NL8703070A - Werkwijze voor het transformeren van cellen. - Google Patents

Werkwijze voor het transformeren van cellen. Download PDF

Info

Publication number: NL8703070A
Authority: NL; Netherlands
Prior art keywords: hypertonic; cells; bacillus thuringiensis; medium; per
Prior art date: 1986-12-22

Application number

NL8703070A

Other languages

English (en)

Dutch (nl)

Original Assignee

Sandoz Ag

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1986-12-22

Filing date

1987-12-18

Publication date

1988-07-18

1987-12-18 Application filed by Sandoz Ag filed Critical Sandoz Ag

1988-07-18 Publication of NL8703070A publication Critical patent/NL8703070A/nl

Links

238000000034 method Methods 0.000 title claims description 32
230000001131 transforming effect Effects 0.000 title claims description 5
241000193388 Bacillus thuringiensis Species 0.000 claims description 46
229940097012 bacillus thuringiensis Drugs 0.000 claims description 45
239000002609 medium Substances 0.000 claims description 22
108020004414 DNA Proteins 0.000 claims description 18
239000002202 Polyethylene glycol Substances 0.000 claims description 15
229920001223 polyethylene glycol Polymers 0.000 claims description 15
102000016943 Muramidase Human genes 0.000 claims description 13
108010014251 Muramidase Proteins 0.000 claims description 13
108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims description 13
238000004113 cell culture Methods 0.000 claims description 13
229960000274 lysozyme Drugs 0.000 claims description 13
235000010335 lysozyme Nutrition 0.000 claims description 13
239000004325 lysozyme Substances 0.000 claims description 13
239000012736 aqueous medium Substances 0.000 claims description 12
150000001720 carbohydrates Chemical class 0.000 claims description 9
108091029865 Exogenous DNA Proteins 0.000 claims description 5
230000000749 insecticidal effect Effects 0.000 claims description 4
239000000203 mixture Substances 0.000 claims description 3
241000238631 Hexapoda Species 0.000 claims description 2
159000000003 magnesium salts Chemical class 0.000 claims description 2
238000002360 preparation method Methods 0.000 claims description 2
210000004027 cell Anatomy 0.000 description 33
230000009466 transformation Effects 0.000 description 14
239000013612 plasmid Substances 0.000 description 10
239000000725 suspension Substances 0.000 description 8
239000006285 cell suspension Substances 0.000 description 7
210000001938 protoplast Anatomy 0.000 description 7
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
241000894006 Bacteria Species 0.000 description 5
150000003839 salts Chemical class 0.000 description 5
239000000243 solution Substances 0.000 description 5
IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 4
108090000623 proteins and genes Proteins 0.000 description 4
230000035484 reaction time Effects 0.000 description 4
239000007858 starting material Substances 0.000 description 4
235000014469 Bacillus subtilis Nutrition 0.000 description 3
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
229930006000 Sucrose Natural products 0.000 description 3
239000004098 Tetracycline Substances 0.000 description 3
239000006143 cell culture medium Substances 0.000 description 3
238000010367 cloning Methods 0.000 description 3
230000021615 conjugation Effects 0.000 description 3
230000000694 effects Effects 0.000 description 3
230000012010 growth Effects 0.000 description 3
238000011534 incubation Methods 0.000 description 3
230000003204 osmotic effect Effects 0.000 description 3
239000008188 pellet Substances 0.000 description 3
239000000575 pesticide Substances 0.000 description 3
239000011780 sodium chloride Substances 0.000 description 3
239000005720 sucrose Substances 0.000 description 3
229960002180 tetracycline Drugs 0.000 description 3
229930101283 tetracycline Natural products 0.000 description 3
235000019364 tetracycline Nutrition 0.000 description 3
150000003522 tetracyclines Chemical class 0.000 description 3
231100000331 toxic Toxicity 0.000 description 3
230000002588 toxic effect Effects 0.000 description 3
238000011426 transformation method Methods 0.000 description 3
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 2
241000283690 Bos taurus Species 0.000 description 2
241000254173 Coleoptera Species 0.000 description 2
241000255925 Diptera Species 0.000 description 2
QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
241000255777 Lepidoptera Species 0.000 description 2
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
241001465754 Metazoa Species 0.000 description 2
239000001888 Peptone Substances 0.000 description 2
108010080698 Peptones Proteins 0.000 description 2
XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
150000001413 amino acids Chemical class 0.000 description 2
239000003242 anti bacterial agent Substances 0.000 description 2
229940088710 antibiotic agent Drugs 0.000 description 2
230000008901 benefit Effects 0.000 description 2
IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 2
229940041514 candida albicans extract Drugs 0.000 description 2
239000012895 dilution Substances 0.000 description 2
238000010790 dilution Methods 0.000 description 2
