NO308610B1 - Ribozymer for anvendelse som midler for behandling av planter, fremgangsmÕte for fremstilling derav, og preparater omfattende slike ribozymer - Google Patents
Ribozymer for anvendelse som midler for behandling av planter, fremgangsmÕte for fremstilling derav, og preparater omfattende slike ribozymer Download PDFInfo
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- NO308610B1 NO308610B1 NO902661A NO902661A NO308610B1 NO 308610 B1 NO308610 B1 NO 308610B1 NO 902661 A NO902661 A NO 902661A NO 902661 A NO902661 A NO 902661A NO 308610 B1 NO308610 B1 NO 308610B1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
- A01N57/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/60—Isolated nucleic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
- C12N2310/121—Hammerhead
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
- C12N2310/124—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes based on group I or II introns
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Epidemiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Communicable Diseases (AREA)
- General Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Oncology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AUPI591187 | 1987-12-15 | ||
| AUPI995088 | 1988-08-19 | ||
| AUPJ035388 | 1988-09-09 | ||
| AUPJ130488 | 1988-11-04 | ||
| AUPJ133388 | 1988-11-07 | ||
| PCT/AU1988/000478 WO1989005852A1 (en) | 1987-12-15 | 1988-12-14 | Ribozymes |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO902661D0 NO902661D0 (no) | 1990-06-14 |
| NO902661L NO902661L (no) | 1990-08-14 |
| NO308610B1 true NO308610B1 (no) | 2000-10-02 |
Family
ID=27507391
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO902661A NO308610B1 (no) | 1987-12-15 | 1990-06-14 | Ribozymer for anvendelse som midler for behandling av planter, fremgangsmÕte for fremstilling derav, og preparater omfattende slike ribozymer |
| NO20000369A NO312105B1 (no) | 1987-12-15 | 2000-01-25 | Fremgangsmåte for fremstilling av ribozymer |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO20000369A NO312105B1 (no) | 1987-12-15 | 2000-01-25 | Fremgangsmåte for fremstilling av ribozymer |
Country Status (19)
| Country | Link |
|---|---|
| EP (2) | EP0321201B2 (de) |
| JP (1) | JP3046318B2 (de) |
| KR (1) | KR970010758B1 (de) |
| CN (1) | CN1102174C (de) |
| AR (1) | AR243935A1 (de) |
| AT (1) | ATE115999T1 (de) |
| AU (1) | AU632993B2 (de) |
| CA (1) | CA1340831C (de) |
| DE (1) | DE3852539T3 (de) |
| DK (1) | DK175956B1 (de) |
| ES (1) | ES2065919T5 (de) |
| FI (1) | FI104562B (de) |
| GR (1) | GR3015374T3 (de) |
| HU (2) | HUT54407A (de) |
| MC (1) | MC2115A1 (de) |
| NO (2) | NO308610B1 (de) |
| NZ (1) | NZ227332A (de) |
| RO (1) | RO114469B1 (de) |
| WO (1) | WO1989005852A1 (de) |
Families Citing this family (200)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5019556A (en) * | 1987-04-14 | 1991-05-28 | President And Fellows Of Harvard College | Inhibitors of angiogenin |
| CA1340323C (en) * | 1988-09-20 | 1999-01-19 | Arnold E. Hampel | Rna catalyst for cleaving specific rna sequences |
| US5866701A (en) * | 1988-09-20 | 1999-02-02 | The Board Of Regents For Northern Illinois University Of Dekalb | HIV targeted hairpin ribozymes |
| US5624824A (en) * | 1989-03-24 | 1997-04-29 | Yale University | Targeted cleavage of RNA using eukaryotic ribonuclease P and external guide sequence |
| US5168053A (en) * | 1989-03-24 | 1992-12-01 | Yale University | Cleavage of targeted RNA by RNAase P |
| DE4091533T (de) * | 1989-08-31 | 1992-01-30 | ||
| US5225347A (en) * | 1989-09-25 | 1993-07-06 | Innovir Laboratories, Inc. | Therapeutic ribozyme compositions and expression vectors |
| US5225337A (en) * | 1989-09-25 | 1993-07-06 | Innovir Laboratories, Inc. | Ribozyme compositions and methods for use |
| DE3933384A1 (de) * | 1989-10-06 | 1991-04-18 | Hoechst Ag | Multifunktionelle rna mit selbstprozessierungsaktivitaet, ihre herstellung und ihre verwendung |
