OA12620A - Viral drug susceptibility testing. - Google Patents

Viral drug susceptibility testing. Download PDF

Info

Publication number: OA12620A
Authority: OA; OAPI
Prior art keywords: enzyme; individual; drug; virus; sample
Prior art date: 2001-06-14

Application number

OA1200300318A

Other languages

English (en)

Inventor

Simon Gronowitz

Clas Kallander

Anders Malmsten

Xingwu Shao

Original Assignee

Cavidi Tech Ab

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2001-06-14

Filing date

2002-06-14

Publication date

2006-06-12

2002-06-14 Application filed by Cavidi Tech Ab filed Critical Cavidi Tech Ab

2006-06-12 Publication of OA12620A publication Critical patent/OA12620A/en

Links

229940079593 drug Drugs 0.000 title claims abstract description 67
239000003814 drug Substances 0.000 title claims abstract description 67
238000012360 testing method Methods 0.000 title claims abstract description 31
230000003612 virological effect Effects 0.000 title claims abstract description 21
102100034343 Integrase Human genes 0.000 claims abstract description 109
108010092799 RNA-directed DNA polymerase Proteins 0.000 claims abstract description 108
102000004190 Enzymes Human genes 0.000 claims abstract description 50
108090000790 Enzymes Proteins 0.000 claims abstract description 50
230000000694 effects Effects 0.000 claims abstract description 48
241000700605 Viruses Species 0.000 claims abstract description 39
239000000523 sample Substances 0.000 claims abstract description 21
238000000034 method Methods 0.000 claims abstract description 18
239000003112 inhibitor Substances 0.000 claims abstract description 15
239000003795 chemical substances by application Substances 0.000 claims abstract description 14
230000000415 inactivating effect Effects 0.000 claims abstract description 14
230000035945 sensitivity Effects 0.000 claims abstract description 13
238000002560 therapeutic procedure Methods 0.000 claims abstract description 8
238000011282 treatment Methods 0.000 claims abstract description 7
239000008280 blood Substances 0.000 claims abstract description 6
210000004369 blood Anatomy 0.000 claims abstract description 6
238000001952 enzyme assay Methods 0.000 claims abstract description 6
210000002845 virion Anatomy 0.000 claims abstract description 6
239000003443 antiviral agent Substances 0.000 claims abstract description 5
230000000903 blocking effect Effects 0.000 claims abstract description 5
239000012472 biological sample Substances 0.000 claims abstract description 4
230000002934 lysing effect Effects 0.000 claims abstract description 3
239000002245 particle Substances 0.000 claims abstract description 3
108010078851 HIV Reverse Transcriptase Proteins 0.000 claims abstract 2
241000725303 Human immunodeficiency virus Species 0.000 claims description 24
238000003556 assay Methods 0.000 claims description 17
241001430294 unidentified retrovirus Species 0.000 claims description 4
238000010998 test method Methods 0.000 abstract description 2
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238000002955 isolation Methods 0.000 description 13
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238000006467 substitution reaction Methods 0.000 description 11
241000713772 Human immunodeficiency virus 1 Species 0.000 description 10
239000002777 nucleoside Substances 0.000 description 10
229940124821 NNRTIs Drugs 0.000 description 9
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150000003833 nucleoside derivatives Chemical class 0.000 description 9
NQDJXKOVJZTUJA-UHFFFAOYSA-N nevirapine Chemical compound C12=NC=CC=C2C(=O)NC=2C(C)=CC=NC=2N1C1CC1 NQDJXKOVJZTUJA-UHFFFAOYSA-N 0.000 description 8
XPOQHMRABVBWPR-ZDUSSCGKSA-N efavirenz Chemical compound C([C@]1(C2=CC(Cl)=CC=C2NC(=O)O1)C(F)(F)F)#CC1CC1 XPOQHMRABVBWPR-ZDUSSCGKSA-N 0.000 description 7
229940127073 nucleoside analogue Drugs 0.000 description 7
239000011541 reaction mixture Substances 0.000 description 7
