NL8100558A - Recombinant dna-techniek voor de bereiding van een op menselijk interferon lijkend eiwit; gen voor de expressie van een dergelijk eiwit; subeenheid van een dergelijk gen; werkwijze voor de bereiding van een dergelijk gen of een dergelijke subeenheid; plasmiderecombinant; werkwijze voor de bereiding daarvan; cel met daarin een dergelijk gen, subeenheid daarvan of plasmiderecombinant; werkwijze voor de bereiding van een dergelijke cel; werkwijze voor de bereiding van een eiwit; aldus bereid eiwit. - Google Patents

Recombinant dna-techniek voor de bereiding van een op menselijk interferon lijkend eiwit; gen voor de expressie van een dergelijk eiwit; subeenheid van een dergelijk gen; werkwijze voor de bereiding van een dergelijk gen of een dergelijke subeenheid; plasmiderecombinant; werkwijze voor de bereiding daarvan; cel met daarin een dergelijk gen, subeenheid daarvan of plasmiderecombinant; werkwijze voor de bereiding van een dergelijke cel; werkwijze voor de bereiding van een eiwit; aldus bereid eiwit. Download PDF

Info

Publication number: NL8100558A
Authority: NL; Netherlands
Prior art keywords: ctg; att; aac; ctc; cag
Prior art date: 1980-02-06

Application number

NL8100558A

Other languages

English (en)

Dutch (nl)

