NO302477B1 - Hovedsakelig ren, rekombinant humanendotelplasminogenaktivatorinhibitor, PAI-1, biologisk rent DNA-molekyl som koder for inhibitoren, samt ikke-kromosomal ekspresjonsvektor som koder for og kan uttrykke et PAI-1-gen - Google Patents

Hovedsakelig ren, rekombinant humanendotelplasminogenaktivatorinhibitor, PAI-1, biologisk rent DNA-molekyl som koder for inhibitoren, samt ikke-kromosomal ekspresjonsvektor som koder for og kan uttrykke et PAI-1-gen Download PDF

Info

Publication number: NO302477B1
Authority: NO; Norway
Prior art keywords: pai; inhibitor; sequence; protein; plasminogen activator
Prior art date: 1986-08-19

Application number

NO881670A

Other languages

English (en)

Norwegian (no)

Other versions

NO881670D0 (no

NO881670L (no

Inventor

David J Loskutoff

Tor Ny

Michael Sawdey

Original Assignee

Scripps Clinic Res

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1986-08-19

Filing date

1988-04-18

Publication date

1998-03-09

Family has litigation

First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25408764&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=NO302477(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

1988-04-18 Application filed by Scripps Clinic Res filed Critical Scripps Clinic Res

1988-04-18 Publication of NO881670D0 publication Critical patent/NO881670D0/no

1988-06-17 Publication of NO881670L publication Critical patent/NO881670L/no

1995-12-21 Priority to NO19955220A priority Critical patent/NO322046B1/no

1998-03-09 Publication of NO302477B1 publication Critical patent/NO302477B1/no

Links

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235000017557 sodium bicarbonate Nutrition 0.000 description 1
229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
239000007790 solid phase Substances 0.000 description 1
239000001593 sorbitan monooleate Substances 0.000 description 1
229940035049 sorbitan monooleate Drugs 0.000 description 1
239000007929 subcutaneous injection Substances 0.000 description 1
238000010254 subcutaneous injection Methods 0.000 description 1
239000005720 sucrose Substances 0.000 description 1
YBBRCQOCSYXUOC-UHFFFAOYSA-N sulfuryl dichloride Chemical compound ClS(Cl)(=O)=O YBBRCQOCSYXUOC-UHFFFAOYSA-N 0.000 description 1
238000003786 synthesis reaction Methods 0.000 description 1
230000008685 targeting Effects 0.000 description 1
238000002560 therapeutic procedure Methods 0.000 description 1
229960004072 thrombin Drugs 0.000 description 1
230000001732 thrombotic effect Effects 0.000 description 1
230000008354 tissue degradation Effects 0.000 description 1
230000007838 tissue remodeling Effects 0.000 description 1
230000002103 transcriptional effect Effects 0.000 description 1
238000001890 transfection Methods 0.000 description 1
238000003151 transfection method Methods 0.000 description 1
239000012581 transferrin Substances 0.000 description 1
QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
108010087967 type I signal peptidase Proteins 0.000 description 1
241001515965 unidentified phage Species 0.000 description 1
229940035893 uracil Drugs 0.000 description 1
210000004291 uterus Anatomy 0.000 description 1
230000003612 virological effect Effects 0.000 description 1
108010047303 von Willebrand Factor Proteins 0.000 description 1
102100036537 von Willebrand factor Human genes 0.000 description 1
238000007693 zone electrophoresis Methods 0.000 description 1

Classifications

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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
- C07K14/8121—Serpins
- C07K14/8132—Plasminogen activator inhibitors
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16611—Simplexvirus, e.g. human herpesvirus 1, 2
- C12N2710/16622—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/81—Protease inhibitors
- G01N2333/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- G01N2333/811—Serine protease (E.C. 3.4.21) inhibitors
- G01N2333/8121—Serpins
- G01N2333/8132—Plasminogen activator inhibitors
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/972—Plasminogen activators
- G01N2333/9723—Urokinase
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/972—Plasminogen activators
- G01N2333/9726—Tissue plasminogen activator