ODSQODTUNULBHF-JGVFFNPUSA-N 2',3'-dehydro-2',3'-deoxy-thymidine 5'-triphosphate Chemical compound O=C1NC(=O)C(C)=CN1[C@H]1C=C[C@@H](CO[P@@](O)(=O)O[P@](O)(=O)OP(O)(O)=O)O1 ODSQODTUNULBHF-JGVFFNPUSA-N 0.000 description 6
XPOQHMRABVBWPR-UHFFFAOYSA-N Efavirenz Natural products O1C(=O)NC2=CC=C(Cl)C=C2C1(C(F)(F)F)C#CC1CC1 XPOQHMRABVBWPR-UHFFFAOYSA-N 0.000 description 6
WHBIGIKBNXZKFE-UHFFFAOYSA-N delavirdine Chemical compound CC(C)NC1=CC=CN=C1N1CCN(C(=O)C=2NC3=CC=C(NS(C)(=O)=O)C=C3C=2)CC1 WHBIGIKBNXZKFE-UHFFFAOYSA-N 0.000 description 6
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PFNFFQXMRSDOHW-UHFFFAOYSA-N spermine Chemical compound NCCCNCCCCNCCCN PFNFFQXMRSDOHW-UHFFFAOYSA-N 0.000 description 6
BLQCQNFLEGAHPA-RRKCRQDMSA-N [[(2r,3s,5r)-5-(5-bromo-2,4-dioxopyrimidin-1-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C(Br)=C1 BLQCQNFLEGAHPA-RRKCRQDMSA-N 0.000 description 5
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UFULAYFCSOUIOV-UHFFFAOYSA-N cysteamine Chemical compound NCCS UFULAYFCSOUIOV-UHFFFAOYSA-N 0.000 description 4
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229960000689 nevirapine Drugs 0.000 description 4
238000006116 polymerization reaction Methods 0.000 description 4
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JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 3
KIUMMUBSPKGMOY-UHFFFAOYSA-N 3,3'-Dithiobis(6-nitrobenzoic acid) Chemical compound C1=C([N+]([O-])=O)C(C(=O)O)=CC(SSC=2C=C(C(=CC=2)[N+]([O-])=O)C(O)=O)=C1 KIUMMUBSPKGMOY-UHFFFAOYSA-N 0.000 description 3
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HBOMLICNUCNMMY-XLPZGREQSA-N zidovudine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](N=[N+]=[N-])C1 HBOMLICNUCNMMY-XLPZGREQSA-N 0.000 description 3
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KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
101900297506 Human immunodeficiency virus type 1 group M subtype B Reverse transcriptase/ribonuclease H Proteins 0.000 description 2
101710203526 Integrase Proteins 0.000 description 2
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
GLUUGHFHXGJENI-UHFFFAOYSA-N Piperazine Chemical compound C1CNCCN1 GLUUGHFHXGJENI-UHFFFAOYSA-N 0.000 description 2
QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 2
108020000999 Viral RNA Proteins 0.000 description 2
MCNGPQRQMPMJSB-MYINAIGISA-N [(2r,3s,5s)-5-bromo-5-(2,4-dioxopyrimidin-1-yl)-3-hydroxyoxolan-2-yl]methyl dihydrogen phosphate Chemical compound O1[C@H](COP(O)(O)=O)[C@@H](O)C[C@]1(Br)N1C(=O)NC(=O)C=C1 MCNGPQRQMPMJSB-MYINAIGISA-N 0.000 description 2
229940024606 amino acid Drugs 0.000 description 2
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239000002726 nonnucleoside reverse transcriptase inhibitor Substances 0.000 description 2
238000003199 nucleic acid amplification method Methods 0.000 description 2
SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 2
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229910021653 sulphate ion Inorganic materials 0.000 description 2
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XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
BCHIXGBGRHLSBE-UHFFFAOYSA-N (4-methyl-2-oxochromen-7-yl) dihydrogen phosphate Chemical compound C1=C(OP(O)(O)=O)C=CC2=C1OC(=O)C=C2C BCHIXGBGRHLSBE-UHFFFAOYSA-N 0.000 description 1
FSRLCMRWYUJTNT-UONOGXRCSA-N 1-[2-(3-acetyl-2-hydroxy-6-methoxy-phenyl)-cyclopropyl]-3-(5-cyano-pyridin-2-yl)-thiourea Chemical compound COC1=CC=C(C(C)=O)C(O)=C1[C@@H]1[C@H](NC(=S)NC=2N=CC(=CC=2)C#N)C1 FSRLCMRWYUJTNT-UONOGXRCSA-N 0.000 description 1
CMCBDXRRFKYBDG-UHFFFAOYSA-N 1-dodecoxydodecane Chemical compound CCCCCCCCCCCCOCCCCCCCCCCCC CMCBDXRRFKYBDG-UHFFFAOYSA-N 0.000 description 1
SLAMLWHELXOEJZ-UHFFFAOYSA-N 2-nitrobenzoic acid Chemical compound OC(=O)C1=CC=CC=C1[N+]([O-])=O SLAMLWHELXOEJZ-UHFFFAOYSA-N 0.000 description 1
JTEGQNOMFQHVDC-RQJHMYQMSA-N 4-amino-1-[(2s,5r)-2-(hydroxymethyl)-1,3-oxathiolan-5-yl]pyrimidin-2-one Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](CO)SC1 JTEGQNOMFQHVDC-RQJHMYQMSA-N 0.000 description 1
