NO326180B1 - Medium for dyrking av dyreceller og fremgangsmate for fremstilling av protein ved anvendelse av de samme - Google Patents

Medium for dyrking av dyreceller og fremgangsmate for fremstilling av protein ved anvendelse av de samme Download PDF

Info

Publication number: NO326180B1
Authority: NO; Norway
Prior art keywords: culture medium; animal cell; fish meat; growing; stated
Prior art date: 1998-06-01

Application number

NO20006096A

Other languages

English (en)

Norwegian (no)

Other versions

NO20006096L (no

NO20006096D0 (no

Inventor

Kazushi Shibuya

Masaru Atsumi

Shigeyuki Tsunakawa

Kaneo Nogaki

Original Assignee

Chugai Pharmaceutical Co Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1998-06-01

Filing date

2000-11-30

Publication date

2008-10-13

2000-11-30 Application filed by Chugai Pharmaceutical Co Ltd filed Critical Chugai Pharmaceutical Co Ltd

2000-11-30 Publication of NO20006096D0 publication Critical patent/NO20006096D0/no

2001-02-01 Publication of NO20006096L publication Critical patent/NO20006096L/no

2008-10-13 Publication of NO326180B1 publication Critical patent/NO326180B1/no

Links

210000004102 animal cell Anatomy 0.000 title claims abstract description 53
108090000623 proteins and genes Proteins 0.000 title claims abstract description 49
102000004169 proteins and genes Human genes 0.000 title claims abstract description 40
238000000034 method Methods 0.000 title description 10
238000012258 culturing Methods 0.000 title 1
239000001963 growth medium Substances 0.000 claims abstract description 80
241000251468 Actinopterygii Species 0.000 claims abstract description 73
235000013372 meat Nutrition 0.000 claims abstract description 70
239000007857 degradation product Substances 0.000 claims abstract description 25
230000007515 enzymatic degradation Effects 0.000 claims abstract description 22
238000004519 manufacturing process Methods 0.000 claims abstract description 18
235000019688 fish Nutrition 0.000 claims description 71
108091005804 Peptidases Proteins 0.000 claims description 21
239000000047 product Substances 0.000 claims description 20
102000035195 Peptidases Human genes 0.000 claims description 19
210000004978 chinese hamster ovary cell Anatomy 0.000 claims description 17
239000002609 medium Substances 0.000 claims description 17
230000015556 catabolic process Effects 0.000 claims description 16
230000002255 enzymatic effect Effects 0.000 claims description 16
235000019833 protease Nutrition 0.000 claims description 14
239000004365 Protease Substances 0.000 claims description 12
235000019512 sardine Nutrition 0.000 claims description 10
XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 8
241000269821 Scombridae Species 0.000 claims description 8
235000020640 mackerel Nutrition 0.000 claims description 8
241000972773 Aulopiformes Species 0.000 claims description 7
235000019419 proteases Nutrition 0.000 claims description 7
235000019515 salmon Nutrition 0.000 claims description 7
229940088594 vitamin Drugs 0.000 claims description 5
229930003231 vitamin Natural products 0.000 claims description 5
235000013343 vitamin Nutrition 0.000 claims description 5
239000011782 vitamin Substances 0.000 claims description 5
241000124008 Mammalia Species 0.000 claims description 4
150000001413 amino acids Chemical class 0.000 claims description 4
239000003795 chemical substances by application Substances 0.000 claims description 4
229910052742 iron Inorganic materials 0.000 claims description 4
150000002632 lipids Chemical class 0.000 claims description 4
241000276438 Gadus morhua Species 0.000 claims description 2
239000013543 active substance Substances 0.000 claims description 2
235000019516 cod Nutrition 0.000 claims description 2
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 2
241001125046 Sardina pilchardus Species 0.000 claims 1
235000018102 proteins Nutrition 0.000 description 33
239000000306 component Substances 0.000 description 20
210000004027 cell Anatomy 0.000 description 17
102000004190 Enzymes Human genes 0.000 description 16
108090000790 Enzymes Proteins 0.000 description 16
229940088598 enzyme Drugs 0.000 description 16
OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 12
CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 8
241001125048 Sardina Species 0.000 description 8
230000000694 effects Effects 0.000 description 8
210000002966 serum Anatomy 0.000 description 8
239000000126 substance Substances 0.000 description 7
NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 6
IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 6
DRTQHJPVMGBUCF-XVFCMESISA-N Uridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-XVFCMESISA-N 0.000 description 6
FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 6
HZAXFHJVJLSVMW-UHFFFAOYSA-N monoethanolamine hydrochloride Natural products NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 6
239000000758 substrate Substances 0.000 description 6
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
239000003921 oil Substances 0.000 description 5
230000004083 survival effect Effects 0.000 description 5
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 4
101150074155 DHFR gene Proteins 0.000 description 4
239000006144 Dulbecco’s modiﬁed Eagle's medium Substances 0.000 description 4
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
229910021578 Iron(III) chloride Inorganic materials 0.000 description 4
DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 4
HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 4
ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 4
AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 4
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 4
CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 4
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
229960005070 ascorbic acid Drugs 0.000 description 4
235000015278 beef Nutrition 0.000 description 4
238000004113 cell culture Methods 0.000 description 4
OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 4