| DE3935473A1 (de) * | 1989-10-25 | 1991-05-02 | Hoechst Ag | Rna mit endonuclease- und antisense-aktivitaet, ihre herstellung und ihre verwendung |
| HUT63170A (en) * | 1990-01-11 | 1993-07-28 | Isis Pharmaceuticals Inc | Process and composition for detecting and modifying rna activity and gene expression |
| DE4002885A1 (de) * | 1990-02-01 | 1991-08-08 | Hoechst Ag | Expression einer multigen rna mit self-splicing aktivitaet |
| US5519164A (en) * | 1990-02-01 | 1996-05-21 | Hoechst Aktiengesellschaft | Expression of a multigene RNA having self-splicing activity |
| US5180818A (en) * | 1990-03-21 | 1993-01-19 | The University Of Colorado Foundation, Inc. | Site specific cleavage of single-stranded dna |
| WO1991018913A1 (en) * | 1990-06-07 | 1991-12-12 | City Of Hope | Ribozyme mediated reversal of transformation by cleavage of the hras oncogene rna |
| AU7152191A (en) * | 1990-06-07 | 1991-12-31 | City Of Hope | Ribozyme mediated reversal of transformation by cleavage of the hras oncogene rna |
| US6008343A (en) * | 1990-06-19 | 1999-12-28 | Gene Shears Pty. Ltd. | Nucleotide based endonucleases |
| ATE150789T1 (de) * | 1990-06-19 | 1997-04-15 | Gene Shears Pty Ltd | Endonukleasen |
| US5246921A (en) * | 1990-06-26 | 1993-09-21 | The Wistar Institute Of Anatomy And Biology | Method for treating leukemias |
| WO1992000080A1 (en) * | 1990-06-26 | 1992-01-09 | The Wistar Institute Of Anatomy & Biology | Method for treating leukemias |
| JPH06502988A (ja) * | 1990-07-26 | 1994-04-07 | ファウンデーション・フォー・リサーチ・アンド・テクノロジー ― ヘラス(エフオー.アール.ティー.エイチ.)インスティテュート・オブ・モレキュラー・バイオロジー・アンド・バイオテクノロジー | ポータブルリボザイムカセット、それを含有するdna配列、これらdna配列によりコードされるリボザイム、およびこれらリボザイムを含有する組成物 |
| WO1992007065A1 (en) | 1990-10-12 | 1992-04-30 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Modified ribozymes |
| NZ241311A (en) * | 1991-01-17 | 1995-03-28 | Gen Hospital Corp | Rna sequence having trans-splicing activity, plant strains |
| NZ314629A (en) * | 1991-01-17 | 2000-08-25 | Gen Hospital Corp | Use trans-splicing ribozymes to prepare medicaments for gene therapies |
| FR2675803B1 (fr) | 1991-04-25 | 1996-09-06 | Genset Sa | Oligonucleotides fermes, antisens et sens et leurs applications. |
| FI913197A0 (fi) | 1991-07-01 | 1991-07-01 | Xyrofin Oy | Nya jaeststammar med reducerad foermaoga att metabolisera xylitol, foerfarande foer bildande av dessa och deras anvaendning vid framstaellning av xylitol. |
| JPH07502898A (ja) * | 1992-01-13 | 1995-03-30 | デューク・ユニバーシティー | 酵素rna分子 |
| AU656676B2 (en) * | 1992-04-17 | 1995-02-09 | Kirin Beer Kabushiki Kaisha | Plant resistant to two or more viruses and preparation thereof |
| ATE312510T1 (de) * | 1992-06-29 | 2005-12-15 | Gene Shears Pty Ltd | Nukleinsäuren und sie verwendende verfahren zur bekämpfung viraler pathogene |
| US5631115A (en) * | 1992-07-02 | 1997-05-20 | Sankyo Company, Limited | Looped, hairpin ribozyme |
| US5409823A (en) * | 1992-09-24 | 1995-04-25 | Ciba-Geigy Corporation | Methods for the production of hybrid seed |
| US5864028A (en) * | 1992-11-03 | 1999-01-26 | Gene Shears Pty. Limited | Degradation resistant mRNA derivatives linked to TNF-α ribozymes |
| ZA938208B (en) * | 1992-11-03 | 1995-01-03 | Gene Shears Pty Ltd | TNF-alpha ribozymes and degradation resistant mRNA derivatives linked to TNF-alpha ribozymes |
| WO1994013793A1 (en) * | 1992-12-04 | 1994-06-23 | Apollon, Inc. | Compounds and methods for the treatment of leukemias |
| WO1994013791A1 (en) * | 1992-12-04 | 1994-06-23 | Innovir Laboratories, Inc. | Regulatable nucleic acid therapeutic and methods of use thereof |
| JP3595841B2 (ja) * | 1992-12-04 | 2004-12-02 | サーナ セラピューティクス,インコーポレイテッド | リボザイム増幅診断用薬 |
| FR2701960B1 (fr) * | 1993-02-26 | 2002-09-13 | Gene Shears Pty Ltd | Polyribozyme apte à conférer, aux plantes, une résistance aux virus et plantes résistantes produisant ce polyribozyme. |
| US5817635A (en) * | 1993-08-09 | 1998-10-06 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Modified ribozymes |