KIUMMUBSPKGMOY-UHFFFAOYSA-L 5-[(3-carboxylato-4-nitrophenyl)disulfanyl]-2-nitrobenzoate Chemical compound C1=C([N+]([O-])=O)C(C(=O)[O-])=CC(SSC=2C=C(C(=CC=2)[N+]([O-])=O)C([O-])=O)=C1 KIUMMUBSPKGMOY-UHFFFAOYSA-L 0.000 description 1
102100027211 Albumin Human genes 0.000 description 1
108010088751 Albumins Proteins 0.000 description 1
102000002260 Alkaline Phosphatase Human genes 0.000 description 1
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101100421200 Caenorhabditis elegans sep-1 gene Proteins 0.000 description 1
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241000588724 Escherichia coli Species 0.000 description 1
206010053317 Hydrophobia Diseases 0.000 description 1
108010061833 Integrases Proteins 0.000 description 1
FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
229920002274 Nalgene Polymers 0.000 description 1
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108020004511 Recombinant DNA Proteins 0.000 description 1
238000012300 Sequence Analysis Methods 0.000 description 1
WPMWEFXCIYCJSA-UHFFFAOYSA-N Tetraethylene glycol monododecyl ether Chemical compound CCCCCCCCCCCCOCCOCCOCCOCCO WPMWEFXCIYCJSA-UHFFFAOYSA-N 0.000 description 1
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JXLHNMVSKXFWAO-UHFFFAOYSA-N azane;7-fluoro-2,1,3-benzoxadiazole-4-sulfonic acid Chemical compound N.OS(=O)(=O)C1=CC=C(F)C2=NON=C12 JXLHNMVSKXFWAO-UHFFFAOYSA-N 0.000 description 1
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238000001514 detection method Methods 0.000 description 1
239000003599 detergent Substances 0.000 description 1
CVLAEJNQNKXNNN-UHFFFAOYSA-N diazepin-4-one Chemical compound O=C1C=CC=NN=C1 CVLAEJNQNKXNNN-UHFFFAOYSA-N 0.000 description 1
229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 1
239000000890 drug combination Substances 0.000 description 1
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229920002113 octoxynol Polymers 0.000 description 1
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239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
239000012071 phase Substances 0.000 description 1
238000012247 phenotypical assay Methods 0.000 description 1
108700004029 pol Genes Proteins 0.000 description 1
101150088264 pol gene Proteins 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
238000003752 polymerase chain reaction Methods 0.000 description 1
239000001205 polyphosphate Substances 0.000 description 1
235000011176 polyphosphates Nutrition 0.000 description 1
235000011056 potassium acetate Nutrition 0.000 description 1
238000002203 pretreatment Methods 0.000 description 1
230000008569 process Effects 0.000 description 1
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125000004076 pyridyl group Chemical group 0.000 description 1
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230000004044 response Effects 0.000 description 1
238000010839 reverse transcription Methods 0.000 description 1
238000012552 review Methods 0.000 description 1
102200050020 rs1554988032 Human genes 0.000 description 1
102220028975 rs398123345 Human genes 0.000 description 1
102200069353 rs8103142 Human genes 0.000 description 1
238000012216 screening Methods 0.000 description 1
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239000001226 triphosphate Substances 0.000 description 1
235000011178 triphosphate Nutrition 0.000 description 1
UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
239000013598 vector Substances 0.000 description 1
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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
- G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
- G01N2333/16—HIV-1, HIV-2
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- G01N2333/91205—Phosphotransferases in general
- G01N2333/91245—Nucleotidyltransferases (2.7.7)
- G01N2333/9125—Nucleotidyltransferases (2.7.7) with a definite EC number (2.7.7.-)
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Biochemistry (AREA)
Physics & Mathematics (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