230000012010 growth Effects 0.000 description 4
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 4
RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 4
239000000203 mixture Substances 0.000 description 4
UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 description 3
YKBGVTZYEHREMT-KVQBGUIXSA-N 2'-deoxyguanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](CO)O1 YKBGVTZYEHREMT-KVQBGUIXSA-N 0.000 description 3
DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 3
239000002126 C01EB10 - Adenosine Substances 0.000 description 3
MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 3
UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Natural products O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 description 3
NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 3
108010059378 Endopeptidases Proteins 0.000 description 3
102000005593 Endopeptidases Human genes 0.000 description 3
102000018389 Exopeptidases Human genes 0.000 description 3
108010091443 Exopeptidases Proteins 0.000 description 3
UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 3
102000010781 Interleukin-6 Receptors Human genes 0.000 description 3
108010038501 Interleukin-6 Receptors Proteins 0.000 description 3
KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 3
108090000526 Papain Proteins 0.000 description 3
241000700605 Viruses Species 0.000 description 3
229960005305 adenosine Drugs 0.000 description 3
229940024606 amino acid Drugs 0.000 description 3
235000001014 amino acid Nutrition 0.000 description 3
IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 3
DRTQHJPVMGBUCF-PSQAKQOGSA-N beta-L-uridine Natural products O[C@H]1[C@@H](O)[C@H](CO)O[C@@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-PSQAKQOGSA-N 0.000 description 3
UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 description 3
238000010586 diagram Methods 0.000 description 3
229960002413 ferric citrate Drugs 0.000 description 3
238000001914 filtration Methods 0.000 description 3
229940029575 guanosine Drugs 0.000 description 3
NPFOYSMITVOQOS-UHFFFAOYSA-K iron(III) citrate Chemical compound [Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NPFOYSMITVOQOS-UHFFFAOYSA-K 0.000 description 3
239000000463 material Substances 0.000 description 3
239000002777 nucleoside Substances 0.000 description 3
125000003835 nucleoside group Chemical group 0.000 description 3
230000003204 osmotic effect Effects 0.000 description 3
235000019834 papain Nutrition 0.000 description 3
229940055729 papain Drugs 0.000 description 3
229920001993 poloxamer 188 Polymers 0.000 description 3
238000002360 preparation method Methods 0.000 description 3
230000035755 proliferation Effects 0.000 description 3
239000004094 surface-active agent Substances 0.000 description 3
229940104230 thymidine Drugs 0.000 description 3
229910021654 trace metal Inorganic materials 0.000 description 3
DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 3
229940045145 uridine Drugs 0.000 description 3
UOMQUZPKALKDCA-UHFFFAOYSA-K 2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxymethyl)amino]acetate;iron(3+) Chemical compound [Fe+3].OC(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UOMQUZPKALKDCA-UHFFFAOYSA-K 0.000 description 2
QDGAVODICPCDMU-UHFFFAOYSA-N 2-amino-3-[3-[bis(2-chloroethyl)amino]phenyl]propanoic acid Chemical compound OC(=O)C(N)CC1=CC=CC(N(CCCl)CCCl)=C1 QDGAVODICPCDMU-UHFFFAOYSA-N 0.000 description 2
239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 description 2
PMUNIMVZCACZBB-UHFFFAOYSA-N 2-hydroxyethylazanium;chloride Chemical compound Cl.NCCO PMUNIMVZCACZBB-UHFFFAOYSA-N 0.000 description 2
CKTSBUTUHBMZGZ-CHKWXVPMSA-N 4-amino-1-[(2s,4r,5s)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidin-2-one Chemical compound O=C1N=C(N)C=CN1[C@H]1O[C@@H](CO)[C@H](O)C1 CKTSBUTUHBMZGZ-CHKWXVPMSA-N 0.000 description 2
ALYNCZNDIQEVRV-UHFFFAOYSA-N 4-aminobenzoic acid Chemical compound NC1=CC=C(C(O)=O)C=C1 ALYNCZNDIQEVRV-UHFFFAOYSA-N 0.000 description 2
241000894006 Bacteria Species 0.000 description 2
108091003079 Bovine Serum Albumin Proteins 0.000 description 2
235000019743 Choline chloride Nutrition 0.000 description 2
208000020406 Creutzfeldt Jacob disease Diseases 0.000 description 2
208000003407 Creutzfeldt-Jakob Syndrome Diseases 0.000 description 2
208000010859 Creutzfeldt-Jakob disease Diseases 0.000 description 2
AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 2
CKLJMWTZIZZHCS-UHFFFAOYSA-N D-OH-Asp Natural products OC(=O)C(N)CC(O)=O CKLJMWTZIZZHCS-UHFFFAOYSA-N 0.000 description 2
QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 description 2
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
239000004471 Glycine Substances 0.000 description 2
102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 description 2
108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 description 2
102000004457 Granulocyte-Macrophage Colony-Stimulating Factor Human genes 0.000 description 2
108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 description 2
102000004877 Insulin Human genes 0.000 description 2
108090001061 Insulin Proteins 0.000 description 2
QNAYBMKLOCPYGJ-UWTATZPHSA-N L-Alanine Natural products C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 description 2
CKLJMWTZIZZHCS-UWTATZPHSA-N L-Aspartic acid Natural products OC(=O)[C@H](N)CC(O)=O CKLJMWTZIZZHCS-UWTATZPHSA-N 0.000 description 2
FFEARJCKVFRZRR-UHFFFAOYSA-N L-Methionine Natural products CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 description 2
AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 2
ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 2
QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 description 2
229930064664 L-arginine Natural products 0.000 description 2
235000014852 L-arginine Nutrition 0.000 description 2
239000002211 L-ascorbic acid Substances 0.000 description 2
235000000069 L-ascorbic acid Nutrition 0.000 description 2
CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 description 2
