NO20071576L - Middel og kulturer av embryoniske stamceller - Google Patents

Middel og kulturer av embryoniske stamceller

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Publication number
NO20071576L
NO20071576L NO20071576A NO20071576A NO20071576L NO 20071576 L NO20071576 L NO 20071576L NO 20071576 A NO20071576 A NO 20071576A NO 20071576 A NO20071576 A NO 20071576A NO 20071576 L NO20071576 L NO 20071576L
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NO
Norway
Prior art keywords
stem cells
cells
embryonic stem
medium
fibroblast
Prior art date
Application number
NO20071576A
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English (en)
Other versions
NO344695B1 (no
Inventor
James A Thomson
Tenneille Ludwing
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Wisconsin Alumni Res Found
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Publication date
Application filed by Wisconsin Alumni Res Found filed Critical Wisconsin Alumni Res Found
Publication of NO20071576L publication Critical patent/NO20071576L/no
Publication of NO344695B1 publication Critical patent/NO344695B1/no

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N2500/00—Specific components of cell culture medium
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    • C12N2501/80—Neurotransmitters; Neurohormones
    • C12N2501/845—Gamma amino butyric acid [GABA]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Tidligere fremgangsmåter for dyrking av humane embryonale stamceller har krevd enten fibroblastnæringsceller eller et medium som er blitt eksponert for fibroblastnæringsceller for å opprettholde stamcellene i en udifferensiert tilstand. Det er nå fimnet at dersom høye nivåer av fibroblastvekstfaktor, gamma-aminosmørsyre, pipekolinsyre, litium og transformerende vekstfaktor beta tilsettes til mediet til hvilket stamcellen dyrkes, vil stamcellene bibeholde udifferensiert tilstand gjennom mange passasjer, selv uten næringsceller eller kondisjonert medium.
NO20071576A 2004-09-08 2007-03-26 Fremgangsmåte for dyrking av humane embryonale stamceller i en udifferensiert tilstand, in vitro cellekultur og medium for dyrkning av embryonale stamceller. NO344695B1 (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US60804004P 2004-09-08 2004-09-08
PCT/US2005/031838 WO2006029197A1 (en) 2004-09-08 2005-09-08 Medium and culture of embryonic stem cells

Publications (2)

Publication Number Publication Date
NO20071576L true NO20071576L (no) 2007-03-26
NO344695B1 NO344695B1 (no) 2020-03-09

Family

ID=35351611

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20071576A NO344695B1 (no) 2004-09-08 2007-03-26 Fremgangsmåte for dyrking av humane embryonale stamceller i en udifferensiert tilstand, in vitro cellekultur og medium for dyrkning av embryonale stamceller.

Country Status (17)

Country Link
US (5) US7449334B2 (no)
EP (1) EP1791948B1 (no)
JP (3) JP5420837B2 (no)
KR (1) KR101264940B1 (no)
CN (2) CN101052712A (no)
AT (1) ATE549395T1 (no)
AU (1) AU2005282510B2 (no)
BR (1) BRPI0514778B1 (no)
CA (1) CA2579643C (no)
ES (1) ES2383813T3 (no)
GB (1) GB2432846B (no)
IL (1) IL181437A (no)
IS (1) IS8618A (no)
MX (1) MX2007002390A (no)
NO (1) NO344695B1 (no)
NZ (1) NZ553241A (no)
WO (1) WO2006029197A1 (no)

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