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Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
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Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Biophysics (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Medicinal Chemistry (AREA)
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Hematology (AREA)
Immunology (AREA)
Gastroenterology & Hepatology (AREA)
Urology & Nephrology (AREA)
Mycology (AREA)
Plant Pathology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Analytical Chemistry (AREA)
Pathology (AREA)
General Physics & Mathematics (AREA)
Cell Biology (AREA)
Virology (AREA)
Food Science & Technology (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)

NO881670A 1986-08-19 1988-04-18 Hovedsakelig ren, rekombinant humanendotelplasminogenaktivatorinhibitor, PAI-1, biologisk rent DNA-molekyl som koder for inhibitoren, samt ikke-kromosomal ekspresjonsvektor som koder for og kan uttrykke et PAI-1-gen NO302477B1 (no)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
NO19955220A NO322046B1 (no)	1986-08-19	1995-12-21	Fastfase-analysefremgangsmate for pavisning av endotelplasminogenaktivatorinhibitor

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US06/897,990 US4952512A (en)	1984-06-22	1986-08-19	Gene encoding an inhibitor of tissue-type and urokinase-type plasminogen activators
PCT/US1987/002053 WO1988001273A1 (en)	1986-08-19	1987-08-19	Diagnostic assay for inhibitor of tissue-type and urokinase-type plasminogen activators, and gene coding for the inhibitor

Publications (3)

Publication Number	Publication Date
NO881670D0 NO881670D0 (no)	1988-04-18
NO881670L NO881670L (no)	1988-06-17
NO302477B1 true NO302477B1 (no)	1998-03-09

Family

ID=25408764

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO881670A NO302477B1 (no)	1986-08-19	1988-04-18	Hovedsakelig ren, rekombinant humanendotelplasminogenaktivatorinhibitor, PAI-1, biologisk rent DNA-molekyl som koder for inhibitoren, samt ikke-kromosomal ekspresjonsvektor som koder for og kan uttrykke et PAI-1-gen

Country Status (10)

Country	Link
US (1)	US4952512A (da)
EP (1)	EP0277212B1 (da)
JP (2)	JP2619452B2 (da)
KR (1)	KR960005735B1 (da)
AT (1)	ATE120757T1 (da)
AU (1)	AU611095B2 (da)
DE (1)	DE3751224T2 (da)
DK (1)	DK175519B1 (da)
NO (1)	NO302477B1 (da)
WO (1)	WO1988001273A1 (da)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5422090A (en) *	1984-08-13	1995-06-06	Biotechnology Australia, Pty., Ltd.	Human PAI-2
US6291662B1 (en)	1984-12-05	2001-09-18	Amgen Inc.	Recombinant methods for production of serine protease inhibitors and DNA sequences
US5871956A (en) *	1984-12-06	1999-02-16	Amgen Inc.	Recombinant methods for production of serine inhibitors and DNA sequences useful for same
US6132990A (en) *	1984-12-06	2000-10-17	Amgen Boulder Inc.	Recombinant methods for production of serine protease inhibitors and DNA sequences useful for same
US5900400A (en) *	1984-12-06	1999-05-04	Amgen Inc.	Serine protease inhibitor analogs
US5853978A (en) *	1985-12-04	1998-12-29	Genentech, Inc.	Molecularly cloned acquired immunodeficiency syndrome polypeptides and methods of use
US6534285B1 (en)	1984-12-24	2003-03-18	Genentech, Inc.	Molecularly cloned acquired immunodeficiency syndrome polypeptides and their methods of use
US6271352B1 (en)	1985-07-12	2001-08-07	Fonden Til Fremme Af Eksperimentel Cancerforskning	PAI-1 determination and use thereof
US5648254A (en) *	1988-01-15	1997-07-15	Zymogenetics, Inc.	Co-expression in eukaryotic cells
SE8802939D0 (sv) *	1988-08-19	1988-08-19	Kabigen Ab	A method for the production and isolation of a fusion protein in eukaryotic cells
WO1990013648A1 (en) *	1989-05-11	1990-11-15	E.I. Du Pont De Nemours And Company	HIGH LEVEL EXPRESSION OF FUNCTIONAL HUMAN PLASMINOGEN ACTIVATOR INHIBITOR (PAI-1) IN $i(E. COLI)
WO1991002057A1 (en) *	1989-08-09	1991-02-21	Delta Biotechnology Limited	Polypeptide production in fungi
US5260419A (en) *	1991-03-20	1993-11-09	The Dupont Merck Pharmaceutical Company	Purification of active and inactive/latent forms of plasminogen activator inhibitor-1
US6869925B1 (en)	1992-09-09	2005-03-22	Amgen Inc.	Inhibition of retrovirus infection
US5474766A (en) *	1992-12-18	1995-12-12	Washington University	Methods and compositions for inhibition of hepatic clearance of tissue-type plasminogen activator
US5550213A (en) *	1993-12-27	1996-08-27	Rutgers, The State University Of New Jersey	Inhibitors of urokinase plasminogen activator
US6017880A (en) *	1994-03-09	2000-01-25	Amgen Inc.	Inhibition of retrovirus infection
US5639726A (en) *	1994-09-30	1997-06-17	The Regents Of The University Of Michigan	Peptide mediated enhancement of thrombolysis methods and compositions
DE29724847U1 (de) *	1996-06-26	2004-09-30	Osram Opto Semiconductors Gmbh	Lichtabstrahlendes Halbleiterbauelement mit Lumineszenzkonversionselement
US6600908B1 (en)	1999-02-04	2003-07-29	Hark C. Chan	Method and system for broadcasting and receiving audio information and associated audio indexes
DE602006003651D1 (de) *	2006-08-03	2008-12-24	Cellzome Ag	Verfahren zur Identifizierung von Molekülen die mit PI3K interagieren und Verfahren zur Reinigung von PI3K
US20140093556A1 (en)	2011-01-28	2014-04-03	Sanofi Pasteur Sa	Immunological Compositions Against HIV

