 2007-07-25 Novozymes Delta Ltd Recombinant transferrin mutants
CA2695830A1 (en) 2007-08-08 2009-02-12 Novozymes Biopharma Dk A/S Transferrin variants and conjugates
JP2011514157A (ja) 2008-02-20 2011-05-06 グライコフィ， インコーポレイテッド 蛋白質生産用ベクター及び酵母株
US20090253173A1 (en) * 2008-04-08 2009-10-08 Danisco Us Inc., Genencor Division Filamentous fungi with inactivated protease genes for altered protein production
EP2300446B1 (en) 2008-05-20 2011-12-07 Merck Sharp & Dohme Corp. Efficient production of heterologous proteins using mannosyl transferase inhibitors
EP2324121B1 (en) 2008-08-12 2012-11-07 GlycoFi, Inc. Improved vectors and yeast strains for protein production: ca2+ atpase overexpression
WO2010034708A1 (en) 2008-09-23 2010-04-01 Unilever Plc A polynucleotide expression cassette
WO2010092135A2 (en) 2009-02-11 2010-08-19 Novozymes Biopharma Uk Ltd. Albumin variants and conjugates
US20120059155A1 (en) 2009-05-07 2012-03-08 Novozymes Biopharma Dk A/S Method of Controlling O-Linked Glycosylation of Antibodies
EP2427486B1 (en) 2009-05-07 2015-02-25 Novozymes Biopharma DK A/S Method for purifying albumin
US8440456B2 (en) 2009-05-22 2013-05-14 Vib, Vzw Nucleic acids of Pichia pastoris and use thereof for recombinant production of proteins
CN102648286A (zh) 2009-10-16 2012-08-22 默沙东公司 在巴斯德毕赤氏酵母中生产缺乏与针对宿主细胞抗原的抗体的可检测交叉结合活性的蛋白质的方法
MX350399B (es) 2009-12-18 2017-09-06 Novozymes Inc Metodos para producir polipeptidos en mutantes de trichoderma deficientes en proteasa.
WO2011099028A1 (en) 2010-02-10 2011-08-18 Biocon Limited Method of reducing glycosylation of proteins, processes and proteins thereof
KR101930961B1 (ko) 2010-02-24 2018-12-19 머크 샤프 앤드 돔 코포레이션 피키아 파스토리스에서 생산된 치료 당단백질 상의 ｎ-글리코실화 부위 점유를 증가시키는 방법
RU2604811C2 (ru) 2010-05-27 2016-12-10 Мерк Шарп Энд Домэ Корп. Способ получения антител с улучшенными свойствами
US20120135461A1 (en) 2010-07-30 2012-05-31 William James Cook Production of glycoproteins with reduced o-glycosylation comprising the use of an alpha-1,2-mannosidase
MX338560B (es) 2010-11-24 2016-04-20 Glykos Finland Oy Enzimas de fusion que tienen actividad de n-acetilglucosaminil transferasa.
JP5885191B2 (ja) 2011-01-04 2016-03-15 国立研究開発法人産業技術総合研究所 糖鎖改変酵母及びそれを用いた糖タンパク質の製造方法
KR20140114278A (ko) 2011-02-25 2014-09-26 머크 샤프 앤드 돔 코포레이션 변형된 ｏ-글리코실화를 갖는 단백질의 생산을 위한 효모 균주
JP2014517846A (ja) 2011-05-25 2014-07-24 メルク・シャープ・アンド・ドーム・コーポレーション 改善された特性を有するＦｃ含有ポリペプチドの製造方法
AR087433A1 (es) 2011-08-08 2014-03-26 Merck Sharp & Dohme Analogos de insulina n-glicosilados
EP2771477A4 (en) * 2011-10-27 2015-04-22 Merck Sharp & Dohme O-GLYCOSYLATION IN LOW EUKARYOTES
US20140286946A1 (en) 2011-10-31 2014-09-25 Merck Sharp & Dohme Corp. Method for preparing antibodies having improved properties
WO2013066765A1 (en) 2011-11-01 2013-05-10 Merck Sharp & Dohme Corp. Mutation of tup1 in glycoengineered yeast
EP2780463A4 (en) 2011-11-18 2015-07-01 Merck Sharp & Dohme FC-CONTAINING POLYPEPTIDES WITH INCREASED INFLAMMATORY FEATURES AND INCREASED FCRN BINDING
DK2852610T3 (en) * 2012-05-23 2018-09-03 Glykos Finland Oy PRODUCTION OF FUCOSYLED GLYCOPROTEIN
US20150275222A1 (en) 2012-10-22 2015-10-01 Bo Jiang Crz1 mutant fungal cells
CA2888645A1 (en) 2012-12-17 2014-06-26 Merck Sharp & Dohme Corp. Pmt2-, och1-, pmt5- mutant cells
US9518100B2 (en) 2013-02-13 2016-12-13 Merck Sharp & Dohme Corp. Methods for increasing N-glycan occupancy and reducing production of hybrid N-glycans in Pichia pastoris strains lacking Alg3 expression
CA2903739A1 (en) 2013-03-06 2014-09-12 Glaxosmithkline Llc Host cells and methods of use
EP2994483A4 (en) 2013-05-06 2017-02-08 Cell Machines, Inc. Methods and compositions related to large scale production of proteins
US20160115216A1 (en) 2013-06-07 2016-04-28 Novo Nordisk A/S Method for Making Mature Insulin Polypeptides
WO2015013116A1 (en) 2013-07-25 2015-01-29 Merck Sharp & Dohme Corp. Method for reducing the extent of o-mannosylation of glycoproteins
WO2015054039A1 (en) 2013-10-08 2015-04-16 Merck Sharp & Dohme Corp. Fc CONTAINING POLYPEPTIDES HAVING INCREASED BINDING TO FcGammaRIIB
WO2015073307A2 (en) 2013-11-13 2015-05-21 Merck Sharp & Dohme Corp. Fc CONTAINING POLYPEPTIDES HAVING INCREASED BINDING TO HUMAN DC-SIGN