| AU5334294A (en) * | 1993-10-15 | 1995-05-04 | Dkfz Deutsches Krebsforschungszentrum Stiftung Des Offentlichen Rechts | Asymmetric hammerhead ribozymes and nucleotide sequences for their construction |
| US5869248A (en) * | 1994-03-07 | 1999-02-09 | Yale University | Targeted cleavage of RNA using ribonuclease P targeting and cleavage sequences |
| US5998193A (en) * | 1994-06-24 | 1999-12-07 | Gene Shears Pty., Ltd. | Ribozymes with optimized hybridizing arms, stems, and loops, tRNA embedded ribozymes and compositions thereof |
| US6350934B1 (en) | 1994-09-02 | 2002-02-26 | Ribozyme Pharmaceuticals, Inc. | Nucleic acid encoding delta-9 desaturase |
| US6057153A (en) * | 1995-01-13 | 2000-05-02 | Yale University | Stabilized external guide sequences |
| US5683873A (en) * | 1995-01-13 | 1997-11-04 | Innovir Laboratories, Inc. | EGS-mediated inactivation of target RNA |
| FR2730637B1 (fr) | 1995-02-17 | 1997-03-28 | Rhone Poulenc Rorer Sa | Composition pharmaceutique contenant des acides nucleiques, et ses utilisations |
| EP0837933A4 (de) * | 1995-06-07 | 2003-05-21 | Commw Scient Ind Res Org | Optimierte minizyme und miniribozyme und deren anwendungen |
| US6004806A (en) * | 1995-06-07 | 1999-12-21 | Commonwealth Scientific And Industrial Research Organization | Optimized minizymes and miniribozymes and uses thereof |
| US6010904A (en) * | 1995-06-07 | 2000-01-04 | The General Hospital Corporation | Cell ablation using trans-splicing ribozymes |
| WO1997010328A2 (en) * | 1995-07-13 | 1997-03-20 | Ribozyme Pharmaceuticals, Inc. | Compositions and method for modulation of gene expression in plants |
| US5824519A (en) * | 1995-11-08 | 1998-10-20 | Medical University Of South Carolina | Tissue-specific and target RNA-specific ribozymes |
| US5877162A (en) * | 1996-03-14 | 1999-03-02 | Innovir Laboratories, Inc. | Short external guide sequences |
| US6620805B1 (en) | 1996-03-14 | 2003-09-16 | Yale University | Delivery of nucleic acids by porphyrins |
| US5976874A (en) * | 1996-08-16 | 1999-11-02 | Yale University | Phenotypic conversion of drug-resistant bacteria to drug-sensitivity |
| US6610478B1 (en) | 1996-08-16 | 2003-08-26 | Yale University | Phenotypic conversion of cells mediated by external guide sequences |
| US6884430B1 (en) | 1997-02-10 | 2005-04-26 | Aventis Pharma S.A. | Formulation of stabilized cationic transfection agent(s) /nucleic acid particles |
| US6969601B2 (en) | 1997-04-03 | 2005-11-29 | Jensenius Jens Chr | MASP-2, a complement-fixing enzyme, and uses for it |
| US7589253B2 (en) | 1997-04-15 | 2009-09-15 | Commonwealth Scientific And Industrial Research Organisation | Fatty acid epoxygenase genes from plants and uses therefor in modifying fatty acid metabolism |
| PL194095B1 (pl) | 1997-04-15 | 2007-04-30 | Commw Scient Ind Res Org | Wyizolowana cząsteczka kwasu nukleinowego, konstrukt genetyczny, sposób dokonywania zmian poziomu epoksykwasów tłuszczowych, sposób wytwarzania rekombinantowego polipeptydu monooksygenazy w komórce, sposób wytwarzania rekombinantowego polipeptydu monooksygenazy w roślinie transgenicznej, polipeptydrekombinantowy, sposób wytwarzania epoksydowanegokwasu tłuszczowego, roślina transformowana i roślina lub pochodząca z niej komórka |
| DE69826124T3 (de) | 1997-06-30 | 2007-10-11 | Institut Gustave Roussy | Verabreichung der nukleinsäure in den quergestreiften muskel |
| JP2001516582A (ja) | 1997-09-16 | 2001-10-02 | クロップデザイン エヌ.ブイ. | サイクリン依存性キナーゼ阻害物質およびその使用 |
| DE19741375C2 (de) * | 1997-09-19 | 1999-10-21 | Max Planck Gesellschaft | Transgene Pflanzen, deren oberirdische Teile früher reifen und vollständig absterben |
| WO1999017798A1 (en) | 1997-10-02 | 1999-04-15 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Methods for the modulation of neovascularization and/or the growth of collateral arteries and/or other arteries from preexisting arteriolar connections |
| JP4749541B2 (ja) | 1997-11-18 | 2011-08-17 | マックス−プランク−ゲゼルシャフト・ツア・フェルデルング・デア・ヴィッセンシャフテン・エー・ファオ | レスポンダー表現型に関わる核酸およびその適用 |