Original Assignee

Searle & Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1980-02-06

Filing date

1981-02-05

Publication date

1981-09-01

1981-02-05 Application filed by Searle & Co filed Critical Searle & Co

1981-09-01 Publication of NL8100558A publication Critical patent/NL8100558A/nl

Links

108010050904 Interferons Proteins 0.000 title claims description 149
108090000623 proteins and genes Proteins 0.000 title claims description 132
102000014150 Interferons Human genes 0.000 title claims description 88
229940079322 interferon Drugs 0.000 title claims description 70
238000000034 method Methods 0.000 title claims description 67
238000002360 preparation method Methods 0.000 title claims description 34
102000004169 proteins and genes Human genes 0.000 title claims description 25
108020004511 Recombinant DNA Proteins 0.000 title description 6
238000004519 manufacturing process Methods 0.000 title description 4
108020004414 DNA Proteins 0.000 claims description 50
239000013612 plasmid Substances 0.000 claims description 49
210000004027 cell Anatomy 0.000 claims description 45
108020004999 messenger RNA Proteins 0.000 claims description 40
239000002299 complementary DNA Substances 0.000 claims description 27
239000012634 fragment Substances 0.000 claims description 27
230000001580 bacterial effect Effects 0.000 claims description 19
102100034343 Integrase Human genes 0.000 claims description 18
108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 18
241000588724 Escherichia coli Species 0.000 claims description 16
210000002950 fibroblast Anatomy 0.000 claims description 16
238000013518 transcription Methods 0.000 claims description 14
230000035897 transcription Effects 0.000 claims description 14
238000011282 treatment Methods 0.000 claims description 13
230000002759 chromosomal effect Effects 0.000 claims description 12
102000004594 DNA Polymerase I Human genes 0.000 claims description 11
108010017826 DNA Polymerase I Proteins 0.000 claims description 11
108091033380 Coding strand Proteins 0.000 claims description 10
101710163270 Nuclease Proteins 0.000 claims description 10
239000000523 sample Substances 0.000 claims description 9
102000053602 DNA Human genes 0.000 claims description 8
238000002955 isolation Methods 0.000 claims description 8
239000013600 plasmid vector Substances 0.000 claims description 7
102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 claims description 6
108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 claims description 6
238000010367 cloning Methods 0.000 claims description 6
238000003780 insertion Methods 0.000 claims description 6
230000037431 insertion Effects 0.000 claims description 6
238000013519 translation Methods 0.000 claims description 5
238000011144 upstream manufacturing Methods 0.000 claims description 5
239000013611 chromosomal DNA Substances 0.000 claims description 4
230000000295 complement effect Effects 0.000 claims description 4
230000008569 process Effects 0.000 claims description 4
108091081024 Start codon Proteins 0.000 claims description 3
230000029087 digestion Effects 0.000 claims description 3
230000036961 partial effect Effects 0.000 claims description 3
238000010790 dilution Methods 0.000 claims description 2
239000012895 dilution Substances 0.000 claims description 2
239000011541 reaction mixture Substances 0.000 claims description 2
101100494773 Caenorhabditis elegans ctl-2 gene Proteins 0.000 claims 1
101100112369 Fasciola hepatica Cat-1 gene Proteins 0.000 claims 1
101100005271 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) cat-1 gene Proteins 0.000 claims 1
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 54
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 42
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 30
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 24
239000011780 sodium chloride Substances 0.000 description 21
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 20
239000000203 mixture Substances 0.000 description 20
108091034117 Oligonucleotide Proteins 0.000 description 19
239000000499 gel Substances 0.000 description 18
241000894006 Bacteria Species 0.000 description 17
230000000694 effects Effects 0.000 description 17
239000002773 nucleotide Substances 0.000 description 17
125000003729 nucleotide group Chemical group 0.000 description 17
239000000047 product Substances 0.000 description 17
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 15
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 14
108090000467 Interferon-beta Proteins 0.000 description 13
238000005119 centrifugation Methods 0.000 description 12
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 11
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 11
239000002253 acid Substances 0.000 description 11
238000000605 extraction Methods 0.000 description 11
238000011534 incubation Methods 0.000 description 11
YPHMISFOHDHNIV-FSZOTQKASA-N cycloheximide Chemical compound C1[C@@H](C)C[C@H](C)C(=O)[C@@H]1[C@H](O)CC1CC(=O)NC(=O)C1 YPHMISFOHDHNIV-FSZOTQKASA-N 0.000 description 10
DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 10
102000003996 Interferon-beta Human genes 0.000 description 9
240000004808 Saccharomyces cerevisiae Species 0.000 description 9
239000000284 extract Substances 0.000 description 9
