NO861555L - Merkede og merkbare reagenser for fluorometriske analyser. - Google Patents

Merkede og merkbare reagenser for fluorometriske analyser.

Info

Publication number: NO861555L
Authority: NO; Norway
Prior art keywords: condensation product; substance; antibody; antigen; metal ion
Prior art date: 1984-08-22

Application number

NO861555A

Other languages

English (en)

Norwegian (no)

Inventor

Salifu Dakubu

Original Assignee

Ekins Roger Philip

Salifu Dakubu

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-08-22

Filing date

1986-04-18

Publication date

1986-04-18

1986-04-18 Application filed by Ekins Roger Philip, Salifu Dakubu filed Critical Ekins Roger Philip

1986-04-18 Publication of NO861555L publication Critical patent/NO861555L/no

Links

239000003153 chemical reaction reagent Substances 0.000 title claims description 28
238000004458 analytical method Methods 0.000 title claims description 19
239000000126 substance Substances 0.000 claims description 52
239000007859 condensation product Substances 0.000 claims description 45
229910021645 metal ion Inorganic materials 0.000 claims description 38
125000004122 cyclic group Chemical group 0.000 claims description 31
229920002521 macromolecule Polymers 0.000 claims description 31
125000002485 formyl group Chemical class [H]C(*)=O 0.000 claims description 27
238000000034 method Methods 0.000 claims description 27
239000000427 antigen Substances 0.000 claims description 26
102000036639 antigens Human genes 0.000 claims description 26
108091007433 antigens Proteins 0.000 claims description 26
229910052747 lanthanoid Inorganic materials 0.000 claims description 25
150000002602 lanthanoids Chemical class 0.000 claims description 24
WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical group O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 14
230000000295 complement effect Effects 0.000 claims description 14
239000000047 product Substances 0.000 claims description 12
150000001875 compounds Chemical class 0.000 claims description 10
238000002372 labelling Methods 0.000 claims description 9
230000003389 potentiating effect Effects 0.000 claims description 7
108010039918 Polylysine Proteins 0.000 claims description 6
239000013522 chelant Substances 0.000 claims description 6
239000007795 chemical reaction product Substances 0.000 claims description 6
125000005594 diketone group Chemical group 0.000 claims description 6
229920000656 polylysine Polymers 0.000 claims description 6
239000000872 buffer Substances 0.000 claims description 5
229910052771 Terbium Inorganic materials 0.000 claims 2
LNBHUCHAFZUEGJ-UHFFFAOYSA-N europium(3+) Chemical compound [Eu+3] LNBHUCHAFZUEGJ-UHFFFAOYSA-N 0.000 claims 2
238000000605 extraction Methods 0.000 claims 2
GZCRRIHWUXGPOV-UHFFFAOYSA-N terbium atom Chemical compound [Tb] GZCRRIHWUXGPOV-UHFFFAOYSA-N 0.000 claims 2
ABBQHOQBGMUPJH-UHFFFAOYSA-M Sodium salicylate Chemical compound [Na+].OC1=CC=CC=C1C([O-])=O ABBQHOQBGMUPJH-UHFFFAOYSA-M 0.000 claims 1
229930189603 alysine Natural products 0.000 claims 1
238000006243 chemical reaction Methods 0.000 description 12
229910052751 metal Inorganic materials 0.000 description 12
239000002184 metal Substances 0.000 description 12
239000000243 solution Substances 0.000 description 9
238000003556 assay Methods 0.000 description 5
239000007787 solid Substances 0.000 description 5