| US6013447A (en) * | 1997-11-21 | 2000-01-11 | Innovir Laboratories, Inc. | Random intracellular method for obtaining optimally active nucleic acid molecules |
| AUPP249298A0 (en) | 1998-03-20 | 1998-04-23 | Ag-Gene Australia Limited | Synthetic genes and genetic constructs comprising same I |
| BRPI9908967B1 (pt) | 1998-03-20 | 2017-05-30 | Benitec Australia Ltd | processos para reprimir, retardar ou de outro modo reduzir a expressão de um gene alvo em uma célula de planta |
| US6248525B1 (en) | 1998-03-30 | 2001-06-19 | Yale University | Method for identifying essential or functional genes |
| AU4830899A (en) | 1998-06-24 | 2000-01-10 | Musc Foundation For Research Development | Tissue-specific and target rna-specific ribozymes |
| DE19836098A1 (de) | 1998-07-31 | 2000-02-03 | Hoechst Schering Agrevo Gmbh | Pflanzen, die eine modifizierte Stärke synthetisieren, Verfahren zur Herstellung der Pflanzen, ihre Verwendung sowie die modifizierte Stärke |
| JP2002527066A (ja) | 1998-10-15 | 2002-08-27 | カイロン コーポレイション | 転移性乳癌および結腸癌調節遺伝子 |
| CN100408687C (zh) * | 1998-11-09 | 2008-08-06 | 拜尔生物科学有限公司 | 来自稻米的核酸分子及其用于生产改性淀粉的用途 |
| TR200101342T2 (tr) | 1998-11-12 | 2001-09-21 | Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften | Patojen enfeksiyonu üzerine bitkilerde gen ekspresyonuna aracılık edebilen şimerik promoterler |
| EP1961813B1 (de) | 1998-12-16 | 2011-08-10 | Novartis Vaccines and Diagnostics, Inc. | Menschliche Cyclin-abhängige Kinase (hPNQALRE) |
| US6271359B1 (en) | 1999-04-14 | 2001-08-07 | Musc Foundation For Research Development | Tissue-specific and pathogen-specific toxic agents and ribozymes |
| CA2365901A1 (en) | 1999-04-14 | 2000-10-19 | Musc Foundation For Research Development | Tissue-specific and pathogen-specific toxic agents and ribozymes |
| AUPQ005299A0 (en) | 1999-04-29 | 1999-05-27 | Commonwealth Scientific And Industrial Research Organisation | Novel genes encoding wheat starch synthases and uses therefor |
| ATE305718T1 (de) | 1999-07-20 | 2005-10-15 | Gvs Ges Fuer Erwerb Und Verwer | Neues verfahren zur herstellung und auswahl von transgenen flachspflanzen und leinsamen |
| US6423885B1 (en) | 1999-08-13 | 2002-07-23 | Commonwealth Scientific And Industrial Research Organization (Csiro) | Methods for obtaining modified phenotypes in plant cells |
| JP5610659B2 (ja) | 2000-07-21 | 2014-10-22 | ルバンス セラピュティックス インク.Revance Therapeutics,Inc. | 多成分生物学的輸送システム |
| CN1482856A (zh) | 2000-11-09 | 2004-03-17 | ����Ƽ���ҵ�о���֯ | 降低了ssii活性的大麦和降低了支链淀粉含量的淀粉和淀粉制品 |
| ITMI20011364A1 (it) * | 2001-06-28 | 2002-12-28 | Metapontum Agrobios S C R L | Robozima hammerhead specifico per la stearoyl-acp desaturesi di piante oleaginose differenti |
| US7994144B2 (en) | 2001-07-10 | 2011-08-09 | Johnson & Johnson Research Pty, Limited | Process for the preparation of a composition of genetically modified hematopoietic progenitor cells |
| CN1553963A (zh) | 2001-07-10 | 2004-12-08 | ǿ����ǿ���о�����˾ | 用于遗传修饰造血祖细胞的方法和这些被修饰细胞的应用 |
| US8022272B2 (en) | 2001-07-13 | 2011-09-20 | Sungene Gmbh & Co. Kgaa | Expression cassettes for transgenic expression of nucleic acids |
| FR2829136B1 (fr) | 2001-08-29 | 2006-11-17 | Aventis Pharma Sa | Derives lipidiques d'aminoglycosides |
| US7456335B2 (en) | 2001-09-03 | 2008-11-25 | Basf Plant Science Gmbh | Nucleic acid sequences and their use in methods for achieving pathogen resistance in plants |
| EP2284195A1 (de) | 2001-09-18 | 2011-02-16 | Carnegie Institution Of Washington | Fusionsproteine zur Detektion von Analyten |
| AU2002359761A1 (en) | 2001-12-18 | 2003-06-30 | Invenux, Inc. | Antibiotic compounds |
| US7432371B2 (en) | 2002-02-07 | 2008-10-07 | Covalent Partners, Llc | Nanofilm and membrane compositions |
| US20040005546A1 (en) | 2002-02-28 | 2004-01-08 | Oncolytics Biotech Inc. | Use of ribozymes in the detection of adventitious agents |
| DE10212892A1 (de) | 2002-03-20 | 2003-10-09 | Basf Plant Science Gmbh | Konstrukte und Verfahren zur Regulation der Genexpression |