235000019393 L-cystine Nutrition 0.000 description 2
239000004158 L-cystine Substances 0.000 description 2
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 2
229930182816 L-glutamine Natural products 0.000 description 2
AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 2
229930182844 L-isoleucine Natural products 0.000 description 2
239000004395 L-leucine Substances 0.000 description 2
235000019454 L-leucine Nutrition 0.000 description 2
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
229930195722 L-methionine Natural products 0.000 description 2
229930182821 L-proline Natural products 0.000 description 2
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
241000204031 Mycoplasma Species 0.000 description 2
OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 2
PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
241000269908 Platichthys flesus Species 0.000 description 2
RVGRUAULSDPKGF-UHFFFAOYSA-N Poloxamer Chemical compound C1CO1.CC1CO1 RVGRUAULSDPKGF-UHFFFAOYSA-N 0.000 description 2
WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
208000024777 Prion disease Diseases 0.000 description 2
UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
239000004473 Threonine Substances 0.000 description 2
208000018756 Variant Creutzfeldt-Jakob disease Diseases 0.000 description 2
229930003779 Vitamin B12 Natural products 0.000 description 2
DFPAKSUCGFBDDF-ZQBYOMGUSA-N [14c]-nicotinamide Chemical compound N[14C](=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-ZQBYOMGUSA-N 0.000 description 2
229960003767 alanine Drugs 0.000 description 2
235000010323 ascorbic acid Nutrition 0.000 description 2
239000011668 ascorbic acid Substances 0.000 description 2
229960001230 asparagine Drugs 0.000 description 2
229960005261 aspartic acid Drugs 0.000 description 2
229960002685 biotin Drugs 0.000 description 2
235000020958 biotin Nutrition 0.000 description 2
239000011616 biotin Substances 0.000 description 2
208000005881 bovine spongiform encephalopathy Diseases 0.000 description 2
XXWCODXIQWIHQN-UHFFFAOYSA-N butane-1,4-diamine;hydron;dichloride Chemical compound Cl.Cl.NCCCCN XXWCODXIQWIHQN-UHFFFAOYSA-N 0.000 description 2
FAPWYRCQGJNNSJ-UBKPKTQASA-L calcium D-pantothenic acid Chemical compound [Ca+2].OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O.OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O FAPWYRCQGJNNSJ-UBKPKTQASA-L 0.000 description 2
229960002079 calcium pantothenate Drugs 0.000 description 2
229910002091 carbon monoxide Inorganic materials 0.000 description 2
230000010261 cell growth Effects 0.000 description 2
238000005119 centrifugation Methods 0.000 description 2
HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
229960003178 choline chloride Drugs 0.000 description 2
SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 description 2
FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 2
ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 2
229960003067 cystine Drugs 0.000 description 2
230000003247 decreasing effect Effects 0.000 description 2
BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 2
229940031098 ethanolamine Drugs 0.000 description 2
229940073579 ethanolamine hydrochloride Drugs 0.000 description 2
239000012091 fetal bovine serum Substances 0.000 description 2
229960000304 folic acid Drugs 0.000 description 2
235000019152 folic acid Nutrition 0.000 description 2
239000011724 folic acid Substances 0.000 description 2
238000010353 genetic engineering Methods 0.000 description 2
239000008103 glucose Substances 0.000 description 2
229960002743 glutamine Drugs 0.000 description 2
229960002449 glycine Drugs 0.000 description 2
238000010438 heat treatment Methods 0.000 description 2
229960002885 histidine Drugs 0.000 description 2
208000010544 human prion disease Diseases 0.000 description 2
229940125396 insulin Drugs 0.000 description 2
VCJMYUPGQJHHFU-UHFFFAOYSA-N iron(3+);trinitrate Chemical compound [Fe+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O VCJMYUPGQJHHFU-UHFFFAOYSA-N 0.000 description 2
238000002955 isolation Methods 0.000 description 2
229960000310 isoleucine Drugs 0.000 description 2
229960003136 leucine Drugs 0.000 description 2
AGBQKNBQESQNJD-UHFFFAOYSA-M lipoate Chemical compound [O-]C(=O)CCCCC1CCSS1 AGBQKNBQESQNJD-UHFFFAOYSA-M 0.000 description 2
235000019136 lipoic acid Nutrition 0.000 description 2
229910052943 magnesium sulfate Inorganic materials 0.000 description 2
235000019341 magnesium sulphate Nutrition 0.000 description 2
239000003550 marker Substances 0.000 description 2
229960004452 methionine Drugs 0.000 description 2
FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
108090000765 processed proteins & peptides Proteins 0.000 description 2
229960002429 proline Drugs 0.000 description 2
238000000746 purification Methods 0.000 description 2
KIDHWZJUCRJVML-UHFFFAOYSA-N putrescine Chemical compound NCCCCN KIDHWZJUCRJVML-UHFFFAOYSA-N 0.000 description 2
FCHXJFJNDJXENQ-UHFFFAOYSA-N pyridoxal hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(C=O)=C1O FCHXJFJNDJXENQ-UHFFFAOYSA-N 0.000 description 2
RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 2
230000001105 regulatory effect Effects 0.000 description 2
229960002477 riboflavin Drugs 0.000 description 2
235000019192 riboflavin Nutrition 0.000 description 2
239000002151 riboflavin Substances 0.000 description 2
229960001153 serine Drugs 0.000 description 2
IFGCUJZIWBUILZ-UHFFFAOYSA-N sodium 2-[[2-[[hydroxy-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyphosphoryl]amino]-4-methylpentanoyl]amino]-3-(1H-indol-3-yl)propanoic acid Chemical compound [Na+].C=1NC2=CC=CC=C2C=1CC(C(O)=O)NC(=O)C(CC(C)C)NP(O)(=O)OC1OC(C)C(O)C(O)C1O IFGCUJZIWBUILZ-UHFFFAOYSA-N 0.000 description 2
239000011780 sodium chloride Substances 0.000 description 2
235000002639 sodium chloride Nutrition 0.000 description 2
239000011781 sodium selenite Substances 0.000 description 2
235000015921 sodium selenite Nutrition 0.000 description 2
229960001471 sodium selenite Drugs 0.000 description 2
UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
WPLOVIFNBMNBPD-ATHMIXSHSA-N subtilin Chemical compound CC1SCC(NC2=O)C(=O)NC(CC(N)=O)C(=O)NC(C(=O)NC(CCCCN)C(=O)NC(C(C)CC)C(=O)NC(=C)C(=O)NC(CCCCN)C(O)=O)CSC(C)C2NC(=O)C(CC(C)C)NC(=O)C1NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C1NC(=O)C(=C/C)/NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C2NC(=O)CNC(=O)C3CCCN3C(=O)C(NC(=O)C3NC(=O)C(CC(C)C)NC(=O)C(=C)NC(=O)C(CCC(O)=O)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(N)CC=4C5=CC=CC=C5NC=4)CSC3)C(C)SC2)C(C)C)C(C)SC1)CC1=CC=CC=C1 WPLOVIFNBMNBPD-ATHMIXSHSA-N 0.000 description 2
235000000346 sugar Nutrition 0.000 description 2
229960000344 thiamine hydrochloride Drugs 0.000 description 2
DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 2
235000019190 thiamine hydrochloride Nutrition 0.000 description 2
239000011747 thiamine hydrochloride Substances 0.000 description 2
229960002663 thioctic acid Drugs 0.000 description 2
229960002898 threonine Drugs 0.000 description 2
229960004441 tyrosine Drugs 0.000 description 2
235000019163 vitamin B12 Nutrition 0.000 description 2
239000011715 vitamin B12 Substances 0.000 description 2
NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 2
239000011686 zinc sulphate Substances 0.000 description 2
235000009529 zinc sulphate Nutrition 0.000 description 2
OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
PWKSKIMOESPYIA-UHFFFAOYSA-N 2-acetamido-3-sulfanylpropanoic acid Chemical compound CC(=O)NC(CS)C(O)=O PWKSKIMOESPYIA-UHFFFAOYSA-N 0.000 description 1
QWJSAWXRUVVRLH-LREBCSMRSA-M 2-hydroxyethyl(trimethyl)azanium;(2r,3r)-2,3,4-trihydroxy-4-oxobutanoate Chemical compound C[N+](C)(C)CCO.OC(=O)[C@H](O)[C@@H](O)C([O-])=O QWJSAWXRUVVRLH-LREBCSMRSA-M 0.000 description 1
LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 description 1
CKTSBUTUHBMZGZ-ULQXZJNLSA-N 4-amino-1-[(2r,4s,5r)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-tritiopyrimidin-2-one Chemical compound O=C1N=C(N)C([3H])=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 CKTSBUTUHBMZGZ-ULQXZJNLSA-N 0.000 description 1
QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
102000004400 Aminopeptidases Human genes 0.000 description 1
108090000915 Aminopeptidases Proteins 0.000 description 1
241000283690 Bos taurus Species 0.000 description 1
108010004032 Bromelains Proteins 0.000 description 1
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
102000005367 Carboxypeptidases Human genes 0.000 description 1
108010006303 Carboxypeptidases Proteins 0.000 description 1
108090001069 Chymopapain Proteins 0.000 description 1
241000555825 Clupeidae Species 0.000 description 1
WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
102000004860 Dipeptidases Human genes 0.000 description 1
108090001081 Dipeptidases Proteins 0.000 description 1
102000003951 Erythropoietin Human genes 0.000 description 1
108090000394 Erythropoietin Proteins 0.000 description 1
102000001690 Factor VIII Human genes 0.000 description 1
108010054218 Factor VIII Proteins 0.000 description 1
108090000270 Ficain Proteins 0.000 description 1
108010028690 Fish Proteins Proteins 0.000 description 1
229930091371 Fructose Natural products 0.000 description 1
239000005715 Fructose Substances 0.000 description 1
RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
229930182566 Gentamicin Natural products 0.000 description 1
CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
239000007995 HEPES buffer Substances 0.000 description 1
102000004157 Hydrolases Human genes 0.000 description 1
108090000604 Hydrolases Proteins 0.000 description 1
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
108010050904 Interferons Proteins 0.000 description 1
102000014150 Interferons Human genes 0.000 description 1
108010002352 Interleukin-1 Proteins 0.000 description 1
102000004889 Interleukin-6 Human genes 0.000 description 1
108090001005 Interleukin-6 Proteins 0.000 description 1
108010063738 Interleukins Proteins 0.000 description 1
102000015696 Interleukins Human genes 0.000 description 1
150000008575 L-amino acids Chemical class 0.000 description 1
150000008539 L-glutamic acids Chemical class 0.000 description 1
241000269795 Lateolabrax japonicus Species 0.000 description 1
239000007993 MOPS buffer Substances 0.000 description 1
229910021586 Nickel(II) chloride Inorganic materials 0.000 description 1
239000005642 Oleic acid Substances 0.000 description 1
ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 description 1
239000004105 Penicillin G potassium Substances 0.000 description 1
102000002508 Peptide Elongation Factors Human genes 0.000 description 1
108010068204 Peptide Elongation Factors Proteins 0.000 description 1
BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 1
102000029797 Prion Human genes 0.000 description 1
108091000054 Prion Proteins 0.000 description 1
239000005700 Putrescine Substances 0.000 description 1
108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
240000004808 Saccharomyces cerevisiae Species 0.000 description 1
108010071390 Serum Albumin Proteins 0.000 description 1
102000007562 Serum Albumin Human genes 0.000 description 1
UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
241000656145 Thyrsites atun Species 0.000 description 1
ISWQCIVKKSOKNN-UHFFFAOYSA-L Tiron Chemical compound [Na+].[Na+].OC1=CC(S([O-])(=O)=O)=CC(S([O-])(=O)=O)=C1O ISWQCIVKKSOKNN-UHFFFAOYSA-L 0.000 description 1
108050006955 Tissue-type plasminogen activator Proteins 0.000 description 1
102100033571 Tissue-type plasminogen activator Human genes 0.000 description 1
241001504592 Trachurus trachurus Species 0.000 description 1
102000004338 Transferrin Human genes 0.000 description 1
108090000901 Transferrin Proteins 0.000 description 1
108090000435 Urokinase-type plasminogen activator Proteins 0.000 description 1
102000003990 Urokinase-type plasminogen activator Human genes 0.000 description 1
230000002159 abnormal effect Effects 0.000 description 1
238000001042 affinity chromatography Methods 0.000 description 1
SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 description 1
WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
229960004050 aminobenzoic acid Drugs 0.000 description 1
239000003242 anti bacterial agent Substances 0.000 description 1
229940088710 antibiotic agent Drugs 0.000 description 1
239000012298 atmosphere Substances 0.000 description 1
239000007640 basal medium Substances 0.000 description 1
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
238000009835 boiling Methods 0.000 description 1
235000019835 bromelain Nutrition 0.000 description 1
239000001110 calcium chloride Substances 0.000 description 1
229910001628 calcium chloride Inorganic materials 0.000 description 1
239000006143 cell culture medium Substances 0.000 description 1
235000012000 cholesterol Nutrition 0.000 description 1
229960002976 chymopapain Drugs 0.000 description 1
238000003776 cleavage reaction Methods 0.000 description 1
238000011109 contamination Methods 0.000 description 1
238000005520 cutting process Methods 0.000 description 1
238000004925 denaturation Methods 0.000 description 1
230000036425 denaturation Effects 0.000 description 1
201000010099 disease Diseases 0.000 description 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
229940105423 erythropoietin Drugs 0.000 description 1
235000020774 essential nutrients Nutrition 0.000 description 1
HQPMKSGTIOYHJT-UHFFFAOYSA-N ethane-1,2-diol;propane-1,2-diol Chemical compound OCCO.CC(O)CO HQPMKSGTIOYHJT-UHFFFAOYSA-N 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
239000011790 ferrous sulphate Substances 0.000 description 1
235000003891 ferrous sulphate Nutrition 0.000 description 1
102000013361 fetuin Human genes 0.000 description 1
108060002885 fetuin Proteins 0.000 description 1
235000019836 ficin Nutrition 0.000 description 1
POTUGHMKJGOKRI-UHFFFAOYSA-N ficin Chemical compound FI=CI=N POTUGHMKJGOKRI-UHFFFAOYSA-N 0.000 description 1
229930182830 galactose Natural products 0.000 description 1
229960002518 gentamicin Drugs 0.000 description 1
229960002989 glutamic acid Drugs 0.000 description 1
238000000227 grinding Methods 0.000 description 1
238000004128 high performance liquid chromatography Methods 0.000 description 1
239000012510 hollow fiber Substances 0.000 description 1
210000004408 hybridoma Anatomy 0.000 description 1
239000000413 hydrolysate Substances 0.000 description 1
230000016784 immunoglobulin production Effects 0.000 description 1
CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
229940079322 interferon Drugs 0.000 description 1
229940047122 interleukins Drugs 0.000 description 1
BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 description 1
229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
229910000360 iron(III) sulfate Inorganic materials 0.000 description 1
QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
230000007774 longterm Effects 0.000 description 1
229910001629 magnesium chloride Inorganic materials 0.000 description 1
210000004962 mammalian cell Anatomy 0.000 description 1
239000011702 manganese sulphate Substances 0.000 description 1
235000007079 manganese sulphate Nutrition 0.000 description 1
SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
244000005700 microbiome Species 0.000 description 1
QMMRZOWCJAIUJA-UHFFFAOYSA-L nickel dichloride Chemical compound Cl[Ni]Cl QMMRZOWCJAIUJA-UHFFFAOYSA-L 0.000 description 1
235000001968 nicotinic acid Nutrition 0.000 description 1
229960003512 nicotinic acid Drugs 0.000 description 1
239000011664 nicotinic acid Substances 0.000 description 1
ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
235000021313 oleic acid Nutrition 0.000 description 1
229960003104 ornithine Drugs 0.000 description 1
239000006174 pH buffer Substances 0.000 description 1
230000001717 pathogenic effect Effects 0.000 description 1
235000019368 penicillin G potassium Nutrition 0.000 description 1
229940056360 penicillin g Drugs 0.000 description 1
239000007793 ph indicator Substances 0.000 description 1
229960003531 phenolsulfonphthalein Drugs 0.000 description 1
235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
229920000053 polysorbate 80 Polymers 0.000 description 1
239000001103 potassium chloride Substances 0.000 description 1
235000011164 potassium chloride Nutrition 0.000 description 1
239000004323 potassium nitrate Substances 0.000 description 1
235000010333 potassium nitrate Nutrition 0.000 description 1
OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 description 1
ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 description 1
229960004172 pyridoxine hydrochloride Drugs 0.000 description 1
235000019171 pyridoxine hydrochloride Nutrition 0.000 description 1
239000011764 pyridoxine hydrochloride Substances 0.000 description 1
235000020989 red meat Nutrition 0.000 description 1
229930002330 retinoic acid Natural products 0.000 description 1
230000007017 scission Effects 0.000 description 1
CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
239000011734 sodium Substances 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
235000017557 sodium bicarbonate Nutrition 0.000 description 1
229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
239000007787 solid Substances 0.000 description 1
229960005322 streptomycin Drugs 0.000 description 1
150000008163 sugars Chemical class 0.000 description 1
238000010998 test method Methods 0.000 description 1
HPGGPRDJHPYFRM-UHFFFAOYSA-J tin(iv) chloride Chemical compound Cl[Sn](Cl)(Cl)Cl HPGGPRDJHPYFRM-UHFFFAOYSA-J 0.000 description 1
239000012581 transferrin Substances 0.000 description 1
230000014616 translation Effects 0.000 description 1
229960001727 tretinoin Drugs 0.000 description 1
229960005356 urokinase Drugs 0.000 description 1
229960004295 valine Drugs 0.000 description 1
235000020990 white meat Nutrition 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/40—Nucleotides, nucleosides or bases
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/70—Undefined extracts
- C12N2500/80—Undefined extracts from animals