108090000765 processed proteins & peptides Proteins 0.000 description 9
108020004705 Codon Proteins 0.000 description 8
229930006000 Sucrose Natural products 0.000 description 8
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 8
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 8
229920002401 polyacrylamide Polymers 0.000 description 8
229920001184 polypeptide Polymers 0.000 description 8
102000004196 processed proteins & peptides Human genes 0.000 description 8
239000005720 sucrose Substances 0.000 description 8
229920004890 Triton X-100 Polymers 0.000 description 7
239000013504 Triton X-100 Substances 0.000 description 7
150000007513 acids Chemical class 0.000 description 7
150000001413 amino acids Chemical class 0.000 description 7
SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 7
229940047124 interferons Drugs 0.000 description 7
239000008188 pellet Substances 0.000 description 7
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 7
229920005654 Sephadex Polymers 0.000 description 6
239000012507 Sephadex™ Substances 0.000 description 6
241000700605 Viruses Species 0.000 description 6
SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 6
230000000977 initiatory effect Effects 0.000 description 6
238000012163 sequencing technique Methods 0.000 description 6
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 5
102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 5
239000004098 Tetracycline Substances 0.000 description 5
229960000723 ampicillin Drugs 0.000 description 5
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 5
238000004458 analytical method Methods 0.000 description 5
230000002155 anti-virotic effect Effects 0.000 description 5
230000015572 biosynthetic process Effects 0.000 description 5
RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 5
NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 5
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 5
239000013613 expression plasmid Substances 0.000 description 5
238000000338 in vitro Methods 0.000 description 5
239000012071 phase Substances 0.000 description 5
239000002953 phosphate buffered saline Substances 0.000 description 5
230000002829 reductive effect Effects 0.000 description 5
238000003786 synthesis reaction Methods 0.000 description 5
229960002180 tetracycline Drugs 0.000 description 5
229930101283 tetracycline Natural products 0.000 description 5
235000019364 tetracycline Nutrition 0.000 description 5
150000003522 tetracyclines Chemical class 0.000 description 5
241000713838 Avian myeloblastosis virus Species 0.000 description 4
102000012410 DNA Ligases Human genes 0.000 description 4
108010061982 DNA Ligases Proteins 0.000 description 4
108091092195 Intron Proteins 0.000 description 4
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 4
QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 4
RJURFGZVJUQBHK-UHFFFAOYSA-N actinomycin D Natural products CC1OC(=O)C(C(C)C)N(C)C(=O)CN(C)C(=O)C2CCCN2C(=O)C(C(C)C)NC(=O)C1NC(=O)C1=C(N)C(=O)C(C)=C2OC(C(C)=CC=C3C(=O)NC4C(=O)NC(C(N5CCCC5C(=O)N(C)CC(=O)N(C)C(C(C)C)C(=O)OC4C)=O)C(C)C)=C3N=C21 RJURFGZVJUQBHK-UHFFFAOYSA-N 0.000 description 4
238000013459 approach Methods 0.000 description 4
239000008346 aqueous phase Substances 0.000 description 4
230000008901 benefit Effects 0.000 description 4
239000004202 carbamide Substances 0.000 description 4
HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 4
238000012869 ethanol precipitation Methods 0.000 description 4
UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 description 4
239000011654 magnesium acetate Substances 0.000 description 4
235000011285 magnesium acetate Nutrition 0.000 description 4
229940069446 magnesium acetate Drugs 0.000 description 4
239000002244 precipitate Substances 0.000 description 4
239000000243 solution Substances 0.000 description 4
PLVPPLCLBIEYEA-AATRIKPKSA-N (E)-3-(indol-3-yl)acrylic acid Chemical compound C1=CC=C2C(/C=C/C(=O)O)=CNC2=C1 PLVPPLCLBIEYEA-AATRIKPKSA-N 0.000 description 3
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 3
USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 3
239000005695 Ammonium acetate Substances 0.000 description 3
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
108091006905 Human Serum Albumin Proteins 0.000 description 3
102000008100 Human Serum Albumin Human genes 0.000 description 3
QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 3
-1 Montgomery Chemical compound 0.000 description 3
108091028043 Nucleic acid sequence Proteins 0.000 description 3
239000011543 agarose gel Substances 0.000 description 3
235000019257 ammonium acetate Nutrition 0.000 description 3
229940043376 ammonium acetate Drugs 0.000 description 3
239000002585 base Substances 0.000 description 3
239000000872 buffer Substances 0.000 description 3
229960003669 carbenicillin Drugs 0.000 description 3
238000006243 chemical reaction Methods 0.000 description 3
238000003776 cleavage reaction Methods 0.000 description 3
201000010099 disease Diseases 0.000 description 3
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 3
229960005542 ethidium bromide Drugs 0.000 description 3
239000000706 filtrate Substances 0.000 description 3
238000010438 heat treatment Methods 0.000 description 3
PLVPPLCLBIEYEA-UHFFFAOYSA-N indoleacrylic acid Natural products C1=CC=C2C(C=CC(=O)O)=CNC2=C1 PLVPPLCLBIEYEA-UHFFFAOYSA-N 0.000 description 3
