NO20000575L - Rekombinant ekspresjon av insulin C-peptid - Google Patents

Rekombinant ekspresjon av insulin C-peptid

Info

Publication number: NO20000575L
Authority: NO; Norway
Prior art keywords: insulin; peptide; recombinant expression; polypeptide; multimeric
Prior art date: 1997-08-07

Application number

NO20000575A

Other languages

English (en)

Norwegian (no)

Other versions

NO20000575D0 (no

Inventor

Stefan Stohl

Mathias Uhlen

Per-Ke Nygren

Per Jonasson

Original Assignee

Creative Peptides Sweden Ab

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1997-08-07

Filing date

2000-02-04

Publication date

2000-04-04

2000-02-04 Application filed by Creative Peptides Sweden Ab filed Critical Creative Peptides Sweden Ab

2000-02-04 Publication of NO20000575D0 publication Critical patent/NO20000575D0/no

2000-04-04 Publication of NO20000575L publication Critical patent/NO20000575L/no

Links

VOUAQYXWVJDEQY-QENPJCQMSA-N 33017-11-7 Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)NCC(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N1[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)CCC1 VOUAQYXWVJDEQY-QENPJCQMSA-N 0.000 title abstract 5
238000003259 recombinant expression Methods 0.000 title 1
229920001184 polypeptide Polymers 0.000 abstract 3
108090000765 processed proteins & peptides Proteins 0.000 abstract 3
102000004196 processed proteins & peptides Human genes 0.000 abstract 3
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 2
238000000034 method Methods 0.000 abstract 2
108010075254 C-Peptide Proteins 0.000 abstract 1
102000004877 Insulin Human genes 0.000 abstract 1
108090001061 Insulin Proteins 0.000 abstract 1
238000003776 cleavage reaction Methods 0.000 abstract 1
239000013604 expression vector Substances 0.000 abstract 1
229940125396 insulin Drugs 0.000 abstract 1
238000004519 manufacturing process Methods 0.000 abstract 1
102000039446 nucleic acids Human genes 0.000 abstract 1
108020004707 nucleic acids Proteins 0.000 abstract 1
150000007523 nucleic acids Chemical class 0.000 abstract 1
230000007017 scission Effects 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
General Health & Medical Sciences (AREA)
Zoology (AREA)
Diabetes (AREA)
Biophysics (AREA)
Bioinformatics & Cheminformatics (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Wood Science & Technology (AREA)
Medicinal Chemistry (AREA)
Gastroenterology & Hepatology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Endocrinology (AREA)
Plant Pathology (AREA)
Microbiology (AREA)
Toxicology (AREA)
Physics & Mathematics (AREA)
Chemical Kinetics & Catalysis (AREA)
Obesity (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Pharmacology & Pharmacy (AREA)
Animal Behavior & Ethology (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
General Chemical & Material Sciences (AREA)
Hematology (AREA)
Emergency Medicine (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

NO20000575A 1997-08-07 2000-02-04 Rekombinant ekspresjon av insulin C-peptid NO20000575L (no)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
GBGB9716790.2A GB9716790D0 (en)	1997-08-07	1997-08-07	Recombinant DNA molecules comprising multimeric copies of a gene sequence and expression thereof
PCT/GB1998/002382 WO1999007735A2 (fr)	1997-08-07	1998-08-07	Expression recombinee du peptide c de l'insuline

Publications (2)

Publication Number	Publication Date
NO20000575D0 NO20000575D0 (no)	2000-02-04
NO20000575L true NO20000575L (no)	2000-04-04

Family

ID=10817184

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO20000575A NO20000575L (no)	1997-08-07	2000-02-04	Rekombinant ekspresjon av insulin C-peptid

Country Status (21)