SHXHPUAKLCCLDV-UHFFFAOYSA-N 1,1,1-trifluoropentane-2,4-dione Chemical compound CC(=O)CC(=O)C(F)(F)F SHXHPUAKLCCLDV-UHFFFAOYSA-N 0.000 description 4
239000008351 acetate buffer Substances 0.000 description 4
-1 alkyl aldehydes Chemical class 0.000 description 4
230000015572 biosynthetic process Effects 0.000 description 4
239000010949 copper Substances 0.000 description 4
238000003018 immunoassay Methods 0.000 description 4
102000004169 proteins and genes Human genes 0.000 description 4
108090000623 proteins and genes Proteins 0.000 description 4
QAEDZJGFFMLHHQ-UHFFFAOYSA-N trifluoroacetic anhydride Chemical compound FC(F)(F)C(=O)OC(=O)C(F)(F)F QAEDZJGFFMLHHQ-UHFFFAOYSA-N 0.000 description 4
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
241000283973 Oryctolagus cuniculus Species 0.000 description 3
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
210000001124 body fluid Anatomy 0.000 description 3
239000010839 body fluid Substances 0.000 description 3
238000011088 calibration curve Methods 0.000 description 3
239000003446 ligand Substances 0.000 description 3
RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
102000004190 Enzymes Human genes 0.000 description 2
108090000790 Enzymes Proteins 0.000 description 2
241001465754 Metazoa Species 0.000 description 2
102000004338 Transferrin Human genes 0.000 description 2
108090000901 Transferrin Proteins 0.000 description 2
125000003118 aryl group Chemical group 0.000 description 2
230000009920 chelation Effects 0.000 description 2
229910052802 copper Inorganic materials 0.000 description 2
230000007812 deficiency Effects 0.000 description 2
150000002148 esters Chemical class 0.000 description 2
238000007421 fluorometric assay Methods 0.000 description 2
239000008098 formaldehyde solution Substances 0.000 description 2
239000005556 hormone Substances 0.000 description 2
229940088597 hormone Drugs 0.000 description 2
150000002500 ions Chemical class 0.000 description 2
238000005259 measurement Methods 0.000 description 2
238000003127 radioimmunoassay Methods 0.000 description 2
239000011780 sodium chloride Substances 0.000 description 2
125000001424 substituent group Chemical group 0.000 description 2
239000000758 substrate Substances 0.000 description 2
ZMBHCYHQLYEYDV-UHFFFAOYSA-N trioctylphosphine oxide Chemical compound CCCCCCCCP(=O)(CCCCCCCC)CCCCCCCC ZMBHCYHQLYEYDV-UHFFFAOYSA-N 0.000 description 2
QLCMAPLSHSKAKP-UHFFFAOYSA-N 1,1,1-trifluoro-3-(thiophene-2-carbonyl)pentane-2,4-dione Chemical compound FC(F)(F)C(=O)C(C(=O)C)C(=O)C1=CC=CS1 QLCMAPLSHSKAKP-UHFFFAOYSA-N 0.000 description 1
LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide Substances CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 description 1
125000002941 2-furyl group Chemical group O1C([*])=C([H])C([H])=C1[H] 0.000 description 1
FPQQSJJWHUJYPU-UHFFFAOYSA-N 3-(dimethylamino)propyliminomethylidene-ethylazanium;chloride Chemical compound Cl.CCN=C=NCCCN(C)C FPQQSJJWHUJYPU-UHFFFAOYSA-N 0.000 description 1
UYQMAGRFYJIJOQ-UHFFFAOYSA-N 4,4,4-trifluoro-1-naphthalen-1-ylbutane-1,3-dione Chemical compound C1=CC=C2C(C(=O)CC(=O)C(F)(F)F)=CC=CC2=C1 UYQMAGRFYJIJOQ-UHFFFAOYSA-N 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical class OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
239000004472 Lysine Substances 0.000 description 1
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 1