| EP1365034A3 (de) | 2002-05-21 | 2004-02-18 | Bayer HealthCare AG | Verfahren und Zusammensetzungen zur Vorhersage, Diagnose, Prognose, Prävention und Behandlung von malignen Tumoren |
| US7771952B2 (en) | 2002-06-26 | 2010-08-10 | Abott Laboratories | Modulators and modulation of the interaction between RGM and Neogenin |
| ATE546539T1 (de) | 2002-07-26 | 2012-03-15 | Basf Plant Science Gmbh | Revertierung der negativ-selektiven wirkung von negativen markerproteinen als selektionsverfahren |
| EP2258724A1 (de) | 2002-11-21 | 2010-12-08 | Celltech R & D, Inc. | Modulierung von Immunantworten mittels multimeriserter Anti-CD83 Antikörper |
| DE60323149D1 (de) | 2002-12-19 | 2008-10-02 | Bayer Cropscience Gmbh | Pflanzenzellen und pflanzen, die eine stärke mit erhöhter endviskosität synthetisieren |
| CN1798769A (zh) | 2003-05-12 | 2006-07-05 | 内蒂穆恩公司 | 抗masp-2抗体 |
| WO2005001098A1 (en) | 2003-06-30 | 2005-01-06 | Commonwealth Scientific And Industrial Research Organisation | Wheat with altered branching enzyme activity and starch and starch containing products derived thereform |
| EP1892306A3 (de) | 2003-10-06 | 2008-06-11 | Bayer HealthCare AG | Verfahren und Kit zur Untersuchung von Krebs |
| EP1602926A1 (de) | 2004-06-04 | 2005-12-07 | University of Geneva | Neue Mittel und Verfahren zur Behandlung von Gehörverlust oder Tinnitus |
| US7604947B2 (en) | 2004-06-09 | 2009-10-20 | Cornell Research Foundation, Inc. | Detection and modulation of cancer stem cells |
| EP2386315A1 (de) | 2004-06-10 | 2011-11-16 | Omeros Corporation | Verfahren zur Behandlung von Leiden in Zusammenhang mit MASP-2-abhängiger Komplementaktivierung |
| US7919094B2 (en) | 2004-06-10 | 2011-04-05 | Omeros Corporation | Methods for treating conditions associated with MASP-2 dependent complement activation |
| US8840893B2 (en) | 2004-06-10 | 2014-09-23 | Omeros Corporation | Methods for treating conditions associated with MASP-2 dependent complement activation |
| EP1763582B1 (de) | 2004-07-08 | 2014-12-10 | DLF - Trifolium A/S | Mittel und Verfahren zur Steuerung der Blüte in Pflanzen |
| EP1781784A2 (de) | 2004-08-02 | 2007-05-09 | BASF Plant Science GmbH | Verfahren zur isolierung von transkriptionsterminationssequenzen |
| AU2005286427B2 (en) | 2004-09-24 | 2011-09-15 | Basf Plant Science Gmbh | Plant cells and plants with increased tolerance to environmental stress |
| WO2006069422A1 (en) | 2004-12-30 | 2006-07-06 | Commonwealth Scientific And Industrial Research Organisation | Method and means for improving bowel health |
| EP1707632A1 (de) | 2005-04-01 | 2006-10-04 | Bayer CropScience GmbH | Phosphorylierte waxy-Kartoffelstärke |
| CN101277974A (zh) | 2005-09-30 | 2008-10-01 | 阿伯特有限及两合公司 | 排斥性引导分子(rgm)蛋白质家族的蛋白质的结合结构域及其功能性片段和它们的用途 |
| CA2628505A1 (en) | 2005-11-08 | 2007-05-18 | Basf Plant Science Gmbh | Use of armadillo repeat (arm1) polynucleotides for obtaining resistance to pathogens in plants |
| CA2632872A1 (en) | 2006-01-12 | 2007-07-19 | Basf Plant Science Gmbh | Use of stomatin (stm1) polynucleotides for achieving a pathogen resistance in plants |
| CA2644273A1 (en) | 2006-04-05 | 2008-03-27 | Metanomics Gmbh | Process for the production of a fine chemical |
| EP2030021B1 (de) | 2006-05-18 | 2012-11-21 | Andreas Reichert | Verfahren zur diagnose mitochondrialer fehlfunktion |
| US8383597B2 (en) | 2006-05-25 | 2013-02-26 | Cornell Research Foundation, Inc. | G proteins in tumor growth and angiogenesis |
| US9127311B2 (en) | 2006-10-06 | 2015-09-08 | Speedx Pty Ltd | Molecular switches and methods for their use |
| DK2202314T3 (da) | 2007-01-15 | 2014-06-16 | Basf Plant Science Gmbh | Anvendelse af subtilisin (RNR9)-polynukleotider til opnåelse af patogenresistens i planter |
| CA2675926A1 (en) | 2007-02-16 | 2008-08-21 | Basf Plant Science Gmbh | Nucleic acid sequences for regulation of embryo-specific expression in monocotyledonous plants |
