NO20071486L - Dyrking av humane embryonale stamceller - Google Patents

Dyrking av humane embryonale stamceller

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Publication number
NO20071486L
NO20071486L NO20071486A NO20071486A NO20071486L NO 20071486 L NO20071486 L NO 20071486L NO 20071486 A NO20071486 A NO 20071486A NO 20071486 A NO20071486 A NO 20071486A NO 20071486 L NO20071486 L NO 20071486L
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Norway
Prior art keywords
cells
stem cells
medium
human embryonic
embryonic stem
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Application number
NO20071486A
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English (en)
Other versions
NO344696B1 (no
Inventor
James A Thomson
Tenneille Ludwing
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Wisconsin Alumni Res Found
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Publication date
Application filed by Wisconsin Alumni Res Found filed Critical Wisconsin Alumni Res Found
Publication of NO20071486L publication Critical patent/NO20071486L/no
Publication of NO344696B1 publication Critical patent/NO344696B1/no

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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  • Health & Medical Sciences (AREA)
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  • Zoology (AREA)
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  • Biotechnology (AREA)
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  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Microbiology (AREA)
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  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Tidligere fremgangsmåter for dyrkning av humane embryonale stamceller har krevd enten fibroblast næringsceller eller et medium som er blitt eksponert for fibroblast næringsceller for å opprettholde stamcellene i en udifferensiert tilstand. Det er nå fiurmet at dersom høye nivåer av fibroblastvekstfaktor anvendes i et medium med gammaaminosmørsyre, pipecholinsyre, litium og lipider, vil stamcellene opprettholdes uendelig i udifferensiert tilstand gjermom mange passasjer, selv uten næringsceller eller kondisjonert medium. En humanisert matriks av humane proteiner kan anvendes som en grunnmatriks for å dyrke cellene. Nye linjer av humane embryonale stamceller er laget ved hjelp av disse kulturbetingelsene, der mediet og matriksen aldri er blitt eksponert for dyreceller, dyreproteiner, næringsceller eller kondisjonert medium. Effekt RV veldtlUdDr på ccUeprolffcreiiiig og dtfrcramlering
NO20071486A 2004-09-08 2007-03-21 Cellekulturmedium egnet for å opprettholde stamceller i en udifferensiert tilstand gjennom mange dyrkningspassasjer. NO344696B1 (no)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US60804004P 2004-09-08 2004-09-08
US69510005P 2005-06-29 2005-06-29
PCT/US2005/031839 WO2006029198A2 (en) 2004-09-08 2005-09-08 Culturing human embryonic stem cells

Publications (2)

Publication Number Publication Date
NO20071486L true NO20071486L (no) 2007-03-21
NO344696B1 NO344696B1 (no) 2020-03-09

Family

ID=35809711

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20071486A NO344696B1 (no) 2004-09-08 2007-03-21 Cellekulturmedium egnet for å opprettholde stamceller i en udifferensiert tilstand gjennom mange dyrkningspassasjer.

Country Status (16)

Country Link
US (2) US7442548B2 (no)
EP (2) EP1797172B1 (no)
JP (1) JP2008518585A (no)
KR (1) KR20070058584A (no)
AU (1) AU2005282414C1 (no)
BR (1) BRPI0514641A (no)
CA (1) CA2579652A1 (no)
DK (1) DK3196296T3 (no)
ES (2) ES2708384T3 (no)
GB (1) GB2432835B (no)
IL (1) IL181438A (no)
IS (1) IS8619A (no)
MX (1) MX2007002389A (no)
NO (1) NO344696B1 (no)
NZ (1) NZ553235A (no)
WO (1) WO2006029198A2 (no)

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