238000010521 absorption reaction Methods 0.000 description 1
238000000862 absorption spectrum Methods 0.000 description 1
230000002378 acidificating effect Effects 0.000 description 1
150000001412 amines Chemical class 0.000 description 1
125000000539 amino acid group Chemical group 0.000 description 1
150000001413 amino acids Chemical class 0.000 description 1
125000003277 amino group Chemical group 0.000 description 1
150000001540 azides Chemical class 0.000 description 1
125000003236 benzoyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C(*)=O 0.000 description 1
230000001588 bifunctional effect Effects 0.000 description 1
150000001718 carbodiimides Chemical class 0.000 description 1
239000002738 chelating agent Substances 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
239000003431 cross linking reagent Substances 0.000 description 1
238000001514 detection method Methods 0.000 description 1
238000010586 diagram Methods 0.000 description 1
238000000502 dialysis Methods 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
201000010099 disease Diseases 0.000 description 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
239000003814 drug Substances 0.000 description 1
229940079593 drug Drugs 0.000 description 1
229940088598 enzyme Drugs 0.000 description 1
238000011156 evaluation Methods 0.000 description 1
230000005284 excitation Effects 0.000 description 1
239000012467 final product Substances 0.000 description 1
238000011534 incubation Methods 0.000 description 1
ZBKFYXZXZJPWNQ-UHFFFAOYSA-N isothiocyanate group Chemical group [N-]=C=S ZBKFYXZXZJPWNQ-UHFFFAOYSA-N 0.000 description 1
239000000463 material Substances 0.000 description 1
150000002739 metals Chemical class 0.000 description 1
WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
238000012986 modification Methods 0.000 description 1
230000004048 modification Effects 0.000 description 1
238000009206 nuclear medicine Methods 0.000 description 1
239000002245 particle Substances 0.000 description 1
229920000729 poly(L-lysine) polymer Polymers 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
239000002243 precursor Substances 0.000 description 1
238000000746 purification Methods 0.000 description 1
238000004451 qualitative analysis Methods 0.000 description 1
238000004445 quantitative analysis Methods 0.000 description 1
230000002285 radioactive effect Effects 0.000 description 1
150000003384 small molecules Chemical class 0.000 description 1
241000894007 species Species 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
239000007858 starting material Substances 0.000 description 1
239000011550 stock solution Substances 0.000 description 1
230000008685 targeting Effects 0.000 description 1
TXBBUSUXYMIVOS-UHFFFAOYSA-N thenoyltrifluoroacetone Chemical compound FC(F)(F)C(=O)CC(=O)C1=CC=CS1 TXBBUSUXYMIVOS-UHFFFAOYSA-N 0.000 description 1
231100000331 toxic Toxicity 0.000 description 1
230000002588 toxic effect Effects 0.000 description 1
239000012581 transferrin Substances 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
230000003612 virological effect Effects 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/805—Optical property
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/808—Automated or kit
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/802—Chromogenic or luminescent peptides

