NO983102L - Uttrykk og forsterkning av sekvenselementer (EASE) for eukaryote ekspresjonssystemer - Google Patents

Uttrykk og forsterkning av sekvenselementer (EASE) for eukaryote ekspresjonssystemer

Info

Publication number
NO983102L
NO983102L NO983102A NO983102A NO983102L NO 983102 L NO983102 L NO 983102L NO 983102 A NO983102 A NO 983102A NO 983102 A NO983102 A NO 983102A NO 983102 L NO983102 L NO 983102L
Authority
NO
Norway
Prior art keywords
expression
amplification
ease
sequence elements
eukaryotic
Prior art date
Application number
NO983102A
Other languages
English (en)
Other versions
NO983102D0 (no
NO323277B1 (no
Inventor
Arvia E Morris
Chi-Chang Lee
James N Thomas
Original Assignee
Immunex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immunex Corp filed Critical Immunex Corp
Publication of NO983102D0 publication Critical patent/NO983102D0/no
Publication of NO983102L publication Critical patent/NO983102L/no
Publication of NO323277B1 publication Critical patent/NO323277B1/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/30—Vector systems having a special element relevant for transcription being an enhancer not forming part of the promoter region
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Control Of Indicators Other Than Cathode Ray Tubes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Amplifiers (AREA)
  • Error Detection And Correction (AREA)
NO19983102A 1996-01-11 1998-07-03 Ekspresjonsokende sekvenselementer (EASE) for eukaryote ekspresjonssystemer NO323277B1 (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US58650996A 1996-01-11 1996-01-11
PCT/US1997/000483 WO1997025420A1 (en) 1996-01-11 1997-01-10 Expression augmenting sequence elements (ease) for eukaryotic expression systems

Publications (3)

Publication Number Publication Date
NO983102D0 NO983102D0 (no) 1998-07-03
NO983102L true NO983102L (no) 1998-09-08
NO323277B1 NO323277B1 (no) 2007-02-26

Family

ID=24346030

Family Applications (1)

Application Number Title Priority Date Filing Date
NO19983102A NO323277B1 (no) 1996-01-11 1998-07-03 Ekspresjonsokende sekvenselementer (EASE) for eukaryote ekspresjonssystemer

Country Status (14)