| WO2008119057A2 (en) | 2007-03-27 | 2008-10-02 | Omeros Corporation | The use of pde7 inhibitors for the treatment of movement disorders |
| US20100162432A1 (en) | 2007-05-22 | 2010-06-24 | Basf Plant Science Gmbh | Plant cells and plants with increased tolerance and/or resistance to environmental stress and increased biomass production-ko |
| CL2008002775A1 (es) | 2007-09-17 | 2008-11-07 | Amgen Inc | Uso de un agente de unión a esclerostina para inhibir la resorción ósea. |
| EP2040075A1 (de) | 2007-09-24 | 2009-03-25 | Julius-Maximilians-Universität Würzburg | Verbindungen und Marker für oberflächenverstärkte Raman-Streuung |
| JP2011506483A (ja) | 2007-12-14 | 2011-03-03 | アムジエン・インコーポレーテツド | 抗スクレロスチン抗体を用いた骨折の治療方法 |
| US20100293665A1 (en) | 2007-12-21 | 2010-11-18 | Basf Plant Science Gmbh | Plants With Increased Yield (KO NUE) |
| US8962803B2 (en) | 2008-02-29 | 2015-02-24 | AbbVie Deutschland GmbH & Co. KG | Antibodies against the RGM A protein and uses thereof |
| EP2100962A1 (de) | 2008-03-12 | 2009-09-16 | Biogemma | Pflanzen mit verbesserter Widerstandsfähigkeit gegen Pathogene |
| US10517839B2 (en) | 2008-06-09 | 2019-12-31 | Cornell University | Mast cell inhibition in diseases of the retina and vitreous |
| WO2010026537A1 (en) | 2008-09-05 | 2010-03-11 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Novel multimodular assembly useful for intracellular delivery |
| EP2184351A1 (de) | 2008-10-30 | 2010-05-12 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Polynukleotid-kondierende Caryophyllen-Synthase und ihre Verwendung |
| ES2363358B1 (es) | 2009-04-03 | 2012-06-21 | FUNDACIÓ INSTITUT DE RECERCA HOSPITAL UNIVERSITARI VALL D'HEBRON (Titular al | Agentes terapéuticos para el tratamiento de enfermedades asociadas con una proliferación celular indeseable. |
| CA3020943A1 (en) | 2009-04-22 | 2010-10-28 | Basf Plant Science Company Gmbh | Whole seed specific promoter |
| EP2475367A1 (de) | 2009-09-10 | 2012-07-18 | Centre National De La Recherche Scientifique | Neuartige stearoyl-coa-desaturase-1-hemmer und ihre verwendung |
| EP2305285A1 (de) | 2009-09-29 | 2011-04-06 | Julius-Maximilians-Universität Würzburg | Mittel und Verfahren zur Behandlung von ischämischen Erkrankungen |
| AU2010306581B2 (en) | 2009-10-16 | 2015-03-19 | Omeros Corporation | Methods for treating disseminated intravascular coagulation by inhibiting MASP-2 dependent complement activation |
| WO2011051478A1 (en) | 2009-10-30 | 2011-05-05 | Domain Therapeutics | Novel oxime derivatives and their use as allosteric modulators of metabotropic glutamate receptors |
| EP2322149A1 (de) | 2009-11-03 | 2011-05-18 | Universidad del Pais Vasco | Verfahren und Zusammensetzung zur Behandlung von Ischämie |
| KR20120118002A (ko) | 2009-12-08 | 2012-10-25 | 애보트 게엠베하 운트 콤파니 카게 | 망막 신경 섬유 층 변성의 치료에 사용하기 위한 rgm a 단백질에 대한 모노클로날 항체 |
| EP2338492A1 (de) | 2009-12-24 | 2011-06-29 | Universidad del Pais Vasco | Verfahren und Zusammensetzung zur Behandlung von Alzheimer |
| US8476485B2 (en) | 2010-06-24 | 2013-07-02 | Academia Sinica | Non-human animal model for amyotrophic lateral sclerosis (ALS) with loss-of-TDP-43 function |
| KR20130124333A (ko) | 2010-11-08 | 2013-11-13 | 오메로스 코포레이션 | Pde7 억제제를 사용한 중독 및 충동-조절 장애의 치료 |
| US9220715B2 (en) | 2010-11-08 | 2015-12-29 | Omeros Corporation | Treatment of addiction and impulse-control disorders using PDE7 inhibitors |
| US9644035B2 (en) | 2011-04-08 | 2017-05-09 | Omeros Corporation | Methods for treating conditions associated with MASP-2 dependent complement activation |
| CN107638565B (zh) | 2011-04-08 | 2021-12-21 | 莱斯特大学 | 用于治疗与masp-2依赖性补体活化相关的状况的方法 |
| WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| WO2013025936A1 (en) | 2011-08-18 | 2013-02-21 | Cornell University | Detection and treatment of metastatic disease |
| WO2013039880A1 (en) | 2011-09-12 | 2013-03-21 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Biomarkers and therapeutic targets of hepatocellular cancer |