230000006698 induction Effects 0.000 description 3
239000000463 material Substances 0.000 description 3
239000002609 medium Substances 0.000 description 3
238000012986 modification Methods 0.000 description 3
230000004048 modification Effects 0.000 description 3
238000012544 monitoring process Methods 0.000 description 3
230000007017 scission Effects 0.000 description 3
210000001519 tissue Anatomy 0.000 description 3
239000013598 vector Substances 0.000 description 3
210000003501 vero cell Anatomy 0.000 description 3
238000005406 washing Methods 0.000 description 3
108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
241000282552 Chlorocebus aethiops Species 0.000 description 2
108091026890 Coding region Proteins 0.000 description 2
108700010070 Codon Usage Proteins 0.000 description 2
108010092160 Dactinomycin Proteins 0.000 description 2
RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
241001646716 Escherichia coli K-12 Species 0.000 description 2
108010010803 Gelatin Proteins 0.000 description 2
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
108010047761 Interferon-alpha Proteins 0.000 description 2
CPLXHLVBOLITMK-UHFFFAOYSA-N Magnesium oxide Chemical compound [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 description 2
125000000729 N-terminal amino-acid group Chemical group 0.000 description 2
JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
101710141795 Ribonuclease inhibitor Proteins 0.000 description 2
229940122208 Ribonuclease inhibitor Drugs 0.000 description 2
102100037968 Ribonuclease inhibitor Human genes 0.000 description 2
MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 2
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
102000004357 Transferases Human genes 0.000 description 2
108090000992 Transferases Proteins 0.000 description 2
241000711975 Vesicular stomatitis virus Species 0.000 description 2
RJURFGZVJUQBHK-IIXSONLDSA-N actinomycin D Chemical compound C[C@H]1OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]2CCCN2C(=O)[C@@H](C(C)C)NC(=O)[C@H]1NC(=O)C1=C(N)C(=O)C(C)=C2OC(C(C)=CC=C3C(=O)N[C@@H]4C(=O)N[C@@H](C(N5CCC[C@H]5C(=O)N(C)CC(=O)N(C)[C@@H](C(C)C)C(=O)O[C@@H]4C)=O)C(C)C)=C3N=C21 RJURFGZVJUQBHK-IIXSONLDSA-N 0.000 description 2
UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 2
FPPNZSSZRUTDAP-UWFZAAFLSA-N carbenicillin Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)C(C(O)=O)C1=CC=CC=C1 FPPNZSSZRUTDAP-UWFZAAFLSA-N 0.000 description 2
239000001913 cellulose Substances 0.000 description 2
229920002678 cellulose Polymers 0.000 description 2
238000004587 chromatography analysis Methods 0.000 description 2
229960000640 dactinomycin Drugs 0.000 description 2
238000001962 electrophoresis Methods 0.000 description 2
210000003527 eukaryotic cell Anatomy 0.000 description 2
239000008273 gelatin Substances 0.000 description 2
229920000159 gelatin Polymers 0.000 description 2
235000019322 gelatine Nutrition 0.000 description 2
235000011852 gelatine desserts Nutrition 0.000 description 2
239000008103 glucose Substances 0.000 description 2
229960002897 heparin Drugs 0.000 description 2
229920000669 heparin Polymers 0.000 description 2
238000004128 high performance liquid chromatography Methods 0.000 description 2
PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
210000004185 liver Anatomy 0.000 description 2
229910001629 magnesium chloride Inorganic materials 0.000 description 2
229930182817 methionine Natural products 0.000 description 2
102000039446 nucleic acids Human genes 0.000 description 2
108020004707 nucleic acids Proteins 0.000 description 2
150000007523 nucleic acids Chemical class 0.000 description 2
229940046166 oligodeoxynucleotide Drugs 0.000 description 2
230000026731 phosphorylation Effects 0.000 description 2
238000006366 phosphorylation reaction Methods 0.000 description 2
230000001376 precipitating effect Effects 0.000 description 2
230000002285 radioactive effect Effects 0.000 description 2
230000009467 reduction Effects 0.000 description 2
238000011160 research Methods 0.000 description 2
108091008146 restriction endonucleases Proteins 0.000 description 2
238000010839 reverse transcription Methods 0.000 description 2
239000003161 ribonuclease inhibitor Substances 0.000 description 2
ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 2
238000010186 staining Methods 0.000 description 2
239000006228 supernatant Substances 0.000 description 2
RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
230000001131 transforming effect Effects 0.000 description 2
239000001226 triphosphate Substances 0.000 description 2
235000011178 triphosphate Nutrition 0.000 description 2
230000009385 viral infection Effects 0.000 description 2
210000005253 yeast cell Anatomy 0.000 description 2
MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
229920001817 Agar Polymers 0.000 description 1
CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 1
108091003079 Bovine Serum Albumin Proteins 0.000 description 1
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 1
102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 description 1
102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
101150013191 E gene Proteins 0.000 description 1
208000003468 Ehrlich Tumor Carcinoma Diseases 0.000 description 1
