NO180593C - Fremgangsmåte for fremstilling, i Saccharomyces cerevisiae, av et heterologt protein i homogen form - Google Patents

Fremgangsmåte for fremstilling, i Saccharomyces cerevisiae, av et heterologt protein i homogen form

Info

Publication number
NO180593C
NO180593C NO891837A NO891837A NO180593C NO 180593 C NO180593 C NO 180593C NO 891837 A NO891837 A NO 891837A NO 891837 A NO891837 A NO 891837A NO 180593 C NO180593 C NO 180593C
Authority
NO
Norway
Prior art keywords
preparation
saccharomyces cerevisiae
heterologous protein
homologous
homologous heterologous
Prior art date
Application number
NO891837A
Other languages
English (en)
Norwegian (no)
Other versions
NO891837D0 (no
NO180593B (no
NO891837L (no
Inventor
Hansjoerg Treichler
Kenji Takabayashi
Dieter Heinrich Wolf
Jutta Heim
Original Assignee
Ciba Geigy Ag
Ucp Gen Pharma Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB888810524A external-priority patent/GB8810524D0/en
Priority claimed from GB888812627A external-priority patent/GB8812627D0/en
Priority claimed from GB898907110A external-priority patent/GB8907110D0/en
Application filed by Ciba Geigy Ag, Ucp Gen Pharma Ag filed Critical Ciba Geigy Ag
Publication of NO891837D0 publication Critical patent/NO891837D0/no
Publication of NO891837L publication Critical patent/NO891837L/no
Publication of NO180593B publication Critical patent/NO180593B/no
Publication of NO180593C publication Critical patent/NO180593C/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Liquid Crystal Substances (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
NO891837A 1988-05-04 1989-05-03 Fremgangsmåte for fremstilling, i Saccharomyces cerevisiae, av et heterologt protein i homogen form NO180593C (no)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB888810524A GB8810524D0 (en) 1988-05-04 1988-05-04 Improvements in production of polypeptides
GB888812627A GB8812627D0 (en) 1988-05-27 1988-05-27 Improvements in production of polypeptides
GB898907110A GB8907110D0 (en) 1988-05-04 1989-03-29 Improvements in the production of polypeptides

Publications (4)

Publication Number Publication Date
NO891837D0 NO891837D0 (no) 1989-05-03
NO891837L NO891837L (no) 1989-11-06
NO180593B NO180593B (no) 1997-02-03
NO180593C true NO180593C (no) 1997-05-14

Family

ID=27263890

Family Applications (1)

Application Number Title Priority Date Filing Date
NO891837A NO180593C (no) 1988-05-04 1989-05-03 Fremgangsmåte for fremstilling, i Saccharomyces cerevisiae, av et heterologt protein i homogen form

Country Status (16)

Country Link
EP (1) EP0341215B1 (de)
JP (1) JPH0824565B2 (de)
AT (1) ATE124086T1 (de)
AU (1) AU614121B2 (de)
CA (1) CA1340016C (de)
DE (1) DE68923123T2 (de)
DK (1) DK175701B1 (de)
ES (1) ES2072918T3 (de)
FI (1) FI103129B1 (de)
HU (1) HU218731B (de)
IE (1) IE66044B1 (de)
IL (1) IL90170A (de)
MX (1) MX169508B (de)
NO (1) NO180593C (de)
NZ (1) NZ228955A (de)
PT (1) PT90436B (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8809860D0 (en) * 1988-04-26 1988-06-02 Ciba Geigy Ag Process for production of polypeptides
US5879926A (en) * 1989-03-31 1999-03-09 Transgene S.A. Yeast strains for the production of mature heterologous proteins, especially hirudin
FR2645175B1 (fr) * 1989-03-31 1994-02-18 Transgene Sa Souche de saccharomyces cerevisiaeproductrice d'une proteine heterologue et procede de preparation de ladite proteine heterologue par fermentation de ladite souche
FR2645174B1 (fr) * 1989-03-31 1994-01-07 Transgene Sa Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant
US5663141A (en) * 1989-12-01 1997-09-02 Basf Aktiengesellschaft Hirudin/polyalkylene glycol conjugates and hirudin muteins
EP0539490B1 (de) * 1990-07-13 1995-11-08 Transkaryotic Therapies, Inc. Verfahren zum absuchen einer bibliothek
US5580734A (en) * 1990-07-13 1996-12-03 Transkaryotic Therapies, Inc. Method of producing a physical map contigous DNA sequences
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
CA2090969C (en) * 1990-09-04 2001-08-21 Russell Arthur Brierley Production of insulin-like growth factor-1 in methylotrophic yeast cells
DE69130872T2 (de) 1990-11-08 1999-08-26 Japan Energy Corp. Sekretionsvektor, diesen enthaltene transformierte Mikroorganismen und Herstellung von Produkten durch obigen Mikroorganismus
SG84484A1 (en) * 1991-02-25 2001-11-20 Ciba Geigy Ag Improved yeast vectors
AU661844B2 (en) * 1991-04-01 1995-08-10 Merck Sharp & Dohme Corp. Genes which influence (pichia) proteolytic activity, and uses therefor
HU214698B (hu) * 1992-04-09 1998-06-29 Gyógyszerkutató Intézet Kft. Eljárás rekombináns deszulfátó-hirudin HV-1 peptidek előállítására
DE69329540T2 (de) * 1992-09-04 2001-06-07 Novartis Ag, Basel Verfahren zur Herstellung von Proteaseinhibitoren
TW282490B (de) * 1992-12-15 1996-08-01 Ciba Geigy Ag
CN1113389A (zh) * 1993-08-04 1995-12-13 西巴-盖尔基股份公司 高分子量的脱硫水蛭素
DE4404168A1 (de) * 1994-02-10 1995-08-17 Hoechst Ag Hirudinderivate und Verfahren zu deren Herstellung
ATE207538T1 (de) 1995-02-09 2001-11-15 Behringwerke Ag Verfahren zur produktion von proteinen
ATE353953T1 (de) * 1995-03-20 2007-03-15 Novozymes As Wirtszelle, die reduzierte mengen einer metalloprotease exprimiert und methoden zur verwendung dieser wirtszelle zur proteinproduktion
DE19529997C1 (de) * 1995-08-16 1997-04-10 Hoechst Ag Verfahren zur Inaktivierung von Carboxypeptidase Y in hirudinhaltigen Kulturbrühen
WO1997012045A1 (en) * 1995-09-27 1997-04-03 Chiron Corporation Method of making a protease deficient host
DE69625623T2 (de) 1996-01-31 2003-11-06 Sumitomo Bakelite Co. Ltd., Tokio/Tokyo Verfahren zur Herstellung von in Epoxyharz eingekapselter Halbleitervorrichtung
FR2768743B1 (fr) 1997-09-23 2001-09-14 Bio Merieux Procede de lyse de micro-organisme
GB2338236B (en) * 1998-06-13 2003-04-09 Aea Technology Plc Microbiological cell processing
US7795205B2 (en) 2004-04-12 2010-09-14 Canyon Pharmaceuticals, Inc. Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor
EP2504355B1 (de) 2009-11-25 2015-07-22 Novo Nordisk A/S Verfahren zur herstellung von polypeptiden
JP7234932B2 (ja) 2017-10-10 2023-03-08 味の素株式会社 タンパク質の製造法
CN115836127B (zh) 2020-06-11 2026-03-13 味之素株式会社 蛋白的制造方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3342139A1 (de) * 1983-11-22 1985-05-30 Ciba-Geigy Ag, Basel Desulfatohirudine, verfahren zu ihrer herstellung und pharmazeutische mittel
JPS62224284A (ja) * 1986-03-25 1987-10-02 Agency Of Ind Science & Technol 微生物
GB8809860D0 (en) * 1988-04-26 1988-06-02 Ciba Geigy Ag Process for production of polypeptides
GB8817161D0 (en) * 1988-07-19 1988-08-24 Ciba Geigy Ag Modified proteins
GB8817160D0 (en) * 1988-07-19 1988-08-24 Ciba Geigy Ag Novel proteins