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Immunology (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
Chemical & Material Sciences (AREA)
Hematology (AREA)
Urology & Nephrology (AREA)
Food Science & Technology (AREA)
Biochemistry (AREA)
Cell Biology (AREA)
Biotechnology (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Analytical Chemistry (AREA)
Microbiology (AREA)
General Health & Medical Sciences (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Investigating Or Analysing Biological Materials (AREA)
Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Luminescent Compositions (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Peptides Or Proteins (AREA)

NO861555A 1984-08-22 1986-04-18 Merkede og merkbare reagenser for fluorometriske analyser. NO861555L (no)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
GB848421318A GB8421318D0 (en)	1984-08-22	1984-08-22	Potentiating antibodies/other macro-molecules

Publications (1)

Publication Number	Publication Date
NO861555L true NO861555L (no)	1986-04-18

Family

ID=10565671

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO861555A NO861555L (no)	1984-08-22	1986-04-18	Merkede og merkbare reagenser for fluorometriske analyser.

Country Status (10)

Country	Link
US (1)	US4857475A (da)
EP (1)	EP0222766B1 (da)
JP (1)	JPH0625765B2 (da)
AU (1)	AU4724185A (da)
DE (1)	DE3583507D1 (da)
DK (1)	DK184586D0 (da)
FI (1)	FI87022C (da)
GB (1)	GB8421318D0 (da)
NO (1)	NO861555L (da)
WO (1)	WO1986001604A1 (da)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4886744A (en) *	1985-04-25	1989-12-12	Polaroid Corporation	Fluorescent conjugates and biological diagnostic assay system
GB8602304D0 (en) *	1986-01-30	1986-03-05	Dakubu S	Dihydropyridine condensation products
GB8622855D0 (en) *	1986-09-23	1986-10-29	Ekins R P	Determining biological substance
US5089423A (en) *	1987-05-06	1992-02-18	Cyberfluor Inc.	Immunoassay methods and reagents and methods for producing the latter
GB8803000D0 (en) *	1988-02-10	1988-03-09	Ekins Roger Philip	Determination of ambient concentrations of several analytes
SE8703682L (sv) *	1987-09-24	1989-03-25	Wallac Oy	Homogen bestaemningsmetod som utnyttjar affinitetsreaktioner
US5308754A (en) *	1988-03-21	1994-05-03	Kankare Jouko J	Electrogenerated luminescence in solution
SE8801702D0 (sv) *	1988-05-05	1988-05-05	Pharmacia Ab	Sett vid bestemning av enzymatisk aktivitet
US5240867A (en) *	1989-02-09	1993-08-31	Fujitsu Limited	Semiconductor integrated circuit having interconnection with improved design flexibility, and method of production
FI88654C (fi) *	1991-03-15	1993-06-10	Datacity Center Oy	Fluorescenshoejningsmetod
US5312922A (en) *	1992-04-06	1994-05-17	Nordion International Inc.	Europium and terbium chelators for time-resolved fluorometric assays
GB0210535D0 (en) *	2002-05-08	2002-06-19	Novartis Ag	Organic compounds
US7906343B2 (en) *	2005-01-24	2011-03-15	Sri International	Surface-enhanced lanthanide chelates

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US3956341A (en) *	1974-02-21	1976-05-11	Smithkline Corporation	1,3,5-Tricarbo-1,4-dihydropyridines
SE428332B (sv) *	1979-03-08	1983-06-20	Wallac Oy	Forfarande for fluorescensspektroskopisk bestemning av biologiskt aktivt emne, sasom hapten, antikropp eller antigen
US4352751A (en) *	1979-09-10	1982-10-05	Analytical Radiation Corporation	Species-linked diamine triacetic acids and their chelates

1984
- 1984-08-22 GB GB848421318A patent/GB8421318D0/en active Pending
1985
- 1985-08-22 WO PCT/GB1985/000377 patent/WO1986001604A1/en not_active Ceased
- 1985-08-22 DE DE8585904129T patent/DE3583507D1/de not_active Expired - Fee Related
- 1985-08-22 EP EP85904129A patent/EP0222766B1/en not_active Expired
- 1985-08-22 JP JP60503723A patent/JPH0625765B2/ja not_active Expired - Lifetime
- 1985-08-22 US US06/862,215 patent/US4857475A/en not_active Expired - Lifetime
- 1985-08-22 AU AU47241/85A patent/AU4724185A/en not_active Abandoned
1986
- 1986-04-18 NO NO861555A patent/NO861555L/no unknown
- 1986-04-22 DK DK184586A patent/DK184586D0/da not_active Application Discontinuation
1987
- 1987-02-20 FI FI870716A patent/FI87022C/fi not_active IP Right Cessation

Also Published As

Publication number	Publication date
WO1986001604A1 (en)	1986-03-13
JPS62501442A (ja)	1987-06-11
FI87022C (fi)	1992-11-10
DK184586A (da)	1986-04-22
JPH0625765B2 (ja)	1994-04-06
DE3583507D1 (de)	1991-08-22
EP0222766B1 (en)	1991-07-17
EP0222766A1 (en)	1987-05-27
DK184586D0 (da)	1986-04-22
FI87022B (fi)	1992-07-31
GB8421318D0 (en)	1984-09-26
FI870716L (fi)	1987-02-20
FI870716A0 (fi)	1987-02-20
US4857475A (en)	1989-08-15
AU4724185A (en)	1986-03-24

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