NO843838L - Fremgangsmaate for detektering av nukleinsyre - Google Patents
Fremgangsmaate for detektering av nukleinsyreInfo
- Publication number
- NO843838L NO843838L NO843838A NO843838A NO843838L NO 843838 L NO843838 L NO 843838L NO 843838 A NO843838 A NO 843838A NO 843838 A NO843838 A NO 843838A NO 843838 L NO843838 L NO 843838L
- Authority
- NO
- Norway
- Prior art keywords
- nucleic acid
- gene
- detection
- probe
- base sequence
- Prior art date
Links
- 108020004707 nucleic acids Proteins 0.000 title claims description 62
- 102000039446 nucleic acids Human genes 0.000 title claims description 62
- 150000007523 nucleic acids Chemical class 0.000 title claims description 62
- 238000000034 method Methods 0.000 title claims description 40
- 239000011859 microparticle Substances 0.000 claims description 28
- 108020004711 Nucleic Acid Probes Proteins 0.000 claims description 23
- 238000001514 detection method Methods 0.000 claims description 23
- 239000002853 nucleic acid probe Substances 0.000 claims description 23
- 108090000623 proteins and genes Proteins 0.000 claims description 23
- 239000000523 sample Substances 0.000 claims description 23
- 108090001008 Avidin Proteins 0.000 claims description 14
- 239000007790 solid phase Substances 0.000 claims description 14
- 238000002372 labelling Methods 0.000 claims description 12
- 108020004414 DNA Proteins 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 8
- 238000009396 hybridization Methods 0.000 claims description 7
- 102000053602 DNA Human genes 0.000 claims description 6
- 239000000020 Nitrocellulose Substances 0.000 claims description 6
- 229920001220 nitrocellulos Polymers 0.000 claims description 6
- 230000000155 isotopic effect Effects 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 2
- 238000000149 argon plasma sintering Methods 0.000 claims 2
- 238000003776 cleavage reaction Methods 0.000 claims 2
- 238000004737 colorimetric analysis Methods 0.000 claims 2
- 230000023077 detection of light stimulus Effects 0.000 claims 2
- 230000002255 enzymatic effect Effects 0.000 claims 2
- 230000007017 scission Effects 0.000 claims 2
- 239000002253 acid Substances 0.000 claims 1
- 239000011230 binding agent Substances 0.000 claims 1
- 230000027455 binding Effects 0.000 description 7
- 239000000463 material Substances 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 229960002685 biotin Drugs 0.000 description 5
- 239000011616 biotin Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 230000008878 coupling Effects 0.000 description 3
- 238000010168 coupling process Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 241000276498 Pollachius virens Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 231100000206 health hazard Toxicity 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- NMHMNPHRMNGLLB-UHFFFAOYSA-N phloretic acid Chemical compound OC(=O)CCC1=CC=C(O)C=C1 NMHMNPHRMNGLLB-UHFFFAOYSA-N 0.000 description 1
- 229920000193 polymethacrylate Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US53613883A | 1983-09-26 | 1983-09-26 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NO843838L true NO843838L (no) | 1985-03-27 |
Family
ID=24137316
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO843838A NO843838L (no) | 1983-09-26 | 1984-09-25 | Fremgangsmaate for detektering av nukleinsyre |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0139489A3 (fr) |
| JP (1) | JPS6093355A (fr) |
| AU (1) | AU3355484A (fr) |
| CA (1) | CA1256005A (fr) |
| NO (1) | NO843838L (fr) |
Families Citing this family (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3586689T2 (de) * | 1984-02-28 | 1993-04-08 | Ortho Diagnostic Systems Inc | Diagnose von genmissbildungen durch restriktionskarte-analysierung unter anwendung eines sandwichhybridisierungsformats. |
| US5288609A (en) * | 1984-04-27 | 1994-02-22 | Enzo Diagnostics, Inc. | Capture sandwich hybridization method and composition |