241000710188 Encephalomyocarditis virus Species 0.000 description 1
BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 description 1
PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 description 1
241000206602 Eukaryota Species 0.000 description 1
108091029865 Exogenous DNA Proteins 0.000 description 1
229920001917 Ficoll Polymers 0.000 description 1
102000003886 Glycoproteins Human genes 0.000 description 1
108090000288 Glycoproteins Proteins 0.000 description 1
101000635799 Homo sapiens Run domain Beclin-1-interacting and cysteine-rich domain-containing protein Proteins 0.000 description 1
108090000144 Human Proteins Proteins 0.000 description 1
102000003839 Human Proteins Human genes 0.000 description 1
102100024319 Intestinal-type alkaline phosphatase Human genes 0.000 description 1
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
239000006137 Luria-Bertani broth Substances 0.000 description 1
102000016943 Muramidase Human genes 0.000 description 1
108010014251 Muramidase Proteins 0.000 description 1
241001529936 Murinae Species 0.000 description 1
108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
206010028980 Neoplasm Diseases 0.000 description 1
239000000020 Nitrocellulose Substances 0.000 description 1
108020005187 Oligonucleotide Probes Proteins 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
108020002230 Pancreatic Ribonuclease Proteins 0.000 description 1
102000005891 Pancreatic ribonuclease Human genes 0.000 description 1
241000577979 Peromyscus spicilegus Species 0.000 description 1
239000004698 Polyethylene Substances 0.000 description 1
108091034057 RNA (poly(A)) Proteins 0.000 description 1
239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 1
102100030852 Run domain Beclin-1-interacting and cysteine-rich domain-containing protein Human genes 0.000 description 1
108020004682 Single-Stranded DNA Proteins 0.000 description 1
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
206010042618 Surgical procedure repeated Diseases 0.000 description 1
108020004440 Thymidine kinase Proteins 0.000 description 1
102000004142 Trypsin Human genes 0.000 description 1
108090000631 Trypsin Proteins 0.000 description 1
208000036142 Viral infection Diseases 0.000 description 1
229930003451 Vitamin B1 Natural products 0.000 description 1
230000001594 aberrant effect Effects 0.000 description 1
239000002250 absorbent Substances 0.000 description 1
230000002745 absorbent Effects 0.000 description 1
239000008272 agar Substances 0.000 description 1
239000003513 alkali Substances 0.000 description 1
230000004075 alteration Effects 0.000 description 1
229910021529 ammonia Inorganic materials 0.000 description 1
230000003466 anti-cipated effect Effects 0.000 description 1
239000003443 antiviral agent Substances 0.000 description 1
238000000211 autoradiogram Methods 0.000 description 1
238000000376 autoradiography Methods 0.000 description 1
238000007630 basic procedure Methods 0.000 description 1
SRSXLGNVWSONIS-UHFFFAOYSA-N benzenesulfonic acid Chemical compound OS(=O)(=O)C1=CC=CC=C1 SRSXLGNVWSONIS-UHFFFAOYSA-N 0.000 description 1
229940092714 benzenesulfonic acid Drugs 0.000 description 1
229940098773 bovine serum albumin Drugs 0.000 description 1
239000001110 calcium chloride Substances 0.000 description 1
235000011148 calcium chloride Nutrition 0.000 description 1
229910001628 calcium chloride Inorganic materials 0.000 description 1
244000309466 calf Species 0.000 description 1
201000011510 cancer Diseases 0.000 description 1
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
230000010261 cell growth Effects 0.000 description 1
239000007795 chemical reaction product Substances 0.000 description 1
238000010276 construction Methods 0.000 description 1
238000012937 correction Methods 0.000 description 1
230000016396 cytokine production Effects 0.000 description 1
230000009089 cytolysis Effects 0.000 description 1
230000000120 cytopathologic effect Effects 0.000 description 1
GYOZYWVXFNDGLU-XLPZGREQSA-N dTMP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)C1 GYOZYWVXFNDGLU-XLPZGREQSA-N 0.000 description 1
238000003936 denaturing gel electrophoresis Methods 0.000 description 1
238000001514 detection method Methods 0.000 description 1
239000003599 detergent Substances 0.000 description 1
238000010586 diagram Methods 0.000 description 1
239000003085 diluting agent Substances 0.000 description 1
238000006073 displacement reaction Methods 0.000 description 1
238000004090 dissolution Methods 0.000 description 1
229940079593 drug Drugs 0.000 description 1
239000003814 drug Substances 0.000 description 1
239000003937 drug carrier Substances 0.000 description 1
238000001035 drying Methods 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
210000003746 feather Anatomy 0.000 description 1
238000001914 filtration Methods 0.000 description 1
210000003953 foreskin Anatomy 0.000 description 1
238000001502 gel electrophoresis Methods 0.000 description 1
238000010353 genetic engineering Methods 0.000 description 1
239000011521 glass Substances 0.000 description 1
239000011491 glass wool Substances 0.000 description 1
238000000265 homogenisation Methods 0.000 description 1
210000005260 human cell Anatomy 0.000 description 1
238000009396 hybridization Methods 0.000 description 1