Country Link
US (3) US6027915A (no)
EP (1) EP0873405B1 (no)
JP (1) JP3495375B2 (no)
KR (1) KR19990077015A (no)
AT (1) ATE275628T1 (no)
AU (1) AU708981B2 (no)
CA (1) CA2241174C (no)
DE (1) DE69730592T2 (no)
DK (1) DK0873405T3 (no)
ES (1) ES2227669T3 (no)
NO (1) NO323277B1 (no)
NZ (1) NZ330926A (no)
PT (1) PT873405E (no)
WO (1) WO1997025420A1 (no)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3495375B2 (ja) * 1996-01-11 2004-02-09 イミュネックス・コーポレーション 真核生物発現システムの発現増大配列エレメント（ｅａｓｅ）
US6596514B2 (en) * 1996-01-11 2003-07-22 Immunex Corporation Expression augmenting sequence elements (EASE) for eukaryotic expression systems
PT951551E (pt) 1996-12-23 2008-10-28 Immunex Corp Ligando para receptor activador de nf-kb, ligando membro da superfamília de tnf
US6800457B2 (en) 1998-09-22 2004-10-05 Bristol-Myers Squibb Company Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
US6521419B1 (en) 1998-09-22 2003-02-18 Kanakaraju Koduri Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
DK1222298T3 (da) 1999-10-13 2006-04-10 Immunex Corp Vektorer og fremgangsmåder til rekombinant proteinekspression
EP2295079A3 (en) 1999-12-23 2011-03-23 ZymoGenetics, L.L.C. Method for treating inflammation
KR100450266B1 (ko) * 2002-01-16 2004-09-30 주식회사 엘지생명과학 유전자 발현 동물세포 제조용 바이시스트로닉 벡터 및이를 포함하는 세포주 그리고 이를 이용한 항체단백질의생산방법
WO2003087393A2 (en) * 2002-04-09 2003-10-23 Global Biotech Inc. Human tissue urokinase type plasminogen activator production
US20040110295A1 (en) * 2002-05-28 2004-06-10 Maxygen, Inc., A Delaware Corporation Nucleic acid vectors
JP2005031812A (ja) * 2003-07-08 2005-02-03 Toshiba Corp 画像形成装置およびその制御方法
KR100807312B1 (ko) 2005-10-22 2008-02-28 (주)리즈바이오텍 재조합 인간 인슐린 유사 성장 인자 결합 단백질-3의 생산정제 방법
US20080124760A1 (en) 2006-07-26 2008-05-29 Barbara Enenkel Regulatory Nucleic Acid Elements
EP2500414A1 (en) 2006-09-13 2012-09-19 Abbott Laboratories Cell culture improvements
MX353340B (es) 2006-09-13 2018-01-09 Abbvie Inc Mejoras de cultivos celulares.
WO2008121324A2 (en) 2007-03-30 2008-10-09 Abbott Laboratories Recombinant expression vector elements (reves) for enhancing expression of recombinant proteins in host cells
US8815794B2 (en) 2008-08-28 2014-08-26 The Research Foundation For The State University Of New York Treatment of amyloidoses using myelin basic protein and fragments thereof
JP4568378B2 (ja) * 2008-08-29 2010-10-27 東洋紡績株式会社 遺伝子発現安定化エレメント
WO2010062995A2 (en) 2008-11-26 2010-06-03 Five Prime Therapeutics, Inc. Compositions and methods for regulating collagen and smooth muscle actin expression by serpine2
EP2443239B1 (en) 2009-06-15 2015-11-25 Cellagenics B.V. Novel stringent selectable markers
US9790517B2 (en) 2010-06-15 2017-10-17 Cellagenics B.V. Intergenic elements for enhancing gene expression
KR101599138B1 (ko) 2014-06-17 2016-03-03 한국생명공학연구원 재조합 단백질 발현 증진을 위한 유전자 절편을 포함하는 벡터 및 이의 용도
WO2016003368A1 (en) * 2014-07-01 2016-01-07 Agency For Science, Technology And Research Optimized vectors for the production of recombinant proteins
EP3856910B1 (en) * 2018-09-24 2024-11-20 Merck Sharp & Dohme LLC Expression vectors for eukaryotic expression systems
WO2020123327A1 (en) 2018-12-10 2020-06-18 Amgen Inc. Mutated piggybac transposase
US20220073945A1 (en) 2018-12-21 2022-03-10 Merck Sharp & Dohme Corp. Expression vectors for eukaryotic expression systems
AU2020288407A1 (en) * 2019-06-07 2021-12-16 Biocon Biologics Limited Mammalian expression vectors
TW202233660A (zh) 2020-10-30 2022-09-01 美商安進公司 過表現胰島素樣生長因子受體突變體以調節ｉｇｆ補充
JP2024503239A (ja) 2020-12-22 2024-01-25 アムジェン インコーポレイテッド 細胞培養法
EP4351722A1 (en) 2021-06-10 2024-04-17 Amgen Inc. Engineered nrg-1 variants with improved selectivity toward erbb4 but not against erbb3
TW202328442A (zh) 2021-09-10 2023-07-16 美商安進公司 平臺宿主對ｉｇｆ—培養基之適應
KR20260042226A (ko) 2023-07-21 2026-03-30 암젠 인크 선택 가능한 마커 발현을 유도하는 프로모터의 조합을 포함하는 벡터
IL327356A (en) 2023-10-18 2026-05-01 Amgen Inc Quantitative assessment of mRNA transcript levels using DDPCR for initial pool characterization and evaluation of single-cell clones in the cell line development process
TW202546234A (zh) 2024-01-19 2025-12-01 美商安進公司 具有兩個輕鏈拷貝的表現載體系統
WO2025155774A2 (en) 2024-01-19 2025-07-24 Amgen Inc. Vector engineering strategies to enhance volumetric productivity and decrease impurity formation across a diverse set of modalities
WO2025184493A1 (en) 2024-02-29 2025-09-04 Amgen Inc. Dual selection expression vector systems for multi-chain biologics

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3730246A1 (de) * 1987-09-09 1989-06-01 Boehringer Mannheim Gmbh Expressionsvektor und verfahren zur expression von heterologen proteinen in saeugerzellen
JP3495375B2 (ja) * 1996-01-11 2004-02-09 イミュネックス・コーポレーション 真核生物発現システムの発現増大配列エレメント（ｅａｓｅ）

Also Published As

Publication number Publication date
AU708981B2 (en) 1999-08-19
DE69730592T2 (de) 2005-09-29
WO1997025420A1 (en) 1997-07-17
US6312951B1 (en) 2001-11-06
EP0873405B1 (en) 2004-09-08
AU1697397A (en) 1997-08-01
US6027915A (en) 2000-02-22
ATE275628T1 (de) 2004-09-15
EP0873405A4 (en) 2001-10-17
KR19990077015A (ko) 1999-10-25
US6309841B1 (en) 2001-10-30
CA2241174C (en) 2006-09-12
PT873405E (pt) 2005-01-31
ES2227669T3 (es) 2005-04-01
NZ330926A (en) 1999-11-29
JP2000503205A (ja) 2000-03-21
DK0873405T3 (da) 2005-01-17
EP0873405A1 (en) 1998-10-28
NO983102D0 (no) 1998-07-03
JP3495375B2 (ja) 2004-02-09
NO323277B1 (no) 2007-02-26
DE69730592D1 (de) 2004-10-14
CA2241174A1 (en) 1997-07-17

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