OA1200300318A 2001-06-14 2002-06-14 Viral drug susceptibility testing. OA12620A (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US29776401P	2001-06-14	2001-06-14

Publications (1)

Publication Number	Publication Date
OA12620A true OA12620A (en)	2006-06-12

Family

ID=23147646

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
OA1200300318A OA12620A (en)	2001-06-14	2002-06-14	Viral drug susceptibility testing.

Country Status (17)

Country	Link
US (1)	US7875422B2 (es)
EP (1)	EP1395676B1 (es)
JP (1)	JP4455054B2 (es)
CN (1)	CN1539022B (es)
AP (1)	AP1592A (es)
AT (1)	ATE316150T1 (es)
AU (1)	AU2002309447B2 (es)
BR (1)	BRPI0210361B8 (es)
DE (1)	DE60208788T2 (es)
EA (1)	EA006161B1 (es)
ES (1)	ES2257553T3 (es)
MX (1)	MXPA03011564A (es)
OA (1)	OA12620A (es)
PL (1)	PL209075B1 (es)
PT (1)	PT1395676E (es)
WO (1)	WO2002103040A1 (es)
ZA (1)	ZA200309551B (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP2016523088A (ja) *	2013-06-26	2016-08-08	フィロジカリミテッドＰｈｙｌｏｇｉｃａＬｉｍｉｔｅｄ	ペプチドの細胞輸送をモニタリングする方法
BR112019027993A2 (pt) *	2017-07-04	2020-07-07	Cavidi Ab	métodos para avaliar a susceptibilidade de um vírus ao tratamento com um fármaco inibidor de uma enzima do vírus do tipo selvagem, para avaliar se um paciente tratado para uma infecção viral tem necessidade de alterar a terapia farmacológica com um fármaco inibidor da enzima viral e para determinar a carga de um vírus em uma amostra do paciente e a dita resistência do vírus ao tratamento com um fármaco inibidor de uma enzima do vírus do tipo selvagem, e, sistema
CN108896759B (zh) *	2018-06-27	2020-05-19	南京医科大学	受试物致敏性检测方法及其试剂盒

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* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
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2002
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Publication number	Publication date
EP1395676B1 (en)	2006-01-18
AU2002309447B2 (en)	2007-05-24
JP4455054B2 (ja)	2010-04-21
BR0210361A (pt)	2004-06-22
PT1395676E (pt)	2006-06-30
AP2003002923A0 (en)	2003-12-31
BR0210361B1 (pt)	2014-12-30
ZA200309551B (en)	2005-02-23
PL367829A1 (en)	2005-03-07
EA006161B1 (ru)	2005-10-27
AP1592A (en)	2006-03-20
EA200301244A1 (ru)	2004-06-24
DE60208788D1 (de)	2006-04-06
JP2004530433A (ja)	2004-10-07
BRPI0210361B8 (pt)	2021-07-27
CN1539022B (zh)	2011-04-13
HK1065070A1 (en)	2005-02-08
US20040170958A1 (en)	2004-09-02
ATE316150T1 (de)	2006-02-15
CN1539022A (zh)	2004-10-20
ES2257553T3 (es)	2006-08-01
EP1395676A1 (en)	2004-03-10
US7875422B2 (en)	2011-01-25
WO2002103040A1 (en)	2002-12-27
MXPA03011564A (es)	2004-03-18
DE60208788T2 (de)	2006-11-09
PL209075B1 (pl)	2011-07-29

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