NO774312L - Ne ny cellelinje, samt fremgangsmaate for viderefoering av den - Google Patents
Ne ny cellelinje, samt fremgangsmaate for viderefoering av denInfo
- Publication number
- NO774312L NO774312L NO774312A NO774312A NO774312L NO 774312 L NO774312 L NO 774312L NO 774312 A NO774312 A NO 774312A NO 774312 A NO774312 A NO 774312A NO 774312 L NO774312 L NO 774312L
- Authority
- NO
- Norway
- Prior art keywords
- cells
- culture
- cell
- medium
- cell line
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 15
- 210000004027 cell Anatomy 0.000 claims description 79
- 239000002609 medium Substances 0.000 claims description 16
- 235000015097 nutrients Nutrition 0.000 claims description 12
- 238000004113 cell culture Methods 0.000 claims description 9
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 7
- 239000012894 fetal calf serum Substances 0.000 claims description 7
- 238000004114 suspension culture Methods 0.000 claims description 7
- 239000012980 RPMI-1640 medium Substances 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 4
- 230000003068 static effect Effects 0.000 claims description 4
- 230000000644 propagated effect Effects 0.000 claims description 3
- 238000009987 spinning Methods 0.000 claims description 3
- 229920001817 Agar Polymers 0.000 claims description 2
- 210000000712 G cell Anatomy 0.000 claims description 2
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 claims description 2
- 239000008272 agar Substances 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 claims 1
- 239000008188 pellet Substances 0.000 claims 1
- 230000001902 propagating effect Effects 0.000 claims 1
- 239000000427 antigen Substances 0.000 description 8
- 102000036639 antigens Human genes 0.000 description 8
- 108091007433 antigens Proteins 0.000 description 8
- 210000000265 leukocyte Anatomy 0.000 description 8
- 150000007523 nucleic acids Chemical class 0.000 description 8
- 102000039446 nucleic acids Human genes 0.000 description 8
- 108020004707 nucleic acids Proteins 0.000 description 8
- 241000282414 Homo sapiens Species 0.000 description 6
- 239000000385 dialysis solution Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 241000700605 Viruses Species 0.000 description 3
- 230000001900 immune effect Effects 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 210000004698 lymphocyte Anatomy 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 230000000890 antigenic effect Effects 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 210000002865 immune cell Anatomy 0.000 description 2
- 210000005259 peripheral blood Anatomy 0.000 description 2
- 239000011886 peripheral blood Substances 0.000 description 2
- 230000009257 reactivity Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- FUFLCEKSBBHCMO-UHFFFAOYSA-N 11-dehydrocorticosterone Natural products O=C1CCC2(C)C3C(=O)CC(C)(C(CC4)C(=O)CO)C4C3CCC2=C1 FUFLCEKSBBHCMO-UHFFFAOYSA-N 0.000 description 1
- 102000007644 Colony-Stimulating Factors Human genes 0.000 description 1
- 108010071942 Colony-Stimulating Factors Proteins 0.000 description 1
- MFYSYFVPBJMHGN-ZPOLXVRWSA-N Cortisone Chemical compound O=C1CC[C@]2(C)[C@H]3C(=O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 MFYSYFVPBJMHGN-ZPOLXVRWSA-N 0.000 description 1
- MFYSYFVPBJMHGN-UHFFFAOYSA-N Cortisone Natural products O=C1CCC2(C)C3C(=O)CC(C)(C(CC4)(O)C(=O)CO)C4C3CCC2=C1 MFYSYFVPBJMHGN-UHFFFAOYSA-N 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010034719 Personality change Diseases 0.000 description 1
- 239000003470 adrenal cortex hormone Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000721 bacterilogical effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 229960004544 cortisone Drugs 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 238000000635 electron micrograph Methods 0.000 description 1
- 210000002472 endoplasmic reticulum Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 210000005210 lymphoid organ Anatomy 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000000242 pagocytic effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 108010088880 plasmagel Proteins 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0635—B lymphocytes
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Data Exchanges In Wide-Area Networks (AREA)
- Vehicle Body Suspensions (AREA)
- Electrochromic Elements, Electrophoresis, Or Variable Reflection Or Absorption Elements (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB52675/76A GB1592954A (en) | 1976-12-16 | 1976-12-16 | Cell line |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NO774312L true NO774312L (no) | 1978-06-19 |
Family
ID=10464838
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO774312A NO774312L (no) | 1976-12-16 | 1977-12-14 | Ne ny cellelinje, samt fremgangsmaate for viderefoering av den |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US4316962A (da) |
| JP (1) | JPS5386077A (da) |
| AT (1) | AT353408B (da) |
| BE (1) | BE861858A (da) |
| CA (1) | CA1117044A (da) |
| DK (1) | DK557577A (da) |
| ES (1) | ES465060A1 (da) |
| FI (1) | FI773785A7 (da) |
| FR (1) | FR2374418A1 (da) |
| GB (1) | GB1592954A (da) |
| LU (1) | LU78689A1 (da) |
| NL (1) | NL7713834A (da) |
| NO (1) | NO774312L (da) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8614733D0 (en) * | 1986-06-17 | 1986-07-23 | Lefesvre A | Aids treatment |
| AU7317400A (en) * | 1999-09-21 | 2001-04-24 | Chugai Research Institute For Molecular Medicine, Inc. | Transporter genes oatp-b, c, d and e |
-
1976
- 1976-12-16 GB GB52675/76A patent/GB1592954A/en not_active Expired
-
1977
- 1977-12-14 DK DK557577A patent/DK557577A/da unknown
- 1977-12-14 FI FI773785A patent/FI773785A7/fi not_active Application Discontinuation
- 1977-12-14 ES ES465060A patent/ES465060A1/es not_active Expired
- 1977-12-14 US US05/860,439 patent/US4316962A/en not_active Expired - Lifetime
- 1977-12-14 NL NL7713834A patent/NL7713834A/xx not_active Application Discontinuation
- 1977-12-14 JP JP15040777A patent/JPS5386077A/ja active Pending
- 1977-12-14 FR FR7737743A patent/FR2374418A1/fr active Granted
- 1977-12-14 CA CA000293060A patent/CA1117044A/en not_active Expired
- 1977-12-14 NO NO774312A patent/NO774312L/no unknown
- 1977-12-14 LU LU78689A patent/LU78689A1/xx unknown
- 1977-12-14 BE BE183461A patent/BE861858A/xx unknown
- 1977-12-14 AT AT895077A patent/AT353408B/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| ATA895077A (de) | 1979-04-15 |
| JPS5386077A (en) | 1978-07-29 |
| GB1592954A (en) | 1981-07-15 |
| NL7713834A (nl) | 1978-06-20 |
| US4316962A (en) | 1982-02-23 |
| FI773785A7 (fi) | 1978-06-17 |
| FR2374418A1 (fr) | 1978-07-13 |
| DK557577A (da) | 1978-06-17 |
| LU78689A1 (fr) | 1979-06-13 |
| ES465060A1 (es) | 1978-09-01 |
| BE861858A (fr) | 1978-06-14 |
| CA1117044A (en) | 1982-01-26 |
| FR2374418B3 (da) | 1981-02-06 |
| AT353408B (de) | 1979-11-12 |
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