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Chemical & Material Sciences (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
Zoology (AREA)
Biomedical Technology (AREA)
Genetics & Genomics (AREA)
Bioinformatics & Cheminformatics (AREA)
General Health & Medical Sciences (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
Cell Biology (AREA)
Molecular Biology (AREA)
General Chemical & Material Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Peptides Or Proteins (AREA)

NO20006096A 1998-06-01 2000-11-30 Medium for dyrking av dyreceller og fremgangsmate for fremstilling av protein ved anvendelse av de samme NO326180B1 (no)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
JP15095798		1998-06-01
PCT/JP1999/002904 WO1999063058A1 (en)	1998-06-01	1999-06-01	Media for culturing animal cells and process for producing protein by using the same

Publications (3)

Publication Number	Publication Date
NO20006096D0 NO20006096D0 (no)	2000-11-30
NO20006096L NO20006096L (no)	2001-02-01
NO326180B1 true NO326180B1 (no)	2008-10-13

Family

ID=15508136

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
NO20006096A NO326180B1 (no)	1998-06-01	2000-11-30	Medium for dyrking av dyreceller og fremgangsmate for fremstilling av protein ved anvendelse av de samme
NO2009012C NO2009012I1 (no)	1998-06-01	2009-05-28	Humanisert anti-humant IL-6 reseptor monoklonalt antistoff

Family Applications After (1)

Application Number	Title	Priority Date	Filing Date
NO2009012C NO2009012I1 (no)	1998-06-01	2009-05-28	Humanisert anti-humant IL-6 reseptor monoklonalt antistoff

Country Status (15)