238000001727 in vivo Methods 0.000 description 1
208000015181 infectious disease Diseases 0.000 description 1
230000002401 inhibitory effect Effects 0.000 description 1
230000005764 inhibitory process Effects 0.000 description 1
238000005304 joining Methods 0.000 description 1
230000002147 killing effect Effects 0.000 description 1
125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
210000000265 leukocyte Anatomy 0.000 description 1
230000000670 limiting effect Effects 0.000 description 1
239000007788 liquid Substances 0.000 description 1
238000005567 liquid scintillation counting Methods 0.000 description 1
210000005265 lung cell Anatomy 0.000 description 1
210000004698 lymphocyte Anatomy 0.000 description 1
239000006166 lysate Substances 0.000 description 1
239000004325 lysozyme Substances 0.000 description 1
229960000274 lysozyme Drugs 0.000 description 1
235000010335 lysozyme Nutrition 0.000 description 1
239000000395 magnesium oxide Substances 0.000 description 1
210000001161 mammalian embryo Anatomy 0.000 description 1
125000001360 methionine group Chemical class N[C@@H](CCSC)C(=O)* 0.000 description 1
238000002156 mixing Methods 0.000 description 1
BAPROVDXKNPHAM-UHFFFAOYSA-N n-(2-aminoethyl)-3-(3,5-ditert-butyl-4-hydroxyphenyl)propanamide Chemical compound CC(C)(C)C1=CC(CCC(=O)NCCN)=CC(C(C)(C)C)=C1O BAPROVDXKNPHAM-UHFFFAOYSA-N 0.000 description 1
229920001220 nitrocellulos Polymers 0.000 description 1
229940124276 oligodeoxyribonucleotide Drugs 0.000 description 1
239000002751 oligonucleotide probe Substances 0.000 description 1
239000012188 paraffin wax Substances 0.000 description 1
210000001322 periplasm Anatomy 0.000 description 1
150000002989 phenols Chemical class 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
UEZVMMHDMIWARA-UHFFFAOYSA-M phosphonate Chemical group [O-]P(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-M 0.000 description 1
229920003023 plastic Polymers 0.000 description 1
239000004033 plastic Substances 0.000 description 1
229920000573 polyethylene Polymers 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
239000001267 polyvinylpyrrolidone Substances 0.000 description 1
229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
238000011533 pre-incubation Methods 0.000 description 1
239000002243 precursor Substances 0.000 description 1
230000037452 priming Effects 0.000 description 1
UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
125000001453 quaternary ammonium group Chemical group 0.000 description 1
238000000163 radioactive labelling Methods 0.000 description 1
230000001105 regulatory effect Effects 0.000 description 1
230000004044 response Effects 0.000 description 1
230000002441 reversible effect Effects 0.000 description 1
229920006395 saturated elastomer Polymers 0.000 description 1
239000000377 silicon dioxide Substances 0.000 description 1
239000001632 sodium acetate Substances 0.000 description 1
235000017281 sodium acetate Nutrition 0.000 description 1
239000001509 sodium citrate Substances 0.000 description 1
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
IHQKEDIOMGYHEB-UHFFFAOYSA-M sodium dimethylarsinate Chemical compound [Na+].C[As](C)([O-])=O IHQKEDIOMGYHEB-UHFFFAOYSA-M 0.000 description 1
229940063673 spermidine Drugs 0.000 description 1
238000003756 stirring Methods 0.000 description 1
101150012509 sub gene Proteins 0.000 description 1
239000000758 substrate Substances 0.000 description 1
239000000725 suspension Substances 0.000 description 1
230000002194 synthesizing effect Effects 0.000 description 1
QSUJAUYJBJRLKV-UHFFFAOYSA-M tetraethylazanium;fluoride Chemical compound [F-].CC[N+](CC)(CC)CC QSUJAUYJBJRLKV-UHFFFAOYSA-M 0.000 description 1
YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
229960003495 thiamine Drugs 0.000 description 1
DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
229940113082 thymine Drugs 0.000 description 1
231100000331 toxic Toxicity 0.000 description 1
230000002588 toxic effect Effects 0.000 description 1
238000001890 transfection Methods 0.000 description 1
238000012546 transfer Methods 0.000 description 1
230000009466 transformation Effects 0.000 description 1
238000011269 treatment regimen Methods 0.000 description 1
125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
239000012588 trypsin Substances 0.000 description 1
125000000430 tryptophan group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C2=C([H])C([H])=C([H])C([H])=C12 0.000 description 1
210000004881 tumor cell Anatomy 0.000 description 1
125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
230000029812 viral genome replication Effects 0.000 description 1
239000011691 vitamin B1 Substances 0.000 description 1
235000010374 vitamin B1 Nutrition 0.000 description 1
NLIVDORGVGAOOJ-MAHBNPEESA-M xylene cyanol Chemical compound [Na+].C1=C(C)C(NCC)=CC=C1C(\C=1C(=CC(OS([O-])=O)=CC=1)OS([O-])=O)=C\1C=C(C)\C(=[NH+]/CC)\C=C/1 NLIVDORGVGAOOJ-MAHBNPEESA-M 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/565—IFN-beta
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Microbiology (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Toxicology (AREA)
Gastroenterology & Hepatology (AREA)
Medicinal Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Saccharide Compounds (AREA)
Peptides Or Proteins (AREA)