Country	Link
US (1)	US6558924B1 (fr)
EP (1)	EP1001980A1 (fr)
JP (1)	JP2001512745A (fr)
KR (1)	KR20010022590A (fr)
CN (1)	CN1268141A (fr)
AU (1)	AU750685B2 (fr)
BG (1)	BG104161A (fr)
BR (1)	BR9811850A (fr)
CA (1)	CA2297382A1 (fr)
EA (1)	EA200000202A1 (fr)
EE (1)	EE200000072A (fr)
GB (1)	GB9716790D0 (fr)
HU (1)	HUP0004626A1 (fr)
IL (1)	IL134372A0 (fr)
IS (1)	IS5368A (fr)
NO (1)	NO20000575L (fr)
NZ (1)	NZ502450A (fr)
PL (1)	PL339085A1 (fr)
SK (1)	SK1532000A3 (fr)
TR (1)	TR200000288T2 (fr)
WO (1)	WO1999007735A2 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US20040005669A1 (en) *	1997-08-07	2004-01-08	Stefan Stahl	Recombinant expression of insulin C-peptide
FR2810675B1 (fr) *	2000-06-22	2002-09-27	Pf Medicament	Construction modifiee en aval du codon d'initiation pour la surexpression de proteines recombinantes
FR2810674A1 (fr) *	2000-06-22	2001-12-28	Pf Medicament	Construction modifiee en amont du codon d'initiation pour la surexpression de proteines recombinantes
AU2002233230B2 (en) *	2000-12-20	2007-02-01	F. Hoffmann-La Roche Ag	Erythropoietin conjugates
CN101044154A (zh) *	2002-02-14	2007-09-26	威廉·J·鲁特	治疗宿主中切割的嵌合分子
WO2004047871A2 (fr)	2002-11-26	2004-06-10	Nobex Corporation	Composes natriuretiques modifies, leurs conjugues et leur utilisation
US20050026826A1 (en) *	2003-01-17	2005-02-03	Margarethe Hoenig	Feline proinsulin, insulin and constituent peptides
CN1298742C (zh) *	2003-06-03	2007-02-07	上海新药研究开发中心	一种适合于高效表达的融合蛋白及其生产方法
WO2005087811A2 (fr) *	2004-03-10	2005-09-22	Creighton University	Recepteurs des oestrogenes et procedes d'utilisation associes
KR20130115086A (ko)	2010-05-17	2013-10-21	세빅스 인코포레이티드	페길화된 c-펩티드
US8962553B2 (en)	2011-11-17	2015-02-24	Cebix Ab	Method of treating a diabetic subject having a microvascular impairment disorder by a pegylated C-peptide
SG11201405721VA (en) *	2012-03-19	2014-10-30	Madeleine Pharmaceuticals Ρτy Ltd	Method of producing a recombinant peptide
WO2016145389A1 (fr)	2015-03-12	2016-09-15	Board Of Trustees Of Michigan State University	Compositions et procédés pour mesurer la liaison de peptide-c et pour diagnostiquer des maladies à médiation immunitaire
CN109705221B (zh) *	2018-12-27	2021-03-09	美康生物科技股份有限公司	C肽免疫原及其单克隆抗体对及该抗体对在c肽磁微粒化学发光免疫试剂中的应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS5210872B2 (fr)	1973-07-14	1977-03-26
GB2068971B (en)	1980-01-30	1983-06-08	Searle & Co	Recombinant dna techniques
CA1213537A (fr)	1984-05-01	1986-11-04	Canadian Patents And Development Limited - Societe Canadienne Des Brevets Et D'exploitation Limitee	Methode d'expression de polypeptides
US5013653A (en)	1987-03-20	1991-05-07	Creative Biomolecules, Inc.	Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
EP0305500B1 (fr)	1987-03-20	1994-11-09	Creative Biomolecules, Inc.	Procede de purification de polypeptides recombinants
SE501169C2 (sv)	1988-02-05	1994-11-28	Aake Nygren	Framställning av serumalbuminbindande fusionsproteiner vilka innehåller protein G-domäner
US5130236A (en)	1989-12-07	1992-07-14	Eli Lilly And Company	Process for producing des(64,65)-proinsulin
US5595887A (en)	1990-07-16	1997-01-21	Bionebraska, Inc.	Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment
GB9015825D0 (en)	1990-07-18	1990-09-05	Ciba Geigy Ag	In vitro processing of fusion proteins
JPH07108232B2 (ja)	1990-10-09	1995-11-22	エム・ディ・リサーチ株式会社	ペプチド又は蛋白質の製造方法
US5953418A (en)	1995-06-14	1999-09-14	David Hall	Providing selective data broadcast receiver addressability
WO1997023639A1 (fr)	1995-12-22	1997-07-03	Toray Industries, Inc.	Procede de production de proteines fusionnees biologiquement actives