239000000284 extract Substances 0.000 description 2
238000005187 foaming Methods 0.000 description 2
230000002068 genetic effect Effects 0.000 description 2
239000000819 hypertonic solution Substances 0.000 description 2
229940021223 hypertonic solution Drugs 0.000 description 2
239000004615 ingredient Substances 0.000 description 2
244000005700 microbiome Species 0.000 description 2
238000002156 mixing Methods 0.000 description 2
230000007935 neutral effect Effects 0.000 description 2
235000019319 peptone Nutrition 0.000 description 2
230000008929 regeneration Effects 0.000 description 2
238000011069 regeneration method Methods 0.000 description 2
229910052710 silicon Inorganic materials 0.000 description 2
239000010703 silicon Substances 0.000 description 2
229940104230 thymidine Drugs 0.000 description 2
239000012138 yeast extract Substances 0.000 description 2
BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
229920001817 Agar Polymers 0.000 description 1
GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
241000193830 Bacillus <bacterium> Species 0.000 description 1
244000063299 Bacillus subtilis Species 0.000 description 1
240000001817 Cereus hexagonus Species 0.000 description 1
101710151559 Crystal protein Proteins 0.000 description 1
102000016736 Cyclin Human genes 0.000 description 1
108050006400 Cyclin Proteins 0.000 description 1
241001631715 Dendrolimus Species 0.000 description 1
241000196324 Embryophyta Species 0.000 description 1
241000588724 Escherichia coli Species 0.000 description 1
241000192125 Firmicutes Species 0.000 description 1
GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
241001625930 Luria Species 0.000 description 1
-1 MnSO 4 Chemical class 0.000 description 1
244000061176 Nicotiana tabacum Species 0.000 description 1
235000002637 Nicotiana tabacum Nutrition 0.000 description 1
239000008118 PEG 6000 Substances 0.000 description 1
229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 1
OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 1
241000589540 Pseudomonas fluorescens Species 0.000 description 1
108020004511 Recombinant DNA Proteins 0.000 description 1
101100463786 Zea mays PG14 gene Proteins 0.000 description 1
239000002253 acid Substances 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
238000005273 aeration Methods 0.000 description 1
239000008272 agar Substances 0.000 description 1
230000003698 anagen phase Effects 0.000 description 1
230000010310 bacterial transformation Effects 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
239000000872 buffer Substances 0.000 description 1
230000003197 catalytic effect Effects 0.000 description 1
230000007910 cell fusion Effects 0.000 description 1
210000000170 cell membrane Anatomy 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
238000011161 development Methods 0.000 description 1
230000018109 developmental process Effects 0.000 description 1
AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 1
150000002016 disaccharides Chemical class 0.000 description 1
239000002158 endotoxin Substances 0.000 description 1
235000013305 food Nutrition 0.000 description 1
238000009472 formulation Methods 0.000 description 1
230000030414 genetic transfer Effects 0.000 description 1
230000006872 improvement Effects 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
238000001727 in vivo Methods 0.000 description 1
230000001939 inductive effect Effects 0.000 description 1
230000003993 interaction Effects 0.000 description 1
230000000968 intestinal effect Effects 0.000 description 1
238000002955 isolation Methods 0.000 description 1
239000002655 kraft paper Substances 0.000 description 1
239000008101 lactose Substances 0.000 description 1
229910001629 magnesium chloride Inorganic materials 0.000 description 1
VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
239000011976 maleic acid Substances 0.000 description 1
150000002696 manganese Chemical class 0.000 description 1
SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
229910000357 manganese(II) sulfate Inorganic materials 0.000 description 1
230000007246 mechanism Effects 0.000 description 1
150000002772 monosaccharides Chemical class 0.000 description 1
239000002777 nucleoside Substances 0.000 description 1
125000003835 nucleoside group Chemical group 0.000 description 1
235000015097 nutrients Nutrition 0.000 description 1
238000005457 optimization Methods 0.000 description 1
230000001717 pathogenic effect Effects 0.000 description 1
230000007170 pathology Effects 0.000 description 1
229940066779 peptones Drugs 0.000 description 1
229920001184 polypeptide Polymers 0.000 description 1
239000000843 powder Substances 0.000 description 1
102000004196 processed proteins & peptides Human genes 0.000 description 1
108090000765 processed proteins & peptides Proteins 0.000 description 1
239000002904 solvent Substances 0.000 description 1
238000001228 spectrum Methods 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
238000003786 synthesis reaction Methods 0.000 description 1
238000012360 testing method Methods 0.000 description 1
VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
108010046845 tryptones Proteins 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host