Also Published As

Publication number Publication date
NO891837D0 (no) 1989-05-03
MX169508B (es) 1993-07-08
IL90170A0 (en) 1989-12-15
JPH0824565B2 (ja) 1996-03-13
DE68923123D1 (de) 1995-07-27
IE891451L (en) 1989-11-04
JPH02104279A (ja) 1990-04-17
AU3382889A (en) 1989-11-09
NZ228955A (en) 1991-06-25
IL90170A (en) 1995-12-31
DE68923123T2 (de) 1995-11-30
EP0341215A3 (de) 1992-01-15
PT90436A (pt) 1989-11-30
DK217589D0 (da) 1989-05-03
PT90436B (pt) 1994-11-30
CA1340016C (en) 1998-08-25
ES2072918T3 (es) 1995-08-01
IE66044B1 (en) 1995-12-13
FI103129B (fi) 1999-04-30
ATE124086T1 (de) 1995-07-15
AU614121B2 (en) 1991-08-22
EP0341215B1 (de) 1995-06-21
DK217589A (da) 1989-11-05
FI892114L (fi) 1989-11-05
NO180593B (no) 1997-02-03
DK175701B1 (da) 2005-01-24
HUT54210A (en) 1991-01-28
NO891837L (no) 1989-11-06
FI892114A0 (fi) 1989-05-03
EP0341215A2 (de) 1989-11-08
FI103129B1 (fi) 1999-04-30
HU218731B (hu) 2000-11-28

Similar Documents

Publication Publication Date Title
NO891837L (no) Forbedringer i fremstilling av polypeptider.
DE68929115D1 (de) Verfahren zur Herstellung von menschlichem Serumalbumin in Hefe
ATE248925T1 (de) Verfahren zur herstellung von proteinen oder proteinhaltigen genprodukten
PT83761A (en) Method for producing mammalian interleukin-4
HU894572D0 (en) Process for producing peptones and dna sequences
AU3473489A (en) Expression of hepatisis b s and pres2proteins in methylotrophic yeasts
NO891716L (no) Ekspresjon av humant serumalbumin i metylotrofe gjaersorter
FR2645175B1 (fr) Souche de saccharomyces cerevisiaeproductrice d'une proteine heterologue et procede de preparation de ladite proteine heterologue par fermentation de ladite souche
DK0574347T3 (da) Hidtil ukendt svampeprotease
FR2645174B1 (fr) Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant
EP0684312A3 (de) Herstellung eines naturliches Süssungsmittel-Proteins.
WO1988000836A3 (fr) Immunosuppresseur et son procede de fabrication
SE9301583D0 (sv) Yeast strain and methods for expressing heterologous proteins in yeast
AU1321988A (en) The cloning of malaria-specific dna sequences: isolation of the gene for the 140 kd protein
FI882059A0 (fi) Menetelmä vierasproteiinien valmistamiseksi Streptomycessoluissa
EP0339569A3 (de) Expression vom HIV 24 KDA GAG Protein in methylotropischen Hefen
AU2278392A (en) Method for producing recombinant proteins and host cells used therein

Legal Events

Date Code Title Description
MK1K Patent expired