Also Published As

Publication number Publication date
CN105492457A (zh) 2016-04-13
HRP20191069T1 (hr) 2019-11-01
EP3016970B1 (en) 2019-05-22
WO2015001049A1 (en) 2015-01-08
DK3016970T3 (da) 2019-06-24
US20200040314A1 (en) 2020-02-06
US20160376570A1 (en) 2016-12-29
PL3016970T3 (pl) 2019-09-30
SG11201510814YA (en) 2016-01-28
RU2016103373A3 (es) 2018-03-26
RU2016103373A (ru) 2017-08-10
CA2916594C (en) 2020-03-10
US20220213451A1 (en) 2022-07-07
KR20160030127A (ko) 2016-03-16
CA2916594A1 (en) 2015-01-08
US10724013B2 (en) 2020-07-28
BR112015032695A2 (pt) 2017-10-24
KR102111078B1 (ko) 2020-05-18
ES2739280T3 (es) 2020-01-30
SI3016970T1 (sl) 2019-08-30
EP3016970A1 (en) 2016-05-11
US20200362319A1 (en) 2020-11-19
MX2016000128A (es) 2016-03-01

Similar Documents

Publication Publication Date Title
MX2016000128A (es) Celulas de hongos filamentosos deficientes de o-manosiltransferasa y metodos de uso de las mismas.
MX2016000306A (es) Celulas de hongos filamentosos deficientes en multiples proteasas y métodos de uso de las mismas.
MX2014008272A (es) Celulas de hongos filamentosas deficientes de proteasa y metodos de uso de las mismas.
MX363788B (es) Métodos para la producción de polipéptidos procesados proteolíticamente.
HK1256737A1 (zh) 通过改变细胞表面信号和信号比选择性扩增不同的t细胞亚群
MX2017006127A (es) Metodos y composiciones para produccion de proteina de clara de huevo.
MX2016016903A (es) Animales no humanos que tienen un gen de muerte celular programada 1 humanizado.
WO2016057951A3 (en) Crispr oligonucleotides and gene editing
NZ769992A (en) Non-human animals having a humanized cluster of differentiation 47 gene
EP3957711A3 (en) Detergent composition comprising amylase and protease variants
WO2015200920A8 (en) Cultured mammalian limbal stem cells, methods for generating the same, and uses thereof
HUE071193T2 (hu) Sejtek transzfekció utáni korai izolálása (EPIC) biologikumok elõállításához
MX2017000083A (es) Compuestos de platino, composiciones y usos de estos.
MX2017004617A (es) Variantes estabilizadas de alfa amilasa y su uso.
MY180732A (en) Protein manufacture
EP4502167A3 (en) N-glycosylation
MX2016015893A (es) Produccion de fragmentos de fragmento cristalizable (fc).
MY182924A (en) Enzyme compositions and uses thereof
MX2015011781A (es) Medio de cultivo celular y metodos de produccion de anticuerpos.
TW201613974A (en) Tumor-selective CTLA-4 antagonists
MX363261B (es) Polipéptidos que tienen actividad de la fosfolipasa a y polinucleótidos que los codifican.
MX2020001223A (es) Proteinas de fusion tatk-cdkl5, composiciones, formulaciones y uso de estas.
MY197890A (en) Mutant strains of trichoderma reesei
WO2015175534A3 (en) Compositions and methods employing stem cell-derived cardiomyocytes
HK1255078A1 (zh) 细胞效能分析

Legal Events

Date Code Title Description
GB Transfer or rights

Owner name: GLYKOS FINLAND OY

FG Grant or registration