NL8100558A 1980-02-06 1981-02-05 Recombinant dna-techniek voor de bereiding van een op menselijk interferon lijkend eiwit; gen voor de expressie van een dergelijk eiwit; subeenheid van een dergelijk gen; werkwijze voor de bereiding van een dergelijk gen of een dergelijke subeenheid; plasmiderecombinant; werkwijze voor de bereiding daarvan; cel met daarin een dergelijk gen, subeenheid daarvan of plasmiderecombinant; werkwijze voor de bereiding van een dergelijke cel; werkwijze voor de bereiding van een eiwit; aldus bereid eiwit. NL8100558A (nl)

Applications Claiming Priority (12)

Application Number	Priority Date	Filing Date	Title
GB8003947		1980-02-06
GB8003947		1980-02-06
GB8006712		1980-02-28
GB8006712		1980-02-28
GB8012666		1980-04-17
GB8012666		1980-04-17
GB8013592		1980-04-24
GB8013592		1980-04-24
GB8015646		1980-05-12
GB8015646		1980-05-12
GB8036951		1980-11-18
GB8036951		1980-11-18

Publications (1)

Publication Number	Publication Date
NL8100558A true NL8100558A (nl)	1981-09-01

Family

ID=27546780

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NL8100558A NL8100558A (nl)	1980-02-06	1981-02-05	Recombinant dna-techniek voor de bereiding van een op menselijk interferon lijkend eiwit; gen voor de expressie van een dergelijk eiwit; subeenheid van een dergelijk gen; werkwijze voor de bereiding van een dergelijk gen of een dergelijke subeenheid; plasmiderecombinant; werkwijze voor de bereiding daarvan; cel met daarin een dergelijk gen, subeenheid daarvan of plasmiderecombinant; werkwijze voor de bereiding van een dergelijke cel; werkwijze voor de bereiding van een eiwit; aldus bereid eiwit.

Country Status (8)

Country	Link
AU (1)	AU545758B2 (da)
DE (1)	DE3103714A1 (da)
DK (1)	DK33181A (da)
FR (1)	FR2481316B1 (da)
IL (1)	IL61994A0 (da)
IT (1)	IT1170688B (da)
NL (1)	NL8100558A (da)
SE (1)	SE8100581L (da)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DK159976C (da) *	1979-06-01	1991-05-27	Searle & Co	Plasmidvektorer, fremgangsmaade til fremstilling deraf, bakterieceller transformeret med plasmidvektorer samt udtrykkelse af protein i transformerede bakterieceller
DE3005897A1 (de) *	1980-02-16	1981-09-03	Hoechst Ag, 6000 Frankfurt	Genprodukt eines hoeheren organismus aus einem dieses gen enhtaltenden mikroorganismus
DE3005843A1 (de) *	1980-02-16	1981-09-10	Hoechst Ag, 6000 Frankfurt	Genprodukt eines hoeheren organismus aus einem dieses gen enthaltenden mikroorganismus
ZA811368B (en) *	1980-03-24	1982-04-28	Genentech Inc	Bacterial polypedtide expression employing tryptophan promoter-operator
EP0041313B1 (en)	1980-04-03	1990-09-12	Biogen, Inc.	Dna sequences, recombinant dna molecules and processes for producing human fibroblast interferon
GR81946B (da) *	1980-09-25	1984-12-12	Genentech Inc
DE3932311A1 (de) *	1989-09-28	1991-04-11	Boehringer Ingelheim Int	Verfahren zur reinigung von beta-interferon

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
AU538665B2 (en) *	1979-10-30	1984-08-23	Juridical Foundation, Japanese Foundation For Cancer Research	Human interferon dna
IL58765A (en) *	1979-11-21	1986-09-30	Yeda Res & Dev	Process for the production of essentially pure messenger rna of human fibroblast interferon and process for the production of interferon beta
DE3005843A1 (de) *	1980-02-16	1981-09-10	Hoechst Ag, 6000 Frankfurt	Genprodukt eines hoeheren organismus aus einem dieses gen enthaltenden mikroorganismus
EP0041313B1 (en) *	1980-04-03	1990-09-12	Biogen, Inc.	Dna sequences, recombinant dna molecules and processes for producing human fibroblast interferon
DE3176011D1 (en) *	1980-06-12	1987-04-23	Japan Found Cancer	Plasmid
GR81946B (da) *	1980-09-25	1984-12-12	Genentech Inc

1981
- 1981-01-23 DK DK33181A patent/DK33181A/da not_active Application Discontinuation
- 1981-01-27 IL IL61994A patent/IL61994A0/xx unknown
- 1981-01-28 SE SE8100581A patent/SE8100581L/ not_active Application Discontinuation
- 1981-01-30 AU AU66787/81A patent/AU545758B2/en not_active Ceased
- 1981-02-04 IT IT47715/81A patent/IT1170688B/it active
- 1981-02-04 DE DE19813103714 patent/DE3103714A1/de not_active Withdrawn
- 1981-02-05 NL NL8100558A patent/NL8100558A/nl not_active Application Discontinuation
- 1981-02-05 FR FR8102223A patent/FR2481316B1/fr not_active Expired