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4235960A (en) *	1977-07-29	1980-11-25	The Medical College Of Wisconsin, Inc.	Competitive enzyme-linked immunoassay
US4766075A (en) *	1982-07-14	1988-08-23	Genentech, Inc.	Human tissue plasminogen activator
US4791068A (en) *	1984-06-22	1988-12-13	Scripps Clinic And Research Foundation	Diagnostic assay for inhibitor of tissue-type and urokinase-type plasminogen activators
DK319685A (da) *	1985-07-12	1987-01-13	Fonden Til Fremme Af Eksperime	Monoklonale antistoffer, fremgangsmaade til frembringelse af antistofferne, hybridomaceller, der producerer antistofferne, og anvendelse af antistofferne
NL8602307A (nl) *	1986-09-12	1988-04-05	Stichting Centraal Lab	Recombinant dna molekuul; werkwijze voor het bereiden van humane endotheliale plasminogeen activator remmer (pai) of een mutant daarvan; farmaceutisch preparaat; getransformeerde gastheercellen.
DE3713272A1 (de) *	1987-04-18	1988-11-03	Behringwerke Ag	Plasminogenaktivator-inhibitor, typ 2 (pai-2)

1986
- 1986-08-19 US US06/897,990 patent/US4952512A/en not_active Expired - Lifetime
1987
- 1987-08-19 AT AT87905549T patent/ATE120757T1/de not_active IP Right Cessation
- 1987-08-19 DE DE3751224T patent/DE3751224T2/de not_active Expired - Lifetime
- 1987-08-19 KR KR1019880700415A patent/KR960005735B1/ko not_active Expired - Lifetime
- 1987-08-19 AU AU78514/87A patent/AU611095B2/en not_active Expired
- 1987-08-19 JP JP62505083A patent/JP2619452B2/ja not_active Expired - Lifetime
- 1987-08-19 WO PCT/US1987/002053 patent/WO1988001273A1/en not_active Ceased
- 1987-08-19 EP EP87905549A patent/EP0277212B1/en not_active Expired - Lifetime
1988
- 1988-04-18 NO NO881670A patent/NO302477B1/no not_active IP Right Cessation
- 1988-04-18 DK DK198802107A patent/DK175519B1/da not_active IP Right Cessation
1996
- 1996-10-01 JP JP8260469A patent/JP2685742B2/ja not_active Expired - Lifetime