Country	Link
US (2)	US6537782B1 (da)
EP (1)	EP1085083B1 (da)
JP (1)	JP3510858B2 (da)
KR (1)	KR100430447B1 (da)
CN (1)	CN1187441C (da)
AT (1)	ATE361360T1 (da)
AU (1)	AU744715B2 (da)
CA (1)	CA2333966C (da)
DE (1)	DE69935978T2 (da)
DK (1)	DK1085083T3 (da)
ES (1)	ES2285840T3 (da)
IL (1)	IL139974A (da)
NO (2)	NO326180B1 (da)
RU (2)	RU2214455C2 (da)
WO (1)	WO1999063058A1 (da)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
AU736282B2 (en)	1997-03-21	2001-07-26	Chugai Seiyaku Kabushiki Kaisha	A preventive or therapeutic agent for sensitized T cell- mediated diseases comprising IL-6 antagonist as an active ingredient
ATE264385T1 (de) *	1999-12-28	2004-04-15	Iso Tis N V	Wachstumsfaktoren und l-glutamin enthaltendes zellkulturmedium
UA80091C2 (en)	2001-04-02	2007-08-27	Chugai Pharmaceutical Co Ltd	Remedies for infant chronic arthritis-relating diseases and still's disease which contain an interleukin-6 (il-6) antagonist
DE10124820A1 (de) *	2001-05-21	2002-12-05	Heinrich Wieland	Zusammensetzung und Verwendung von Substanzen zur Stabilisierung von schwefelhaltigen Aminosäuren im Blut
AU2003211990A1 (en) *	2002-02-14	2003-09-04	Chugai Seiyaku Kabushiki Kaisha	Antibody-containing solution pharmaceuticals
GB2401040A (en) *	2003-04-28	2004-11-03	Chugai Pharmaceutical Co Ltd	Method for treating interleukin-6 related diseases
US8398980B2 (en)	2004-03-24	2013-03-19	Chugai Seiyaku Kabushiki Kaisha	Subtypes of humanized antibody against interleuken-6 receptor
TWI374935B (en) *	2004-08-27	2012-10-21	Pfizer Ireland Pharmaceuticals	Production of α-abeta
US20060094104A1 (en) *	2004-10-29	2006-05-04	Leopold Grillberger	Animal protein-free media for cultivation of cells
KR100679112B1 (ko) *	2004-11-17	2007-02-07	삼성정밀화학 주식회사	동물세포 배양방법
JP4818936B2 (ja) *	2005-01-05	2011-11-16	中外製薬株式会社	細胞の培養方法およびその利用
EP3026109B1 (en) *	2005-12-08	2022-03-09	Amgen Inc.	Improved production of glycoproteins using manganese
EP3653699A1 (en)	2006-01-04	2020-05-20	Baxalta Incorporated	Oligopeptide-free cell culture media
PE20091174A1 (es)	2007-12-27	2009-08-03	Chugai Pharmaceutical Co Ltd	Formulacion liquida con contenido de alta concentracion de anticuerpo
WO2011051231A1 (en)	2009-10-26	2011-05-05	F. Hoffmann-La Roche Ag	Method for the production of a glycosylated immunoglobulin
MD191Z5 (ro) *	2010-01-21	2010-11-30	Государственный Медицинский И Фармацевтический Университет "Nicolae Testemitanu" Республики Молдова	Mediu nutritiv pentru cultivarea celulelor de origine animală
TWI603738B (zh)	2010-11-08	2017-11-01	建南德克公司	皮下投予抗－ｉｌ－６受體抗體
CN105378480B (zh)	2013-07-04	2018-06-12	豪夫迈·罗氏有限公司	检测血清样品中抗药物抗体的干扰抑制性免疫测定
ES2782380T5 (es)	2013-10-30	2023-05-08	Univ Missouri	Método de especificación y cultivo de linaje del músculo esquelético escalable
KR20180009760A (ko)	2015-05-27	2018-01-29	아스테라스 세이야쿠 가부시키가이샤	핵산 함유 배지를 이용한 세포 배양 방법
US11484591B2 (en)	2016-02-22	2022-11-01	Ohio State Innovation Foundation	Chemoprevention using controlled-release formulations of anti-interleukin 6 agents, synthetic vitamin A analogues or metabolites, and estradiol metabolites
WO2018170405A1 (en)	2017-03-17	2018-09-20	Ohio State Innovation Foundation	Nanoparticles for delivery of chemopreventive agents
US11976302B2 (en)	2017-05-06	2024-05-07	Upside Foods, Inc.	Compositions and methods for increasing the culture density of a cellular biomass within a cultivation infrastructure
US11479792B2 (en)	2017-07-13	2022-10-25	Upside Foods, Inc.	Compositions and methods for increasing the efficiency of cell cultures used for food production
JP2022527972A (ja)	2019-04-02	2022-06-07	アンスティチュナショナルドゥラサンテエドゥラルシェルシュメディカル	前悪性病変を有する患者において癌を予測及び予防する方法
JP7166039B1 (ja)	2020-12-22	2022-11-07	株式会社マルハチ村松	ペプチド、細胞増殖促進剤、タンパク質産生促進剤、培地、該ペプチドを用いた細胞増殖方法、及び、該ペプチドを用いたタンパク質産生方法
WO2023240152A1 (en)	2022-06-07	2023-12-14	Upside Foods, Inc.	Engineering cell lines capable of proliferation in growth factor free media formulations

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS509877B2 (da) *	1972-09-13	1975-04-16
US3962425A (en) *	1972-09-21	1976-06-08	Weyerhaeuser Company	Ruminant repellent from enzymatically putrefied lipoidal material
JPS5336033B2 (da) *	1972-12-11	1978-09-30
JPH01148181A (ja) *	1987-12-07	1989-06-09	Jgc Corp	グルタチオンの菌体内蓄積法
JPH0239882A (ja) *	1988-07-29	1990-02-08	Japan Synthetic Rubber Co Ltd	動物細胞培養用培地
JPH0638704A (ja) *	1991-06-19	1994-02-15	Morinaga Milk Ind Co Ltd	発酵調味料及びその製造法
US5426045A (en) *	1993-12-16	1995-06-20	Sea Run Holdings, Inc.	Method for culturing mammalian cells in a medium containing fish serum
WO1995023212A1 (en)	1994-02-23	1995-08-31	Sea Run Holdings, Inc.	Method for culturing mammalian cells and insect cells in a medium containing fish serum
RU2103360C1 (ru) *	1996-07-25	1998-01-27	Инна Георгиевна Ермишина	Питательная среда для культивирования клеток эукариотов и способ получения основы питательной среды - протеолитического гидролизата
CN1157694A (zh)	1996-10-15	1997-08-27	玉环县海天营养源有限公司	一种液化鱼蛋白粉的制作方法