Also Published As

Publication number	Publication date
DK33181A (da)	1981-08-07
FR2481316B1 (fr)	1985-07-12
IL61994A0 (en)	1981-02-27
FR2481316A1 (fr)	1981-10-30
DE3103714A1 (de)	1982-11-25
IT1170688B (it)	1987-06-03
IT8147715A0 (it)	1981-02-04
AU545758B2 (en)	1985-08-01
AU6678781A (en)	1981-08-13
SE8100581L (sv)	1981-08-07

Legal Events

Date	Code	Title
1982-02-01	A1B	A search report has been drawn up
1982-07-01	A1B	A search report has been drawn up
1983-03-01	BV	The patent application has lapsed
1984-08-01	BV	The patent application has lapsed
1985-01-01	A85	Still pending on 85-01-01
1988-04-05	BV	The patent application has lapsed

Publication	Publication Date	Title
DK170236B1 (da)	1995-07-10	Rekombinante DNA-molekyler, fremgangsmåde til fremstilling af et polypeptid af IFN-alfa typen og fremgangsmåde til udvælgelse af DNA-sekvenser, der koder for polypeptider af IFN-alfa typen
JP2642291B2 (ja)	1997-08-20	ハイブリド型ヒト白血球インタフェロンをコードするｄｎａ
EP0048970A2 (en)	1982-04-07	Polypeptides, process for their microbial production, intermediates therefor and compositions containing them
EP0041313A2 (en)	1981-12-09	DNA sequences, recombinant DNA molecules and processes for producing human fibroblast interferon
McGeoch	1979	Structure of the gene N: gene NS intercistronic junction in the genome of vesicular stomatitis virus
NL8103151A (nl)	1982-02-01	Polypeptiden, farmaceutische preparaten, die deze poly- peptiden bevatten en werkwijzen ter bereiding ervan.
CN113811611A (zh)	2021-12-17	无毒cas9酶及其应用
Sawahel	2002	The production of transgenic potato plants expressing human alpha-interferon using lipofectin-mediated transformation
Young et al.	1980	Bacteriophage T4 gene transcription studied by hybridization to cloned restriction fragments
NL8100558A (nl)	1981-09-01	Recombinant dna-techniek voor de bereiding van een op menselijk interferon lijkend eiwit; gen voor de expressie van een dergelijk eiwit; subeenheid van een dergelijk gen; werkwijze voor de bereiding van een dergelijk gen of een dergelijke subeenheid; plasmiderecombinant; werkwijze voor de bereiding daarvan; cel met daarin een dergelijk gen, subeenheid daarvan of plasmiderecombinant; werkwijze voor de bereiding van een dergelijke cel; werkwijze voor de bereiding van een eiwit; aldus bereid eiwit.
Larsson et al.	1986	VA RNAs from avian and human adenoviruses: dramatic differences in length, sequence, and gene location
US6201115B1 (en)	2001-03-13	Amplifying sequences, vectors comprising these sequences and their uses in compositions for the expression of nucleotide sequences in transfected cells therapeutic and vaccine applications
JPS61501747A (ja)	1986-08-21	核酸配列の検出方法
GB2068970A (en)	1981-08-19	Recombinant DNA Technique for the Preparation of a Protein Resembling Human Interferon
Watanabe et al.	1986	In vitro viral RNA synthesis by a subcellular fraction of TMV-inoculated tobacco protoplasts
JPS6156199A (ja)	1986-03-20	新規ヒトインタ−フエロンα類
CN111518812B (zh)	2023-06-27	一种编辑绵羊FGF5基因实现选择性剪接的sgRNA、成套核酸分子和应用
JPH02291267A (ja)	1990-12-03	α―フェトプロテイン遺伝子のエンハンサーに結合する蛋白質をコードするＤＮＡ
US4921802A (en)	1990-05-01	Plant virus cDNA
Koronakis et al.	1985	The traM gene of the resistance plasmid R1: comparison with the corresponding sequence of the Escherichia coli F factor
Wosnick et al.	1989	Total chemical synthesis and expression in Escherichia coli of a maize glutathione-transferase (GST) gene
Tomarev et al.	1982	Molecular cloning of double-stranded cDNA from the eye lens of the frog Rana temporaria: construction of the cDNA clonotheque and identification of a clone containing the nucleotide sequences of the γ-crystallin gene: Gene bank; clone library; recombinant DNA; ophthalmology; poly (A)+ RNA
CN114457077A (zh)	2022-05-10	靶向新型冠状病毒rna及其反义链utr的脱氧核酶及其应用
Gerald et al.	1984	Expression of a DNA replication gene cluster in bacteriophage T4: genetic linkage and the control of gene product interactions
IE51030B1 (en)	1986-09-17	Recombinant dna technique for the preparation of a protein resembling human interferon