| CA1260372A (fr) * | 1984-04-27 | 1989-09-26 | Elazar Rabbani | Methode d'hybridation pour la detection de materiaux genetiques |
| US4766062A (en) * | 1984-05-07 | 1988-08-23 | Allied Corporation | Displacement polynucleotide assay method and polynucleotide complex reagent therefor |
| US4766064A (en) * | 1984-05-07 | 1988-08-23 | Allied Corporation | Displacement polynucleotide assay employing polyether and diagnostic kit |
| CA1253777A (fr) * | 1984-06-01 | 1989-05-09 | Robert J. Carrico | Essai d'hybridation de l'acide nucleique au moyen de sondes arn immobilisees |
| EP0184701B1 (fr) * | 1984-12-03 | 1994-06-08 | Hoechst Celanese Corporation | Procédé pour déterminer un ligand |
| FI72146C (fi) * | 1985-01-02 | 1987-04-13 | Orion Yhtymae Oy | Foerfarande foer identifiering av nukleinsyror. |
| CA1272443A (fr) * | 1985-02-22 | 1990-08-07 | Nanibhushan Dattagupta | Essai de double hybridation en solution-phase pour deceler des sequences polynucleotidiques |
| WO1986005519A1 (fr) * | 1985-03-15 | 1986-09-25 | James Summerton | Reactif et procede d'analyse de polynucleotides |
| GB8509367D0 (en) * | 1985-04-12 | 1985-05-15 | Amersham Int Plc | Nucleic acid hybridisation |
| ES8707343A1 (es) * | 1985-06-13 | 1987-07-16 | Amgen | Un metodo para aislar una secuencia de acido nucleico objetivo seleccionada |
| US5273882A (en) * | 1985-06-13 | 1993-12-28 | Amgen | Method and kit for performing nucleic acid hybridization assays |
| US4868104A (en) * | 1985-09-06 | 1989-09-19 | Syntex (U.S.A.) Inc. | Homogeneous assay for specific polynucleotides |
| US4868105A (en) * | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
| US4882269A (en) * | 1985-12-13 | 1989-11-21 | Princeton University | Amplified hybridization assay |
| AU6834187A (en) * | 1985-12-17 | 1987-07-15 | Genetics Institute Inc. | Displacement polynucleotide method and reagent complex |
| US4818680A (en) * | 1985-12-18 | 1989-04-04 | Mary Collins | Method and kit involving displacement and rehybridization of labeled polynucleotide |
| US4925785A (en) * | 1986-03-07 | 1990-05-15 | Biotechnica Diagnostics, Inc. | Nucleic acid hybridization assays |
| FR2601455B1 (fr) * | 1986-07-11 | 1989-08-04 | Stallergenes Lab | Nouveaux reactifs biologiques en phase solide et leur procede d'obtention |
| AU8103687A (en) * | 1986-10-14 | 1988-05-06 | Beckman Instruments, Inc. | Improved nucleic acid hybridization technique and kit therefor |
| US5750338A (en) * | 1986-10-23 | 1998-05-12 | Amoco Corporation | Target and background capture methods with amplification for affinity assays |
| US5714380A (en) * | 1986-10-23 | 1998-02-03 | Amoco Corporation | Closed vessel for isolating target molecules and for performing amplification |
| ZA877772B (en) * | 1986-10-23 | 1988-04-20 | Amoco Corporation | Target and background capture methods and apparatus for affinity assays |
| US5599667A (en) * | 1987-03-02 | 1997-02-04 | Gen-Probe Incorporated | Polycationic supports and nucleic acid purification separation and hybridization |
| AU601021B2 (en) * | 1987-03-11 | 1990-08-30 | Molecular Diagnostics, Inc. | Assay for necleic acid sequences in a sample |
| AU622104B2 (en) * | 1987-03-11 | 1992-04-02 | Sangtec Molecular Diagnostics Ab | Method of assaying of nucleic acids, a reagent combination and kit therefore |
| JPH02503983A (ja) * | 1987-06-26 | 1990-11-22 | イー・アイ・デュポン・ドゥ・ヌムール・アンド・カンパニー | 捕捉ビーズを用いる組み換えクローン化naからの混入配列のアフイニテイ除去 |
| EP0305145A3 (fr) * | 1987-08-24 | 1990-05-02 | Ortho Diagnostic Systems Inc. | Méthodes et sondes pour détecter des acides nucléiques |
| FI80476C (fi) * | 1987-10-09 | 1990-06-11 | Orion Yhtymae Oy | Foerbaettrat hybridisationsfoerfarande, vid foerfarandet anvaent redskap och reagensfoerpackning. |
| CA1312277C (fr) * | 1987-12-18 | 1993-01-05 | Richard C. Sutton | Reactifs immobilises par l'avidine et la biotine, elements d'analyse et methodes d'utilisation |
| JPH03504199A (ja) * | 1988-05-10 | 1991-09-19 | イー・アイ・デユポン・ド・ネモアース・アンド・コンパニー | 迅速な核酸検出法 |