Also Published As

Publication number	Publication date
US4952512A (en)	1990-08-28
DK210788A (da)	1988-06-20
NO881670D0 (no)	1988-04-18
ATE120757T1 (de)	1995-04-15
AU611095B2 (en)	1991-06-06
DE3751224T2 (de)	1995-11-09
DK210788D0 (da)	1988-04-18
DK175519B1 (da)	2004-11-22
JPH09131192A (ja)	1997-05-20
EP0277212A4 (en)	1991-04-10
DE3751224D1 (de)	1995-05-11
JP2685742B2 (ja)	1997-12-03
EP0277212B1 (en)	1995-04-05
JPH01500640A (ja)	1989-03-09
NO881670L (no)	1988-06-17
KR960005735B1 (ko)	1996-05-01
WO1988001273A1 (en)	1988-02-25
EP0277212A1 (en)	1988-08-10
AU7851487A (en)	1988-03-08
JP2619452B2 (ja)	1997-06-11
KR880701728A (ko)	1988-11-04

Legal Events

Date	Code	Title	Description
2007-08-27	MK1K	Patent expired

Publication	Publication Date	Title
NO302477B1 (no)	1998-03-09	Hovedsakelig ren, rekombinant humanendotelplasminogenaktivatorinhibitor, PAI-1, biologisk rent DNA-molekyl som koder for inhibitoren, samt ikke-kromosomal ekspresjonsvektor som koder for og kan uttrykke et PAI-1-gen
Ny et al.	1986	Cloning and sequence of a cDNA coding for the human beta-migrating endothelial-cell-type plasminogen activator inhibitor.
van de Ven et al.	1990	Furin is a subtilisin-like proprotein processing enzyme in higher eukaryotes
EP1346034B1 (en)	2009-02-18	VON WILLEBRAND FACTOR (vWF) CLEAVING PROTEASE POLYPEPTIDE, NUCLEIC ACID ENCODING THE POLYPEPTIDE AND USE OF POLYPEPTIDE
CA1341485C (en)	2005-07-12	Fusion proteins utilizing the decay accelerating factor membrane binding domain
EP0187814B1 (en)	1992-09-16	Diagnostic assay for inhibitor or tissue-type and urokinase-type plasminogen activators
EP0352119A2 (en)	1990-01-24	Vampire bat salivary plasminogen activators
EP0918871A2 (en)	1999-06-02	Afc1 and rce1:isoprenylated caax processing enzymes
van Duijnhoven et al.	1992	Development and characterization of a panel of monoclonal antibodies against the novel subtilisin-like proprotein processing enzyme furin
JPH0640825B2 (ja)	1994-06-01	遺伝子操作による因子ＸＩＩＩａの製造
US5629160A (en)	1997-05-13	Diagnostic assay for inhibitor of tissue-type and urokinase-type plasminogen activators, and gene coding for the inhibitor
Tomomura et al.	1995	Molecular cloning and expression of serum calcium-decreasing factor (caldecrin)
NO322046B1 (no)	2006-08-07	Fastfase-analysefremgangsmate for pavisning av endotelplasminogenaktivatorinhibitor
Ny et al.	1989	Plasminogen activator inhibitor type 2 cDNA transfected into Chinese hamster ovary cells is stably expressed but not secreted
CA1341318C (en)	2001-11-13	Dna clones of human placental plasminogen activator inhibitor
Hofmann et al.	1992	Purification and characterisation of recombinant rabbit plasminogen activator inhibitor-1 expressed in Saccharomyces cerevisiae
Asselbergs et al.	1993	Localization of epitopes recognized by monoclonal antibodies on tissue-type and urokinase-type plasminogen activators using recombinant hybrid enzymes
JPH04505252A (ja)	1992-09-17	Ｅ．ｃｏｌｉにおける機能的ヒトプラスミノーゲン活性化因子阻害剤（ＰＡＩ―１）の高水準発現
ZA200304741B (en)	2004-05-06	Von willebrand factor (vWF) cleaving protease polypeptide, nucleic acid encoding the polypeptide and use of polypeptide.
JPH08187100A (ja)	1996-07-23	分泌蛋白質のｎ末端配列決定法