| CA2859564C (en) | 2011-12-16 | 2020-04-14 | Board Of Trustees Of Michigan State University | P-coumaroyl-coa:monolignol transferase |
| EP3369746A1 (de) | 2012-01-27 | 2018-09-05 | AbbVie Deutschland GmbH & Co KG | Zusammensetzung und verfahren zur diagnose und behandlung von krankheiten im zusammenhang mit neuritenabbau |
| WO2013138456A1 (en) | 2012-03-14 | 2013-09-19 | University Of Central Florida Research Foundation, Inc. | Lim kinasemodulating agents for neurofibromatoses therapy and methods for screening for same |
| EP2666775A1 (de) | 2012-05-21 | 2013-11-27 | Domain Therapeutics | Substituierte Pyrazolochinazolinone und Pyrrolochinazolinone als allosterische Modulatoren von metabotropen Glutamatrezeptoren der Gruppe II |
| WO2014127835A1 (en) | 2013-02-22 | 2014-08-28 | Christian-Albrechts-Universität Zu Kiel | Plant-derived resistance gene |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| RS61351B1 (sr) | 2013-10-17 | 2021-02-26 | Omeros Corp | Postupci za lečenje stanja udruženih sa aktivacijom komplementa zavisnom od masp-2 |
| US20150166984A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting alpha-antitrypsin point mutations |
| PE20170253A1 (es) | 2013-12-18 | 2017-04-14 | Nuseed Pty Ltd | Lipido que comprende acidos grasos poliinsaturados de cadena larga |
| AU2015281791A1 (en) | 2014-06-27 | 2017-01-12 | Commonwealth Scientific And Industrial Research Organisation | Lipid comprising docosapentaenoic acid |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| EP3000814A1 (de) | 2014-09-26 | 2016-03-30 | Domain Therapeutics | Substituierte Pyrazolochinazolinone und Pyrrolochinazolinone als allosterische Modulatoren von metabotropen Glutamatrezeptoren der Gruppe II |
| SG10202104041PA (en) | 2015-10-23 | 2021-06-29 | Harvard College | Nucleobase editors and uses thereof |
| EA201891132A1 (ru) | 2015-11-09 | 2018-10-31 | Омерос Корпорейшн | Способы лечения состояний, связанных с masp-2 зависимой активацией комплемента |
| SG11201805695SA (en) | 2016-01-05 | 2018-07-30 | Univ Leicester | Methods for inhibiting fibrosis in a subject in need thereof |
| EA202191185A1 (ru) | 2016-03-31 | 2021-10-29 | Омерос Корпорейшн | Способы ингибирования ангиогенеза у пациента |
| WO2017202715A1 (en) | 2016-05-26 | 2017-11-30 | Nunhems B.V. | Seedless fruit producing plants |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| FI3565891T3 (fi) | 2017-01-09 | 2023-07-04 | Whitehead Inst Biomedical Res | Menetelmiä geeniekspression muuttamiseksi häiritsemällä säätelysilmukoita muodostavia transkriptiotekijämultimeerejä |
| US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| EP3592381A1 (de) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Krebsimpfstoff |
| WO2018165629A1 (en) | 2017-03-10 | 2018-09-13 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| US10883089B2 (en) | 2017-04-04 | 2021-01-05 | Wisconsin Alumni Research Foundation | Feruloyl-CoA:monolignol transferases |
| US10883090B2 (en) | 2017-04-18 | 2021-01-05 | Wisconsin Alumni Research Foundation | P-coumaroyl-CoA:monolignol transferases |
| JP7598137B2 (ja) | 2017-07-17 | 2024-12-11 | ザ・リージエンツ・オブ・ザ・ユニバーシテイ・オブ・コロラド、ア・ボデイー・コーポレイト | 放射線または放射線療法によって引き起こされた放射線誘発性バイスタンダ効果を防止および治療するための組成物および方法 |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| TW202529812A (zh) | 2017-08-15 | 2025-08-01 | 美商歐米諾斯公司 | 用於治療和/或預防與造血幹細胞移植有關的移植物抗宿主病和/或瀰漫性肺泡出血和/或靜脈閉塞性病的方法 |
| EP3676376B1 (de) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | Hocheffiziente baseneditoren mit gam |
| US11795443B2 (en) | 2017-10-16 | 2023-10-24 | The Broad Institute, Inc. | Uses of adenosine base editors |
| WO2019118949A1 (en) | 2017-12-15 | 2019-06-20 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| CN118373875A (zh) | 2018-05-29 | 2024-07-23 | 奥默罗斯公司 | Masp-2抑制剂和使用方法 |
| US11584714B2 (en) | 2018-05-29 | 2023-02-21 | Omeros Corporation | MASP-2 inhibitors and methods of use |
| US11291176B2 (en) | 2018-06-15 | 2022-04-05 | Nunhems B.V. | Seedless watermelon plants comprising modifications in an ABC transporter gene |