| WO1990002205A1 (fr) * | 1988-08-25 | 1990-03-08 | Angenics, Inc. | Detection de sequences d'acides nucleiques par agglutination de particules |
| US6235488B1 (en) | 1988-09-29 | 2001-05-22 | Agilent Technologies, Inc. | Surface preparation for chemical-specific binding |
| ATE118824T1 (de) | 1989-03-10 | 1995-03-15 | Amoco Corp | Immobilisierte oligonukleotidsonden und ihre verwendungen. |
| DE69027077T2 (de) * | 1989-09-08 | 1996-10-02 | Hewlett Packard Co | Herstellung einer Festphase, geeignet für spezifische Bindung chemischer Substanzen |
| GB8924989D0 (en) * | 1989-11-06 | 1989-12-28 | Scotgen Ltd | Method and device for the detection of antibiotic resistance |
| GB8926269D0 (en) * | 1989-11-21 | 1990-01-10 | Dynal As | Plasmid |
| DE4001154A1 (de) * | 1990-01-17 | 1991-07-18 | Boehringer Mannheim Gmbh | Verfahren zur herstellung von modifizierten nukleinsaeuren |
| US5556748A (en) * | 1991-07-30 | 1996-09-17 | Xenopore Corporation | Methods of sandwich hybridization for the quantitative analysis of oligonucleotides |
| ES2049618B1 (es) * | 1991-11-13 | 1994-11-01 | Consejo Superior Investigacion | Metodo de diagnostico y clasificacion de especies de trypanosoma cruzi. |
| EP0672174A4 (fr) * | 1991-12-23 | 1997-07-16 | Chiron Corp | Sondes d'htlv-1 utilises dans des methodes d'hybridation en sandwich en phase solution. |
| US5656432A (en) * | 1992-02-10 | 1997-08-12 | Bio Merieux | Genomic DNA fragment of Streptococcus pneumoniae, hybridization probe, amplification primer, reagent and method for the detection of Streptococcus pneumoniae |
| FR2687168B1 (fr) * | 1992-02-10 | 1994-03-25 | Bio Merieux | Fragment de l'adn genomique de streptococcus pneumoniae, sonde d'hybridation, amorce d'amplification, reactif et procede de detection de streptococcus pneumoniae. |
| US7713528B1 (en) | 1993-02-18 | 2010-05-11 | Enzo Therapeutics, Inc. | Method for in vivo delivery of active compounds using reagent conjugate |
| US5445936A (en) * | 1993-09-15 | 1995-08-29 | Ciba Corning Diagnostics Corp. | Method for non-competitive binding assays |
| US6203978B1 (en) * | 1996-05-31 | 2001-03-20 | Du Vergier Ltd. | Capture of single stranded nucleic acids |
| US20060275782A1 (en) | 1999-04-20 | 2006-12-07 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
| US7611869B2 (en) | 2000-02-07 | 2009-11-03 | Illumina, Inc. | Multiplexed methylation detection methods |
| US7955794B2 (en) | 2000-09-21 | 2011-06-07 | Illumina, Inc. | Multiplex nucleic acid reactions |
| US8076063B2 (en) | 2000-02-07 | 2011-12-13 | Illumina, Inc. | Multiplexed methylation detection methods |
| US7582420B2 (en) | 2001-07-12 | 2009-09-01 | Illumina, Inc. | Multiplex nucleic acid reactions |
| WO2003048732A2 (fr) * | 2001-02-07 | 2003-06-12 | Illumina, Inc. | Procedes multiplexes de detection de methylation |
| DE602004022943D1 (de) | 2003-05-30 | 2009-10-15 | Nanosphere Inc | Verfahren zum nachweis von analyten auf grundlage von evaneszenzbelichtung und auf streuung beruhender nachweis von nanopartikelsondenkomplexen |
| CN102822352B (zh) * | 2010-03-31 | 2015-07-29 | 有限会社山口技术特许机构 | 利用核酸色谱法的肺炎病原菌的检出方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2422956A1 (fr) * | 1978-04-13 | 1979-11-09 | Pasteur Institut | Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede |
| CA1219824A (fr) * | 1981-04-17 | 1987-03-31 | David C. Ward | Nucleotides modifies, leur preparation et leur emploi |
-
1984
- 1984-09-25 CA CA000463935A patent/CA1256005A/fr not_active Expired
- 1984-09-25 EP EP84306513A patent/EP0139489A3/fr not_active Withdrawn
- 1984-09-25 NO NO843838A patent/NO843838L/no unknown
- 1984-09-26 AU AU33554/84A patent/AU3355484A/en not_active Abandoned
- 1984-09-26 JP JP59199665A patent/JPS6093355A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CA1256005A (fr) | 1989-06-20 |
| JPS6093355A (ja) | 1985-05-25 |
| EP0139489A2 (fr) | 1985-05-02 |
| EP0139489A3 (fr) | 1988-01-13 |
| AU3355484A (en) | 1986-09-11 |
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