1999
- 1999-01-01 US US09/701,518 patent/US6537782B1/en not_active Expired - Lifetime
- 1999-06-01 DE DE69935978T patent/DE69935978T2/de not_active Expired - Lifetime
- 1999-06-01 DK DK99922611T patent/DK1085083T3/da active
- 1999-06-01 AT AT99922611T patent/ATE361360T1/de active
- 1999-06-01 RU RU2000133243/13A patent/RU2214455C2/ru active
- 1999-06-01 IL IL13997499A patent/IL139974A/xx not_active IP Right Cessation
- 1999-06-01 WO PCT/JP1999/002904 patent/WO1999063058A1/ja not_active Ceased
- 1999-06-01 CA CA002333966A patent/CA2333966C/en not_active Expired - Lifetime
- 1999-06-01 EP EP99922611A patent/EP1085083B1/en not_active Expired - Lifetime
- 1999-06-01 CN CNB998085766A patent/CN1187441C/zh not_active Expired - Lifetime
- 1999-06-01 RU RU2003113779/13A patent/RU2333242C2/ru active
- 1999-06-01 ES ES99922611T patent/ES2285840T3/es not_active Expired - Lifetime
- 1999-06-01 AU AU39584/99A patent/AU744715B2/en not_active Expired
- 1999-06-01 JP JP2000552254A patent/JP3510858B2/ja not_active Expired - Lifetime
- 1999-06-01 KR KR10-2000-7013548A patent/KR100430447B1/ko not_active Expired - Lifetime
2000
- 2000-11-30 NO NO20006096A patent/NO326180B1/no not_active IP Right Cessation
2003
- 2003-01-06 US US10/336,825 patent/US6962812B2/en not_active Expired - Lifetime
2009
- 2009-05-28 NO NO2009012C patent/NO2009012I1/no not_active IP Right Cessation

Also Published As

Publication number	Publication date
NO20006096L (no)	2001-02-01
HK1037678A1 (en)	2002-02-15
IL139974A (en)	2005-09-25
JP3510858B2 (ja)	2004-03-29
IL139974A0 (en)	2002-02-10
AU744715B2 (en)	2002-02-28
ES2285840T3 (es)	2007-11-16
KR20010071359A (ko)	2001-07-28
WO1999063058A1 (en)	1999-12-09
AU744715C (en)	1999-12-20
DE69935978T2 (de)	2008-01-17
US6537782B1 (en)	2003-03-25
DE69935978D1 (de)	2007-06-14
EP1085083B1 (en)	2007-05-02
NO2009012I1 (no)	2009-06-15
ATE361360T1 (de)	2007-05-15
CN1309698A (zh)	2001-08-22
RU2214455C2 (ru)	2003-10-20
US6962812B2 (en)	2005-11-08
CA2333966C (en)	2006-07-25
RU2333242C2 (ru)	2008-09-10
KR100430447B1 (ko)	2004-05-10
CN1187441C (zh)	2005-02-02
CA2333966A1 (en)	1999-12-09
DK1085083T3 (da)	2007-06-18
EP1085083A4 (en)	2002-11-27
NO20006096D0 (no)	2000-11-30
AU3958499A (en)	1999-12-20
US20030096372A1 (en)	2003-05-22
EP1085083A1 (en)	2001-03-21

Legal Events

Date

Code

Title

Description

2014-07-14

SPCW

Withdrawal, rejection or dismissal of supplementary protection certificate

Free format text: PRODUCT NAME: ROACTEMRA-TOCILIZUMAB; NAT. REGISTRATION NO/DATE: EU/1/08/492/001/NO-006/NO 20090206; FIRST REGISTRATION: EU EU/1/08/492/001-006 20081202

Spc suppl protection certif: 2009012

Filing date: 20090528

2019-06-11

MK1K

Patent expired

Publication	Publication Date	Title
NO326180B1 (no)	2008-10-13	Medium for dyrking av dyreceller og fremgangsmate for fremstilling av protein ved anvendelse av de samme
KR101362805B1 (ko)	2014-02-14	개선된 세포 배양 배지
JPWO1999063058A1 (ja)	2002-11-19	動物細胞培養用培地およびそれを使用してタンパク質を製造する方法
HK1207883A1 (en)	2016-02-12	Cell culture method and utilization of the same
JP3822137B2 (ja)	2006-09-13	動物細胞培養用培地の添加剤およびそれを用いたタンパク質の製造方法
CA3206235A1 (en)	2022-08-18	Nutrient media for cell culture containing plant protein hydrolysates
JP3950834B2 (ja)	2007-08-01	動物細胞培養用培地およびそれを使用してタンパク質を製造する方法
HK1034748A (en)	2001-11-02	Media for culturing animal cells and process for producing protein by using the same
HK1037678B (en)	2005-04-22	Media for culturing animal cells and process for producing protein by using the same