1997
- 1997-08-07 GB GBGB9716790.2A patent/GB9716790D0/en active Pending
1998
- 1998-08-07 SK SK153-2000A patent/SK1532000A3/sk unknown
- 1998-08-07 HU HU0004626A patent/HUP0004626A1/hu unknown
- 1998-08-07 EP EP98937689A patent/EP1001980A1/fr not_active Withdrawn
- 1998-08-07 AU AU86407/98A patent/AU750685B2/en not_active Ceased
- 1998-08-07 US US09/485,286 patent/US6558924B1/en not_active Expired - Fee Related
- 1998-08-07 BR BR9811850-1A patent/BR9811850A/pt not_active Application Discontinuation
- 1998-08-07 PL PL98339085A patent/PL339085A1/xx not_active Application Discontinuation
- 1998-08-07 TR TR2000/00288T patent/TR200000288T2/xx unknown
- 1998-08-07 IL IL13437298A patent/IL134372A0/xx unknown
- 1998-08-07 WO PCT/GB1998/002382 patent/WO1999007735A2/fr not_active Ceased
- 1998-08-07 CN CN98808075A patent/CN1268141A/zh active Pending
- 1998-08-07 KR KR1020007001182A patent/KR20010022590A/ko not_active Withdrawn
- 1998-08-07 NZ NZ502450A patent/NZ502450A/xx unknown
- 1998-08-07 JP JP2000506237A patent/JP2001512745A/ja active Pending
- 1998-08-07 EA EA200000202A patent/EA200000202A1/ru unknown
- 1998-08-07 EE EEP200000072A patent/EE200000072A/xx unknown
- 1998-08-07 CA CA002297382A patent/CA2297382A1/fr not_active Abandoned
2000
- 2000-02-01 IS IS5368A patent/IS5368A/is unknown
- 2000-02-04 NO NO20000575A patent/NO20000575L/no not_active Application Discontinuation
- 2000-02-17 BG BG104161A patent/BG104161A/xx unknown

Also Published As

Publication number	Publication date
SK1532000A3 (en)	2000-09-12
WO1999007735A3 (fr)	1999-04-29
EE200000072A (et)	2000-10-16
CA2297382A1 (fr)	1999-02-18
NO20000575D0 (no)	2000-02-04
IS5368A (is)	2000-02-01
US6558924B1 (en)	2003-05-06
KR20010022590A (ko)	2001-03-26
HUP0004626A1 (hu)	2001-04-28
WO1999007735A2 (fr)	1999-02-18
GB9716790D0 (en)	1997-10-15
EP1001980A1 (fr)	2000-05-24
TR200000288T2 (tr)	2000-06-21
AU8640798A (en)	1999-03-01
JP2001512745A (ja)	2001-08-28
PL339085A1 (en)	2000-12-04
IL134372A0 (en)	2001-04-30
BR9811850A (pt)	2000-08-08
NZ502450A (en)	2002-10-25
CN1268141A (zh)	2000-09-27
AU750685B2 (en)	2002-07-25
BG104161A (en)	2000-12-29
EA200000202A1 (ru)	2001-04-23

Legal Events

Date	Code	Title	Description
2002-05-21	FC2A	Withdrawal, rejection or dismissal of laid open patent application