Landscapes

Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biomedical Technology (AREA)
Organic Chemistry (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Wood Science & Technology (AREA)
Microbiology (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Cell Biology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

NL8703070A 1986-12-22 1987-12-18 Werkwijze voor het transformeren van cellen. NL8703070A (nl)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
GB868630527A GB8630527D0 (en)	1986-12-22	1986-12-22	Organic compounds
GB8630527		1986-12-22

Publications (1)

Publication Number	Publication Date
NL8703070A true NL8703070A (nl)	1988-07-18

Family

ID=10609388

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NL8703070A NL8703070A (nl)	1986-12-22	1987-12-18	Werkwijze voor het transformeren van cellen.

Country Status (14)

Country	Link
JP (1)	JPS63181995A (fr)
AU (1)	AU611404B2 (fr)
BE (1)	BE1000309A4 (fr)
CA (1)	CA1304705C (fr)
CH (1)	CH674991A5 (fr)
DE (1)	DE3742429A1 (fr)
FR (1)	FR2608624B1 (fr)
GB (2)	GB8630527D0 (fr)
IE (1)	IE59334B1 (fr)
IL (1)	IL84890A (fr)
IT (1)	IT1230117B (fr)
NL (1)	NL8703070A (fr)
NZ (1)	NZ223013A (fr)
ZA (1)	ZA879616B (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
FI892359A7 (fi) *	1988-05-20	1989-11-21	Ciba Geigy Ag	Transformering av bacillus thuringiensis.
CN106544298B (zh) *	2016-10-27	2020-03-24	广东省微生物研究所	一种芽胞杆菌感受态细胞的制备方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS57186492A (en) *	1981-04-17	1982-11-16	Kyowa Hakko Kogyo Co Ltd	Transformation of bacterium
JPS60188069A (ja) *	1984-03-08	1985-09-25	Nakano Vinegar Co Ltd	酢酸菌の環状ｄνａ導入法
GB8425487D0 (en) *	1984-10-09	1984-11-14	Agricultural Genetics Co	Strain of bacillus thuringiensis
GB8523768D0 (en) *	1985-09-26	1985-10-30	Antibioticos Sa	Streptomyces wadayamensis

1986
- 1986-12-22 GB GB868630527A patent/GB8630527D0/en active Pending
1987
- 1987-12-15 DE DE19873742429 patent/DE3742429A1/de not_active Withdrawn
- 1987-12-18 IT IT8748728A patent/IT1230117B/it active
- 1987-12-18 CH CH4951/87A patent/CH674991A5/de not_active IP Right Cessation
- 1987-12-18 FR FR878717778A patent/FR2608624B1/fr not_active Expired - Lifetime
- 1987-12-18 NL NL8703070A patent/NL8703070A/nl not_active Application Discontinuation
- 1987-12-21 JP JP62325160A patent/JPS63181995A/ja active Pending
- 1987-12-21 AU AU82866/87A patent/AU611404B2/en not_active Ceased
- 1987-12-21 BE BE8701466A patent/BE1000309A4/fr not_active IP Right Cessation
- 1987-12-21 IE IE346887A patent/IE59334B1/en not_active IP Right Cessation
- 1987-12-21 NZ NZ223013A patent/NZ223013A/xx unknown
- 1987-12-21 GB GB8729726A patent/GB2199044B/en not_active Expired - Lifetime
- 1987-12-21 CA CA000554916A patent/CA1304705C/fr not_active Expired - Lifetime
- 1987-12-21 IL IL84890A patent/IL84890A/xx not_active IP Right Cessation
- 1987-12-22 ZA ZA879616A patent/ZA879616B/xx unknown