| EP3820495A4 (de) | 2018-07-09 | 2022-07-20 | The Broad Institute Inc. | Rna-programmierbare epigenetische rna-modifikatoren und deren verwendungen |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US11981904B2 (en) | 2018-11-09 | 2024-05-14 | Wisconsin Alumni Research Foundation | BAHD acyltransferases |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| EP3942043A2 (de) | 2019-03-19 | 2022-01-26 | The Broad Institute, Inc. | Verfahren und zusammensetzungen zur editierung von nukleotidsequenzen |
| WO2020214842A1 (en) | 2019-04-17 | 2020-10-22 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| KR20220110531A (ko) | 2019-12-04 | 2022-08-08 | 오메로스 코포레이션 | Masp-2 억제자 및 사용 방법 |
| WO2021113698A1 (en) | 2019-12-04 | 2021-06-10 | Omeros Corporation | Masp-2 inhibitors and methods of use |
| WO2021113682A1 (en) | 2019-12-04 | 2021-06-10 | Omeros Corporation | Masp-2 inhibitors and methods of use |
| TWI867422B (zh) | 2020-03-06 | 2024-12-21 | 美商奥默羅斯公司 | 用於治療和/或預防流感病毒誘導的急性呼吸窘迫症候群、肺炎或者流感病毒感染的一些其它肺部表現的抑制masp-2的方法 |
| BR112022022603A2 (pt) | 2020-05-08 | 2023-01-17 | Broad Inst Inc | Métodos e composições para edição simultânea de ambas as fitas de sequência alvo de nucleotídeos de fita dupla |
| CN116096378A (zh) | 2020-08-10 | 2023-05-09 | 诺华股份有限公司 | 视网膜变性疾病的治疗 |
| AU2023326563A1 (en) | 2022-08-18 | 2025-02-27 | Mitodicure Gmbh | Use of a therapeutic agent with phosphodiesterase-7 inhibitory activity for the treatment and prevention of diseases associated with chronic fatigue, exhaustion and/or exertional intolerance |
| CN119923254A (zh) | 2022-08-26 | 2025-05-02 | 米托迪治愈有限责任公司 | 具有钠氢反向转运物1抑制活性的治疗剂用于治疗和预防与慢性疲劳、筋疲力尽和/或劳累不耐受相关的疾病的用途 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4987071A (en) * | 1986-12-03 | 1991-01-22 | University Patents, Inc. | RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods |
| US5116742A (en) * | 1986-12-03 | 1992-05-26 | University Patents, Inc. | RNA ribozyme restriction endoribonucleases and methods |
| DE3642623A1 (de) * | 1986-12-13 | 1988-06-23 | Burbach & Bender Ohg | Gasspuelstein fuer metallurgische gefaesse |
| DE3939771A1 (de) * | 1989-12-01 | 1991-06-06 | Behringwerke Ag | Verfahren zur biologischen inaktivierung von nukleinsaeuren |
-
1988
- 1988-12-14 AU AU28007/89A patent/AU632993B2/en not_active Expired
- 1988-12-14 AR AR88312737A patent/AR243935A1/es active
- 1988-12-14 EP EP88311816A patent/EP0321201B2/de not_active Expired - Lifetime
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- 1988-12-14 CA CA000585845A patent/CA1340831C/en not_active Expired - Fee Related
- 1988-12-14 NZ NZ227332A patent/NZ227332A/xx unknown
- 1988-12-14 JP JP1500279A patent/JP3046318B2/ja not_active Expired - Lifetime
- 1988-12-14 RO RO145344A patent/RO114469B1/ro unknown
- 1988-12-14 MC MC882115D patent/MC2115A1/xx unknown
- 1988-12-14 EP EP94107862A patent/EP0640688A1/de not_active Withdrawn
- 1988-12-14 ES ES88311816T patent/ES2065919T5/es not_active Expired - Lifetime
- 1988-12-14 WO PCT/AU1988/000478 patent/WO1989005852A1/en not_active Ceased
- 1988-12-14 HU HU89382A patent/HUT54407A/hu unknown
- 1988-12-14 KR KR1019890701528A patent/KR970010758B1/ko not_active Expired - Fee Related
- 1988-12-14 AT AT88311816T patent/ATE115999T1/de not_active IP Right Cessation
- 1988-12-15 CN CN88108572A patent/CN1102174C/zh not_active Expired - Lifetime
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1990
- 1990-06-12 DK DK199001433A patent/DK175956B1/da not_active IP Right Cessation
- 1990-06-12 FI FI902923A patent/FI104562B/fi not_active IP Right Cessation
- 1990-06-14 NO NO902661A patent/NO308610B1/no unknown
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1995
- 1995-03-13 GR GR940403895T patent/GR3015374T3/el unknown
- 1995-06-22 HU HU95P/P00362P patent/HU211891A9/hu unknown
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2000
- 2000-01-25 NO NO20000369A patent/NO312105B1/no unknown
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