Publication	Publication Date	Title
NO20000575L (no)	2000-04-04	Rekombinant ekspresjon av insulin C-peptid
NO20020861L (no)	2002-04-19	Nye B7-4-molekyler og anvendelser derav
BRPI9811940A (pt)	2000-09-05	vetor de expressão para um polipeptídeo de ligação da interleucina-18 (il-18-bp), célula hospedeira procariótica transformada com o referido vetor, processo para produção de il-18-bp, proteína de fusão compreendendo o referido polipeptídeo, bem como il-18bp modificada.
BR9813608B1 (pt)	2011-11-01	célula hospedeira de bacillus transgênica, e, processo para produzir um polipeptìdeo tendo atividade aminopeptidase.
BR0009392A (pt)	2002-01-08	Polipeptìdeo, sequência de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, métodos para produzir uma sequência de ácido nucleico mutante e para produzir um polipeptìdeo, mutante de um polipeptìdeo com atividade de alafa-amilase, e, uso do polipeptìdeo ou variante
WO1994003616A3 (fr)	1994-03-31	Vecteur de clonage et/ou de sequençage
BR9713294A (pt)	2000-10-17	"anticorpos contra proteìna relacionada a hormÈnio de paratiróide humano"
WO1995011984A3 (fr)	1995-07-06	Vecteur recombinant d'adenovirus et procedes d'utilisation
KR960010864A (ko)	1996-04-20	동물 세포의 형질 감염을 위한 재조합 dna 바이러스 벡터
PT728214E (pt)	2004-11-30	Linhas celulares estaveis capazes de expressar o gene de replicacao do virus adeno-associado
DE68928467D1 (de)	1998-01-08	RNS-Katalysator zur Spaltung von spezifischen RNS-Sequenzen
EP0776331A4 (fr)	1998-11-25	Structure d'ordre superieur et liaison d'acides nucleiques peptidiques
EP1798239A3 (fr)	2012-08-15	Récepteurs de protéine G-couplés et utilisations correspondantes
NO2007008I2 (no)	2011-02-21	Analoger av humant erytropoietin,DNA-sekvenser somkoder for disse, eukaryot vertscelle transfektert med DNA-sekvensene, preparat som omfatter analoge ne, anvendelse av analogene til økning av hematokritt, og fremgangsmåte for fremstilling av analogene.
BR9915821A (pt)	2001-10-23	Métodos para transformação de plantas para expressar delta-endotoxinas de bacillus thuringiensis
IE831211L (en)	1983-11-25	Cloning vectors for expression of exogenous protein
DE69933550D1 (de)	2006-11-23	Modifizierte adenoviren welche ein faserersatzprotein enthalten
BR9811865A (pt)	2000-08-15	Isolamento de um novo gene de fator de senescência, p23
WO2003022996A3 (fr)	2003-12-31	Synthese de conjugues polypeptides-oligomeres de l'insuline, et conjugues polypeptides-oligomeres de proinsuline, et modalites de leur synthese
ID27206A (id)	2001-03-08	Generasi produk-produk perbanyakan gena dari protein-protein fusi simerik melalui pemutusan endoprotease ubikuitus
AR012499A1 (es)	2000-10-18	Una secuencia quimerica de acido nucleico que codifica una proteina de fusion, un vector de expresion que comprende dicha secuencia quimerica, una celula huesped transformada que contiene dicho vector, la proteina de fusion obtenida y un metodo in vitro para la preparacion de un polipeptido recombin
NO972063L (no)	1997-05-05	Isolert BMP-reseptorkinaseprotein, DNA-sekvens, rekombinant ekspresjonsvektor samt vertscelle
BR9815496A (pt)	2001-12-18	Vacinas de ácido nucléico que codificam proteìna gde vìrus sincicial respiratório
WO2003099854A3 (fr)	2007-02-15	Procede de production enzymatique de peptides glp-2 (1-34) et glp-2 (1-33)
NO924192D0 (no)	1992-10-30	Biosyntetisk fremgangsmaate for fremstilling av kjemiske forbindelser