Also Published As

Publication number	Publication date
ZA879616B (en)	1989-08-30
NZ223013A (en)	1991-01-29
GB8630527D0 (en)	1987-02-04
AU8286687A (en)	1988-06-23
BE1000309A4 (fr)	1988-10-18
GB8729726D0 (en)	1988-02-03
GB2199044A (en)	1988-06-29
CA1304705C (fr)	1992-07-07
CH674991A5 (fr)	1990-08-15
IT8748728A0 (it)	1987-12-18
DE3742429A1 (de)	1988-06-30
FR2608624B1 (fr)	1990-03-09
AU611404B2 (en)	1991-06-13
IE873468L (en)	1988-06-22
IE59334B1 (en)	1994-02-09
FR2608624A1 (fr)	1988-06-24
JPS63181995A (ja)	1988-07-27
IT1230117B (it)	1991-10-07
IL84890A (en)	1992-12-01
IL84890A0 (en)	1988-06-30
GB2199044B (en)	1991-03-27

Legal Events

Date	Code	Title	Description
1995-02-01	BV	The patent application has lapsed

Publication	Publication Date	Title
AU596125B2 (en)	1990-04-26	Improvements in or relating to organic compounds
DE69434807T2 (de)	2007-03-29	Methode und system zur erhöhten produktion von kommerziell wichtigen exoproteinen in gram-positiven bakterien
Vilas-Bôas et al.	1998	Bacillus thuringiensis conjugation under environmental conditions
Thamthiankul et al.	2004	Improving the insecticidal activity of Bacillus thuringiensis subsp. aizawai against Spodoptera exigua by chromosomal expression of a chitinase gene
AU638208B2 (en)	1993-06-24	Bacillus thuringiensis transformation
Park et al.	2001	Construction and characterization of a recombinant Bacillus thuringiensis subsp. israelensis strain that produces Cry11B
JPH07501323A (ja)	1995-02-09	新規な鞘翅類活性バシラスチューリンゲンシス単離体及び鞘翅類活性毒素をコードする遺伝子
NL8703070A (nl)	1988-07-18	Werkwijze voor het transformeren van cellen.
FR2740472A1 (fr)	1997-04-30	Nouvelles souches de bacillus thuringiensis et composition pesticide les contenant
US8097446B2 (en)	2012-01-17	Staphylococus aureus strain CYL1892
Ozawa et al.	1986	Involvement of a transmissible plasmid in heat-stable exotoxin and delta-endotoxin production in Bacillus thuringiensis subspecies Darmstadiensis
Yari et al.	2002	Effects of Protoplast Fusion on δ-endotoxin Production in Bacillus thuringiensis Spp.(H14)
RU2167528C1 (ru)	2001-05-27	Штамм bacillus thuringiensis subsp.kurstaki ipm-46, активный против насекомых из отрядов coleoptera и lepidoptera
RU2103363C1 (ru)	1998-01-27	Рекомбинантная плазмидная днк рвтn ii, определяющая синтез кристаллического инсектицидного дельта-эндотоксина cry iiia-типа, способ ее конструирования и штамм бактерий pseudomonas putida, содержащий рекомбинантную плазмиду днк рвтn ii-продуцент кристаллического инсектицидного дельта-эндотоксина cry iiia-типа, активного против насекомых отряда coleoptera
Clark et al.	1984	The Bacillus thuringiensis genetic systems
JPH02219567A (ja)	1990-09-03	バチルス・チュリンジェンシス変異イスラエレンシスから遺伝子工学的にえられた殺幼虫性毒素を産生するバチルス・スフェリカス
JP2000506739A (ja)	2000-06-06	殺虫性タンパク質の改良生産
JP2627425B2 (ja)	1997-07-09	バシラス・ツリンギーンシス型の微生物およびその創製方法
Clark	1987	Characterization of plasmids from Bacillus thuringiensis var. israelensis
EP0582541A2 (fr)	1994-02-09	Procédé pour la création de souches de Bacillus thuringiensis capables de conjugaison
Roh et al.	2004	Expression and characterization of a recombinant Cry1Ac crystal protein with enhanced green fluorescent protein in acrystalliferous Bacillus thuringiensis
Dobrzhanskaya et al.	2000	Characterization of a new Bacillus thuringiensis strain that is toxic to coleoptera
KR100432140B1 (ko)	2004-05-17	토양으로부터 분리된 바실러스 투린지엔시스 케이-1 균주 및 이 균주를 이용한 미생물 살충제
Iizuka et al.	1983	Comparative profiles of plasmid DNA and morphology of parasporal crystals in four strains of Bacillus thuringiensis subsp. darmstadiensis
Selinger	1995	Expression of Bacillus thuringiensis delta-endotoxin genes by rhizosphere inhabiting bacilli.